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The map label for this gene is purU [H]

Identifier: 94985588

GI number: 94985588

Start: 1572380

End: 1573270

Strand: Reverse

Name: purU [H]

Synonym: Dgeo_1488

Alternate gene names: 94985588

Gene position: 1573270-1572380 (Counterclockwise)

Preceding gene: 94985590

Following gene: 94985579

Centisome position: 63.77

GC content: 64.2

Gene sequence:

>891_bases
ATGACGGCCCCCTCCTCTTCCTCCAGGCTTGATCCCCTCAACACCGCCGTCCTCACCATCACCTGCCCGGACCGGGGCGG
CATCGTGGCGGCGGTGTCGCAGTTTCTTTTTAGCCACGGCGCGAACATCCTCCACTCCGACCAGCACTCCACTGACCCCG
CAGGCGGCACCTTTTTCATGCGGATGGAGTTTCACCTCGATGGCCTCGATCTGGCGCGCGAGCCGTTCGAGCGGGCCTTT
GCGCAGGTCATCGCCGCCCCCTTTGGCATGGACTGGCGCCTGAGCTACACGGCTCAGCCCAAGCGCATGGCGATTTTGGT
GAGCCGCTACGACCACTGCTTTTTGGATCTGCTGTGGCGCAGGCGCCGGGGCGAACTGAATGTGGAAATTCCCCTCGTGA
TCAGTAACCACCCGGACCTCGCCCGAGACGCCGACATGTTCGGCATTCCCTTTCACGTGGTCCCCGTAACGCGGGAGAAC
AAGGCAGAGGCCGAAGCCGAGCAGGTGCGGTTGCTGCAGGAAGCCGGAGCCGACTTCGCCGTTCTCGCGCGCTACATGCA
GATTCTCAGCGGTGACTTCCTGCGCGAGTTTGGGCGTCCGGTCATCAACATCCACCACTCGTTCCTGCCGGCCTTTGTGG
GAGCCAACCCCTACCGCGCCGCCTTTCAGCGCGGCGTAAAGCTCATTGGCGCGACCAGCCACTACGTGACGGAAGAACTC
GACGCCGGGCCGATCATCGCCCAGGACGTGATTCCCGTGACCCACCGTGAGACTCCCGACACCCTGATGCGCCTGGGCCG
CGACGTGGAACGCCAGGTGCTCGCTCGCGCCGTCAAGGCCCACGTGGAAGACCGGGTGCTGGTGCACGGCAACAAGACGG
TGGTGTTTTAG

Upstream 100 bases:

>100_bases
CGTGCCGGGCTGGGTCGTAGCTCAGGGGGGAAGCCGCCTCGCGATTCGTCTGCACGCGCCCGAGCCTAGCACCCCGGAGC
AGCGGCTATCCTGCGCCCAC

Downstream 100 bases:

>100_bases
AGCGTGGTGTTTGAGAAAGGGTGAGGGATGGAGTGCCCCCCTTAATTCAGCTCCCGCGCTTCTCCCGGCATGAAGCCCAG
GAGCGTTTCCAGGCGGCCCA

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MTAPSSSSRLDPLNTAVLTITCPDRGGIVAAVSQFLFSHGANILHSDQHSTDPAGGTFFMRMEFHLDGLDLAREPFERAF
AQVIAAPFGMDWRLSYTAQPKRMAILVSRYDHCFLDLLWRRRRGELNVEIPLVISNHPDLARDADMFGIPFHVVPVTREN
KAEAEAEQVRLLQEAGADFAVLARYMQILSGDFLREFGRPVINIHHSFLPAFVGANPYRAAFQRGVKLIGATSHYVTEEL
DAGPIIAQDVIPVTHRETPDTLMRLGRDVERQVLARAVKAHVEDRVLVHGNKTVVF

Sequences:

>Translated_296_residues
MTAPSSSSRLDPLNTAVLTITCPDRGGIVAAVSQFLFSHGANILHSDQHSTDPAGGTFFMRMEFHLDGLDLAREPFERAF
AQVIAAPFGMDWRLSYTAQPKRMAILVSRYDHCFLDLLWRRRRGELNVEIPLVISNHPDLARDADMFGIPFHVVPVTREN
KAEAEAEQVRLLQEAGADFAVLARYMQILSGDFLREFGRPVINIHHSFLPAFVGANPYRAAFQRGVKLIGATSHYVTEEL
DAGPIIAQDVIPVTHRETPDTLMRLGRDVERQVLARAVKAHVEDRVLVHGNKTVVF
>Mature_295_residues
TAPSSSSRLDPLNTAVLTITCPDRGGIVAAVSQFLFSHGANILHSDQHSTDPAGGTFFMRMEFHLDGLDLAREPFERAFA
QVIAAPFGMDWRLSYTAQPKRMAILVSRYDHCFLDLLWRRRRGELNVEIPLVISNHPDLARDADMFGIPFHVVPVTRENK
AEAEAEQVRLLQEAGADFAVLARYMQILSGDFLREFGRPVINIHHSFLPAFVGANPYRAAFQRGVKLIGATSHYVTEELD
AGPIIAQDVIPVTHRETPDTLMRLGRDVERQVLARAVKAHVEDRVLVHGNKTVVF

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=206, Percent_Identity=30.5825242718447, Blast_Score=101, Evalue=9e-22,
Organism=Homo sapiens, GI209869995, Length=206, Percent_Identity=30.5825242718447, Blast_Score=101, Evalue=9e-22,
Organism=Homo sapiens, GI209869993, Length=206, Percent_Identity=30.5825242718447, Blast_Score=101, Evalue=9e-22,
Organism=Escherichia coli, GI1787483, Length=290, Percent_Identity=40.6896551724138, Blast_Score=207, Evalue=5e-55,
Organism=Escherichia coli, GI1788846, Length=145, Percent_Identity=29.6551724137931, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17567511, Length=192, Percent_Identity=34.8958333333333, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24582400, Length=195, Percent_Identity=31.2820512820513, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 33073; Mature: 32942

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPSSSSRLDPLNTAVLTITCPDRGGIVAAVSQFLFSHGANILHSDQHSTDPAGGTFFM
CCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEE
RMEFHLDGLDLAREPFERAFAQVIAAPFGMDWRLSYTAQPKRMAILVSRYDHCFLDLLWR
EEEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEHHHHHHHHHHHHHHHH
RRRGELNVEIPLVISNHPDLARDADMFGIPFHVVPVTRENKAEAEAEQVRLLQEAGADFA
HHCCCEEEEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCHH
VLARYMQILSGDFLREFGRPVINIHHSFLPAFVGANPYRAAFQRGVKLIGATSHYVTEEL
HHHHHHHHHCCHHHHHHCCCEEEHHHHHHHHHHCCCHHHHHHHCCCEEEECCHHHHHHHC
DAGPIIAQDVIPVTHRETPDTLMRLGRDVERQVLARAVKAHVEDRVLVHGNKTVVF
CCCCEEHHCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCEEEEC
>Mature Secondary Structure 
TAPSSSSRLDPLNTAVLTITCPDRGGIVAAVSQFLFSHGANILHSDQHSTDPAGGTFFM
CCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEE
RMEFHLDGLDLAREPFERAFAQVIAAPFGMDWRLSYTAQPKRMAILVSRYDHCFLDLLWR
EEEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEHHHHHHHHHHHHHHHH
RRRGELNVEIPLVISNHPDLARDADMFGIPFHVVPVTRENKAEAEAEQVRLLQEAGADFA
HHCCCEEEEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCHH
VLARYMQILSGDFLREFGRPVINIHHSFLPAFVGANPYRAAFQRGVKLIGATSHYVTEEL
HHHHHHHHHCCHHHHHHCCCEEEHHHHHHHHHHCCCHHHHHHHCCCEEEECCHHHHHHHC
DAGPIIAQDVIPVTHRETPDTLMRLGRDVERQVLARAVKAHVEDRVLVHGNKTVVF
CCCCEEHHCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]