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The map label for this gene is wcaF [H]

Identifier: 94314774

GI number: 94314774

Start: 50095

End: 50715

Strand: Reverse

Name: wcaF [H]

Synonym: Rmet_5855

Alternate gene names: 94314774

Gene position: 50715-50095 (Counterclockwise)

Preceding gene: 94314775

Following gene: 94314773

Centisome position: 1.97

GC content: 65.06

Gene sequence:

>621_bases
ATGTTTCAGGACCTGTCGACTTTCCGCATGCCACCCGGCTTCCGTGGCCGCTCCGCCGTTGTCGTGCAACTGTGGTGGTG
TGTCCAGAGCCTGTTCTTTCATCACTCTCCGCATTTCGCCAACGGATTCCGCTGCTGGCTGTTGCGCCGCTTTGGCGCCC
GCGTGGGCCATCACGTCGTCATCCGGCCCAGCGTCCGCATCACCTATCCGTGGAAGGTCAGCATCGGCGACTACGCGTGG
ATCGGTGACGGCGCCGAGCTGTACAGCCTGGCCGAGATCGAGATCGGCAATCACGCGGTGGTATCCCAGTTCAGCTACCT
GTGCGGCGGCGACCATGACTACCGCAGCAGCGACTTCGCCATCCGGGGACGCCCTATCCGCATCGCGGACGAGGCGTGGG
TGGCCAGTCACACCTTTATCGCGCCGGGCGTCAGCATCGGCAAAGGGGCCGTAATCGGCGCCCGCAGCGCGGTATTCACC
GACATGCCTGCCGGCATGCTCTGCGTGGGCAGTCCTTGCCGGCCGTTGCGGCCGCGCCTGGACACCGCCTCGCAGAACCA
GACGCGTCCGCATTCGCAGGCGGCCGCGCCATCAGAACTAGATTCCGCTCAAGTCAACTAG

Upstream 100 bases:

>100_bases
GGTACGTACGCCAGCGCGGGGCCATGATGAAACCATCGTCACCGGCCTGACCGGGAACAAGGCTCGGGCTGGTTCCAGCC
CGGCATTAGGAGCCTGCTGT

Downstream 100 bases:

>100_bases
ATAACGGGAACGCGGAAACCAAACAGGGAACTCATGGCTGCCAATGATCGACATCTATCACAACATCCTATGGCCTAGGT
ACAAGGGAGCCGTATTCAGC

Product: putative acyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MFQDLSTFRMPPGFRGRSAVVVQLWWCVQSLFFHHSPHFANGFRCWLLRRFGARVGHHVVIRPSVRITYPWKVSIGDYAW
IGDGAELYSLAEIEIGNHAVVSQFSYLCGGDHDYRSSDFAIRGRPIRIADEAWVASHTFIAPGVSIGKGAVIGARSAVFT
DMPAGMLCVGSPCRPLRPRLDTASQNQTRPHSQAAAPSELDSAQVN

Sequences:

>Translated_206_residues
MFQDLSTFRMPPGFRGRSAVVVQLWWCVQSLFFHHSPHFANGFRCWLLRRFGARVGHHVVIRPSVRITYPWKVSIGDYAW
IGDGAELYSLAEIEIGNHAVVSQFSYLCGGDHDYRSSDFAIRGRPIRIADEAWVASHTFIAPGVSIGKGAVIGARSAVFT
DMPAGMLCVGSPCRPLRPRLDTASQNQTRPHSQAAAPSELDSAQVN
>Mature_206_residues
MFQDLSTFRMPPGFRGRSAVVVQLWWCVQSLFFHHSPHFANGFRCWLLRRFGARVGHHVVIRPSVRITYPWKVSIGDYAW
IGDGAELYSLAEIEIGNHAVVSQFSYLCGGDHDYRSSDFAIRGRPIRIADEAWVASHTFIAPGVSIGKGAVIGARSAVFT
DMPAGMLCVGSPCRPLRPRLDTASQNQTRPHSQAAAPSELDSAQVN

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1788367, Length=181, Percent_Identity=54.1436464088398, Blast_Score=216, Evalue=8e-58,
Organism=Escherichia coli, GI1786537, Length=148, Percent_Identity=28.3783783783784, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.- [C]

Molecular weight: Translated: 22782; Mature: 22782

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQDLSTFRMPPGFRGRSAVVVQLWWCVQSLFFHHSPHFANGFRCWLLRRFGARVGHHVV
CCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCEEE
IRPSVRITYPWKVSIGDYAWIGDGAELYSLAEIEIGNHAVVSQFSYLCGGDHDYRSSDFA
EECCEEEEEEEEECCCCEEEECCCHHEEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCEE
IRGRPIRIADEAWVASHTFIAPGVSIGKGAVIGARSAVFTDMPAGMLCVGSPCRPLRPRL
ECCCCEEEECCCEEECCEEECCCCCCCCCEEEECCCCEEECCCCCEEEECCCCCCCCCCC
DTASQNQTRPHSQAAAPSELDSAQVN
CCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFQDLSTFRMPPGFRGRSAVVVQLWWCVQSLFFHHSPHFANGFRCWLLRRFGARVGHHVV
CCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCEEE
IRPSVRITYPWKVSIGDYAWIGDGAELYSLAEIEIGNHAVVSQFSYLCGGDHDYRSSDFA
EECCEEEEEEEEECCCCEEEECCCHHEEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCEE
IRGRPIRIADEAWVASHTFIAPGVSIGKGAVIGARSAVFTDMPAGMLCVGSPCRPLRPRL
ECCCCEEEECCCEEECCEEECCCCCCCCCEEEECCCCEEECCCCCEEEECCCCCCCCCCC
DTASQNQTRPHSQAAAPSELDSAQVN
CCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]