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The map label for this gene is ispG

Identifier: 99080703

GI number: 99080703

Start: 915958

End: 917091

Strand: Direct

Name: ispG

Synonym: TM1040_0862

Alternate gene names: 99080703

Gene position: 915958-917091 (Clockwise)

Preceding gene: 99080702

Following gene: 99080706

Centisome position: 28.62

GC content: 60.05

Gene sequence:

>1134_bases
ATGTCCATGAATCATATCCGCCCTTGGCGGAACATCGAACGCCGCAAGAGCCGCCAGATCCATGTGGGCAATGTGCCCGT
AGGGGGGGATGCGCCAATTGCGGTGCAGACCATGACCAACACGTTGACCACCGACATCAAAGGCACCATCGCACAGGTGC
AGGCCGCCGCCGATGCGGGCGCCGATATAGTGCGTGTCTCGGTCCCGGACGAGGCCTCTGCCCGCGCGCTCAAGGAGATC
GTGCGGGAAAGCCCGGTGCCGATCGTGGCCGATATTCATTTCCACTACAAACGTGGCATCGAGGCCGCCGAGGCCGGTGC
CGCCTGCCTGCGCATCAATCCCGGAAACATCGGCGACGAAAAACGCGTGGCCGAGGTGATCAAGGCCGCCCGCGACCACA
ATTGCTCGATCCGTATTGGCGTCAATGCTGGCAGCCTCGAAAAACACCTCCTCGAAAAATACGGCGAGCCTTGCCCGGAC
GCGATGGTCGAATCCGGCCTTGATCACATCAAGATCCTTCAGGACCACGACTTTCATGAATTCAAGATCTCGGTCAAAGC
CTCCGACGTCTTCATGTCTGCCGCCGCTTATCAGATGCTTGCGGATGCGACCGACGCCCCCATCCACCTCGGCATCACCG
AGGCTGGTGGGCTGATGTCCGGCACGATCAAGTCTGCGATCGGCCTCGGACAGCTCTTGTGGATGGGCATTGGCGACACC
CTGCGCGTAAGCCTTTCCGCCGATCCGGTTGAAGAGGTCAAAGTAGGCTTTGAGATCCTCAAATCCCTCGGCCTGCGTCA
CCGGGGCGTCAATATCATCTCCTGCCCGAGCTGCGCGCGTCAGGGGTTTGACGTTATCAAAACGGTCGAAACCCTCGAAG
AGCGCCTCGAACACATCAAAACCCCCATGAGCCTCTCGATCATCGGCTGCGTTGTGAATGGTCCGGGCGAGGCTTTGATG
ACCGATGTCGGCTTCACCGGTGGCGGCGCGGGCTCCGGCATGGTGTATCTTGCGGGCAAGGCCAGCCACAAGATGTCCAA
CGACCAGATGATCGATCACATCGTCGAAGAGGTCGAGAAAAAAGCCGCCGCTCTCGACGCGCAAGCGGCTGAGGACATGA
AAGCGGCGGAATAA

Upstream 100 bases:

>100_bases
TCAAAACACCCATGGATTCGCAGAACGCCCCGGACAGCCATTGCAGGCTCCGGGGCGTCGGCCTATCTAAGGGGCAAGCG
ACACGCAGGACGAGGCAGCC

Downstream 100 bases:

>100_bases
CCCCGCCTTCATCTTCTTGGAAATATCCCGGGGGAGGCCCCGAAGGGGCCGGGGGCAGCGCCCCCGGTACGGGCGCTCAG
CTGCGCCCGGCGCGTATGTC

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MSMNHIRPWRNIERRKSRQIHVGNVPVGGDAPIAVQTMTNTLTTDIKGTIAQVQAAADAGADIVRVSVPDEASARALKEI
VRESPVPIVADIHFHYKRGIEAAEAGAACLRINPGNIGDEKRVAEVIKAARDHNCSIRIGVNAGSLEKHLLEKYGEPCPD
AMVESGLDHIKILQDHDFHEFKISVKASDVFMSAAAYQMLADATDAPIHLGITEAGGLMSGTIKSAIGLGQLLWMGIGDT
LRVSLSADPVEEVKVGFEILKSLGLRHRGVNIISCPSCARQGFDVIKTVETLEERLEHIKTPMSLSIIGCVVNGPGEALM
TDVGFTGGGAGSGMVYLAGKASHKMSNDQMIDHIVEEVEKKAAALDAQAAEDMKAAE

Sequences:

>Translated_377_residues
MSMNHIRPWRNIERRKSRQIHVGNVPVGGDAPIAVQTMTNTLTTDIKGTIAQVQAAADAGADIVRVSVPDEASARALKEI
VRESPVPIVADIHFHYKRGIEAAEAGAACLRINPGNIGDEKRVAEVIKAARDHNCSIRIGVNAGSLEKHLLEKYGEPCPD
AMVESGLDHIKILQDHDFHEFKISVKASDVFMSAAAYQMLADATDAPIHLGITEAGGLMSGTIKSAIGLGQLLWMGIGDT
LRVSLSADPVEEVKVGFEILKSLGLRHRGVNIISCPSCARQGFDVIKTVETLEERLEHIKTPMSLSIIGCVVNGPGEALM
TDVGFTGGGAGSGMVYLAGKASHKMSNDQMIDHIVEEVEKKAAALDAQAAEDMKAAE
>Mature_376_residues
SMNHIRPWRNIERRKSRQIHVGNVPVGGDAPIAVQTMTNTLTTDIKGTIAQVQAAADAGADIVRVSVPDEASARALKEIV
RESPVPIVADIHFHYKRGIEAAEAGAACLRINPGNIGDEKRVAEVIKAARDHNCSIRIGVNAGSLEKHLLEKYGEPCPDA
MVESGLDHIKILQDHDFHEFKISVKASDVFMSAAAYQMLADATDAPIHLGITEAGGLMSGTIKSAIGLGQLLWMGIGDTL
RVSLSADPVEEVKVGFEILKSLGLRHRGVNIISCPSCARQGFDVIKTVETLEERLEHIKTPMSLSIIGCVVNGPGEALMT
DVGFTGGGAGSGMVYLAGKASHKMSNDQMIDHIVEEVEKKAAALDAQAAEDMKAAE

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=366, Percent_Identity=58.4699453551913, Blast_Score=435, Evalue=1e-123,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_SILST (Q1GIC1)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_612857.1
- ProteinModelPortal:   Q1GIC1
- STRING:   Q1GIC1
- GeneID:   4076232
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_0862
- NMPDR:   fig|292414.1.peg.2946
- eggNOG:   COG0821
- HOGENOM:   HBG335271
- OMA:   APIVVQS
- PhylomeDB:   Q1GIC1
- ProtClustDB:   PRK00366
- BioCyc:   SSP292414:TM1040_0862-MONOMER
- HAMAP:   MF_00159
- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF004640
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE; SSF51717 DHP_synth_like

EC number: =1.17.7.1

Molecular weight: Translated: 40234; Mature: 40102

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMNHIRPWRNIERRKSRQIHVGNVPVGGDAPIAVQTMTNTLTTDIKGTIAQVQAAADAG
CCCCCCCCHHHHHHHCCCCEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCC
ADIVRVSVPDEASARALKEIVRESPVPIVADIHFHYKRGIEAAEAGAACLRINPGNIGDE
CCEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEHHHHCCCCHHHCCCEEEEECCCCCCCH
KRVAEVIKAARDHNCSIRIGVNAGSLEKHLLEKYGEPCPDAMVESGLDHIKILQDHDFHE
HHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCCHHHHHCCCCCEEEECCCCCEE
FKISVKASDVFMSAAAYQMLADATDAPIHLGITEAGGLMSGTIKSAIGLGQLLWMGIGDT
EEEEEEHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCE
LRVSLSADPVEEVKVGFEILKSLGLRHRGVNIISCPSCARQGFDVIKTVETLEERLEHIK
EEEEECCCCHHHHHHHHHHHHHHCCHHCCCCEEECCHHHHHCHHHHHHHHHHHHHHHHHC
TPMSLSIIGCVVNGPGEALMTDVGFTGGGAGSGMVYLAGKASHKMSNDQMIDHIVEEVEK
CCCEEEEEEEEECCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHH
KAAALDAQAAEDMKAAE
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SMNHIRPWRNIERRKSRQIHVGNVPVGGDAPIAVQTMTNTLTTDIKGTIAQVQAAADAG
CCCCCCCHHHHHHHCCCCEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCC
ADIVRVSVPDEASARALKEIVRESPVPIVADIHFHYKRGIEAAEAGAACLRINPGNIGDE
CCEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEHHHHCCCCHHHCCCEEEEECCCCCCCH
KRVAEVIKAARDHNCSIRIGVNAGSLEKHLLEKYGEPCPDAMVESGLDHIKILQDHDFHE
HHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCCHHHHHCCCCCEEEECCCCCEE
FKISVKASDVFMSAAAYQMLADATDAPIHLGITEAGGLMSGTIKSAIGLGQLLWMGIGDT
EEEEEEHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCE
LRVSLSADPVEEVKVGFEILKSLGLRHRGVNIISCPSCARQGFDVIKTVETLEERLEHIK
EEEEECCCCHHHHHHHHHHHHHHCCHHCCCCEEECCHHHHHCHHHHHHHHHHHHHHHHHC
TPMSLSIIGCVVNGPGEALMTDVGFTGGGAGSGMVYLAGKASHKMSNDQMIDHIVEEVEK
CCCEEEEEEEEECCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHH
KAAALDAQAAEDMKAAE
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA