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The map label for this gene is fusA [H]

Identifier: 94985553

GI number: 94985553

Start: 1540618

End: 1542651

Strand: Reverse

Name: fusA [H]

Synonym: Dgeo_1453

Alternate gene names: 94985553

Gene position: 1542651-1540618 (Counterclockwise)

Preceding gene: 94985559

Following gene: 94985552

Centisome position: 62.53

GC content: 67.65

Gene sequence:

>2034_bases
ATGCCGTCTGAGTCCGTTGTCCCCGAAAACACTCCCGTTCGGGTTGTGAGTCTTGCCGGCCACAGCGGCGCGGGAAAAAC
CACCCTCGCGGAGGCCCTGCTCGTCGCCAGCGGGGCCTTGCTCCGGGCGGGCCGGGTCACCGAAGGCACCACCCAGAGTG
ATCACACCGACGCCGAGAAACAGCACGGGTTTTCCATCACGACCGGGGTGCTGCGGCTGAGTCAGGGTGGTACGGACATC
ACGCTGCTCGACACGCCGGGCTACGCCGACTTCGTGCGGGAGATTCGCGGCGGCATTCGCGCGGCGGACTCCGTGCTGAT
GGTGGTCAGCGCCGTGAGCGGCGTGGAGGTCGGCACCGAGCGGGTCTGGGCCACCGCTGACCGCTTCGAAATGCCGCGCC
TCGTGGTGATCTCCAAGATGGACCGCGAACGCGCCAACTTCCACGCGGTCCTGGCCGATCTCCGTGCCAGCTTGCGGGGT
CCGGTGGCAGCGGCGTACCTGCCGGTGGGCGAGGGGCCAGCATTCCGGGGCGTGGTGGATGTGCTGACAGCAGCTCCCGA
CGAGCTGCCCGGCGAGCTGCGCCCTGCGCTCAACGAGGCCCGCGAGGCGCTGGTGGACGCCATTGTCGAAACCGACGATG
ACCTCATGAACCGGTACCTGGAAGGAGAGGCGATCGGCACGGAGGAGTTGCGTGCGGCGTTGTTGCGCGCTGTTCACGCA
GGGAGGCTCTACCCGGTCGTTCCGGTGAGCGCTGAGACCGGCGTGGGCGTGCCCGAGCTGCTCAACCTGATGGTGGTGGG
GCTGCGGAGTGCCTCCGAACGTGGCGTGCTGACCGGGGTGGACGGGCAGACGCGCGAGCCCACACCGAACGCGCCTGCCA
GTGCCCGCGTGTGGCGCGTCAGCGTGGACCCCTTTGTGGGCAAGCTGGCCTATATCCGTGTTTGGAGCGGCACCATCCGC
CCCGGCGACACGCTGCGCAACACGACGCGCGGCGTGGACGTGAAGCCCGCCCACCTGTATGTGGTGAGCGGCAAGGACCT
CACCGAGGTGTCCGAACTGCGCGCCGGGATGATCGGCGTGCTGACCAAAATCCCCGAGCTGCACACTGGCGACACCCTGG
CCGACCCCGCTCACCCCATTGAGTACGACCCCCTGGTGCTGCCCGACCCGGCTCATACCGTGGCCCTCTTTCCCAAGACC
CGCCAGGACGAGGACCGCCTCAGCAGCGCCATCGGCCGTCTTCTCGACGAGGATCCCACCCTGCGCTTTGCGCGCGAGGG
GCAGACCGGCGAGCTGCTCCTCTCCGGGATGGGTGACATGCACACGACCATCGCGGTGGAGAAGCTCGCCGCACTCGGCG
TGAATGTGGACACGGGTGTTCCGCAGATTCCCTACCGCGAAACCATCCGCGCTGTCTCGCAGGCCCAGGGCAAGCACAAG
AAGCAGTCGGGTGGACACGGCCAGTACGGCGACTGCCATCTGCGTGTGGAGCCGCGTGAGGGCTTCAGCTTCTGCTCGGA
GGTGGTGGGCGGCGCCATTCCCGGCAAGTACCTCCCCAGCATCGAGAAGGGTGTGCAAGACGCCATGCAGAAGGGGCCAC
TGGCGGGTTACCCGATGCAGGACGTTCACGTAGCCGTCACCCACGGGTCCTACCACGACGTGGACTCCAGCGACCTCGCC
TTCCGCACCGCTGGGGCGCTGGCCTTCCGCAACGCGGTGGAGGGGGCCAAACCGGTCTTGCTTGAGCCGGTGATGCTTCT
CAAGGTGCGTGCCCCCGCTCAGTTCACTGGCGACCTGATCGGTGACCTTCAGACCCGCCGTGCCCGCGTGCAGGGGATGG
ACCCCGCAGGGACCGTGATCACCATCACTGCCGTCGTCCCGCAGTCGGAGTTGCAGACCTACAGCGCCGACCTGCGCTCC
ATGACGGGTGACCGGGGTGCCTTCAGCGTGAAGCCCGCTGGCTACCAGGACCTTCCCGAACACCTGGCCAAGAAGGTGAT
CGAGGCGCGCAAGGCGGCGAGCCTTTCATCGTAA

Upstream 100 bases:

>100_bases
ATGCTCCTCATACCTCCAACTGGTTCACTCGTCTGGTGGCCGTTCGTCTGCCCTCCTCTTTTCTGGCTGCACTGCTCTGG
AAAGGGTCCGGTGATGTGGA

Downstream 100 bases:

>100_bases
GGGATCTGTGAGCTGTCGACTCGGAGAATGAGACCAGCAGGTGACGCCCAGTGCCTCACGGTGCAGAGCACCTCACACAG
AGACTCCCGTACACCGGGAG

Product: elongation factor G

Products: GDP; phosphate

Alternate protein names: EF-G [H]

Number of amino acids: Translated: 677; Mature: 676

Protein sequence:

>677_residues
MPSESVVPENTPVRVVSLAGHSGAGKTTLAEALLVASGALLRAGRVTEGTTQSDHTDAEKQHGFSITTGVLRLSQGGTDI
TLLDTPGYADFVREIRGGIRAADSVLMVVSAVSGVEVGTERVWATADRFEMPRLVVISKMDRERANFHAVLADLRASLRG
PVAAAYLPVGEGPAFRGVVDVLTAAPDELPGELRPALNEAREALVDAIVETDDDLMNRYLEGEAIGTEELRAALLRAVHA
GRLYPVVPVSAETGVGVPELLNLMVVGLRSASERGVLTGVDGQTREPTPNAPASARVWRVSVDPFVGKLAYIRVWSGTIR
PGDTLRNTTRGVDVKPAHLYVVSGKDLTEVSELRAGMIGVLTKIPELHTGDTLADPAHPIEYDPLVLPDPAHTVALFPKT
RQDEDRLSSAIGRLLDEDPTLRFAREGQTGELLLSGMGDMHTTIAVEKLAALGVNVDTGVPQIPYRETIRAVSQAQGKHK
KQSGGHGQYGDCHLRVEPREGFSFCSEVVGGAIPGKYLPSIEKGVQDAMQKGPLAGYPMQDVHVAVTHGSYHDVDSSDLA
FRTAGALAFRNAVEGAKPVLLEPVMLLKVRAPAQFTGDLIGDLQTRRARVQGMDPAGTVITITAVVPQSELQTYSADLRS
MTGDRGAFSVKPAGYQDLPEHLAKKVIEARKAASLSS

Sequences:

>Translated_677_residues
MPSESVVPENTPVRVVSLAGHSGAGKTTLAEALLVASGALLRAGRVTEGTTQSDHTDAEKQHGFSITTGVLRLSQGGTDI
TLLDTPGYADFVREIRGGIRAADSVLMVVSAVSGVEVGTERVWATADRFEMPRLVVISKMDRERANFHAVLADLRASLRG
PVAAAYLPVGEGPAFRGVVDVLTAAPDELPGELRPALNEAREALVDAIVETDDDLMNRYLEGEAIGTEELRAALLRAVHA
GRLYPVVPVSAETGVGVPELLNLMVVGLRSASERGVLTGVDGQTREPTPNAPASARVWRVSVDPFVGKLAYIRVWSGTIR
PGDTLRNTTRGVDVKPAHLYVVSGKDLTEVSELRAGMIGVLTKIPELHTGDTLADPAHPIEYDPLVLPDPAHTVALFPKT
RQDEDRLSSAIGRLLDEDPTLRFAREGQTGELLLSGMGDMHTTIAVEKLAALGVNVDTGVPQIPYRETIRAVSQAQGKHK
KQSGGHGQYGDCHLRVEPREGFSFCSEVVGGAIPGKYLPSIEKGVQDAMQKGPLAGYPMQDVHVAVTHGSYHDVDSSDLA
FRTAGALAFRNAVEGAKPVLLEPVMLLKVRAPAQFTGDLIGDLQTRRARVQGMDPAGTVITITAVVPQSELQTYSADLRS
MTGDRGAFSVKPAGYQDLPEHLAKKVIEARKAASLSS
>Mature_676_residues
PSESVVPENTPVRVVSLAGHSGAGKTTLAEALLVASGALLRAGRVTEGTTQSDHTDAEKQHGFSITTGVLRLSQGGTDIT
LLDTPGYADFVREIRGGIRAADSVLMVVSAVSGVEVGTERVWATADRFEMPRLVVISKMDRERANFHAVLADLRASLRGP
VAAAYLPVGEGPAFRGVVDVLTAAPDELPGELRPALNEAREALVDAIVETDDDLMNRYLEGEAIGTEELRAALLRAVHAG
RLYPVVPVSAETGVGVPELLNLMVVGLRSASERGVLTGVDGQTREPTPNAPASARVWRVSVDPFVGKLAYIRVWSGTIRP
GDTLRNTTRGVDVKPAHLYVVSGKDLTEVSELRAGMIGVLTKIPELHTGDTLADPAHPIEYDPLVLPDPAHTVALFPKTR
QDEDRLSSAIGRLLDEDPTLRFAREGQTGELLLSGMGDMHTTIAVEKLAALGVNVDTGVPQIPYRETIRAVSQAQGKHKK
QSGGHGQYGDCHLRVEPREGFSFCSEVVGGAIPGKYLPSIEKGVQDAMQKGPLAGYPMQDVHVAVTHGSYHDVDSSDLAF
RTAGALAFRNAVEGAKPVLLEPVMLLKVRAPAQFTGDLIGDLQTRRARVQGMDPAGTVITITAVVPQSELQTYSADLRSM
TGDRGAFSVKPAGYQDLPEHLAKKVIEARKAASLSS

Specific function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=705, Percent_Identity=30.6382978723404, Blast_Score=328, Evalue=1e-89,
Organism=Homo sapiens, GI19923640, Length=710, Percent_Identity=25.6338028169014, Blast_Score=217, Evalue=2e-56,
Organism=Homo sapiens, GI25306287, Length=685, Percent_Identity=25.6934306569343, Blast_Score=179, Evalue=1e-44,
Organism=Homo sapiens, GI25306283, Length=394, Percent_Identity=28.4263959390863, Blast_Score=143, Evalue=5e-34,
Organism=Escherichia coli, GI1789738, Length=698, Percent_Identity=34.6704871060172, Blast_Score=444, Evalue=1e-125,
Organism=Escherichia coli, GI1790835, Length=376, Percent_Identity=27.3936170212766, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI48994988, Length=130, Percent_Identity=33.8461538461538, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17533571, Length=692, Percent_Identity=28.757225433526, Blast_Score=296, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI17556745, Length=716, Percent_Identity=22.9050279329609, Blast_Score=188, Evalue=7e-48,
Organism=Saccharomyces cerevisiae, GI6323098, Length=688, Percent_Identity=30.8139534883721, Blast_Score=323, Evalue=5e-89,
Organism=Saccharomyces cerevisiae, GI6322359, Length=772, Percent_Identity=25.259067357513, Blast_Score=214, Evalue=4e-56,
Organism=Drosophila melanogaster, GI24582462, Length=714, Percent_Identity=28.8515406162465, Blast_Score=313, Evalue=2e-85,
Organism=Drosophila melanogaster, GI221458488, Length=721, Percent_Identity=25.7975034674064, Blast_Score=213, Evalue=3e-55,
Organism=Drosophila melanogaster, GI78706572, Length=148, Percent_Identity=31.0810810810811, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR004540
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 72090; Mature: 71959

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSESVVPENTPVRVVSLAGHSGAGKTTLAEALLVASGALLRAGRVTEGTTQSDHTDAEK
CCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCHHH
QHGFSITTGVLRLSQGGTDITLLDTPGYADFVREIRGGIRAADSVLMVVSAVSGVEVGTE
HCCCEEEEHHEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
RVWATADRFEMPRLVVISKMDRERANFHAVLADLRASLRGPVAAAYLPVGEGPAFRGVVD
HHEECHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHH
VLTAAPDELPGELRPALNEAREALVDAIVETDDDLMNRYLEGEAIGTEELRAALLRAVHA
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHC
GRLYPVVPVSAETGVGVPELLNLMVVGLRSASERGVLTGVDGQTREPTPNAPASARVWRV
CCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCCCCCEEEEE
SVDPFVGKLAYIRVWSGTIRPGDTLRNTTRGVDVKPAHLYVVSGKDLTEVSELRAGMIGV
ECCHHHCCEEEEEEECCCCCCCHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHH
LTKIPELHTGDTLADPAHPIEYDPLVLPDPAHTVALFPKTRQDEDRLSSAIGRLLDEDPT
HHHCCCCCCCCCCCCCCCCCCCCCEECCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCC
LRFAREGQTGELLLSGMGDMHTTIAVEKLAALGVNVDTGVPQIPYRETIRAVSQAQGKHK
EEEECCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCHH
KQSGGHGQYGDCHLRVEPREGFSFCSEVVGGAIPGKYLPSIEKGVQDAMQKGPLAGYPMQ
HCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
DVHVAVTHGSYHDVDSSDLAFRTAGALAFRNAVEGAKPVLLEPVMLLKVRAPAQFTGDLI
CEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHCCEEEEEECCCCHHHHHHH
GDLQTRRARVQGMDPAGTVITITAVVPQSELQTYSADLRSMTGDRGAFSVKPAGYQDLPE
HHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHH
HLAKKVIEARKAASLSS
HHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PSESVVPENTPVRVVSLAGHSGAGKTTLAEALLVASGALLRAGRVTEGTTQSDHTDAEK
CCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCHHH
QHGFSITTGVLRLSQGGTDITLLDTPGYADFVREIRGGIRAADSVLMVVSAVSGVEVGTE
HCCCEEEEHHEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
RVWATADRFEMPRLVVISKMDRERANFHAVLADLRASLRGPVAAAYLPVGEGPAFRGVVD
HHEECHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHH
VLTAAPDELPGELRPALNEAREALVDAIVETDDDLMNRYLEGEAIGTEELRAALLRAVHA
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHC
GRLYPVVPVSAETGVGVPELLNLMVVGLRSASERGVLTGVDGQTREPTPNAPASARVWRV
CCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCCCCCEEEEE
SVDPFVGKLAYIRVWSGTIRPGDTLRNTTRGVDVKPAHLYVVSGKDLTEVSELRAGMIGV
ECCHHHCCEEEEEEECCCCCCCHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHH
LTKIPELHTGDTLADPAHPIEYDPLVLPDPAHTVALFPKTRQDEDRLSSAIGRLLDEDPT
HHHCCCCCCCCCCCCCCCCCCCCCEECCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCC
LRFAREGQTGELLLSGMGDMHTTIAVEKLAALGVNVDTGVPQIPYRETIRAVSQAQGKHK
EEEECCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCHH
KQSGGHGQYGDCHLRVEPREGFSFCSEVVGGAIPGKYLPSIEKGVQDAMQKGPLAGYPMQ
HCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
DVHVAVTHGSYHDVDSSDLAFRTAGALAFRNAVEGAKPVLLEPVMLLKVRAPAQFTGDLI
CEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHCCEEEEEECCCCHHHHHHH
GDLQTRRARVQGMDPAGTVITITAVVPQSELQTYSADLRSMTGDRGAFSVKPAGYQDLPE
HHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHH
HLAKKVIEARKAASLSS
HHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA