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The map label for this gene is ydgK [H]

Identifier: 99080797

GI number: 99080797

Start: 1021740

End: 1022948

Strand: Direct

Name: ydgK [H]

Synonym: TM1040_0956

Alternate gene names: 99080797

Gene position: 1021740-1022948 (Clockwise)

Preceding gene: 99080796

Following gene: 99080798

Centisome position: 31.92

GC content: 63.52

Gene sequence:

>1209_bases
ATGCTGCGCCCAGCTCAGACCCCGCCGATGTTGATGACATTGGTCTTTGGCACCGCGTTGTCGGTGCTCTCGCTCAATAT
GTTCCTGCCCTCTCTGCCCGGCATCGCACAGGAGTTCGGCGTGGACTACGGGCTCGCGAACCTTGCGATTGCCGGCTATC
TGACGATTTCCGCGGGGCTGCAGCTCATTATGGGGCCGATGTCCGATCTCTACGGGCGCCGGAGGGTGATGCTCCTGTGC
TGCGCGCTCTTTGTGCTCGCCTCCGTGGGCTGCGCGCTGGCCAGCAACATCTGGGTGTTTCTCGGCTTTCGCTTGCTGCA
GGGCGCGATCATCAGCGGGCAGGTGATTGCACGTGCCGTGATCCGCGACATGTACCCACCAAGCGAGGCGGCCAGCAAAC
TCGGCTACATGAGCATGATCATGGCGCTGGCGCCGATGACAGGGCCGATGATTGGGGGCGCGCTTGATATGGCGTTTGGC
TGGCGCGCAGGGTTCTGGCTCTATTCCGGGCTTGGGTTGCTGGTGCTCTGGCTCACCTGGGGCGACATGGGAGAGACCAA
CACCAAGCGCGCGGCGACCTTTCGCGCGCAGATGCGGCTTTATCCGCGTTTGTTCAGCTCGCGCCGCTTCTGGGGGTATT
CGGCCTGCGTCGCCTTCTCTATCGGCGGGTTCTATTCCTTTGTGACCGGGGCGCCGATGGTCGCGGCGGCGTGGTTTGAT
CTGAGCCCGGCCATGGTGGGCCTTGGGATCGGGATTATCACCTGCGGCTTCATGGTTGGCAATTTTGTCACCGGCCAGCT
GGCACAGCGCCTGCGTTTGCTGACGCTTATCCTGATCGGCCGGGGGGTGGCCTGTCTTGGGCCGGCGGTGGGTCTTGCGC
TGTTCTTCATGGGGCAGGGGAGCGAACTGGTGTTCTTTGGGTCCGCCATCTGCGTGGGCTTTGGCAACGGGCTGACGATC
GCAAATGCCAGTGCCGGGCTGATGTCGGTACGCCCCGAGCTTGCCGGCAGTGCCGCAGGGCTTTCGGGGGCGCTAAGCGT
GGCGCTGGGCGCACTCCTCACGGCTGTCACCGGTGTGGCCGTTGAACCCGAAACCGCGCCGTTTGCAGTGCTCGGCATCA
TGTTGAGCGCAAGTGTGCTGGCGCTTGTGTCGGCACTCTATGTGTTGGTTGTAGACCTGCAGGATCCGCTGGAACCCGCG
CATGGGTGA

Upstream 100 bases:

>100_bases
GCCCCTTGTGTCTGACCCGAGGGCGTTGTTTAACACATGCAGTACTCAAGGCTTGCGCTACGTGCGGATGCCGCAGAGGG
CCAAGAGAACGGAGTGTCCC

Downstream 100 bases:

>100_bases
TCTTCATCGGGCTTTCGGTGCTGCAGTGCGCACAAGGCTCTTCCCAAGGGCGGGCTGTCGTGCTAGGCGGCGCCAGATCG
TTGCGGCACCCTGGTTTCGG

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MLRPAQTPPMLMTLVFGTALSVLSLNMFLPSLPGIAQEFGVDYGLANLAIAGYLTISAGLQLIMGPMSDLYGRRRVMLLC
CALFVLASVGCALASNIWVFLGFRLLQGAIISGQVIARAVIRDMYPPSEAASKLGYMSMIMALAPMTGPMIGGALDMAFG
WRAGFWLYSGLGLLVLWLTWGDMGETNTKRAATFRAQMRLYPRLFSSRRFWGYSACVAFSIGGFYSFVTGAPMVAAAWFD
LSPAMVGLGIGIITCGFMVGNFVTGQLAQRLRLLTLILIGRGVACLGPAVGLALFFMGQGSELVFFGSAICVGFGNGLTI
ANASAGLMSVRPELAGSAAGLSGALSVALGALLTAVTGVAVEPETAPFAVLGIMLSASVLALVSALYVLVVDLQDPLEPA
HG

Sequences:

>Translated_402_residues
MLRPAQTPPMLMTLVFGTALSVLSLNMFLPSLPGIAQEFGVDYGLANLAIAGYLTISAGLQLIMGPMSDLYGRRRVMLLC
CALFVLASVGCALASNIWVFLGFRLLQGAIISGQVIARAVIRDMYPPSEAASKLGYMSMIMALAPMTGPMIGGALDMAFG
WRAGFWLYSGLGLLVLWLTWGDMGETNTKRAATFRAQMRLYPRLFSSRRFWGYSACVAFSIGGFYSFVTGAPMVAAAWFD
LSPAMVGLGIGIITCGFMVGNFVTGQLAQRLRLLTLILIGRGVACLGPAVGLALFFMGQGSELVFFGSAICVGFGNGLTI
ANASAGLMSVRPELAGSAAGLSGALSVALGALLTAVTGVAVEPETAPFAVLGIMLSASVLALVSALYVLVVDLQDPLEPA
HG
>Mature_402_residues
MLRPAQTPPMLMTLVFGTALSVLSLNMFLPSLPGIAQEFGVDYGLANLAIAGYLTISAGLQLIMGPMSDLYGRRRVMLLC
CALFVLASVGCALASNIWVFLGFRLLQGAIISGQVIARAVIRDMYPPSEAASKLGYMSMIMALAPMTGPMIGGALDMAFG
WRAGFWLYSGLGLLVLWLTWGDMGETNTKRAATFRAQMRLYPRLFSSRRFWGYSACVAFSIGGFYSFVTGAPMVAAAWFD
LSPAMVGLGIGIITCGFMVGNFVTGQLAQRLRLLTLILIGRGVACLGPAVGLALFFMGQGSELVFFGSAICVGFGNGLTI
ANASAGLMSVRPELAGSAAGLSGALSVALGALLTAVTGVAVEPETAPFAVLGIMLSASVLALVSALYVLVVDLQDPLEPA
HG

Specific function: Involved In Sulfonamide (Sulfathiazole) And Bicyclomycin Resistance. Probable Membrane Translocase. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=349, Percent_Identity=26.647564469914, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI48994889, Length=376, Percent_Identity=25.7978723404255, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1790146, Length=374, Percent_Identity=25.4010695187166, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1790794, Length=235, Percent_Identity=25.531914893617, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787065, Length=340, Percent_Identity=24.7058823529412, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87082312, Length=141, Percent_Identity=29.0780141843972, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324264, Length=186, Percent_Identity=27.4193548387097, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322071, Length=168, Percent_Identity=28.5714285714286, Blast_Score=64, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6319517, Length=178, Percent_Identity=26.9662921348315, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42047; Mature: 42047

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure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ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 9202461 [H]