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The map label for this gene is yagI [H]

Identifier: 94972244

GI number: 94972244

Start: 248019

End: 248780

Strand: Direct

Name: yagI [H]

Synonym: Dgeo_2778

Alternate gene names: 94972244

Gene position: 248019-248780 (Clockwise)

Preceding gene: 94972251

Following gene: 94972233

Centisome position: 43.2

GC content: 67.32

Gene sequence:

>762_bases
ATGCTGGGAACCCTAGAAAAAGCCGGGCAGGTGCTGGACCTGTATTCGGATGAGCGGCCGGAGTGGGGAGCGACCGGGGT
GGCCGCGGCGCTGGGGATTCCCAAGGCCAGCGCGCACCACTTGCTCGCCTCGCTGGCCGAGATCGGCCTCCTGCACCGCA
CCGCCAATCGGCAATACCGGCTAGGCTTCAAGGCCCTCGTTCTCAGTCGCATCTTGCTGCGGGCGACCCCGTGGCGCGAG
GCGGCCGAGCAGGAGATGCGAACCCTGACGCAGGAGTTCGGCGAGTCGGTGCAACTGGGCTCGCTGGACGGCAACCGCCT
GGTGTGCGTGGCCCGGCTGGGCGGGACGCTGCCGGATAGCGTGCATATCGCGGATGTCGGGACGAGCATCCCGCCCCATT
GCAGCTCGAACGGCAAGGTGCTGCTGGCCTTCAGCCCCAGCGAGCGCGCGCTGCCGGTGCTGCAACACCAGGGCCTGACG
CGCTTCACCGAGAACACTATCACCACCCCCGACGAGCTGGCCTCCGAACTGGCGCGCGTTCGCGAGCTGGGCTTCGCTTA
CGACGTAGGCGAGTATCGCGACAACTTCTGTTCTGTCGGCGCCCCGATCCGCAATCACCTTGGCGAGGTGGTGGCGTCCT
TGAGCATCGCCACGCCCGCGCGCCGCTTCTACGAGCGGAAGGCAGCCTATCGCACGGCGGTTATCCGCGTCACTGAGCGC
ATCTCCATGCGGATCGGCTACAACCCAACGCCGCTCTGGTAG

Upstream 100 bases:

>100_bases
GTTATGTACCCCCCACTTTGAGGCAGGGCCGCCCCCCTGACAATCCTCTTGTTCCATATACTGAAACCCTGAACCCTACC
AAGGGAGCAGGAGGTCCGCC

Downstream 100 bases:

>100_bases
AGTTGTGGGCGAGCGGTATATCTCCGCCCTCCCCAGCAAATCTACTGCCCGCTTCGTGAATTGCACAGTACGCCGTTTAA
CAGGACTTTGTCATTCTCCT

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MLGTLEKAGQVLDLYSDERPEWGATGVAAALGIPKASAHHLLASLAEIGLLHRTANRQYRLGFKALVLSRILLRATPWRE
AAEQEMRTLTQEFGESVQLGSLDGNRLVCVARLGGTLPDSVHIADVGTSIPPHCSSNGKVLLAFSPSERALPVLQHQGLT
RFTENTITTPDELASELARVRELGFAYDVGEYRDNFCSVGAPIRNHLGEVVASLSIATPARRFYERKAAYRTAVIRVTER
ISMRIGYNPTPLW

Sequences:

>Translated_253_residues
MLGTLEKAGQVLDLYSDERPEWGATGVAAALGIPKASAHHLLASLAEIGLLHRTANRQYRLGFKALVLSRILLRATPWRE
AAEQEMRTLTQEFGESVQLGSLDGNRLVCVARLGGTLPDSVHIADVGTSIPPHCSSNGKVLLAFSPSERALPVLQHQGLT
RFTENTITTPDELASELARVRELGFAYDVGEYRDNFCSVGAPIRNHLGEVVASLSIATPARRFYERKAAYRTAVIRVTER
ISMRIGYNPTPLW
>Mature_253_residues
MLGTLEKAGQVLDLYSDERPEWGATGVAAALGIPKASAHHLLASLAEIGLLHRTANRQYRLGFKALVLSRILLRATPWRE
AAEQEMRTLTQEFGESVQLGSLDGNRLVCVARLGGTLPDSVHIADVGTSIPPHCSSNGKVLLAFSPSERALPVLQHQGLT
RFTENTITTPDELASELARVRELGFAYDVGEYRDNFCSVGAPIRNHLGEVVASLSIATPARRFYERKAAYRTAVIRVTER
ISMRIGYNPTPLW

Specific function: Unknown

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1786468, Length=249, Percent_Identity=28.5140562248996, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI1788131, Length=237, Percent_Identity=26.5822784810127, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI87082359, Length=253, Percent_Identity=22.5296442687747, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1788581, Length=213, Percent_Identity=30.9859154929577, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786715, Length=168, Percent_Identity=30.3571428571429, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790752, Length=217, Percent_Identity=26.2672811059908, Blast_Score=64, Evalue=7e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 27730; Mature: 27730

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGTLEKAGQVLDLYSDERPEWGATGVAAALGIPKASAHHLLASLAEIGLLHRTANRQYR
CCCCHHHHCHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHH
LGFKALVLSRILLRATPWREAAEQEMRTLTQEFGESVQLGSLDGNRLVCVARLGGTLPDS
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCEEEEEEECCCCCCCC
VHIADVGTSIPPHCSSNGKVLLAFSPSERALPVLQHQGLTRFTENTITTPDELASELARV
EEEEECCCCCCCCCCCCCEEEEEECCCCCCCHHHHHCCCHHHHCCCCCCHHHHHHHHHHH
RELGFAYDVGEYRDNFCSVGAPIRNHLGEVVASLSIATPARRFYERKAAYRTAVIRVTER
HHHCCHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISMRIGYNPTPLW
HHEEECCCCCCCC
>Mature Secondary Structure
MLGTLEKAGQVLDLYSDERPEWGATGVAAALGIPKASAHHLLASLAEIGLLHRTANRQYR
CCCCHHHHCHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHH
LGFKALVLSRILLRATPWREAAEQEMRTLTQEFGESVQLGSLDGNRLVCVARLGGTLPDS
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCEEEEEEECCCCCCCC
VHIADVGTSIPPHCSSNGKVLLAFSPSERALPVLQHQGLTRFTENTITTPDELASELARV
EEEEECCCCCCCCCCCCCEEEEEECCCCCCCHHHHHCCCHHHHCCCCCCHHHHHHHHHHH
RELGFAYDVGEYRDNFCSVGAPIRNHLGEVVASLSIATPARRFYERKAAYRTAVIRVTER
HHHCCHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISMRIGYNPTPLW
HHEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]