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The map label for this gene is wcaI [H]

Identifier: 94314775

GI number: 94314775

Start: 50858

End: 52120

Strand: Reverse

Name: wcaI [H]

Synonym: Rmet_5856

Alternate gene names: 94314775

Gene position: 52120-50858 (Counterclockwise)

Preceding gene: 291481470

Following gene: 94314774

Centisome position: 2.02

GC content: 67.14

Gene sequence:

>1263_bases
ATGAAAATCCTGCTTTATTGCCTCAACTACGCGCCGGAACTGACTGGTATCGGCAAGTACACCGGAGAGCAGGCCGAATG
GCTTGCCGCGCGCGGGCACCAGGTGCGCGTGATCACGGCGCCGCCCTATTACCCTGCCTGGAAGATCGACCGGGCATACC
GGGGCCTGCGCTACCGGCGCGAGATGCGCCACGCCGTCGACGTCATGCGCGCGCCGCTGTGGGTGCCCGAGCGTCCGTCC
GGCGTGAAGCGACTGCTGCATCTCGCGTCGTTCGCCGTCACCAGTCTTGGATGGCTGGCACGCCAAGTGGCATGGCGCCC
CGACGTGGTGTTCACCGTCGAGCCGCCATTGTTCTGCAGCCCCGCGACGCTGTTGTTCGCCCGGCTCACCGGCGCGAAGG
CATGGCTGCATATCCAGGACTACGAGGTCGATGCCGCCTTCAAGCTGGGGCTGCTCAAGGGCGGCCTGCCGCAACGCATG
GCGAGTACGTTCGAGCGCTTCATCATGCGGCGCTATGACCATGTGTCATCGATCTCGCACACGATGGTGGCACTGGCACG
CCAGAAAGGCGTGGACGCCACGCGCGTGTCCATGCTGCCCAACTGGGTAGATCTGGCGGCACTGGCGCGCCGAGGCGACG
CGGATTTCCGCGCACGCCTTGGAATACCGGCGAACGCCGTCGTGGCGCTGTATTCCGGCACCATCGGCACCAAGCAGGGC
ATCGAGATCCTGGCCGACGCGGCGCGGCACCTGTGCTCGCGCGATGACCTTCATTTCATCATCTGCGGCGCGGGCGCCGG
AGTGCCGGCATTGCGCACGCAATGCGAAGGCCTGGCACGCGTGCATTTCCTCCCGCTGCAATCGGCCGAAGACTTCCCGG
CGCTGCTCGATTGCGCCGACATCCACCTGATGCCCCAGCGGGCCGACGCCGCCGACCTCGTGCTGCCGTCCAAGCTCACG
AACATGCTCGCCAGCGGCAAACCCGTGGTGGCCACTGCTGCACCGGACACCGAGCTGGGCAAGCTGGTCATTCAGTTCGG
CTCGCTGGTCGAACCCGGCAACAGCGTGGCAATGGCCGATGCCATCGCGGCGCTGGCCGACGATCCGCCGCGCCGTCAGG
CACTCGGGTCGGCCGGAAAGGCGTGGGCAGCCGCCCATCTCGACCGGGACGCCGTGCTGGCGGAATTCGAGCGAACGTTG
CTGCGATTGCGTGCCCCACGTGGCGTGGCCGATCAGGCCGCCGCCGACCTCGCCGGGAAATAA

Upstream 100 bases:

>100_bases
TCGCACGTGAATGAGCCGCCTTGTCGCACTGACCAACGGTGGATCCCGAATGGATACCTCCACCCTGACCAGCCCGGGGT
GGCAGTACTGAAGTAGACCG

Downstream 100 bases:

>100_bases
GGCCGGGCACACGTCATGTGACCGCTTTGTGGTCTGGCGTACGGTACGTACGCCAGCGCGGGGCCATGATGAAACCATCG
TCACCGGCCTGACCGGGAAC

Product: putative glycosyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MKILLYCLNYAPELTGIGKYTGEQAEWLAARGHQVRVITAPPYYPAWKIDRAYRGLRYRREMRHAVDVMRAPLWVPERPS
GVKRLLHLASFAVTSLGWLARQVAWRPDVVFTVEPPLFCSPATLLFARLTGAKAWLHIQDYEVDAAFKLGLLKGGLPQRM
ASTFERFIMRRYDHVSSISHTMVALARQKGVDATRVSMLPNWVDLAALARRGDADFRARLGIPANAVVALYSGTIGTKQG
IEILADAARHLCSRDDLHFIICGAGAGVPALRTQCEGLARVHFLPLQSAEDFPALLDCADIHLMPQRADAADLVLPSKLT
NMLASGKPVVATAAPDTELGKLVIQFGSLVEPGNSVAMADAIAALADDPPRRQALGSAGKAWAAAHLDRDAVLAEFERTL
LRLRAPRGVADQAAADLAGK

Sequences:

>Translated_420_residues
MKILLYCLNYAPELTGIGKYTGEQAEWLAARGHQVRVITAPPYYPAWKIDRAYRGLRYRREMRHAVDVMRAPLWVPERPS
GVKRLLHLASFAVTSLGWLARQVAWRPDVVFTVEPPLFCSPATLLFARLTGAKAWLHIQDYEVDAAFKLGLLKGGLPQRM
ASTFERFIMRRYDHVSSISHTMVALARQKGVDATRVSMLPNWVDLAALARRGDADFRARLGIPANAVVALYSGTIGTKQG
IEILADAARHLCSRDDLHFIICGAGAGVPALRTQCEGLARVHFLPLQSAEDFPALLDCADIHLMPQRADAADLVLPSKLT
NMLASGKPVVATAAPDTELGKLVIQFGSLVEPGNSVAMADAIAALADDPPRRQALGSAGKAWAAAHLDRDAVLAEFERTL
LRLRAPRGVADQAAADLAGK
>Mature_420_residues
MKILLYCLNYAPELTGIGKYTGEQAEWLAARGHQVRVITAPPYYPAWKIDRAYRGLRYRREMRHAVDVMRAPLWVPERPS
GVKRLLHLASFAVTSLGWLARQVAWRPDVVFTVEPPLFCSPATLLFARLTGAKAWLHIQDYEVDAAFKLGLLKGGLPQRM
ASTFERFIMRRYDHVSSISHTMVALARQKGVDATRVSMLPNWVDLAALARRGDADFRARLGIPANAVVALYSGTIGTKQG
IEILADAARHLCSRDDLHFIICGAGAGVPALRTQCEGLARVHFLPLQSAEDFPALLDCADIHLMPQRADAADLVLPSKLT
NMLASGKPVVATAAPDTELGKLVIQFGSLVEPGNSVAMADAIAALADDPPRRQALGSAGKAWAAAHLDRDAVLAEFERTL
LRLRAPRGVADQAAADLAGK

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788363, Length=413, Percent_Identity=47.4576271186441, Blast_Score=324, Evalue=6e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: NA

Molecular weight: Translated: 45760; Mature: 45760

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILLYCLNYAPELTGIGKYTGEQAEWLAARGHQVRVITAPPYYPAWKIDRAYRGLRYRR
CEEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHH
EMRHAVDVMRAPLWVPERPSGVKRLLHLASFAVTSLGWLARQVAWRPDVVFTVEPPLFCS
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCC
PATLLFARLTGAKAWLHIQDYEVDAAFKLGLLKGGLPQRMASTFERFIMRRYDHVSSISH
HHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TMVALARQKGVDATRVSMLPNWVDLAALARRGDADFRARLGIPANAVVALYSGTIGTKQG
HHHHHHHHCCCCHHHHHHCCCHHHHHHHHHCCCCCCEEECCCCCHHEEEEECCCCCCHHH
IEILADAARHLCSRDDLHFIICGAGAGVPALRTQCEGLARVHFLPLQSAEDFPALLDCAD
HHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHH
IHLMPQRADAADLVLPSKLTNMLASGKPVVATAAPDTELGKLVIQFGSLVEPGNSVAMAD
HEEECCCCCCHHEECHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHH
AIAALADDPPRRQALGSAGKAWAAAHLDRDAVLAEFERTLLRLRAPRGVADQAAADLAGK
HHHHHCCCCHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCC
>Mature Secondary Structure
MKILLYCLNYAPELTGIGKYTGEQAEWLAARGHQVRVITAPPYYPAWKIDRAYRGLRYRR
CEEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHH
EMRHAVDVMRAPLWVPERPSGVKRLLHLASFAVTSLGWLARQVAWRPDVVFTVEPPLFCS
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCC
PATLLFARLTGAKAWLHIQDYEVDAAFKLGLLKGGLPQRMASTFERFIMRRYDHVSSISH
HHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TMVALARQKGVDATRVSMLPNWVDLAALARRGDADFRARLGIPANAVVALYSGTIGTKQG
HHHHHHHHCCCCHHHHHHCCCHHHHHHHHHCCCCCCEEECCCCCHHEEEEECCCCCCHHH
IEILADAARHLCSRDDLHFIICGAGAGVPALRTQCEGLARVHFLPLQSAEDFPALLDCAD
HHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHH
IHLMPQRADAADLVLPSKLTNMLASGKPVVATAAPDTELGKLVIQFGSLVEPGNSVAMAD
HEEECCCCCCHHEECHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHH
AIAALADDPPRRQALGSAGKAWAAAHLDRDAVLAEFERTLLRLRAPRGVADQAAADLAGK
HHHHHCCCCHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7815923; 8759852; 9097040; 9278503 [H]