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The map label for this gene is pcaK2 [H]

Identifier: 94312640

GI number: 94312640

Start: 250312

End: 251637

Strand: Direct

Name: pcaK2 [H]

Synonym: Rmet_3708

Alternate gene names: 94312640

Gene position: 250312-251637 (Clockwise)

Preceding gene: 94312639

Following gene: 94312641

Centisome position: 9.7

GC content: 65.69

Gene sequence:

>1326_bases
ATGACAACGCAACGGCTCGACATTCGGGCCCATATCAACGAACGCAAGATGTCCGGCTACCAGTGGCTGCTGCTGGTGCT
GTGTTTCCTGATCGTCGCCACGGACGGGATGGATGTGGCCATCATGGGCTTCCTCGCGCCGGACATCACACGCGAATGGG
GCATCTCGAAGGCCGCCTTTGGCGTGGTGATGAGCGCCGCGCCGATCGGCCTGGCTATCGGCGCGCTGGTGGTCGGACCG
CTGTCCGACCGCTTCGGCCGCAAGAAGCTGCTGGTCGGTTCCATCGCGCTGTTCGGCGTATTCAATCTGCTGAGCGCATT
CGCCGATGGCACGGCAACCTTGTCGCTGCTGCGCTTCCTGACCGGCCTTGGTCTGGGCGCGGCCATGCCGAGCACGACCA
CACTGCTCTCCGAGTATGTGCCGGAACGTTCGCGCAGCATGTTGCTGGCGACGATGTTCACGGGCTTCAACCTCGGCTCC
GCGCTGGTGGGCTTCGGTGCCGCCGCGCTTCTGCCGCATCATGGCTGGCGCGCGGTGCTGGTCGCCGGTGGCGCGATTCC
GCTGATCTGCCTGCCGTTCTACATCATGCTGATTCCGGAATCGGCGCGCTTCATGGTGGTCCGCAATGTCGCGCCGGCGC
GAATCGCCCGCACGCTGCGCCGCGTGATCGGCAGCGTGGTGGCCGATGACACCGTGTTCACGATCAGCGAGCCGCCGGTT
TCCGGCAAGCAGCCCGTCCGCACGCTGTTGTCGTCGGGCTATCGCAGCATGACGCTGGCGCTGTGGGTCACGTACTTCAT
GGGGCTGCTCGTGATCTACCTGCTGAGCGGCTGGCTGCCCACGCTGATCAAGGACGCCGGCCTGCCGATCGATCGCGCCG
CCAACCTGACGGCACTGTTCCAGCTCGGCGGTACCGTCGGCGCGCTGGTGGTGGGCTACTGCATGGACCGCTTCGCGCCC
CATCGCGTGATCGGCGTGTCATATGTGCTGGGCGCGATGTTCATCCTGATGCTGGCTTCGGGCAGCGTCACGTCGTCGAT
GTTCGCACTCTACGTGCTGCTGGCCGGCTTCTGCATGAGCGGCGCGCAGACCGGGCTCAACGCTTTCGCGCCGCAGTGCT
ATCCCACGCCGGTGCGTGCCACTGGCGTAAGCTGGATGCTCGGCATCGGCCGCTTCGGCAGCATCACCGGCTCCATGGCG
GGCGGTGTGCTGCTGTCGATGGGCTGGAGTTTCAGCGCGGTGATTGCCATCCTGGCCGTTCCGGCGACCATGGCGGCAAT
CTCCATTGTTTCCACGAAGGCGCAGCCTCGCGCCGTAACCGCTTGA

Upstream 100 bases:

>100_bases
CATGGGGGATGCGCGGCCGGCCGCTTCCGCGGCGATTAGCCATGTCGATAACTAGAATCCGCTGACTCCCGCACGGCGGA
TCACGCCAGGAGACGGCAAC

Downstream 100 bases:

>100_bases
CGTATTGCCATGGCCACCATCATCGGCGCAATCGCCTCATCCCATACGCCAACCATCGGATTCGCGCTCGACGCGGGCAA
GCAACAGGACCCCGCCTGGG

Product: major facilitator superfamily transporter

Products: Proton [Cytoplasm]; 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MTTQRLDIRAHINERKMSGYQWLLLVLCFLIVATDGMDVAIMGFLAPDITREWGISKAAFGVVMSAAPIGLAIGALVVGP
LSDRFGRKKLLVGSIALFGVFNLLSAFADGTATLSLLRFLTGLGLGAAMPSTTTLLSEYVPERSRSMLLATMFTGFNLGS
ALVGFGAAALLPHHGWRAVLVAGGAIPLICLPFYIMLIPESARFMVVRNVAPARIARTLRRVIGSVVADDTVFTISEPPV
SGKQPVRTLLSSGYRSMTLALWVTYFMGLLVIYLLSGWLPTLIKDAGLPIDRAANLTALFQLGGTVGALVVGYCMDRFAP
HRVIGVSYVLGAMFILMLASGSVTSSMFALYVLLAGFCMSGAQTGLNAFAPQCYPTPVRATGVSWMLGIGRFGSITGSMA
GGVLLSMGWSFSAVIAILAVPATMAAISIVSTKAQPRAVTA

Sequences:

>Translated_441_residues
MTTQRLDIRAHINERKMSGYQWLLLVLCFLIVATDGMDVAIMGFLAPDITREWGISKAAFGVVMSAAPIGLAIGALVVGP
LSDRFGRKKLLVGSIALFGVFNLLSAFADGTATLSLLRFLTGLGLGAAMPSTTTLLSEYVPERSRSMLLATMFTGFNLGS
ALVGFGAAALLPHHGWRAVLVAGGAIPLICLPFYIMLIPESARFMVVRNVAPARIARTLRRVIGSVVADDTVFTISEPPV
SGKQPVRTLLSSGYRSMTLALWVTYFMGLLVIYLLSGWLPTLIKDAGLPIDRAANLTALFQLGGTVGALVVGYCMDRFAP
HRVIGVSYVLGAMFILMLASGSVTSSMFALYVLLAGFCMSGAQTGLNAFAPQCYPTPVRATGVSWMLGIGRFGSITGSMA
GGVLLSMGWSFSAVIAILAVPATMAAISIVSTKAQPRAVTA
>Mature_440_residues
TTQRLDIRAHINERKMSGYQWLLLVLCFLIVATDGMDVAIMGFLAPDITREWGISKAAFGVVMSAAPIGLAIGALVVGPL
SDRFGRKKLLVGSIALFGVFNLLSAFADGTATLSLLRFLTGLGLGAAMPSTTTLLSEYVPERSRSMLLATMFTGFNLGSA
LVGFGAAALLPHHGWRAVLVAGGAIPLICLPFYIMLIPESARFMVVRNVAPARIARTLRRVIGSVVADDTVFTISEPPVS
GKQPVRTLLSSGYRSMTLALWVTYFMGLLVIYLLSGWLPTLIKDAGLPIDRAANLTALFQLGGTVGALVVGYCMDRFAPH
RVIGVSYVLGAMFILMLASGSVTSSMFALYVLLAGFCMSGAQTGLNAFAPQCYPTPVRATGVSWMLGIGRFGSITGSMAG
GVLLSMGWSFSAVIAILAVPATMAAISIVSTKAQPRAVTA

Specific function: Transporter for 4-hydroxybenzoate. Also required for chemotaxis to aromatic acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Homo sapiens, GI24308167, Length=424, Percent_Identity=26.6509433962264, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI213021148, Length=439, Percent_Identity=27.3348519362187, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI7662270, Length=313, Percent_Identity=23.961661341853, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI216548223, Length=401, Percent_Identity=24.6882793017456, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI73695465, Length=207, Percent_Identity=27.0531400966184, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI8923870, Length=344, Percent_Identity=25, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI262399383, Length=264, Percent_Identity=24.2424242424242, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI87081723, Length=386, Percent_Identity=34.7150259067358, Blast_Score=185, Evalue=4e-48,
Organism=Escherichia coli, GI87082231, Length=203, Percent_Identity=27.5862068965517, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI87082159, Length=367, Percent_Identity=26.4305177111717, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI87082404, Length=385, Percent_Identity=22.5974025974026, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788068, Length=398, Percent_Identity=23.8693467336683, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788074, Length=377, Percent_Identity=25.1989389920424, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789152, Length=319, Percent_Identity=21.9435736677116, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1786229, Length=389, Percent_Identity=23.1362467866324, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1789515, Length=325, Percent_Identity=22.7692307692308, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI48994889, Length=174, Percent_Identity=28.735632183908, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI32564663, Length=398, Percent_Identity=23.3668341708543, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI19922874, Length=407, Percent_Identity=26.2899262899263, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24640198, Length=462, Percent_Identity=23.1601731601732, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24640196, Length=462, Percent_Identity=23.1601731601732, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24640200, Length=462, Percent_Identity=23.1601731601732, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004746
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 46532; Mature: 46401

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQRLDIRAHINERKMSGYQWLLLVLCFLIVATDGMDVAIMGFLAPDITREWGISKAAF
CCCCEEEHEECCCHHHHCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHH
GVVMSAAPIGLAIGALVVGPLSDRFGRKKLLVGSIALFGVFNLLSAFADGTATLSLLRFL
HHHHHHCHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
TGLGLGAAMPSTTTLLSEYVPERSRSMLLATMFTGFNLGSALVGFGAAALLPHHGWRAVL
HHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHCCHHHHCCCCCCEEEE
VAGGAIPLICLPFYIMLIPESARFMVVRNVAPARIARTLRRVIGSVVADDTVFTISEPPV
EECCCHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCCCC
SGKQPVRTLLSSGYRSMTLALWVTYFMGLLVIYLLSGWLPTLIKDAGLPIDRAANLTALF
CCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCHHHHH
QLGGTVGALVVGYCMDRFAPHRVIGVSYVLGAMFILMLASGSVTSSMFALYVLLAGFCMS
HHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
GAQTGLNAFAPQCYPTPVRATGVSWMLGIGRFGSITGSMAGGVLLSMGWSFSAVIAILAV
HHHHCHHHCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHCCHHHHCCCCHHHHHHHHHH
PATMAAISIVSTKAQPRAVTA
HHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TTQRLDIRAHINERKMSGYQWLLLVLCFLIVATDGMDVAIMGFLAPDITREWGISKAAF
CCCEEEHEECCCHHHHCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHH
GVVMSAAPIGLAIGALVVGPLSDRFGRKKLLVGSIALFGVFNLLSAFADGTATLSLLRFL
HHHHHHCHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
TGLGLGAAMPSTTTLLSEYVPERSRSMLLATMFTGFNLGSALVGFGAAALLPHHGWRAVL
HHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHCCHHHHCCCCCCEEEE
VAGGAIPLICLPFYIMLIPESARFMVVRNVAPARIARTLRRVIGSVVADDTVFTISEPPV
EECCCHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCCCC
SGKQPVRTLLSSGYRSMTLALWVTYFMGLLVIYLLSGWLPTLIKDAGLPIDRAANLTALF
CCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCHHHHH
QLGGTVGALVVGYCMDRFAPHRVIGVSYVLGAMFILMLASGSVTSSMFALYVLLAGFCMS
HHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
GAQTGLNAFAPQCYPTPVRATGVSWMLGIGRFGSITGSMAGGVLLSMGWSFSAVIAILAV
HHHHCHHHCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHCCHHHHCCCCHHHHHHHHHH
PATMAAISIVSTKAQPRAVTA
HHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 3-(3-hydroxyphenyl)propionate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 3-(3-hydroxyphenyl)propionate [Periplasm] = Proton [Cytoplasm] + 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961399 [H]