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The map label for this gene is ligB [H]

Identifier: 94312641

GI number: 94312641

Start: 251647

End: 252939

Strand: Direct

Name: ligB [H]

Synonym: Rmet_3709

Alternate gene names: 94312641

Gene position: 251647-252939 (Clockwise)

Preceding gene: 94312640

Following gene: 94312642

Centisome position: 9.75

GC content: 63.81

Gene sequence:

>1293_bases
ATGGCCACCATCATCGGCGCAATCGCCTCATCCCATACGCCAACCATCGGATTCGCGCTCGACGCGGGCAAGCAACAGGA
CCCCGCCTGGGCGCCGATCTTCGCGGCCTATGCGCCGGTGAGCGAGTGGCTCGCTAGGAAAAAGCCCGACGTGCTGCTCG
TCGTCTACAACGATCACGTCACGTCGTTCTTCTTCGATCACTATTCGGCCTTTGCGCTGGGTATCGGCGATACCTACCAT
GCTGCCGATGAGGGCGGTGGCCCGCGTAACCTGCCGCCGGCGCGCGGCCACGTCGAACTGGCGCGGCACGTCGGCGCATC
GCTGGTGGCCGACGAGTTCGACATGTCGTTCTTCCAGGACAAGCCACTCGATCATGGTTGCTTCTCGCCGATGTCGGTCA
TGCTGCCGCACGAGGGCGAGTGGCCCGTGAAGATTCTGCCGCTGCAGGTGGGCGTGCTGCAGTTCCCGGCACCGTCGGCG
CGTCGTTGCTACAAGCTCGGACAGGCGATCCGCCGCGCGGTGGAGAGCTACCCGGAAGACCTCAAGGTGGCGATCGTCGC
CACCGGTGGTCTGTCGCACCAGGTGCATGGCGAGCGTGCTGGCTTCAACAATACCGCGTGGGATCACCAGTTCCTCGACC
TGTTCGAATCCGATCCCGAGTCGCTGCTAGACATCACCCATGCCGAGTACGCGAAGCGAGGCGGCTTCGAGAGCGCGGAA
GTGGTCATGTGGATGGTGATGCGTGGCGCGATGTCGAGCCGTGTCCGCTGCCTGCATCGTGATTACTATCTGCCGTCGAT
GACCGGCATCGCAGTGGCGGTCTACGAGAACGAATCCGCCGACGCCCCCGCGCTGCGAGAGGCGCGTGCCGAACGCCAGC
GCGAGCACATTGGTCATCAGTTGCACGGGATCGAGCAACTGGACGGGACGTACCCGTTCACGCTGGAAACCAGCGTGCGC
GCATACCGGATCAACCGCTATCTGCATCAACTTATCGATCCGGCATTCCGCGAGCGATTCCGCGCCGATCCGCTGGCAAC
CTACGACGAGGCGGCCCTGACGCCGGAAGAGCGCGACATGATCACGCGCCGCGACTGGCGCGGCATGATCCACTACGGCG
TGATTTTCTTCATGCTGGAGAAGCTCGGCGCGGTCTGCGGTGTCTCGAACCTGCATATCTACGCGGCGATGCGTGGCCAG
TCCCTTGAAGACTTCCAGAAGACGCGCAATGCGCCGGGTGCGCTGTACTCGGTGGCCGGTAGCGGCAGCGCTTCATGGAG
TGCGAAGTCCTGA

Upstream 100 bases:

>100_bases
CGCGGTGATTGCCATCCTGGCCGTTCCGGCGACCATGGCGGCAATCTCCATTGTTTCCACGAAGGCGCAGCCTCGCGCCG
TAACCGCTTGACGTATTGCC

Downstream 100 bases:

>100_bases
CCGGACGATGTCGGGGGCTTGAATTCCGGCGCAATGCCGCCATGTGCGAGGCTGTTTTTGACTCGCACCAGCCCCCGCTC
GGTTTCCGTATGTACAAGAA

Product: protocatechuate 4,5-dioxygenase

Products: NA

Alternate protein names: 4,5-PCD [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MATIIGAIASSHTPTIGFALDAGKQQDPAWAPIFAAYAPVSEWLARKKPDVLLVVYNDHVTSFFFDHYSAFALGIGDTYH
AADEGGGPRNLPPARGHVELARHVGASLVADEFDMSFFQDKPLDHGCFSPMSVMLPHEGEWPVKILPLQVGVLQFPAPSA
RRCYKLGQAIRRAVESYPEDLKVAIVATGGLSHQVHGERAGFNNTAWDHQFLDLFESDPESLLDITHAEYAKRGGFESAE
VVMWMVMRGAMSSRVRCLHRDYYLPSMTGIAVAVYENESADAPALREARAERQREHIGHQLHGIEQLDGTYPFTLETSVR
AYRINRYLHQLIDPAFRERFRADPLATYDEAALTPEERDMITRRDWRGMIHYGVIFFMLEKLGAVCGVSNLHIYAAMRGQ
SLEDFQKTRNAPGALYSVAGSGSASWSAKS

Sequences:

>Translated_430_residues
MATIIGAIASSHTPTIGFALDAGKQQDPAWAPIFAAYAPVSEWLARKKPDVLLVVYNDHVTSFFFDHYSAFALGIGDTYH
AADEGGGPRNLPPARGHVELARHVGASLVADEFDMSFFQDKPLDHGCFSPMSVMLPHEGEWPVKILPLQVGVLQFPAPSA
RRCYKLGQAIRRAVESYPEDLKVAIVATGGLSHQVHGERAGFNNTAWDHQFLDLFESDPESLLDITHAEYAKRGGFESAE
VVMWMVMRGAMSSRVRCLHRDYYLPSMTGIAVAVYENESADAPALREARAERQREHIGHQLHGIEQLDGTYPFTLETSVR
AYRINRYLHQLIDPAFRERFRADPLATYDEAALTPEERDMITRRDWRGMIHYGVIFFMLEKLGAVCGVSNLHIYAAMRGQ
SLEDFQKTRNAPGALYSVAGSGSASWSAKS
>Mature_429_residues
ATIIGAIASSHTPTIGFALDAGKQQDPAWAPIFAAYAPVSEWLARKKPDVLLVVYNDHVTSFFFDHYSAFALGIGDTYHA
ADEGGGPRNLPPARGHVELARHVGASLVADEFDMSFFQDKPLDHGCFSPMSVMLPHEGEWPVKILPLQVGVLQFPAPSAR
RCYKLGQAIRRAVESYPEDLKVAIVATGGLSHQVHGERAGFNNTAWDHQFLDLFESDPESLLDITHAEYAKRGGFESAEV
VMWMVMRGAMSSRVRCLHRDYYLPSMTGIAVAVYENESADAPALREARAERQREHIGHQLHGIEQLDGTYPFTLETSVRA
YRINRYLHQLIDPAFRERFRADPLATYDEAALTPEERDMITRRDWRGMIHYGVIFFMLEKLGAVCGVSNLHIYAAMRGQS
LEDFQKTRNAPGALYSVAGSGSASWSAKS

Specific function: Responsible for the aromatic ring fission of protocatechuate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786544, Length=288, Percent_Identity=24.3055555555556, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004183 [H]

Pfam domain/function: PF02900 LigB [H]

EC number: =1.13.11.8 [H]

Molecular weight: Translated: 47829; Mature: 47697

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIIGAIASSHTPTIGFALDAGKQQDPAWAPIFAAYAPVSEWLARKKPDVLLVVYNDHV
CCHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCH
TSFFFDHYSAFALGIGDTYHAADEGGGPRNLPPARGHVELARHVGASLVADEFDMSFFQD
HHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHC
KPLDHGCFSPMSVMLPHEGEWPVKILPLQVGVLQFPAPSARRCYKLGQAIRRAVESYPED
CCCCCCCCCCCEEECCCCCCCCEEEEEEEECEEECCCCHHHHHHHHHHHHHHHHHCCCCC
LKVAIVATGGLSHQVHGERAGFNNTAWDHQFLDLFESDPESLLDITHAEYAKRGGFESAE
EEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHH
VVMWMVMRGAMSSRVRCLHRDYYLPSMTGIAVAVYENESADAPALREARAERQREHIGHQ
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
LHGIEQLDGTYPFTLETSVRAYRINRYLHQLIDPAFRERFRADPLATYDEAALTPEERDM
HCCHHHHCCCCCEEEECCHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCCHHHHH
ITRRDWRGMIHYGVIFFMLEKLGAVCGVSNLHIYAAMRGQSLEDFQKTRNAPGALYSVAG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHCCCCCCEEEECC
SGSASWSAKS
CCCCCCCCCC
>Mature Secondary Structure 
ATIIGAIASSHTPTIGFALDAGKQQDPAWAPIFAAYAPVSEWLARKKPDVLLVVYNDHV
CHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCH
TSFFFDHYSAFALGIGDTYHAADEGGGPRNLPPARGHVELARHVGASLVADEFDMSFFQD
HHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHC
KPLDHGCFSPMSVMLPHEGEWPVKILPLQVGVLQFPAPSARRCYKLGQAIRRAVESYPED
CCCCCCCCCCCEEECCCCCCCCEEEEEEEECEEECCCCHHHHHHHHHHHHHHHHHCCCCC
LKVAIVATGGLSHQVHGERAGFNNTAWDHQFLDLFESDPESLLDITHAEYAKRGGFESAE
EEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHH
VVMWMVMRGAMSSRVRCLHRDYYLPSMTGIAVAVYENESADAPALREARAERQREHIGHQ
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
LHGIEQLDGTYPFTLETSVRAYRINRYLHQLIDPAFRERFRADPLATYDEAALTPEERDM
HCCHHHHCCCCCEEEECCHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCCHHHHH
ITRRDWRGMIHYGVIFFMLEKLGAVCGVSNLHIYAAMRGQSLEDFQKTRNAPGALYSVAG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHCCCCCCEEEECC
SGSASWSAKS
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2185230 [H]