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The map label for this gene is dusA [H]

Identifier: 99080805

GI number: 99080805

Start: 1031901

End: 1032911

Strand: Direct

Name: dusA [H]

Synonym: TM1040_0964

Alternate gene names: 99080805

Gene position: 1031901-1032911 (Clockwise)

Preceding gene: 99080804

Following gene: 99080806

Centisome position: 32.24

GC content: 63.5

Gene sequence:

>1011_bases
ATGAGCACTGGTACTCTTTCAAAAGCAGACCTTTGGCGCGCGGCACGGTTGTCGGTTGCGCCGATGATGGATTGGACCAC
ACGGGAGTGCCGCTATCTGCATCGGCTGCTCTCGCGTGAGACCTTGCTCTACACCGAAATGGTGACGGCGCCGGCACTGG
TGCGGGGGGGGGCTTTGCATCTGCTCGATCACGACCCAAGCGAACATCCGGTTGCGTTGCAGCTTGGCGGCTCCGACCCC
AAGGAACTGGCAGAGGCCGCGCGGCTCGGGGCCGAGGCGGGCTATGATGAGATCAACCTCAACTGCGGCTGCCCGAGCGA
CCGGGTGCAATCTGGCGCTTTTGGGGCTGTCTTGATGCAGACGCCCGAACTGGTGGCCGACTGTGTGGCCGCGATGGCCG
AGGCGGTCTCTGTCGAGGTGACCGTGAAATGCCGCATCGGCGTGGACGATCAAACCCCAGATGAGATCTTGCCAGACTTT
CTCGACAAGATCCGCGCGGCGGGGGTCAAGCGGGTGACAATTCATGCCCGCAAAGCCTGGCTGCAAGGGCTTTCACCCAA
GGAAAATCGCGACATCCCGCCGCTGGATTACGAGCTTGTGCACGCGATGAAGTCGGCCTTTGCCGATATGCATATCTCGC
TCAATGGCGGCATCACCTCGCTTGATGAAGCCCAGGCGCAGTTAGCACGCGGGCTGGATGGGGTTATGGTTGGACGTGCC
GCCTATCACCAGCCCGCCGATGTGCTCTGTGCTGCAGATCCGCGGATCTACGGCACCGGGACGGCGAGCGATCCGCTCGA
GGTGGTGCGCCAGATGCTGCCCTATATCGAACAGCAGATGCAATCGGGATCGCGGCTTCATCAGATCACGCGCCATATGC
TTGGGCTTTTTGCCGGTCGCCCGGGGGCGCGGCAGTGGCGGCGTCACCTCTCCGAGGGCGCACACCGCACCGGCGCGGGC
CCAGAGCTTGTCGAAGAGGCCCTCTCCCTCGTGTTGGCGCGGGCGGCCTGA

Upstream 100 bases:

>100_bases
TCTCCCGATACTGCACCAGAGAGCCGCGTGCCTCGCGGCATTTGGCGTTCATAGCGAGATGCGCGGCAATCACACGGCTA
GAGGCGCTAGGAAAACCTGG

Downstream 100 bases:

>100_bases
GGCAGATGACGGCAACCGAGGCTTACCTGCAAAAGGGCTGGCTGCGTTTTGCAGCAGAAGAGAGCATCAGAGATTGGGCC
GCGCACGCGCAGCGCTGTGC

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MSTGTLSKADLWRAARLSVAPMMDWTTRECRYLHRLLSRETLLYTEMVTAPALVRGGALHLLDHDPSEHPVALQLGGSDP
KELAEAARLGAEAGYDEINLNCGCPSDRVQSGAFGAVLMQTPELVADCVAAMAEAVSVEVTVKCRIGVDDQTPDEILPDF
LDKIRAAGVKRVTIHARKAWLQGLSPKENRDIPPLDYELVHAMKSAFADMHISLNGGITSLDEAQAQLARGLDGVMVGRA
AYHQPADVLCAADPRIYGTGTASDPLEVVRQMLPYIEQQMQSGSRLHQITRHMLGLFAGRPGARQWRRHLSEGAHRTGAG
PELVEEALSLVLARAA

Sequences:

>Translated_336_residues
MSTGTLSKADLWRAARLSVAPMMDWTTRECRYLHRLLSRETLLYTEMVTAPALVRGGALHLLDHDPSEHPVALQLGGSDP
KELAEAARLGAEAGYDEINLNCGCPSDRVQSGAFGAVLMQTPELVADCVAAMAEAVSVEVTVKCRIGVDDQTPDEILPDF
LDKIRAAGVKRVTIHARKAWLQGLSPKENRDIPPLDYELVHAMKSAFADMHISLNGGITSLDEAQAQLARGLDGVMVGRA
AYHQPADVLCAADPRIYGTGTASDPLEVVRQMLPYIEQQMQSGSRLHQITRHMLGLFAGRPGARQWRRHLSEGAHRTGAG
PELVEEALSLVLARAA
>Mature_335_residues
STGTLSKADLWRAARLSVAPMMDWTTRECRYLHRLLSRETLLYTEMVTAPALVRGGALHLLDHDPSEHPVALQLGGSDPK
ELAEAARLGAEAGYDEINLNCGCPSDRVQSGAFGAVLMQTPELVADCVAAMAEAVSVEVTVKCRIGVDDQTPDEILPDFL
DKIRAAGVKRVTIHARKAWLQGLSPKENRDIPPLDYELVHAMKSAFADMHISLNGGITSLDEAQAQLARGLDGVMVGRAA
YHQPADVLCAADPRIYGTGTASDPLEVVRQMLPYIEQQMQSGSRLHQITRHMLGLFAGRPGARQWRRHLSEGAHRTGAGP
ELVEEALSLVLARAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=235, Percent_Identity=30.6382978723404, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI239788483, Length=252, Percent_Identity=25.7936507936508, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI40807366, Length=283, Percent_Identity=28.6219081272085, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI239788462, Length=252, Percent_Identity=25.7936507936508, Blast_Score=75, Evalue=7e-14,
Organism=Escherichia coli, GI145693211, Length=326, Percent_Identity=58.2822085889571, Blast_Score=372, Evalue=1e-104,
Organism=Escherichia coli, GI1789660, Length=236, Percent_Identity=30.5084745762712, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788462, Length=231, Percent_Identity=29.8701298701299, Blast_Score=82, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI25144369, Length=234, Percent_Identity=29.0598290598291, Blast_Score=78, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6323560, Length=167, Percent_Identity=31.1377245508982, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24580595, Length=241, Percent_Identity=28.6307053941909, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19920448, Length=241, Percent_Identity=28.6307053941909, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 36450; Mature: 36319

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTGTLSKADLWRAARLSVAPMMDWTTRECRYLHRLLSRETLLYTEMVTAPALVRGGALH
CCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
LLDHDPSEHPVALQLGGSDPKELAEAARLGAEAGYDEINLNCGCPSDRVQSGAFGAVLMQ
EECCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHCCCCCHHHHHC
TPELVADCVAAMAEAVSVEVTVKCRIGVDDQTPDEILPDFLDKIRAAGVKRVTIHARKAW
CHHHHHHHHHHHHHHHEEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEHHHHHHH
LQGLSPKENRDIPPLDYELVHAMKSAFADMHISLNGGITSLDEAQAQLARGLDGVMVGRA
HCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHCCCCHHHCCHH
AYHQPADVLCAADPRIYGTGTASDPLEVVRQMLPYIEQQMQSGSRLHQITRHMLGLFAGR
HHCCCCCEEEECCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCC
PGARQWRRHLSEGAHRTGAGPELVEEALSLVLARAA
CCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STGTLSKADLWRAARLSVAPMMDWTTRECRYLHRLLSRETLLYTEMVTAPALVRGGALH
CCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
LLDHDPSEHPVALQLGGSDPKELAEAARLGAEAGYDEINLNCGCPSDRVQSGAFGAVLMQ
EECCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHCCCCCHHHHHC
TPELVADCVAAMAEAVSVEVTVKCRIGVDDQTPDEILPDFLDKIRAAGVKRVTIHARKAW
CHHHHHHHHHHHHHHHEEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEHHHHHHH
LQGLSPKENRDIPPLDYELVHAMKSAFADMHISLNGGITSLDEAQAQLARGLDGVMVGRA
HCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHCCCCHHHCCHH
AYHQPADVLCAADPRIYGTGTASDPLEVVRQMLPYIEQQMQSGSRLHQITRHMLGLFAGR
HHCCCCCEEEECCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCC
PGARQWRRHLSEGAHRTGAGPELVEEALSLVLARAA
CCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]