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The map label for this gene is entE [H]

Identifier: 94312707

GI number: 94312707

Start: 313135

End: 314838

Strand: Direct

Name: entE [H]

Synonym: Rmet_3777

Alternate gene names: 94312707

Gene position: 313135-314838 (Clockwise)

Preceding gene: 94312706

Following gene: 94312708

Centisome position: 12.14

GC content: 60.97

Gene sequence:

>1704_bases
ATGCTGCAACCCACCCACGTACGCCCATCCGTGTTGCTCGATGGCGTGCGCTATCCCGATGAAGACCGCCTTCGCCGTTA
TGTCGGCGACGGTTACCTGACCGGCGAAACGCTGGTTGGCGCCTTCCGTCAATCGTTCCGCGATCACCATGATCGGCTGG
CCCTGGTGGGGCCGGAGGGCGAGTTTACCTATCAGGAGTTGGACGAACGGACTGACCGTCTTGGGGCGGCGCTGCTGGCG
CTGGGTCTCAGGCCGCTCGACCGTGCGGTCTTTCAATGCGGCAACAGCAATGAACTGCTGTTCGCATTTCTCGGATGCCT
GAAGGCCGGCATCATCCCGCTGTGTTCGCTACAGGCATTTCGCAAGCTGGAAGTGAGTTACCTGGGCAACTTGTGCGAGG
CACGTCTGCATTTCGTTCAGGGAGATGACCCCAAGTTCGACGACGTGGCGTTTGCCGAGGAGATGCAGGCGGAAGTGCCG
AGTCTCCAGTTCATCCTTCAGGCGCGCGGTGAGCGCCGCGGACGAGCCGTATTGCTGGCGGACTTGATCCGCGATATGCC
GCTGGCGCGCGCCCAGGCGTTGTTGGCTGAAGTCGACCACGAGCCGTTCCAGGCGGCTGTGTTCCAGTTGTCGGGTGGCA
CCACCGGTGTGCCGAAAATCATCCCGCGTTTTCAGAACGAGTATCTGTACAACATGCGCGCAGTGGCTGCATGCAACGGA
TATACCGCTGAAGACGTGCTGTTCTTCCCGACGCCGTACATGCACAACCTCAACATGGGCTGCTTCTTCGGCCCGTTTCT
GTTGTGCGGCGCGAAGGTGACGGTAGCCCCGGATATCGGCGAGGAAACGCTGCAGTCTCTCGTTCGCAACTACGAACCGA
CCTGGTTCGGCGTTGCCGGGCCGATCCTTAGTCGCATCGCGCCGGAACTGCTGCGCGGCACCGCGTCGGACAAGGCGCGA
CGCAACTTCATTGCCCCGAAGAACGCTGCCAACCTGACGCGCCTGACCGGATCGCCGACACATCATATTTTCGGCATGAC
CGAAGGCGTGATCATGTTCACGCGCCCGGAAGACCCTTCGGAGATTCGCGAGCAATCGATCGGTTATCCGGTATCCGATG
CCGACGAAGTGCGCATCGTCCATCCCGGCACCGAAGACACCGTGGGCGATGGCGAGGTCGGCGAAGCACTGTTTCGGGGG
CCGTACACCATCCGCGGCTATTACAAGTCCGAGAAGGAAGACGTTACGCGCTTCACTGCCGACGGCTTCTACCGCTCCGG
TGACCTGATGTCCGCTCAAGTGGTCGACGGCAAGCGCTATTACTTCTTCCTCGGGCGGATCAAGGATGTCGTCGATCGCG
GTGGCGAAAAGATCAACGCTGAAGAGCTCGAAAACGTCATCAACCAGCACCCGTCGATCCTGGCGTCAGCGGTCGTTGGC
ATGCCAGACAAGGTCTACGGCGAGCGTGTCTGCGCCTTTGTGCTACTCAAGCCGGCAGCGGCGTCGGGCCTGACACTTCC
CGAACTTGCCCAATACCTGCAGGAGGTCGGCCTCGCCAAGTTCAAGTGGCCGGAGAGGCTCGAAGTCATCCCGGAATTTC
CGCTGACGGCATCGGGCAAGCTCAGCAAGGTCCTGCTGCGTGACCGCATCGTCCAGATGCTGCGCACCGAAGCGGAAAAC
GACACTGTCCACCAAGCAAAGTGA

Upstream 100 bases:

>100_bases
GGGCAGCGCATTGCAGGACGTCATATCGTCGAATGGCTGCGGGAATCGATGGCCTGAGCGGCACCGCGGCCGAACTATAT
AACTACAGAGGTAGACAACG

Downstream 100 bases:

>100_bases
AGAAGGAAGAGACATGAACCAATCCACGCAAGGCGCCCACGTCAACGTTCCCGTCGTTGCACTGCACGGCGTACATCACA
CTGCGCGGCCCACCTGGAAA

Product: 2,3-dihydroxybenzoate-AMP ligase

Products: (2,3-Dihydroxybenzoyl)adenylate; Diphosphate [C]

Alternate protein names: TrsI; AMP-binding ligase; Quinoxaline-2-carboxylic acid-activating enzyme [H]

Number of amino acids: Translated: 567; Mature: 567

Protein sequence:

>567_residues
MLQPTHVRPSVLLDGVRYPDEDRLRRYVGDGYLTGETLVGAFRQSFRDHHDRLALVGPEGEFTYQELDERTDRLGAALLA
LGLRPLDRAVFQCGNSNELLFAFLGCLKAGIIPLCSLQAFRKLEVSYLGNLCEARLHFVQGDDPKFDDVAFAEEMQAEVP
SLQFILQARGERRGRAVLLADLIRDMPLARAQALLAEVDHEPFQAAVFQLSGGTTGVPKIIPRFQNEYLYNMRAVAACNG
YTAEDVLFFPTPYMHNLNMGCFFGPFLLCGAKVTVAPDIGEETLQSLVRNYEPTWFGVAGPILSRIAPELLRGTASDKAR
RNFIAPKNAANLTRLTGSPTHHIFGMTEGVIMFTRPEDPSEIREQSIGYPVSDADEVRIVHPGTEDTVGDGEVGEALFRG
PYTIRGYYKSEKEDVTRFTADGFYRSGDLMSAQVVDGKRYYFFLGRIKDVVDRGGEKINAEELENVINQHPSILASAVVG
MPDKVYGERVCAFVLLKPAAASGLTLPELAQYLQEVGLAKFKWPERLEVIPEFPLTASGKLSKVLLRDRIVQMLRTEAEN
DTVHQAK

Sequences:

>Translated_567_residues
MLQPTHVRPSVLLDGVRYPDEDRLRRYVGDGYLTGETLVGAFRQSFRDHHDRLALVGPEGEFTYQELDERTDRLGAALLA
LGLRPLDRAVFQCGNSNELLFAFLGCLKAGIIPLCSLQAFRKLEVSYLGNLCEARLHFVQGDDPKFDDVAFAEEMQAEVP
SLQFILQARGERRGRAVLLADLIRDMPLARAQALLAEVDHEPFQAAVFQLSGGTTGVPKIIPRFQNEYLYNMRAVAACNG
YTAEDVLFFPTPYMHNLNMGCFFGPFLLCGAKVTVAPDIGEETLQSLVRNYEPTWFGVAGPILSRIAPELLRGTASDKAR
RNFIAPKNAANLTRLTGSPTHHIFGMTEGVIMFTRPEDPSEIREQSIGYPVSDADEVRIVHPGTEDTVGDGEVGEALFRG
PYTIRGYYKSEKEDVTRFTADGFYRSGDLMSAQVVDGKRYYFFLGRIKDVVDRGGEKINAEELENVINQHPSILASAVVG
MPDKVYGERVCAFVLLKPAAASGLTLPELAQYLQEVGLAKFKWPERLEVIPEFPLTASGKLSKVLLRDRIVQMLRTEAEN
DTVHQAK
>Mature_567_residues
MLQPTHVRPSVLLDGVRYPDEDRLRRYVGDGYLTGETLVGAFRQSFRDHHDRLALVGPEGEFTYQELDERTDRLGAALLA
LGLRPLDRAVFQCGNSNELLFAFLGCLKAGIIPLCSLQAFRKLEVSYLGNLCEARLHFVQGDDPKFDDVAFAEEMQAEVP
SLQFILQARGERRGRAVLLADLIRDMPLARAQALLAEVDHEPFQAAVFQLSGGTTGVPKIIPRFQNEYLYNMRAVAACNG
YTAEDVLFFPTPYMHNLNMGCFFGPFLLCGAKVTVAPDIGEETLQSLVRNYEPTWFGVAGPILSRIAPELLRGTASDKAR
RNFIAPKNAANLTRLTGSPTHHIFGMTEGVIMFTRPEDPSEIREQSIGYPVSDADEVRIVHPGTEDTVGDGEVGEALFRG
PYTIRGYYKSEKEDVTRFTADGFYRSGDLMSAQVVDGKRYYFFLGRIKDVVDRGGEKINAEELENVINQHPSILASAVVG
MPDKVYGERVCAFVLLKPAAASGLTLPELAQYLQEVGLAKFKWPERLEVIPEFPLTASGKLSKVLLRDRIVQMLRTEAEN
DTVHQAK

Specific function: Involved in triostin biosynthesis. Activates quinoxaline-2-carboxylic acid (QA) via catalysis of the ATP- pyrophosphate exchange reaction dependent on QA, and the formation of the corresponding adenylate. Also activates structural analogs of QA such as qu

COG id: COG1021

COG function: function code Q; Peptide arylation enzymes

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI157311624, Length=517, Percent_Identity=24.1779497098646, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI157311622, Length=517, Percent_Identity=24.1779497098646, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI58082049, Length=517, Percent_Identity=24.1779497098646, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI38505220, Length=515, Percent_Identity=24.0776699029126, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI187761345, Length=560, Percent_Identity=25.1785714285714, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI187761343, Length=560, Percent_Identity=25.1785714285714, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI156151445, Length=554, Percent_Identity=23.826714801444, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI42544132, Length=524, Percent_Identity=23.6641221374046, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI115511026, Length=524, Percent_Identity=23.0916030534351, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI122937307, Length=225, Percent_Identity=32, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI28416953, Length=142, Percent_Identity=30.9859154929577, Blast_Score=77, Evalue=5e-14,
Organism=Escherichia coli, GI1786810, Length=547, Percent_Identity=32.1755027422303, Blast_Score=251, Evalue=1e-67,
Organism=Escherichia coli, GI145693145, Length=544, Percent_Identity=28.8602941176471, Blast_Score=180, Evalue=3e-46,
Organism=Escherichia coli, GI1788107, Length=561, Percent_Identity=23.5294117647059, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI221142682, Length=211, Percent_Identity=31.7535545023697, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1786801, Length=447, Percent_Identity=24.8322147651007, Blast_Score=69, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI71994703, Length=520, Percent_Identity=25.1923076923077, Blast_Score=110, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71994694, Length=520, Percent_Identity=25.1923076923077, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71994690, Length=520, Percent_Identity=25.1923076923077, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17538037, Length=527, Percent_Identity=23.1499051233397, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17557194, Length=508, Percent_Identity=24.0157480314961, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17560308, Length=555, Percent_Identity=25.9459459459459, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17558820, Length=495, Percent_Identity=22.6262626262626, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI32563687, Length=519, Percent_Identity=23.5067437379576, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17559526, Length=544, Percent_Identity=22.7941176470588, Blast_Score=80, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17531443, Length=548, Percent_Identity=24.0875912408759, Blast_Score=75, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI71985884, Length=605, Percent_Identity=23.4710743801653, Blast_Score=72, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=359, Percent_Identity=25.3481894150418, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6319264, Length=135, Percent_Identity=32.5925925925926, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24581924, Length=554, Percent_Identity=24.187725631769, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI21355181, Length=524, Percent_Identity=22.1374045801527, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI18859661, Length=356, Percent_Identity=24.1573033707865, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24648260, Length=499, Percent_Identity=23.6472945891784, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI21358303, Length=194, Percent_Identity=27.8350515463918, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 2.7.7.58; 2.3.1.- [C]

Molecular weight: Translated: 63057; Mature: 63057

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQPTHVRPSVLLDGVRYPDEDRLRRYVGDGYLTGETLVGAFRQSFRDHHDRLALVGPEG
CCCCCCCCHHHHCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCC
EFTYQELDERTDRLGAALLALGLRPLDRAVFQCGNSNELLFAFLGCLKAGIIPLCSLQAF
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RKLEVSYLGNLCEARLHFVQGDDPKFDDVAFAEEMQAEVPSLQFILQARGERRGRAVLLA
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHH
DLIRDMPLARAQALLAEVDHEPFQAAVFQLSGGTTGVPKIIPRFQNEYLYNMRAVAACNG
HHHHCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
YTAEDVLFFPTPYMHNLNMGCFFGPFLLCGAKVTVAPDIGEETLQSLVRNYEPTWFGVAG
CCCCCEEECCCCCCCCCCCHHHHHHHHHHCCCEEECCCCCHHHHHHHHHCCCCCEEEHHH
PILSRIAPELLRGTASDKARRNFIAPKNAANLTRLTGSPTHHIFGMTEGVIMFTRPEDPS
HHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCEECCCCCCCEEEECCCCEEEEECCCCHH
EIREQSIGYPVSDADEVRIVHPGTEDTVGDGEVGEALFRGPYTIRGYYKSEKEDVTRFTA
HHHHHHCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHCCCEEEEEEECCCHHHHHHHHH
DGFYRSGDLMSAQVVDGKRYYFFLGRIKDVVDRGGEKINAEELENVINQHPSILASAVVG
CCCEECCCCEEEEEECCCEEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHCHHHHHHHHHC
MPDKVYGERVCAFVLLKPAAASGLTLPELAQYLQEVGLAKFKWPERLEVIPEFPLTASGK
CCHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCH
LSKVLLRDRIVQMLRTEAENDTVHQAK
HHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLQPTHVRPSVLLDGVRYPDEDRLRRYVGDGYLTGETLVGAFRQSFRDHHDRLALVGPEG
CCCCCCCCHHHHCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCC
EFTYQELDERTDRLGAALLALGLRPLDRAVFQCGNSNELLFAFLGCLKAGIIPLCSLQAF
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RKLEVSYLGNLCEARLHFVQGDDPKFDDVAFAEEMQAEVPSLQFILQARGERRGRAVLLA
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHH
DLIRDMPLARAQALLAEVDHEPFQAAVFQLSGGTTGVPKIIPRFQNEYLYNMRAVAACNG
HHHHCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
YTAEDVLFFPTPYMHNLNMGCFFGPFLLCGAKVTVAPDIGEETLQSLVRNYEPTWFGVAG
CCCCCEEECCCCCCCCCCCHHHHHHHHHHCCCEEECCCCCHHHHHHHHHCCCCCEEEHHH
PILSRIAPELLRGTASDKARRNFIAPKNAANLTRLTGSPTHHIFGMTEGVIMFTRPEDPS
HHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCEECCCCCCCEEEECCCCEEEEECCCCHH
EIREQSIGYPVSDADEVRIVHPGTEDTVGDGEVGEALFRGPYTIRGYYKSEKEDVTRFTA
HHHHHHCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHCCCEEEEEEECCCHHHHHHHHH
DGFYRSGDLMSAQVVDGKRYYFFLGRIKDVVDRGGEKINAEELENVINQHPSILASAVVG
CCCEECCCCEEEEEECCCEEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHCHHHHHHHHHC
MPDKVYGERVCAFVLLKPAAASGLTLPELAQYLQEVGLAKFKWPERLEVIPEFPLTASGK
CCHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCH
LSKVLLRDRIVQMLRTEAENDTVHQAK
HHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2,3-Dihydroxybenzoate; ATP [C]

Specific reaction: 2,3-Dihydroxybenzoate + ATP <==> (2,3-Dihydroxybenzoyl)adenylate + Diphosphate [C]

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA