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The map label for this gene is bioI [H]

Identifier: 94312750

GI number: 94312750

Start: 362839

End: 363990

Strand: Direct

Name: bioI [H]

Synonym: Rmet_3820

Alternate gene names: 94312750

Gene position: 362839-363990 (Clockwise)

Preceding gene: 94312749

Following gene: 94312751

Centisome position: 14.06

GC content: 67.45

Gene sequence:

>1152_bases
ATGCCCCCCAACGCTTCCCTTGCCCCCGCCGATCCGATTGCAGCCGTCACGCACGCCGATCCTTATCCTTACTATCGTGA
CCTCGCGAGCACTCGCCCGTTCTACTTCGATCCAGCACTGAAGCTGTGGGTCGCCGCCAGCGCGGCGGCCGTCGCGGAAG
TGCTGGACCATGCCGATGGCCGCGTGCGGCCAGCCGCACAGCCCGTTCCGCCGTCGCTCGCCGGATCTGCCGCAGGTGAC
CTGTTCGGGCGGCTGATCCGTATGAATGACGGCGTGGCCCATGCCCCGCTGAAGGCAATCGTGGCGCGGCAGATGGCGGC
GCTGGATCTGTCCGATGCACGCAGTCGGGCCCGCGCGCTGGCAGCGGCATTGCCCCGCGTGGACCACGACGACGCACACG
AAGCCAATCGCTGGATGTTCACGTTGCCGGTGCTCGCCATCGCCGATCTGCTGGGCCTTCCGCTTGAGCGCGACACGGAC
GGTCACGTCTCGACGCGGGTGGCCGGCTTTGTTGCCACCTTTGCCGCGGCCATGTCACCGCTGGCATCCCCGGCGGATGT
CGAGGCTGGCGTTGCTGCCGCGCAATGGCTGACGCGCCTGGCGCAAACCGCCGAAGATCGCGAAACGGCAGACATCGGCG
TGCTGGCCGCGCTGGCGCAGGCGGGGGGTGACAGCAAGATGGATCCGGCCACGCTGACGGCAAACGCGATCGGCCTGATG
ATCCAGTCGTGCGAAGCGACTGCCGGGCTGATCGGCAACACGCTCGTTTATCTCGGCAGATCTGACTGGCAAGGGGCCGA
TATCGGAGCGAGCGTAGATCGGGTGGCAAGGATCGATCCACCCGTGCAGAACACGCGCCGTTTCATGGCAGCGGATGCGA
TGTTGTGCGGCCAGCAGGTCAAGGAGGGCGATGCGGTGCTGGTATTGCTGGCCGCGGCGGCGCATGATCCTTCCTCCGCC
GCGGAGGAGCGCGCGTGGACCTTTGGTGCGGGGCGGCACGGTTGCCCAGGCGATGCGCTGGCTCGCGTGCTGGCCACGTG
CACGGTCGATGCGCTGCTCGCGCGCGACGTACACCCGGACCGGCTTGCTGCCGGCGTGCGCTACCGGCCATCCGTCAATG
CGCGCATTCCCAGCTTTTCATCCAACAACTAA

Upstream 100 bases:

>100_bases
GGTGGTGCCTTAGTGGTGCAATCGACATTTCGCGTTGTCGTGAAACATCCAGTGCGATGGCGAACCATACTGGGTGGCAT
CTACCAGATGCGAGCAGGTC

Downstream 100 bases:

>100_bases
ATGGAGCCCCCAGCCATGATCGCCATCATCTTTGAAGTCGAGCCCGCGCCAGGGCGCCAGGACGCCTACCTCGACATCGC
CGCGCATCTGAAATCGACGC

Product: putative cytochrome p450 oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MPPNASLAPADPIAAVTHADPYPYYRDLASTRPFYFDPALKLWVAASAAAVAEVLDHADGRVRPAAQPVPPSLAGSAAGD
LFGRLIRMNDGVAHAPLKAIVARQMAALDLSDARSRARALAAALPRVDHDDAHEANRWMFTLPVLAIADLLGLPLERDTD
GHVSTRVAGFVATFAAAMSPLASPADVEAGVAAAQWLTRLAQTAEDRETADIGVLAALAQAGGDSKMDPATLTANAIGLM
IQSCEATAGLIGNTLVYLGRSDWQGADIGASVDRVARIDPPVQNTRRFMAADAMLCGQQVKEGDAVLVLLAAAAHDPSSA
AEERAWTFGAGRHGCPGDALARVLATCTVDALLARDVHPDRLAAGVRYRPSVNARIPSFSSNN

Sequences:

>Translated_383_residues
MPPNASLAPADPIAAVTHADPYPYYRDLASTRPFYFDPALKLWVAASAAAVAEVLDHADGRVRPAAQPVPPSLAGSAAGD
LFGRLIRMNDGVAHAPLKAIVARQMAALDLSDARSRARALAAALPRVDHDDAHEANRWMFTLPVLAIADLLGLPLERDTD
GHVSTRVAGFVATFAAAMSPLASPADVEAGVAAAQWLTRLAQTAEDRETADIGVLAALAQAGGDSKMDPATLTANAIGLM
IQSCEATAGLIGNTLVYLGRSDWQGADIGASVDRVARIDPPVQNTRRFMAADAMLCGQQVKEGDAVLVLLAAAAHDPSSA
AEERAWTFGAGRHGCPGDALARVLATCTVDALLARDVHPDRLAAGVRYRPSVNARIPSFSSNN
>Mature_382_residues
PPNASLAPADPIAAVTHADPYPYYRDLASTRPFYFDPALKLWVAASAAAVAEVLDHADGRVRPAAQPVPPSLAGSAAGDL
FGRLIRMNDGVAHAPLKAIVARQMAALDLSDARSRARALAAALPRVDHDDAHEANRWMFTLPVLAIADLLGLPLERDTDG
HVSTRVAGFVATFAAAMSPLASPADVEAGVAAAQWLTRLAQTAEDRETADIGVLAALAQAGGDSKMDPATLTANAIGLMI
QSCEATAGLIGNTLVYLGRSDWQGADIGASVDRVARIDPPVQNTRRFMAADAMLCGQQVKEGDAVLVLLAAAAHDPSSAA
EERAWTFGAGRHGCPGDALARVLATCTVDALLARDVHPDRLAAGVRYRPSVNARIPSFSSNN

Specific function: Catalyzes the C-C bond cleavage of fatty acid linked to acyl carrier protein (ACP) to generate pimelic acid for biotin biosynthesis. It has high affinity for long-chain fatty acids with the greatest affinity for myristic acid [H]

COG id: COG2124

COG function: function code Q; Cytochrome P450

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome P450 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001128
- InterPro:   IPR002397
- InterPro:   IPR017972 [H]

Pfam domain/function: PF00067 p450 [H]

EC number: NA

Molecular weight: Translated: 39966; Mature: 39834

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS00086 CYTOCHROME_P450

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPNASLAPADPIAAVTHADPYPYYRDLASTRPFYFDPALKLWVAASAAAVAEVLDHADG
CCCCCCCCCCCCCCEECCCCCCHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHCCCC
RVRPAAQPVPPSLAGSAAGDLFGRLIRMNDGVAHAPLKAIVARQMAALDLSDARSRARAL
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHH
AAALPRVDHDDAHEANRWMFTLPVLAIADLLGLPLERDTDGHVSTRVAGFVATFAAAMSP
HHHHCCCCCCCCHHHCCEEEEHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
LASPADVEAGVAAAQWLTRLAQTAEDRETADIGVLAALAQAGGDSKMDPATLTANAIGLM
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
IQSCEATAGLIGNTLVYLGRSDWQGADIGASVDRVARIDPPVQNTRRFMAADAMLCGQQV
HHHHHHHHHHHHCEEEEECCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
KEGDAVLVLLAAAAHDPSSAAEERAWTFGAGRHGCPGDALARVLATCTVDALLARDVHPD
CCCCEEEEEEEHHCCCCCHHHHHHCEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHH
RLAAGVRYRPSVNARIPSFSSNN
HHHCCCEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
PPNASLAPADPIAAVTHADPYPYYRDLASTRPFYFDPALKLWVAASAAAVAEVLDHADG
CCCCCCCCCCCCCEECCCCCCHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHCCCC
RVRPAAQPVPPSLAGSAAGDLFGRLIRMNDGVAHAPLKAIVARQMAALDLSDARSRARAL
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHH
AAALPRVDHDDAHEANRWMFTLPVLAIADLLGLPLERDTDGHVSTRVAGFVATFAAAMSP
HHHHCCCCCCCCHHHCCEEEEHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
LASPADVEAGVAAAQWLTRLAQTAEDRETADIGVLAALAQAGGDSKMDPATLTANAIGLM
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
IQSCEATAGLIGNTLVYLGRSDWQGADIGASVDRVARIDPPVQNTRRFMAADAMLCGQQV
HHHHHHHHHHHHCEEEEECCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
KEGDAVLVLLAAAAHDPSSAAEERAWTFGAGRHGCPGDALARVLATCTVDALLARDVHPD
CCCCEEEEEEEHHCCCCCHHHHHHCEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHH
RLAAGVRYRPSVNARIPSFSSNN
HHHCCCEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8763940; 9387221; 9384377 [H]