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The map label for this gene is cysK [H]

Identifier: 94312749

GI number: 94312749

Start: 361745

End: 362719

Strand: Direct

Name: cysK [H]

Synonym: Rmet_3819

Alternate gene names: 94312749

Gene position: 361745-362719 (Clockwise)

Preceding gene: 94312748

Following gene: 94312750

Centisome position: 14.02

GC content: 64.51

Gene sequence:

>975_bases
ATGGCAACGCGGAACGGTTTTATCGGCGCAGTCGGCAACACGCCGTTGATCAGGCTCGGCAAGCTCAGCGCGGAAACGGG
CTGCGAGATCCTCGGCAAGGCCGAGTTCCTGAACCCGGGTGGCTCGGTCAAGGACCGCGCCGCCTTGTACATCATTGCTG
ACGCCGAGCGGCGCGGCACGCTGAAGCCTGGCGGCACCGTGGTGGAAGGCACGGCCGGCAACACCGGCATCGGCCTGGCG
CATATCTGCGCCGCGCGTGGCTATCGCTGCGTGATCGTGATTCCGGAGACCCAGTCGAAAGAGAAGATGGACTTGCTGCG
CGTGCTCGGTGCCGAAGTACGCACCGTGCCGGCGGTACCCTACGCCGACCCCAATAACTATCAGAAGGTGGCAGGGCGCC
TGGCGCAGGAACTCGACAACGCGATCTGGGCGAACCAGTTCGACAACACGGTCAACCGGCAGGCCCACTACGAGACCACG
GGGCCGGAGATCTGGCGAGATACCGACGGCAAGATCGATGCTTTCGTCTGCGCCACTGGCACAGGCGGGACGCTCGCGGG
CGTTGCCCGCTTTCTCAAGGCGCAGAACCCGGCGGTGCGAACGGTGCTGGCCGATCCACATGGCAGTGGTCTGTACAGCT
TCGTGAAGACCGGGCAGATCCGGGCCGAAGGTAATTCGATTACCGAGGGGATCGGGTCAAGCCGCATCACTGCGAATCTC
GCGGACACACCAATCGACGATGCGGTGCGCATCGATGACCAGCGTTGCGTGACGATGGTCTACCGGCTGCTGCGCGAGGA
AGGGCTGTACGTGGGTGGGTCGACGGGCATCAATGTGGCGGCCGCGGTGCAGATCGCGCAGGATCTGGGGCCGGGTCACA
CCATCGTGACGCTACTGTGCGATCGTGGCAGCTTGTATTCGGCGCGGCTGTTCAACGCCGAATGGCTGGCGCAAAAGGGG
CTGGTGGCTGGCTAG

Upstream 100 bases:

>100_bases
CCGGACGATGCTTCCGGCCCGCAATCCGCGCCATCCGTATGCCCGGACTAAGCTGTAGCTGTAGGAATGAGCGGCACAGT
TCGGGCATGCGGGAGGTGCG

Downstream 100 bases:

>100_bases
CCGCGCTTGGTGGTGCCTCGGTGGTGCCTTAGTGGTGCAATCGACATTTCGCGTTGTCGTGAAACATCCAGTGCGATGGC
GAACCATACTGGGTGGCATC

Product: cysteine synthase A

Products: NA

Alternate protein names: Beta-thionase; Serine sulfhydrase [H]

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MATRNGFIGAVGNTPLIRLGKLSAETGCEILGKAEFLNPGGSVKDRAALYIIADAERRGTLKPGGTVVEGTAGNTGIGLA
HICAARGYRCVIVIPETQSKEKMDLLRVLGAEVRTVPAVPYADPNNYQKVAGRLAQELDNAIWANQFDNTVNRQAHYETT
GPEIWRDTDGKIDAFVCATGTGGTLAGVARFLKAQNPAVRTVLADPHGSGLYSFVKTGQIRAEGNSITEGIGSSRITANL
ADTPIDDAVRIDDQRCVTMVYRLLREEGLYVGGSTGINVAAAVQIAQDLGPGHTIVTLLCDRGSLYSARLFNAEWLAQKG
LVAG

Sequences:

>Translated_324_residues
MATRNGFIGAVGNTPLIRLGKLSAETGCEILGKAEFLNPGGSVKDRAALYIIADAERRGTLKPGGTVVEGTAGNTGIGLA
HICAARGYRCVIVIPETQSKEKMDLLRVLGAEVRTVPAVPYADPNNYQKVAGRLAQELDNAIWANQFDNTVNRQAHYETT
GPEIWRDTDGKIDAFVCATGTGGTLAGVARFLKAQNPAVRTVLADPHGSGLYSFVKTGQIRAEGNSITEGIGSSRITANL
ADTPIDDAVRIDDQRCVTMVYRLLREEGLYVGGSTGINVAAAVQIAQDLGPGHTIVTLLCDRGSLYSARLFNAEWLAQKG
LVAG
>Mature_323_residues
ATRNGFIGAVGNTPLIRLGKLSAETGCEILGKAEFLNPGGSVKDRAALYIIADAERRGTLKPGGTVVEGTAGNTGIGLAH
ICAARGYRCVIVIPETQSKEKMDLLRVLGAEVRTVPAVPYADPNNYQKVAGRLAQELDNAIWANQFDNTVNRQAHYETTG
PEIWRDTDGKIDAFVCATGTGGTLAGVARFLKAQNPAVRTVLADPHGSGLYSFVKTGQIRAEGNSITEGIGSSRITANLA
DTPIDDAVRIDDQRCVTMVYRLLREEGLYVGGSTGINVAAAVQIAQDLGPGHTIVTLLCDRGSLYSARLFNAEWLAQKGL
VAG

Specific function: Two Cysteine Synthase Enzymes Are Found. Both Catalyze The Same Reaction. Cysteine Synthase B Can Also Use Thiosulfate In Place Of Sulfide To Give Cysteine Thiosulfonate As A Product. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=319, Percent_Identity=43.8871473354232, Blast_Score=255, Evalue=5e-68,
Organism=Homo sapiens, GI295821200, Length=319, Percent_Identity=43.8871473354232, Blast_Score=255, Evalue=5e-68,
Organism=Homo sapiens, GI4557415, Length=319, Percent_Identity=43.8871473354232, Blast_Score=255, Evalue=5e-68,
Organism=Escherichia coli, GI2367138, Length=313, Percent_Identity=37.0607028753994, Blast_Score=183, Evalue=1e-47,
Organism=Escherichia coli, GI1788754, Length=314, Percent_Identity=37.2611464968153, Blast_Score=175, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17562970, Length=310, Percent_Identity=39.3548387096774, Blast_Score=194, Evalue=5e-50,
Organism=Caenorhabditis elegans, GI17535051, Length=311, Percent_Identity=38.5852090032154, Blast_Score=192, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI32566674, Length=279, Percent_Identity=41.2186379928315, Blast_Score=192, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI115535073, Length=316, Percent_Identity=40.5063291139241, Blast_Score=191, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI17534315, Length=329, Percent_Identity=37.0820668693009, Blast_Score=172, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI25147552, Length=324, Percent_Identity=35.4938271604938, Blast_Score=164, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI17561720, Length=309, Percent_Identity=37.2168284789644, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI32566672, Length=226, Percent_Identity=34.9557522123894, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71996324, Length=323, Percent_Identity=27.5541795665635, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17539640, Length=341, Percent_Identity=27.2727272727273, Blast_Score=75, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6321449, Length=331, Percent_Identity=42.9003021148036, Blast_Score=231, Evalue=9e-62,
Organism=Saccharomyces cerevisiae, GI6321594, Length=331, Percent_Identity=37.4622356495468, Blast_Score=199, Evalue=6e-52,
Organism=Drosophila melanogaster, GI24643623, Length=318, Percent_Identity=39.937106918239, Blast_Score=210, Evalue=1e-54,
Organism=Drosophila melanogaster, GI20129101, Length=318, Percent_Identity=39.937106918239, Blast_Score=210, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR005857
- InterPro:   IPR000644
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00571 CBS; PF00291 PALP [H]

EC number: =4.2.1.22 [H]

Molecular weight: Translated: 34297; Mature: 34166

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATRNGFIGAVGNTPLIRLGKLSAETGCEILGKAEFLNPGGSVKDRAALYIIADAERRGT
CCCCCCCEEECCCCCEEEEECCCCCCCCCEECCCEEECCCCCCCCCEEEEEEEECCCCCC
LKPGGTVVEGTAGNTGIGLAHICAARGYRCVIVIPETQSKEKMDLLRVLGAEVRTVPAVP
CCCCCEEEECCCCCCCCHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCHHHCCCCCC
YADPNNYQKVAGRLAQELDNAIWANQFDNTVNRQAHYETTGPEIWRDTDGKIDAFVCATG
CCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHCCCCEECCCCCCCCCCCCCCEEEEEEECC
TGGTLAGVARFLKAQNPAVRTVLADPHGSGLYSFVKTGQIRAEGNSITEGIGSSRITANL
CCHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHCCEEEECCCHHHHCCCCCEEEEEC
ADTPIDDAVRIDDQRCVTMVYRLLREEGLYVGGSTGINVAAAVQIAQDLGPGHTIVTLLC
CCCCCCCCEEECCHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHCCCCCEEEEEEE
DRGSLYSARLFNAEWLAQKGLVAG
CCCCEEEEEECCHHHHHHCCCCCC
>Mature Secondary Structure 
ATRNGFIGAVGNTPLIRLGKLSAETGCEILGKAEFLNPGGSVKDRAALYIIADAERRGT
CCCCCCEEECCCCCEEEEECCCCCCCCCEECCCEEECCCCCCCCCEEEEEEEECCCCCC
LKPGGTVVEGTAGNTGIGLAHICAARGYRCVIVIPETQSKEKMDLLRVLGAEVRTVPAVP
CCCCCEEEECCCCCCCCHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCHHHCCCCCC
YADPNNYQKVAGRLAQELDNAIWANQFDNTVNRQAHYETTGPEIWRDTDGKIDAFVCATG
CCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHCCCCEECCCCCCCCCCCCCCEEEEEEECC
TGGTLAGVARFLKAQNPAVRTVLADPHGSGLYSFVKTGQIRAEGNSITEGIGSSRITANL
CCHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHCCEEEECCCHHHHCCCCCEEEEEC
ADTPIDDAVRIDDQRCVTMVYRLLREEGLYVGGSTGINVAAAVQIAQDLGPGHTIVTLLC
CCCCCCCCEEECCHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHCCCCCEEEEEEE
DRGSLYSARLFNAEWLAQKGLVAG
CCCCEEEEEECCHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]