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The map label for this gene is asfA [C]

Identifier: 94313260

GI number: 94313260

Start: 928213

End: 929862

Strand: Reverse

Name: asfA [C]

Synonym: Rmet_4335

Alternate gene names: 94313260

Gene position: 929862-928213 (Counterclockwise)

Preceding gene: 94313261

Following gene: 94313259

Centisome position: 36.04

GC content: 64.55

Gene sequence:

>1650_bases
ATGAATCAGGTCAAGCGCACGAAATCCTCGTCATCCACAGATACCGAGTTGGAAGCTGATGTCCTGGTGATAGGCGGCGG
TCCCGCAGGTACGTGGGCTGCACTGGAGGCCGTACGCAACGGCGCCACAAACGTCGTGCTGGCGGAGAAGGGATACTGCG
GCTCTTCCGGAGCGACGGCGGCGGCAGGAACCACGGTGTGGTACATCTCGCCGGACGAAGGTAAACGCGAGACCGCCATG
GACAGCCGCTACGACATGGGTGGTCGCCTGGCGGACTATCGTTGGATGGAACGCGTGCTGGACCGCACGTGGCATGGCAT
GAATGAGTTGGCCGATCACGGGTATCCATTCCCCCTCGATGCGACGGGCCGCTCACAGCGCGTGTCATTGCAGGGGCCCG
AGTATATGCGGCTGATGCGCAAACGCGTGGTGCAGGCGGGCGTGAAGATTCTGGACCAAAGCCCCGCGCTGGAACTGCTG
CAGGCTGACGATGGCAGCGTGGCTGGCGCGCGCGGTGTTCACCGCCAGAACGGCTCACAATGGCGAGTCAGGTCCAAAGC
GGTCGTCATCGCCACCGGTGGCTGCGCGTTCCTGAGTCGTGCGCTCGGCTGCAATGTCCTCACTGGTGACGGATTGCTGA
TGAGTGCGGAAGTGGGTGCCGAGATGTCTGGCATGGAGTTCTCCAACGCCTATGCCATCGCGCCCGAAAGCGGCTCGGTT
ACCAAGACCATGTTCTACAACTGGGCCTCTTTCACCGATGAAGCTGGCGAGGTGATTCCCGGCGCCGCATCCAAAGGGGG
GCGTAGCGTGATCGCGCGCGAGCTAAATCGGCAGAAGGTCTACGCCCGGTTGGACAAGGCCGATGAGGCGACACGTCTGG
CCATGCGCGCCTCGCAGCCCAACTTCTTCCTGCCGTTCGACCGTCAGGGCATCGATCCTTTCACACAACGCTTTTCCGTC
ACGCTGCGTCTCGAAGGCACGGTACGCGGAACCGGCGGGTTGCGTATCACCAGCGAAGACTGCGCCACCAGCGTGTCCGG
CCTGTATGCCGCCGGAGATGCTGCTACGCGCGAGCCCATCTGCGGCGGATTTACCGGAGGCGGCAGCCACAATGCAGCCT
GGGCGATTTCCTCCGGCTCATGGGCCGGCCAAGGGGCGGCGAGGTTTGCACTCCAGCGCGACACCAACCAGCGCTCGGCG
CGGGGCGCCGGTGTCGCGGCCATTTCTGGTGCCGGCCGCCAGACCATCAATCCTGCCGCGCTCACCCGAGCCGTGCAGGA
CGAGGTATTCCCATTCGACCGCAACTACTTTCGCCACGCCGACGTACTGGCACCTTCGCTCGGTCGTCTGGATGACGCGT
GGCTCGGCGTGCGCGAGTCCGCGCCTGCGGCGGTGGAGCAGATGCTTGCCGCGCGCGAGGCAGCGGCCATGCTTGCCACT
GCGCGCTGGATGTACGCCAGCGCCCTCGCCCGCAAGGAATCCCGCGGCATGCACAAGCGTTACGACCACTTGCAGCTGGA
TCCCACGCAGCACCACCATATCGTCAGCGGAGGATTGGACCAGGTCTGGGCCCGTCCGTTGGCCTATGACACTCAAGGTG
GTCCGCGATCGGCGCAAGCCAGGGCCGAGCAGGAAGGTATTGCCGCATGA

Upstream 100 bases:

>100_bases
TGCGCCAGTTTGCGCACTAACTAATGCCAAAAGCTTCATTCCCATGTCGGGATGTGTTGCCTAGCATTGCTGGTTGCTGC
ATGAAACCTTGGAGTGACAA

Downstream 100 bases:

>100_bases
TCGAACTCATTAGCGAAAGCCGCTGCACTGGCTGCAACATCTGCGTCCGCGTCTGCCCGGTCAACGTGTTCGATGCCGTG
CCTCAGGCCGCACCGACCAT

Product: fumarate reductase/succinate dehydrogenase flavoprotein-like protein

Products: FADH2; Fumarate [C]

Alternate protein names: FAD Dependent Oxidoreductase; Oxidoreductase; FAD-Dependent Pyridine Nucleotide-Disulphide Oxidoreductase; Oxidoreductase Putative L-Aspartate Oxidase; Glucose-Inhibited Division Protein A; Oxidoreductase Protein; Fumarate Reductase Flavoprotein Subunit; Fumarate/Succinate Reductase Flavoprotein Subunit; Oxidoreductase Flavoprotein AsfA; Pyridine Nucleotide-Disulfide Oxidoreductase

Number of amino acids: Translated: 549; Mature: 549

Protein sequence:

>549_residues
MNQVKRTKSSSSTDTELEADVLVIGGGPAGTWAALEAVRNGATNVVLAEKGYCGSSGATAAAGTTVWYISPDEGKRETAM
DSRYDMGGRLADYRWMERVLDRTWHGMNELADHGYPFPLDATGRSQRVSLQGPEYMRLMRKRVVQAGVKILDQSPALELL
QADDGSVAGARGVHRQNGSQWRVRSKAVVIATGGCAFLSRALGCNVLTGDGLLMSAEVGAEMSGMEFSNAYAIAPESGSV
TKTMFYNWASFTDEAGEVIPGAASKGGRSVIARELNRQKVYARLDKADEATRLAMRASQPNFFLPFDRQGIDPFTQRFSV
TLRLEGTVRGTGGLRITSEDCATSVSGLYAAGDAATREPICGGFTGGGSHNAAWAISSGSWAGQGAARFALQRDTNQRSA
RGAGVAAISGAGRQTINPAALTRAVQDEVFPFDRNYFRHADVLAPSLGRLDDAWLGVRESAPAAVEQMLAAREAAAMLAT
ARWMYASALARKESRGMHKRYDHLQLDPTQHHHIVSGGLDQVWARPLAYDTQGGPRSAQARAEQEGIAA

Sequences:

>Translated_549_residues
MNQVKRTKSSSSTDTELEADVLVIGGGPAGTWAALEAVRNGATNVVLAEKGYCGSSGATAAAGTTVWYISPDEGKRETAM
DSRYDMGGRLADYRWMERVLDRTWHGMNELADHGYPFPLDATGRSQRVSLQGPEYMRLMRKRVVQAGVKILDQSPALELL
QADDGSVAGARGVHRQNGSQWRVRSKAVVIATGGCAFLSRALGCNVLTGDGLLMSAEVGAEMSGMEFSNAYAIAPESGSV
TKTMFYNWASFTDEAGEVIPGAASKGGRSVIARELNRQKVYARLDKADEATRLAMRASQPNFFLPFDRQGIDPFTQRFSV
TLRLEGTVRGTGGLRITSEDCATSVSGLYAAGDAATREPICGGFTGGGSHNAAWAISSGSWAGQGAARFALQRDTNQRSA
RGAGVAAISGAGRQTINPAALTRAVQDEVFPFDRNYFRHADVLAPSLGRLDDAWLGVRESAPAAVEQMLAAREAAAMLAT
ARWMYASALARKESRGMHKRYDHLQLDPTQHHHIVSGGLDQVWARPLAYDTQGGPRSAQARAEQEGIAA
>Mature_549_residues
MNQVKRTKSSSSTDTELEADVLVIGGGPAGTWAALEAVRNGATNVVLAEKGYCGSSGATAAAGTTVWYISPDEGKRETAM
DSRYDMGGRLADYRWMERVLDRTWHGMNELADHGYPFPLDATGRSQRVSLQGPEYMRLMRKRVVQAGVKILDQSPALELL
QADDGSVAGARGVHRQNGSQWRVRSKAVVIATGGCAFLSRALGCNVLTGDGLLMSAEVGAEMSGMEFSNAYAIAPESGSV
TKTMFYNWASFTDEAGEVIPGAASKGGRSVIARELNRQKVYARLDKADEATRLAMRASQPNFFLPFDRQGIDPFTQRFSV
TLRLEGTVRGTGGLRITSEDCATSVSGLYAAGDAATREPICGGFTGGGSHNAAWAISSGSWAGQGAARFALQRDTNQRSA
RGAGVAAISGAGRQTINPAALTRAVQDEVFPFDRNYFRHADVLAPSLGRLDDAWLGVRESAPAAVEQMLAAREAAAMLAT
ARWMYASALARKESRGMHKRYDHLQLDPTQHHHIVSGGLDQVWARPLAYDTQGGPRSAQARAEQEGIAA

Specific function: Two Distinct, Membrane-Bound, FAD-Containing Enzymes Are Responsible For The Catalysis Of Fumarate And Succinate Interconversion; The Fumarate Reductase Is Used In Anaerobic Growth, And The Succinate Dehydrogenase Is Used In Aerobic Growth. [C]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.3.99.1 [C]

Molecular weight: Translated: 58757; Mature: 58757

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQVKRTKSSSSTDTELEADVLVIGGGPAGTWAALEAVRNGATNVVLAEKGYCGSSGATA
CCCHHCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCC
AAGTTVWYISPDEGKRETAMDSRYDMGGRLADYRWMERVLDRTWHGMNELADHGYPFPLD
CCCCEEEEECCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCC
ATGRSQRVSLQGPEYMRLMRKRVVQAGVKILDQSPALELLQADDGSVAGARGVHRQNGSQ
CCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCC
WRVRSKAVVIATGGCAFLSRALGCNVLTGDGLLMSAEVGAEMSGMEFSNAYAIAPESGSV
EEEECCEEEEEECCHHHHHHHHCCEEEECCCEEEECCCCCCCCCCEECCEEEECCCCCCE
TKTMFYNWASFTDEAGEVIPGAASKGGRSVIARELNRQKVYARLDKADEATRLAMRASQP
EEEEEEEHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCC
NFFLPFDRQGIDPFTQRFSVTLRLEGTVRGTGGLRITSEDCATSVSGLYAAGDAATREPI
CEEECCCCCCCCHHHHCEEEEEEEEEEEECCCCEEEECHHHHHHHHCCEECCCCCCCCCC
CGGFTGGGSHNAAWAISSGSWAGQGAARFALQRDTNQRSARGAGVAAISGAGRQTINPAA
CCCCCCCCCCCEEEEEECCCCCCCCHHHEEECCCCCCHHCCCCCEEEECCCCCCCCCHHH
LTRAVQDEVFPFDRNYFRHADVLAPSLGRLDDAWLGVRESAPAAVEQMLAAREAAAMLAT
HHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
ARWMYASALARKESRGMHKRYDHLQLDPTQHHHIVSGGLDQVWARPLAYDTQGGPRSAQA
HHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCEECCCCHHHHHCCCCCCCCCCCCCCHHH
RAEQEGIAA
HHHHCCCCC
>Mature Secondary Structure
MNQVKRTKSSSSTDTELEADVLVIGGGPAGTWAALEAVRNGATNVVLAEKGYCGSSGATA
CCCHHCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCC
AAGTTVWYISPDEGKRETAMDSRYDMGGRLADYRWMERVLDRTWHGMNELADHGYPFPLD
CCCCEEEEECCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCC
ATGRSQRVSLQGPEYMRLMRKRVVQAGVKILDQSPALELLQADDGSVAGARGVHRQNGSQ
CCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCC
WRVRSKAVVIATGGCAFLSRALGCNVLTGDGLLMSAEVGAEMSGMEFSNAYAIAPESGSV
EEEECCEEEEEECCHHHHHHHHCCEEEECCCEEEECCCCCCCCCCEECCEEEECCCCCCE
TKTMFYNWASFTDEAGEVIPGAASKGGRSVIARELNRQKVYARLDKADEATRLAMRASQP
EEEEEEEHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCC
NFFLPFDRQGIDPFTQRFSVTLRLEGTVRGTGGLRITSEDCATSVSGLYAAGDAATREPI
CEEECCCCCCCCHHHHCEEEEEEEEEEEECCCCEEEECHHHHHHHHCCEECCCCCCCCCC
CGGFTGGGSHNAAWAISSGSWAGQGAARFALQRDTNQRSARGAGVAAISGAGRQTINPAA
CCCCCCCCCCCEEEEEECCCCCCCCHHHEEECCCCCCHHCCCCCEEEECCCCCCCCCHHH
LTRAVQDEVFPFDRNYFRHADVLAPSLGRLDDAWLGVRESAPAAVEQMLAAREAAAMLAT
HHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
ARWMYASALARKESRGMHKRYDHLQLDPTQHHHIVSGGLDQVWARPLAYDTQGGPRSAQA
HHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCEECCCCHHHHHCCCCCCCCCCCCCCHHH
RAEQEGIAA
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: ATP; Dithiothreitol; FAD; Fe-S-clusters [C]

Metal ions: Anions; Diphosphate; Fe [C]

Kcat value (1/min): 10000-13000 [C]

Specific activity: 509

Km value (mM): 0.4 {fumarate}} 0.02 {succinate}} 0.3 {ferricyanide}} [C]

Substrates: FAD; Succinate [C]

Specific reaction: FAD + Succinate --> FADH2 + Fumarate [C]

General reaction: Redox reaction [C]

Inhibitor: 5, 5'-Dithiobis (2-nitrobenzoate); Chaotropic reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA