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The map label for this gene is queF [H]

Identifier: 16273205

GI number: 16273205

Start: 1372222

End: 1373061

Strand: Reverse

Name: queF [H]

Synonym: HI1291

Alternate gene names: 16273205

Gene position: 1373061-1372222 (Counterclockwise)

Preceding gene: 16273206

Following gene: 30995437

Centisome position: 75.02

GC content: 37.74

Gene sequence:

>840_bases
ATGAATTACCAAGACAACAGCTTAAAATCCTTAAAACTCGGTCAAAAAACGGAATATGCCTCTCAATATGACCGCACTTT
ATTACAGCCAGTGCCACGCGCGTTAAATCGTGATGGATTGGGAATTACTCAAAACCAACCATTTACTATCGGCGCCGATA
TTTGGACGGCTTATGAAATCTCGTGGCTCAACGAAAAAGGCTTACCGCAAGTGGCTATCGCAGATATTTATCTGGATTAT
CAAAGCCAAAATTTGATTGAATCAAAAAGTTTTAAACTTTATTTAAATAGCTTTAACCAAAGCAAATTTGCCGACTTCAA
CGCCGTTCAACAAACTATGCAACGTGATTTAAGCGAATGTGCGCAAGGCGATGTAAAAGTGCGGTTAAATCCAATGGCAG
TTTATGATTCTCAAAAAATTGATCATTTACAAGGCGATTGCATTGATGAACAAGATATTGAAATTACTAGCTACGAATTT
AACGCTAATTGGCTGAAAGATTGCGTATCGGATGAAATTGTCGAAGAAAAATTAGTCAGCCATTTATTGAAATCTAACTG
CCTCATTACCAATCAACCAGACTGGGGCACCTTGCATATTCACTATGTTGGTAAAAAAATCAATCAGGAAAAATTATTGC
GTTACGTTGTATCGTTCCGCCAACATAATGAATTCCACGAACAATGTGTCGAACGTATTTTTTGCGATTTAATGCACTAC
GCAAAACCAGAAAAACTCACCGTTTACGCACGCTATACCCGTCGTGGCGGATTAGACATTAATCCATTCCGCTCTAATTT
TGAGAATTTACCTGAAAATTTAAGATTAGCGAGACAGTAA

Upstream 100 bases:

>100_bases
TGGAATGATAAAGTCTTAGCCCCCTTCCGACGCTTCGCGCCACCTTCCCCCGCAAGCAAGGGAAGGCAAAATTATTTTTT
AACATATAAAGGAAAAATAA

Downstream 100 bases:

>100_bases
AAAACTGCGGTGAAATTTCACCGCACTTTTCTCTACCCTTGTTTCAAATCATTTGCCTGTTGCAATAGCTGACGGCTTTC
TTTTAAAAATTGTTCGGAAT

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase [H]

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MNYQDNSLKSLKLGQKTEYASQYDRTLLQPVPRALNRDGLGITQNQPFTIGADIWTAYEISWLNEKGLPQVAIADIYLDY
QSQNLIESKSFKLYLNSFNQSKFADFNAVQQTMQRDLSECAQGDVKVRLNPMAVYDSQKIDHLQGDCIDEQDIEITSYEF
NANWLKDCVSDEIVEEKLVSHLLKSNCLITNQPDWGTLHIHYVGKKINQEKLLRYVVSFRQHNEFHEQCVERIFCDLMHY
AKPEKLTVYARYTRRGGLDINPFRSNFENLPENLRLARQ

Sequences:

>Translated_279_residues
MNYQDNSLKSLKLGQKTEYASQYDRTLLQPVPRALNRDGLGITQNQPFTIGADIWTAYEISWLNEKGLPQVAIADIYLDY
QSQNLIESKSFKLYLNSFNQSKFADFNAVQQTMQRDLSECAQGDVKVRLNPMAVYDSQKIDHLQGDCIDEQDIEITSYEF
NANWLKDCVSDEIVEEKLVSHLLKSNCLITNQPDWGTLHIHYVGKKINQEKLLRYVVSFRQHNEFHEQCVERIFCDLMHY
AKPEKLTVYARYTRRGGLDINPFRSNFENLPENLRLARQ
>Mature_279_residues
MNYQDNSLKSLKLGQKTEYASQYDRTLLQPVPRALNRDGLGITQNQPFTIGADIWTAYEISWLNEKGLPQVAIADIYLDY
QSQNLIESKSFKLYLNSFNQSKFADFNAVQQTMQRDLSECAQGDVKVRLNPMAVYDSQKIDHLQGDCIDEQDIEITSYEF
NANWLKDCVSDEIVEEKLVSHLLKSNCLITNQPDWGTLHIHYVGKKINQEKLLRYVVSFRQHNEFHEQCVERIFCDLMHY
AKPEKLTVYARYTRRGGLDINPFRSNFENLPENLRLARQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) [H]

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789158, Length=275, Percent_Identity=61.8181818181818, Blast_Score=351, Evalue=3e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016428
- InterPro:   IPR020602 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =1.7.1.13 [H]

Molecular weight: Translated: 32543; Mature: 32543

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYQDNSLKSLKLGQKTEYASQYDRTLLQPVPRALNRDGLGITQNQPFTIGADIWTAYEI
CCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCEEEEEE
SWLNEKGLPQVAIADIYLDYQSQNLIESKSFKLYLNSFNQSKFADFNAVQQTMQRDLSEC
EECCCCCCCCEEEEEEEEECCCCCHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
AQGDVKVRLNPMAVYDSQKIDHLQGDCIDEQDIEITSYEFNANWLKDCVSDEIVEEKLVS
HCCCEEEEECCEEEECCCHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHH
HLLKSNCLITNQPDWGTLHIHYVGKKINQEKLLRYVVSFRQHNEFHEQCVERIFCDLMHY
HHHHCCCEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
AKPEKLTVYARYTRRGGLDINPFRSNFENLPENLRLARQ
CCCCEEEEEEEEECCCCCCCCHHHHHHHHCCHHHHHCCC
>Mature Secondary Structure
MNYQDNSLKSLKLGQKTEYASQYDRTLLQPVPRALNRDGLGITQNQPFTIGADIWTAYEI
CCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCEEEEEE
SWLNEKGLPQVAIADIYLDYQSQNLIESKSFKLYLNSFNQSKFADFNAVQQTMQRDLSEC
EECCCCCCCCEEEEEEEEECCCCCHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
AQGDVKVRLNPMAVYDSQKIDHLQGDCIDEQDIEITSYEFNANWLKDCVSDEIVEEKLVS
HCCCEEEEECCEEEECCCHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHH
HLLKSNCLITNQPDWGTLHIHYVGKKINQEKLLRYVVSFRQHNEFHEQCVERIFCDLMHY
HHHHCCCEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
AKPEKLTVYARYTRRGGLDINPFRSNFENLPENLRLARQ
CCCCEEEEEEEEECCCCCCCCHHHHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA