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The map label for this gene is ynfL [H]

Identifier: 94312723

GI number: 94312723

Start: 332106

End: 333020

Strand: Direct

Name: ynfL [H]

Synonym: Rmet_3793

Alternate gene names: 94312723

Gene position: 332106-333020 (Clockwise)

Preceding gene: 94312719

Following gene: 291481494

Centisome position: 12.87

GC content: 59.67

Gene sequence:

>915_bases
ATGGATCTCAGGCAACTACGCTATTTCGTCGCGCTGGCAGAGGAGTTGCATTTCGGGCGTGCGGCAGCCCGACTGGCCAT
TACCCAGCCCCCGCTGAGTTTCAATATTGCGAAACTGGAGGCATCACTAGGGGTGCAATTGCTGCAACGCACTACCCGAG
AGGTAGCGCTGACTGCGGCGGGCAAAGAGATCTACAAGGAGGCGCTGAAGATCCTGGCGCTAACGGCGAATGCACGTGAA
CTGGCCGGGCGCGCAGCGCGTGGCGAGATTGGTGCAGTACATGTGGGTTTCGTTGGGTCCGCGGTCCTCACCCCATTGGG
GGAGCGCATCCGAGCCTTCGGAGCCATTCACCCGGACGTAGATGTCGTGCTGCACGAGCTGAATAGCTTCGAGCAGATCG
ACGCGCTGCAGGCCAGGCATATTGACATCGGCATTATTCATCCGCGCGTGATCCCTAACGGGATTGCCTCCCACTCCCTG
CACGGCGAGCACTTCATTTGCCTGCTGCCCGCGGATCATCCGCTGGCGCGCGACAGGACCATTGATGTCCGCGAGCTGCG
ACACGACGACTTCGTCCTGTTTCCGCGACATTTCTCGCCAGAGTTCCATGACCAGATTGTTGCGCTGTGCGCGAGTGTCG
GATTTACACCAAATATCCGCCACCAAGTCCGTCATATGCTGACCATTGCCACGCTGGTCGCGAAGCGCTTTGGTGTGTCG
ATTGTGCCGCGGTCGGTAGATGTGGTTACTTTGCCTAATCTGGCGTGCCGCCCCTTGGGAAAGCAAGCGACTCCGTCGGA
ACTGGTCGGGATCTGGCGCGACGACGAGCAAGGATCAGCCGTGCTTTCGATGTTGCATGCACTTGGCGCGCCAGTACAAC
AACGCGAGCTTGCAAACTCGCCGCGTCTCGCCTGA

Upstream 100 bases:

>100_bases
GCCGACGCGCTTGTGGCCATTCTTGGCTCCGAGGTCACCAGTGTCCAATACAATTATTGGGCTCATTCATACACGGCTTG
AATCAATCCCCGAGGCTTCC

Downstream 100 bases:

>100_bases
CGGATACCTGCTCAGCCGCGGGGCGGCTTTGCCCGTCGGGTGGGCGCAGACGGCCTGACCAAAAGGCCGTTCAGAATGAG
TTCGTAGAATTCGCTGAAGG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MDLRQLRYFVALAEELHFGRAAARLAITQPPLSFNIAKLEASLGVQLLQRTTREVALTAAGKEIYKEALKILALTANARE
LAGRAARGEIGAVHVGFVGSAVLTPLGERIRAFGAIHPDVDVVLHELNSFEQIDALQARHIDIGIIHPRVIPNGIASHSL
HGEHFICLLPADHPLARDRTIDVRELRHDDFVLFPRHFSPEFHDQIVALCASVGFTPNIRHQVRHMLTIATLVAKRFGVS
IVPRSVDVVTLPNLACRPLGKQATPSELVGIWRDDEQGSAVLSMLHALGAPVQQRELANSPRLA

Sequences:

>Translated_304_residues
MDLRQLRYFVALAEELHFGRAAARLAITQPPLSFNIAKLEASLGVQLLQRTTREVALTAAGKEIYKEALKILALTANARE
LAGRAARGEIGAVHVGFVGSAVLTPLGERIRAFGAIHPDVDVVLHELNSFEQIDALQARHIDIGIIHPRVIPNGIASHSL
HGEHFICLLPADHPLARDRTIDVRELRHDDFVLFPRHFSPEFHDQIVALCASVGFTPNIRHQVRHMLTIATLVAKRFGVS
IVPRSVDVVTLPNLACRPLGKQATPSELVGIWRDDEQGSAVLSMLHALGAPVQQRELANSPRLA
>Mature_304_residues
MDLRQLRYFVALAEELHFGRAAARLAITQPPLSFNIAKLEASLGVQLLQRTTREVALTAAGKEIYKEALKILALTANARE
LAGRAARGEIGAVHVGFVGSAVLTPLGERIRAFGAIHPDVDVVLHELNSFEQIDALQARHIDIGIIHPRVIPNGIASHSL
HGEHFICLLPADHPLARDRTIDVRELRHDDFVLFPRHFSPEFHDQIVALCASVGFTPNIRHQVRHMLTIATLVAKRFGVS
IVPRSVDVVTLPNLACRPLGKQATPSELVGIWRDDEQGSAVLSMLHALGAPVQQRELANSPRLA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=293, Percent_Identity=37.542662116041, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI2367136, Length=288, Percent_Identity=35.4166666666667, Blast_Score=166, Evalue=1e-42,
Organism=Escherichia coli, GI1788887, Length=296, Percent_Identity=33.445945945946, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1790399, Length=266, Percent_Identity=27.4436090225564, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI145693105, Length=246, Percent_Identity=29.6747967479675, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788748, Length=245, Percent_Identity=29.3877551020408, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1788297, Length=249, Percent_Identity=27.3092369477912, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI157672245, Length=250, Percent_Identity=28, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789173, Length=193, Percent_Identity=31.6062176165803, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787530, Length=268, Percent_Identity=25.3731343283582, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1790208, Length=172, Percent_Identity=29.6511627906977, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33337; Mature: 33337

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLRQLRYFVALAEELHFGRAAARLAITQPPLSFNIAKLEASLGVQLLQRTTREVALTAA
CCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHH
GKEIYKEALKILALTANARELAGRAARGEIGAVHVGFVGSAVLTPLGERIRAFGAIHPDV
HHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCH
DVVLHELNSFEQIDALQARHIDIGIIHPRVIPNGIASHSLHGEHFICLLPADHPLARDRT
HHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHCCCCCCEEEEEECCCCCCCCCCC
IDVRELRHDDFVLFPRHFSPEFHDQIVALCASVGFTPNIRHQVRHMLTIATLVAKRFGVS
CHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCE
IVPRSVDVVTLPNLACRPLGKQATPSELVGIWRDDEQGSAVLSMLHALGAPVQQRELANS
ECCCCEEEEECCCCCCCCCCCCCCHHHHEEEECCCCCHHHHHHHHHHHCCCHHHHHHCCC
PRLA
CCCC
>Mature Secondary Structure
MDLRQLRYFVALAEELHFGRAAARLAITQPPLSFNIAKLEASLGVQLLQRTTREVALTAA
CCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHH
GKEIYKEALKILALTANARELAGRAARGEIGAVHVGFVGSAVLTPLGERIRAFGAIHPDV
HHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCH
DVVLHELNSFEQIDALQARHIDIGIIHPRVIPNGIASHSLHGEHFICLLPADHPLARDRT
HHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHCCCCCCEEEEEECCCCCCCCCCC
IDVRELRHDDFVLFPRHFSPEFHDQIVALCASVGFTPNIRHQVRHMLTIATLVAKRFGVS
CHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCE
IVPRSVDVVTLPNLACRPLGKQATPSELVGIWRDDEQGSAVLSMLHALGAPVQQRELANS
ECCCCEEEEECCCCCCCCCCCCCCHHHHEEEECCCCCHHHHHHHHHHHCCCHHHHHHCCC
PRLA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]