Click here to switch to the map view.

The map label for this gene is gcvA [H]

Identifier: 99080685

GI number: 99080685

Start: 894888

End: 895775

Strand: Reverse

Name: gcvA [H]

Synonym: TM1040_0844

Alternate gene names: 99080685

Gene position: 895775-894888 (Counterclockwise)

Preceding gene: 99080689

Following gene: 99080681

Centisome position: 27.98

GC content: 62.95

Gene sequence:

>888_bases
ATGTATCAGGAATTGCCCCCTCTGCCATGGCTGCGCGCTTTTGATGCCAGCGCCCGGCTTGGAAACTTCACCCTCGCCGC
CCAGGAGCTTGGGCTGACGCCCTCTGCAGTCAGCTACCAGGTGCGTGGACTTGAGGCGACGCTCGGCCACAAGCTCTTTG
TGAGAAAGCAAAAATCGCTATTCCTGACACGGCTGGGACAGGCTTACCTGCCCGTGGTGAGCAAGGCATTTGGCGATCTG
GAGGCCACCACGTCGAACCTCTTTGGCAAGAGTGCTGCGCAAGAAATCACGCTCAGGTGCCTGAGTTCGCTCAACATGCT
GTGGCTTGCGCCGCGGCTTTCGCGTTTTCAGGCGCAGCACCCGGACTGCCGCCTGCGGGTGCTCTCGGCGCTTTGGGGTG
AGACCCCAGAGGCGCTGCAGATCGACATCGACATTCGCTATGGCAATGGCAGTTGGTCAGATGGCGAGGTGACGCGTCTG
ATGCATCACGAGGTGCTCGCTGTCTGTGCGCCTGCGCTGCATCCCGGCCCCGATCCGCAGGCGATTGCGTCCTGCCCGCT
GATTGAGGTGATTGGCGTCACCGACACATGGCATCACTTCTTTGCCCTGCATGGGCTCGCACCTCCCTCTTCTGGGCCGG
CGTTGAAGGTCGACCAATCGCTGATCGCGCTTGAGCTCGCCACGCGTGGCATGGGCGTTGCGCTGATCGCCCGTGTCTTT
GCCCAGCGCTATCTGGAGAGCGGTGCCTTGGTGGAGGCGACGGACCTCGCCCTGCCCGCGCGCGAGGGGCACTACATCGT
GCAGCCGGAGGCCCGCAATCACTTTCGCCCGGAAGTCGCGGCGCTCAAAGACTGGCTCTTGCAGGAGGCCGCGCAAACAG
GCTGTTGA

Upstream 100 bases:

>100_bases
ATTCATCGCGCCAGCGTTACAGCTCCGGCTCCAATCCCCATAACGAAAAATAATTCGCCGTAACCTTAAAAATATTCGCA
GATTGGTGTAGAGCAAAACC

Downstream 100 bases:

>100_bases
TCTCCCCCGGCGCAGAGGCGCACCGGGAGAGATCTGGCTTCATCAATTTGTCGCAACACTCAGCGGATAGTCCACGTTGA
GCGAAAGCGCCGGGATCTGT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MYQELPPLPWLRAFDASARLGNFTLAAQELGLTPSAVSYQVRGLEATLGHKLFVRKQKSLFLTRLGQAYLPVVSKAFGDL
EATTSNLFGKSAAQEITLRCLSSLNMLWLAPRLSRFQAQHPDCRLRVLSALWGETPEALQIDIDIRYGNGSWSDGEVTRL
MHHEVLAVCAPALHPGPDPQAIASCPLIEVIGVTDTWHHFFALHGLAPPSSGPALKVDQSLIALELATRGMGVALIARVF
AQRYLESGALVEATDLALPAREGHYIVQPEARNHFRPEVAALKDWLLQEAAQTGC

Sequences:

>Translated_295_residues
MYQELPPLPWLRAFDASARLGNFTLAAQELGLTPSAVSYQVRGLEATLGHKLFVRKQKSLFLTRLGQAYLPVVSKAFGDL
EATTSNLFGKSAAQEITLRCLSSLNMLWLAPRLSRFQAQHPDCRLRVLSALWGETPEALQIDIDIRYGNGSWSDGEVTRL
MHHEVLAVCAPALHPGPDPQAIASCPLIEVIGVTDTWHHFFALHGLAPPSSGPALKVDQSLIALELATRGMGVALIARVF
AQRYLESGALVEATDLALPAREGHYIVQPEARNHFRPEVAALKDWLLQEAAQTGC
>Mature_295_residues
MYQELPPLPWLRAFDASARLGNFTLAAQELGLTPSAVSYQVRGLEATLGHKLFVRKQKSLFLTRLGQAYLPVVSKAFGDL
EATTSNLFGKSAAQEITLRCLSSLNMLWLAPRLSRFQAQHPDCRLRVLSALWGETPEALQIDIDIRYGNGSWSDGEVTRL
MHHEVLAVCAPALHPGPDPQAIASCPLIEVIGVTDTWHHFFALHGLAPPSSGPALKVDQSLIALELATRGMGVALIARVF
AQRYLESGALVEATDLALPAREGHYIVQPEARNHFRPEVAALKDWLLQEAAQTGC

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=295, Percent_Identity=34.9152542372881, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI1786448, Length=288, Percent_Identity=32.2916666666667, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1788706, Length=295, Percent_Identity=27.1186440677966, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1786508, Length=134, Percent_Identity=30.5970149253731, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI1790262, Length=258, Percent_Identity=27.1317829457364, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI87082024, Length=271, Percent_Identity=29.1512915129151, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32274; Mature: 32274

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQELPPLPWLRAFDASARLGNFTLAAQELGLTPSAVSYQVRGLEATLGHKLFVRKQKSL
CCCCCCCCCHHHHCCCCCCCCCEEEEHHHHCCCCCHHEEEEECHHHHHCCHHHHHHHHHH
FLTRLGQAYLPVVSKAFGDLEATTSNLFGKSAAQEITLRCLSSLNMLWLAPRLSRFQAQH
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCC
PDCRLRVLSALWGETPEALQIDIDIRYGNGSWSDGEVTRLMHHEVLAVCAPALHPGPDPQ
CCHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHH
AIASCPLIEVIGVTDTWHHFFALHGLAPPSSGPALKVDQSLIALELATRGMGVALIARVF
HHHCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCEEECHHHHHHHHHHCCCHHHHHHHHH
AQRYLESGALVEATDLALPAREGHYIVQPEARNHFRPEVAALKDWLLQEAAQTGC
HHHHHHCCCEEEEHHCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYQELPPLPWLRAFDASARLGNFTLAAQELGLTPSAVSYQVRGLEATLGHKLFVRKQKSL
CCCCCCCCCHHHHCCCCCCCCCEEEEHHHHCCCCCHHEEEEECHHHHHCCHHHHHHHHHH
FLTRLGQAYLPVVSKAFGDLEATTSNLFGKSAAQEITLRCLSSLNMLWLAPRLSRFQAQH
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCC
PDCRLRVLSALWGETPEALQIDIDIRYGNGSWSDGEVTRLMHHEVLAVCAPALHPGPDPQ
CCHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHH
AIASCPLIEVIGVTDTWHHFFALHGLAPPSSGPALKVDQSLIALELATRGMGVALIARVF
HHHCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCEEECHHHHHHHHHHCCCHHHHHHHHH
AQRYLESGALVEATDLALPAREGHYIVQPEARNHFRPEVAALKDWLLQEAAQTGC
HHHHHHCCCEEEEHHCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]