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The map label for this gene is ksgA

Identifier: 99080785

GI number: 99080785

Start: 1006604

End: 1007446

Strand: Reverse

Name: ksgA

Synonym: TM1040_0944

Alternate gene names: 99080785

Gene position: 1007446-1006604 (Counterclockwise)

Preceding gene: 99080786

Following gene: 99080781

Centisome position: 31.47

GC content: 65.6

Gene sequence:

>843_bases
ATGAGCGCTATCGACAATCTCCCCCCGCTGCGCGAGGTCATCGCGACCCACCAGCTCTCGGCCCGCAAATCGCTGGGTCA
GAACTTTCTTCTTGATCTGAACCTGACCGCCAAGATCGCGCGTCAGGCGGGTGATCTCACAGGCTGCGACGTCTTGGAGA
TCGGCCCTGGCCCCGGCGGCCTCACACGCGGGCTTCTCAGCGAAGGCGCGCGCAAGATCCTCGCCATCGAAAAAGACCAG
CGCTGCCTGCCCGCCCTGGAGGACATCGCCGCCGCCTATCCGGGGCGCCTCGAGGTCATCAACGGGGACGCGCTGGAGAT
TGACCCGCTGGCGCATCTGACGCCGCCGATCCGCGTCGCCGCCAACCTGCCTTACAATGTCGGCACCGAACTTCTGGTGC
GGTGGCTCACCCCAAAAGAGTGGCCCCCGTTCTGGCAGAGCCTCACTTTGATGTTCCAGCGCGAAGTGGCCGAGCGCATC
GTTGCGCAACCTGGCTCCAAAGCCTACGGGCGGCTGGCAATCCTTGCGCAGTGGCGTGCCGAGGCCAGGATCGCCCTCTC
GCTGCCCCCAGGCGCCTTTACGCCACCGCCAAAGGTCTCGAGCGCCGTGGTCCATCTGACGGCCCTGCCAGAGCCCCGCT
ACCCAGCCGATGCCGCGATCCTGAGCCGCGTGGTTGCCGCCGCTTTCAATCAGCGTCGCAAGATGCTGCGCGCCTCTCTC
AAAGGCGTCTCTCCCCAGATCGAAGACCACTTGAACGCCGCGGGCATTCCGCCAACCGAACGCGCCGAGCAGGTCAGCGT
TGAGGACTTCTGCGCCCTTGCCCGTAGTTTGGAGAAGGGCTGA

Upstream 100 bases:

>100_bases
CTCATCCCAAGTCAAACCTGCGCGCAGGGAAGCCCCTGCGCACCTTTCGGATGCAAATCCCGCTTTCCAGATGCCCGCGC
GCGCCTTATGAAGCTCCCTC

Downstream 100 bases:

>100_bases
TCCAGCGCACTCTTACGGCCCGCCCCGACCATTGCGCCGGACATGAAAAAACCCCCGCAGCGCCTGCTGTGGGGGTTTTG
TCTGATCTGGATTGATCCCG

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MSAIDNLPPLREVIATHQLSARKSLGQNFLLDLNLTAKIARQAGDLTGCDVLEIGPGPGGLTRGLLSEGARKILAIEKDQ
RCLPALEDIAAAYPGRLEVINGDALEIDPLAHLTPPIRVAANLPYNVGTELLVRWLTPKEWPPFWQSLTLMFQREVAERI
VAQPGSKAYGRLAILAQWRAEARIALSLPPGAFTPPPKVSSAVVHLTALPEPRYPADAAILSRVVAAAFNQRRKMLRASL
KGVSPQIEDHLNAAGIPPTERAEQVSVEDFCALARSLEKG

Sequences:

>Translated_280_residues
MSAIDNLPPLREVIATHQLSARKSLGQNFLLDLNLTAKIARQAGDLTGCDVLEIGPGPGGLTRGLLSEGARKILAIEKDQ
RCLPALEDIAAAYPGRLEVINGDALEIDPLAHLTPPIRVAANLPYNVGTELLVRWLTPKEWPPFWQSLTLMFQREVAERI
VAQPGSKAYGRLAILAQWRAEARIALSLPPGAFTPPPKVSSAVVHLTALPEPRYPADAAILSRVVAAAFNQRRKMLRASL
KGVSPQIEDHLNAAGIPPTERAEQVSVEDFCALARSLEKG
>Mature_279_residues
SAIDNLPPLREVIATHQLSARKSLGQNFLLDLNLTAKIARQAGDLTGCDVLEIGPGPGGLTRGLLSEGARKILAIEKDQR
CLPALEDIAAAYPGRLEVINGDALEIDPLAHLTPPIRVAANLPYNVGTELLVRWLTPKEWPPFWQSLTLMFQREVAERIV
AQPGSKAYGRLAILAQWRAEARIALSLPPGAFTPPPKVSSAVVHLTALPEPRYPADAAILSRVVAAAFNQRRKMLRASLK
GVSPQIEDHLNAAGIPPTERAEQVSVEDFCALARSLEKG

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=295, Percent_Identity=37.2881355932203, Blast_Score=195, Evalue=4e-50,
Organism=Homo sapiens, GI7657198, Length=201, Percent_Identity=35.8208955223881, Blast_Score=101, Evalue=7e-22,
Organism=Escherichia coli, GI1786236, Length=264, Percent_Identity=38.2575757575758, Blast_Score=150, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI25141369, Length=295, Percent_Identity=31.5254237288136, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI25146882, Length=285, Percent_Identity=28.4210526315789, Blast_Score=97, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6324989, Length=242, Percent_Identity=29.3388429752066, Blast_Score=82, Evalue=8e-17,
Organism=Drosophila melanogaster, GI21357273, Length=286, Percent_Identity=33.2167832167832, Blast_Score=141, Evalue=4e-34,
Organism=Drosophila melanogaster, GI21358017, Length=229, Percent_Identity=35.8078602620087, Blast_Score=105, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_SILST (Q1GI39)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_612939.1
- ProteinModelPortal:   Q1GI39
- SMR:   Q1GI39
- STRING:   Q1GI39
- GeneID:   4077338
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_0944
- NMPDR:   fig|292414.1.peg.3024
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   GVLCGWR
- PhylomeDB:   Q1GI39
- ProtClustDB:   PRK00274
- BioCyc:   SSP292414:TM1040_0944-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 30279; Mature: 30148

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 28-28 BINDING 30-30 BINDING 55-55 BINDING 77-77 BINDING 103-103 BINDING 122-122

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAIDNLPPLREVIATHQLSARKSLGQNFLLDLNLTAKIARQAGDLTGCDVLEIGPGPGG
CCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHCCCCCCCCEEEECCCCCH
LTRGLLSEGARKILAIEKDQRCLPALEDIAAAYPGRLEVINGDALEIDPLAHLTPPIRVA
HHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCEEECCHHHCCCCEEEE
ANLPYNVGTELLVRWLTPKEWPPFWQSLTLMFQREVAERIVAQPGSKAYGRLAILAQWRA
ECCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
EARIALSLPPGAFTPPPKVSSAVVHLTALPEPRYPADAAILSRVVAAAFNQRRKMLRASL
CCEEEEECCCCCCCCCCCCCHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KGVSPQIEDHLNAAGIPPTERAEQVSVEDFCALARSLEKG
HCCCCHHHHHCCCCCCCCCCHHHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SAIDNLPPLREVIATHQLSARKSLGQNFLLDLNLTAKIARQAGDLTGCDVLEIGPGPGG
CCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHCCCCCCCCEEEECCCCCH
LTRGLLSEGARKILAIEKDQRCLPALEDIAAAYPGRLEVINGDALEIDPLAHLTPPIRVA
HHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCEEECCHHHCCCCEEEE
ANLPYNVGTELLVRWLTPKEWPPFWQSLTLMFQREVAERIVAQPGSKAYGRLAILAQWRA
ECCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
EARIALSLPPGAFTPPPKVSSAVVHLTALPEPRYPADAAILSRVVAAAFNQRRKMLRASL
CCEEEEECCCCCCCCCCCCCHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KGVSPQIEDHLNAAGIPPTERAEQVSVEDFCALARSLEKG
HCCCCHHHHHCCCCCCCCCCHHHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA