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The map label for this gene is pfkA [H]

Identifier: 94985737

GI number: 94985737

Start: 1741762

End: 1742772

Strand: Reverse

Name: pfkA [H]

Synonym: Dgeo_1637

Alternate gene names: 94985737

Gene position: 1742772-1741762 (Counterclockwise)

Preceding gene: 94985738

Following gene: 94985728

Centisome position: 70.64

GC content: 67.06

Gene sequence:

>1011_bases
GTGACTGACATTCCCCACCTGTCCACCCATGCCAACCCCGCCGGAATCAAGCGCCTGGCCGTCCTGACAAGTGGTGGCGA
CGCGCCGGGGATGAACGCCGCCATTCGCGCCGTCGTCCGCACTGCCACCTATCAGGGCCTGGAGGTGGTGGGTGTGCGCC
GGGGCTTTTCCGGCCTGCACCGCGGTGACCTGCGCCTGATCGGGCCAAGAGATGTGGCGAACACCATCCAGCGCGGCGGC
ACCATCCTGCTCACCGCCCGCTCGCACACCTGGCGCACACCCGAGGGCCGCGCTCGCGGCGCGCAGCATCTGCGCGACTG
GGGCGTGGACGGCCTGATCGTGATCGGCGGTGACGGCAGCTTTCACGGCGCGCACCTCCTCCAGCAGGAACACGGCATCC
CGGTGATCGGAGTTCCGGGCACCATCGACAATGACCTCTACGGCACTGACCACACCATCGGGTACTTCACGGCGGTTGAG
ACAGCCCTGGACGCCGTGGACAAACTGCGCGACACCGGCGCCAGCCACGAGCGCATCTTTGTGATCGAGGTGATGGGCCG
CCATGCCGGACACATCGCGTTGGAGGTTGCGGTCGCGGGCGGCGCCGAGGAAGTCTTTATCCCCGAAGACCCCAAGCCGG
TGGACGGCATCGTGCAGATCGTGCAGGACAGTGTGGCAAAGGGCAAGGCCAGTTCGATCATCATCGTCGCCGAGGGGTAC
CCGGGTGGGGCGCTGGGCGTCGCGCAGGCCATCGAGGTGGGCACCGGGCTGGAAACGCGGGTCAGCATCCTGGGCCACAT
CCAACGTGGCGGCTCACCGGTGAGCAGTGACCGCGTCCTGGCAAGCCGTCTGGGAGAAGCCGCCGTCTACGCTCTGATGG
ATGGATACAGCGATGTGATGGTGGGCCGCCGCAACGGCGACATCAGCCATACGCCGCTTCCCGAAACCTGGGAGAAGCGC
AAGGACGTGAGCCGCGACCTCTACCGCTGCGCCAAGACGCTGAGTGTCTGA

Upstream 100 bases:

>100_bases
TGCTCATGGCACAGGGTTTGCGTAAGAATGACGCTTATGCGCTGGCCCTTCAGGTCACGCAGGACGCCTGAACTTCTTGC
CCTCCTTCCGAGGATTTCCT

Downstream 100 bases:

>100_bases
AGTATCTGAAGGAGGCAAAAAGCGGGCCGCCCCGTTCGCTGTGGGGCGGCCCGCGTCTTCTGCTGGTCAGGATTTGGGAG
GCTTCACGGTCAGGGTGGTG

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MTDIPHLSTHANPAGIKRLAVLTSGGDAPGMNAAIRAVVRTATYQGLEVVGVRRGFSGLHRGDLRLIGPRDVANTIQRGG
TILLTARSHTWRTPEGRARGAQHLRDWGVDGLIVIGGDGSFHGAHLLQQEHGIPVIGVPGTIDNDLYGTDHTIGYFTAVE
TALDAVDKLRDTGASHERIFVIEVMGRHAGHIALEVAVAGGAEEVFIPEDPKPVDGIVQIVQDSVAKGKASSIIIVAEGY
PGGALGVAQAIEVGTGLETRVSILGHIQRGGSPVSSDRVLASRLGEAAVYALMDGYSDVMVGRRNGDISHTPLPETWEKR
KDVSRDLYRCAKTLSV

Sequences:

>Translated_336_residues
MTDIPHLSTHANPAGIKRLAVLTSGGDAPGMNAAIRAVVRTATYQGLEVVGVRRGFSGLHRGDLRLIGPRDVANTIQRGG
TILLTARSHTWRTPEGRARGAQHLRDWGVDGLIVIGGDGSFHGAHLLQQEHGIPVIGVPGTIDNDLYGTDHTIGYFTAVE
TALDAVDKLRDTGASHERIFVIEVMGRHAGHIALEVAVAGGAEEVFIPEDPKPVDGIVQIVQDSVAKGKASSIIIVAEGY
PGGALGVAQAIEVGTGLETRVSILGHIQRGGSPVSSDRVLASRLGEAAVYALMDGYSDVMVGRRNGDISHTPLPETWEKR
KDVSRDLYRCAKTLSV
>Mature_335_residues
TDIPHLSTHANPAGIKRLAVLTSGGDAPGMNAAIRAVVRTATYQGLEVVGVRRGFSGLHRGDLRLIGPRDVANTIQRGGT
ILLTARSHTWRTPEGRARGAQHLRDWGVDGLIVIGGDGSFHGAHLLQQEHGIPVIGVPGTIDNDLYGTDHTIGYFTAVET
ALDAVDKLRDTGASHERIFVIEVMGRHAGHIALEVAVAGGAEEVFIPEDPKPVDGIVQIVQDSVAKGKASSIIIVAEGYP
GGALGVAQAIEVGTGLETRVSILGHIQRGGSPVSSDRVLASRLGEAAVYALMDGYSDVMVGRRNGDISHTPLPETWEKRK
DVSRDLYRCAKTLSV

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI11321601, Length=355, Percent_Identity=39.7183098591549, Blast_Score=218, Evalue=8e-57,
Organism=Homo sapiens, GI48762920, Length=348, Percent_Identity=38.5057471264368, Blast_Score=211, Evalue=6e-55,
Organism=Homo sapiens, GI266453768, Length=347, Percent_Identity=38.0403458213257, Blast_Score=209, Evalue=3e-54,
Organism=Homo sapiens, GI266453748, Length=347, Percent_Identity=38.0403458213257, Blast_Score=209, Evalue=3e-54,
Organism=Homo sapiens, GI4505749, Length=347, Percent_Identity=38.0403458213257, Blast_Score=209, Evalue=3e-54,
Organism=Homo sapiens, GI266453619, Length=347, Percent_Identity=38.0403458213257, Blast_Score=209, Evalue=4e-54,
Organism=Escherichia coli, GI1790350, Length=320, Percent_Identity=47.5, Blast_Score=275, Evalue=3e-75,
Organism=Caenorhabditis elegans, GI25147584, Length=311, Percent_Identity=39.5498392282958, Blast_Score=206, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17558788, Length=311, Percent_Identity=38.2636655948553, Blast_Score=189, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI72003601, Length=289, Percent_Identity=30.1038062283737, Blast_Score=134, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6321679, Length=344, Percent_Identity=39.8255813953488, Blast_Score=221, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6323861, Length=346, Percent_Identity=37.8612716763006, Blast_Score=206, Evalue=5e-54,
Organism=Drosophila melanogaster, GI28573326, Length=347, Percent_Identity=37.7521613832853, Blast_Score=219, Evalue=3e-57,
Organism=Drosophila melanogaster, GI17647809, Length=364, Percent_Identity=37.3626373626374, Blast_Score=218, Evalue=3e-57,
Organism=Drosophila melanogaster, GI24652337, Length=348, Percent_Identity=38.2183908045977, Blast_Score=216, Evalue=2e-56,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 35548; Mature: 35417

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDIPHLSTHANPAGIKRLAVLTSGGDAPGMNAAIRAVVRTATYQGLEVVGVRRGFSGLH
CCCCCCCCCCCCHHHHEEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEEHHCCCCCC
RGDLRLIGPRDVANTIQRGGTILLTARSHTWRTPEGRARGAQHLRDWGVDGLIVIGGDGS
CCCEEEECCHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCC
FHGAHLLQQEHGIPVIGVPGTIDNDLYGTDHTIGYFTAVETALDAVDKLRDTGASHERIF
CCHHHHHHHHCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEE
VIEVMGRHAGHIALEVAVAGGAEEVFIPEDPKPVDGIVQIVQDSVAKGKASSIIIVAEGY
EEEEECCCCCEEEEEEEEECCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCC
PGGALGVAQAIEVGTGLETRVSILGHIQRGGSPVSSDRVLASRLGEAAVYALMDGYSDVM
CCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHE
VGRRNGDISHTPLPETWEKRKDVSRDLYRCAKTLSV
EECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TDIPHLSTHANPAGIKRLAVLTSGGDAPGMNAAIRAVVRTATYQGLEVVGVRRGFSGLH
CCCCCCCCCCCHHHHEEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEEHHCCCCCC
RGDLRLIGPRDVANTIQRGGTILLTARSHTWRTPEGRARGAQHLRDWGVDGLIVIGGDGS
CCCEEEECCHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCC
FHGAHLLQQEHGIPVIGVPGTIDNDLYGTDHTIGYFTAVETALDAVDKLRDTGASHERIF
CCHHHHHHHHCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEE
VIEVMGRHAGHIALEVAVAGGAEEVFIPEDPKPVDGIVQIVQDSVAKGKASSIIIVAEGY
EEEEECCCCCEEEEEEEEECCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCC
PGGALGVAQAIEVGTGLETRVSILGHIQRGGSPVSSDRVLASRLGEAAVYALMDGYSDVM
CCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHE
VGRRNGDISHTPLPETWEKRKDVSRDLYRCAKTLSV
EECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]