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The map label for this gene is purB [H]

Identifier: 99080852

GI number: 99080852

Start: 1082157

End: 1083461

Strand: Reverse

Name: purB [H]

Synonym: TM1040_1011

Alternate gene names: 99080852

Gene position: 1083461-1082157 (Counterclockwise)

Preceding gene: 99080853

Following gene: 99080851

Centisome position: 33.85

GC content: 61.23

Gene sequence:

>1305_bases
ATGATCCCCCGCTACGCCCGCCCCGATATGGTTGCCGTCTGGTCTCCCGAGACCAAGTTCCGCATCTGGTACGAAATCGA
GGCGCACGCCTGCGACGCCATGGCAAACCTCGGCGTGATCCCGCGCGAGAATGCCGAAGCGGTCTGGAAAGCCAAAGATG
TCGAGTTTGACGTCGCCCGCATCGACGAGATCGAAGCCGTAACCAAACATGACGTCATCGCCTTTCTGACCCATCTGGCC
GAGCATGTCGGATCGGAAGAAGCGCGCTTTGTGCATCAGGGCATGACCTCTTCGGATGTGCTTGATACCTGTTTCAACGT
GCAGCTGACGCGCGCTGCGGACCTGTTGATGGTCGGCATGGACAAGCTCCTGGACGCGCTCAAAAAACGCGCGCTGGAAC
ACAAAGACACCGTGCGCGTTGGCCGCAGCCACGGCATCCACGCCGAGCCCACCACCATGGGCCTGACCTTTGCGCGCTTC
TACGCCGAGATGGACCGCAACAAGAAACGCCTGATCGCCGCACGCGAGGAAATCGCCACCGGTGCGATCTCTGGCGCCGT
GGGCACCTTTGCCAATATCGACCCACGCGTCGAAGAACATGTCTGCGAACAGCTGGGCCTCAACCCGGAGCCGATTTCCA
CGCAGGTGATCCCCCGCGACCGCCACGCCATGTTCTTTGCCACCCTCGGCGTGATTGCCTCCTCTATCGAGAACATCGCG
GTTGAGATCCGCCACATGCAGCGCACCGAGGTGCTGGAAGGTGCGGAATTCTTCTCCATGGGCCAGAAAGGCTCCTCGGC
GATGCCGCACAAGAAAAACCCGGTCCTGACCGAGAATCTCACCGGTCTTGCGCGTCTGGTGCGCATGTCGGTGATCCCGG
CGATGGAGAACGTGGCGCTTTGGCACGAGCGTGACATCTCGCACTCCTCGGTGGAGCGTGGCATCGGCCCCGACGCCACC
GTGACGCTCGATTTTGCGCTGCACCGTCTGGCGGGCGTGATCGACAAGATGCTGGTGTTCCCGGAAAACATGCTTGAGAA
CATGAACAAGTTCCCCGGCCTCGTGATGTCCCAGCGCGTTCTGCTGGCGCTCACCCAGGCGGGTGTGAGCCGCGAGGACG
CCTATTCCATGGTCCAGCGCAACGCGCTCAAGGTCTGGGAAGAGCGCCTTGATTTCCGCGAGCTGCTGCTGGCGGATGCC
GAGGTGGTTGCGGCCCTGGGCGAGGACGGCATCAACGAGAAATTCGACATGGGCTATCACACCAAACATGTCGACACGAT
CTTCAAGCGGGTGTTTGGCGAATAA

Upstream 100 bases:

>100_bases
GGCAGATCGGGCCCTGCTGCGGCGCGGCGCTTGCCGCCGCGGCATCGCTCGTCTATGAGAGCGCCATCTCTTCCACCGTC
TTTGAAAGGTGCTGCTGCCC

Downstream 100 bases:

>100_bases
TCCCACCCTCATGCACAGATCAGAAACCCCGCCCTCCTCGGCGGGGTTTTTCTTTTACCCCCTCACAGCTTCGTGAAAAC
AAAATTTGGACGATCCGCGC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MIPRYARPDMVAVWSPETKFRIWYEIEAHACDAMANLGVIPRENAEAVWKAKDVEFDVARIDEIEAVTKHDVIAFLTHLA
EHVGSEEARFVHQGMTSSDVLDTCFNVQLTRAADLLMVGMDKLLDALKKRALEHKDTVRVGRSHGIHAEPTTMGLTFARF
YAEMDRNKKRLIAAREEIATGAISGAVGTFANIDPRVEEHVCEQLGLNPEPISTQVIPRDRHAMFFATLGVIASSIENIA
VEIRHMQRTEVLEGAEFFSMGQKGSSAMPHKKNPVLTENLTGLARLVRMSVIPAMENVALWHERDISHSSVERGIGPDAT
VTLDFALHRLAGVIDKMLVFPENMLENMNKFPGLVMSQRVLLALTQAGVSREDAYSMVQRNALKVWEERLDFRELLLADA
EVVAALGEDGINEKFDMGYHTKHVDTIFKRVFGE

Sequences:

>Translated_434_residues
MIPRYARPDMVAVWSPETKFRIWYEIEAHACDAMANLGVIPRENAEAVWKAKDVEFDVARIDEIEAVTKHDVIAFLTHLA
EHVGSEEARFVHQGMTSSDVLDTCFNVQLTRAADLLMVGMDKLLDALKKRALEHKDTVRVGRSHGIHAEPTTMGLTFARF
YAEMDRNKKRLIAAREEIATGAISGAVGTFANIDPRVEEHVCEQLGLNPEPISTQVIPRDRHAMFFATLGVIASSIENIA
VEIRHMQRTEVLEGAEFFSMGQKGSSAMPHKKNPVLTENLTGLARLVRMSVIPAMENVALWHERDISHSSVERGIGPDAT
VTLDFALHRLAGVIDKMLVFPENMLENMNKFPGLVMSQRVLLALTQAGVSREDAYSMVQRNALKVWEERLDFRELLLADA
EVVAALGEDGINEKFDMGYHTKHVDTIFKRVFGE
>Mature_434_residues
MIPRYARPDMVAVWSPETKFRIWYEIEAHACDAMANLGVIPRENAEAVWKAKDVEFDVARIDEIEAVTKHDVIAFLTHLA
EHVGSEEARFVHQGMTSSDVLDTCFNVQLTRAADLLMVGMDKLLDALKKRALEHKDTVRVGRSHGIHAEPTTMGLTFARF
YAEMDRNKKRLIAAREEIATGAISGAVGTFANIDPRVEEHVCEQLGLNPEPISTQVIPRDRHAMFFATLGVIASSIENIA
VEIRHMQRTEVLEGAEFFSMGQKGSSAMPHKKNPVLTENLTGLARLVRMSVIPAMENVALWHERDISHSSVERGIGPDAT
VTLDFALHRLAGVIDKMLVFPENMLENMNKFPGLVMSQRVLLALTQAGVSREDAYSMVQRNALKVWEERLDFRELLLADA
EVVAALGEDGINEKFDMGYHTKHVDTIFKRVFGE

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=390, Percent_Identity=26.4102564102564, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI183227688, Length=383, Percent_Identity=26.6318537859008, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI1787376, Length=348, Percent_Identity=28.735632183908, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17508577, Length=416, Percent_Identity=25.9615384615385, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI32564234, Length=362, Percent_Identity=26.5193370165746, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323391, Length=391, Percent_Identity=26.0869565217391, Blast_Score=119, Evalue=9e-28,
Organism=Drosophila melanogaster, GI24647570, Length=383, Percent_Identity=25.3263707571802, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48596; Mature: 48596

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPRYARPDMVAVWSPETKFRIWYEIEAHACDAMANLGVIPRENAEAVWKAKDVEFDVAR
CCCCCCCCCEEEEECCCCCEEEEEEEEHHHHHHHHHCCCCCCCCCHHHHHHCCCCCHHHH
IDEIEAVTKHDVIAFLTHLAEHVGSEEARFVHQGMTSSDVLDTCFNVQLTRAADLLMVGM
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHH
DKLLDALKKRALEHKDTVRVGRSHGIHAEPTTMGLTFARFYAEMDRNKKRLIAAREEIAT
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
GAISGAVGTFANIDPRVEEHVCEQLGLNPEPISTQVIPRDRHAMFFATLGVIASSIENIA
HHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHH
VEIRHMQRTEVLEGAEFFSMGQKGSSAMPHKKNPVLTENLTGLARLVRMSVIPAMENVAL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
WHERDISHSSVERGIGPDATVTLDFALHRLAGVIDKMLVFPENMLENMNKFPGLVMSQRV
HHHCCCCHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHH
LLALTQAGVSREDAYSMVQRNALKVWEERLDFRELLLADAEVVAALGEDGINEKFDMGYH
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
TKHVDTIFKRVFGE
HHHHHHHHHHHHCC
>Mature Secondary Structure
MIPRYARPDMVAVWSPETKFRIWYEIEAHACDAMANLGVIPRENAEAVWKAKDVEFDVAR
CCCCCCCCCEEEEECCCCCEEEEEEEEHHHHHHHHHCCCCCCCCCHHHHHHCCCCCHHHH
IDEIEAVTKHDVIAFLTHLAEHVGSEEARFVHQGMTSSDVLDTCFNVQLTRAADLLMVGM
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHH
DKLLDALKKRALEHKDTVRVGRSHGIHAEPTTMGLTFARFYAEMDRNKKRLIAAREEIAT
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
GAISGAVGTFANIDPRVEEHVCEQLGLNPEPISTQVIPRDRHAMFFATLGVIASSIENIA
HHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHH
VEIRHMQRTEVLEGAEFFSMGQKGSSAMPHKKNPVLTENLTGLARLVRMSVIPAMENVAL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
WHERDISHSSVERGIGPDATVTLDFALHRLAGVIDKMLVFPENMLENMNKFPGLVMSQRV
HHHCCCCHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHH
LLALTQAGVSREDAYSMVQRNALKVWEERLDFRELLLADAEVVAALGEDGINEKFDMGYH
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
TKHVDTIFKRVFGE
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]