Click here to switch to the map view.

The map label for this gene is rsmB [H]

Identifier: 93007290

GI number: 93007290

Start: 3047079

End: 3048653

Strand: Reverse

Name: rsmB [H]

Synonym: Pcryo_2466

Alternate gene names: 93007290

Gene position: 3048653-3047079 (Counterclockwise)

Preceding gene: 93007291

Following gene: 93007289

Centisome position: 99.63

GC content: 43.87

Gene sequence:

>1575_bases
ATGAGACAAACCAGCACTGCACCAAGAAATAACAAGCTCAAACCGTCAAGCAACCTTATCATGAATGGTAGCGTTCGCGT
TCGCGTGATTCGCACTTTGCTGGCCATTCAAAATGGTCAGTCGCTAGCAAGCGTCCTTGATCCGTTACTAAATAGTCTTC
ATGATGGGGATAAAGGTTTTGCTCATGCGTTATTGCTAACGACGTTGCGCCAGTGGTATGCCCTTGAGCGTCTACTCGAT
AGTCTGGCTGATAACTCGATTGATGAAAAAGAAGTACGTACGACGATTCAAGTAGGTTTGACGCAGCTGCTCTATTTGCA
AGTGGCTGACCATGCGGCAATTCATGAAACGGTTGAAGCAGCAAAAGAAATTGAGTTTGCGCATGCGACGGGTTTAATCA
ATGCCATCTTGCGTAAAGTTGCTAAGAACCCAAGTAAGTTCCGTAAGAAGTGTGATAAAAAACACAGCTTACCGAATTGG
CTTGCCTATCAGCTTAAGCAAGATTGGAGTGAGCAGTATGACGATTTGATGCAGGGTCTACGTCAACCTGCGCCAATGTT
TTTGCGAGTAAATACAGCGGTTACTTCGATAGAGGACTATCAGCAAGCATTAGTGGAATTTGAAGTTGAAGCGGACTTAG
TTGAGCTAACAAGTGGCTTACAACTCGAAAACTCGTCGGATACAGCCACTTCTAATAGTATAGTAACCAAAGGTCTTAGA
CTACATCAAAGCACTGCAGTCAATGCCTTACCACAATTTGCCCACGGGGCGGCTAGTGTGCAAGATATGCATGCTCAGCT
TGCAGCACCGCTTATCCATAGCGCTCTACTCAATAAGCTGAGTGTTAAAAACGATGATGCAGACGGCGATATGGATACGA
GCAACAATGAGATTCGTATTTTAGATGCCTGTGCAGCGCCGGGTGGTAAGCTTGCTCATTGGTTAGAATTAATTAGTTCA
GACGAGCAATCACCTTTGTTTCACGTGAAACAAAACGAAGAATTTGTTTCTCTAGTTTCGAAAGTGAAGCTAACTGCGAT
AGATAATGAAGCGCCGCGTATCGAGCGTATTTATGATAATTTACAGCGCTTGAATTTAAGTCAGCATGATTTACAGCAAG
CTGAGAAAAATATTGAGCTCAATATTGTTTGTGCTGATGCGACCACATGGCAAGGGGCTGGTAAGAAAAAGAGCAAGCTC
GTATTTGACGCTATATTGCTAGACTCACCTTGTACGGCGACTGGCGTGATTCGTCGCCATCCTGATATCAGTATCTTGCG
TACTGAAGAAGACGTCGAACAAACCGCACTTTTGCAAGCCGATATCTTGCATAATCTATGGCCACAACTTAAGGTTGGCG
GTTATTTGTTATATGTTACTTGCTCTATCCTAAAGCAAGAGAACGTCGAGCAGATGCAGGATTTCATGACTCATTATAAT
AATGTAGCAGCTGTTGAATTTAGCGACGGTTGCAACTGGGGTATCGAACAAGCGATTGGTCGCCAGTGTTTGCCAATTGA
TAGCAAGAGCGGTGATGGTTTTTATTATGCCTTGCTGCAGAAAACTGCTGAATAA

Upstream 100 bases:

>100_bases
GGCGACCAGTTTAGTAAAGAAACGAAATAACTCCATTTAGATATACCTAATCCAAATCGCACAAGACGCTAACTATTAGA
CGTCAAGGAAATTCAATTTA

Downstream 100 bases:

>100_bases
ACCGTTTCAACTAATAATCTCATTGTAGACAGGACAATCTAATGAAATATATCAGTACCCGTGGTCAGACAGCGCCGATG
GATTTTAGTGATGTGTTATT

Product: Fmu (Sun)

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 524; Mature: 524

Protein sequence:

>524_residues
MRQTSTAPRNNKLKPSSNLIMNGSVRVRVIRTLLAIQNGQSLASVLDPLLNSLHDGDKGFAHALLLTTLRQWYALERLLD
SLADNSIDEKEVRTTIQVGLTQLLYLQVADHAAIHETVEAAKEIEFAHATGLINAILRKVAKNPSKFRKKCDKKHSLPNW
LAYQLKQDWSEQYDDLMQGLRQPAPMFLRVNTAVTSIEDYQQALVEFEVEADLVELTSGLQLENSSDTATSNSIVTKGLR
LHQSTAVNALPQFAHGAASVQDMHAQLAAPLIHSALLNKLSVKNDDADGDMDTSNNEIRILDACAAPGGKLAHWLELISS
DEQSPLFHVKQNEEFVSLVSKVKLTAIDNEAPRIERIYDNLQRLNLSQHDLQQAEKNIELNIVCADATTWQGAGKKKSKL
VFDAILLDSPCTATGVIRRHPDISILRTEEDVEQTALLQADILHNLWPQLKVGGYLLYVTCSILKQENVEQMQDFMTHYN
NVAAVEFSDGCNWGIEQAIGRQCLPIDSKSGDGFYYALLQKTAE

Sequences:

>Translated_524_residues
MRQTSTAPRNNKLKPSSNLIMNGSVRVRVIRTLLAIQNGQSLASVLDPLLNSLHDGDKGFAHALLLTTLRQWYALERLLD
SLADNSIDEKEVRTTIQVGLTQLLYLQVADHAAIHETVEAAKEIEFAHATGLINAILRKVAKNPSKFRKKCDKKHSLPNW
LAYQLKQDWSEQYDDLMQGLRQPAPMFLRVNTAVTSIEDYQQALVEFEVEADLVELTSGLQLENSSDTATSNSIVTKGLR
LHQSTAVNALPQFAHGAASVQDMHAQLAAPLIHSALLNKLSVKNDDADGDMDTSNNEIRILDACAAPGGKLAHWLELISS
DEQSPLFHVKQNEEFVSLVSKVKLTAIDNEAPRIERIYDNLQRLNLSQHDLQQAEKNIELNIVCADATTWQGAGKKKSKL
VFDAILLDSPCTATGVIRRHPDISILRTEEDVEQTALLQADILHNLWPQLKVGGYLLYVTCSILKQENVEQMQDFMTHYN
NVAAVEFSDGCNWGIEQAIGRQCLPIDSKSGDGFYYALLQKTAE
>Mature_524_residues
MRQTSTAPRNNKLKPSSNLIMNGSVRVRVIRTLLAIQNGQSLASVLDPLLNSLHDGDKGFAHALLLTTLRQWYALERLLD
SLADNSIDEKEVRTTIQVGLTQLLYLQVADHAAIHETVEAAKEIEFAHATGLINAILRKVAKNPSKFRKKCDKKHSLPNW
LAYQLKQDWSEQYDDLMQGLRQPAPMFLRVNTAVTSIEDYQQALVEFEVEADLVELTSGLQLENSSDTATSNSIVTKGLR
LHQSTAVNALPQFAHGAASVQDMHAQLAAPLIHSALLNKLSVKNDDADGDMDTSNNEIRILDACAAPGGKLAHWLELISS
DEQSPLFHVKQNEEFVSLVSKVKLTAIDNEAPRIERIYDNLQRLNLSQHDLQQAEKNIELNIVCADATTWQGAGKKKSKL
VFDAILLDSPCTATGVIRRHPDISILRTEEDVEQTALLQADILHNLWPQLKVGGYLLYVTCSILKQENVEQMQDFMTHYN
NVAAVEFSDGCNWGIEQAIGRQCLPIDSKSGDGFYYALLQKTAE

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=325, Percent_Identity=27.0769230769231, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI76150623, Length=325, Percent_Identity=27.0769230769231, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI2367212, Length=495, Percent_Identity=32.7272727272727, Blast_Score=233, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI17536757, Length=358, Percent_Identity=24.0223463687151, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI22024126, Length=240, Percent_Identity=28.3333333333333, Blast_Score=85, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 58290; Mature: 58290

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQTSTAPRNNKLKPSSNLIMNGSVRVRVIRTLLAIQNGQSLASVLDPLLNSLHDGDKGF
CCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHH
AHALLLTTLRQWYALERLLDSLADNSIDEKEVRTTIQVGLTQLLYLQVADHAAIHETVEA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKEIEFAHATGLINAILRKVAKNPSKFRKKCDKKHSLPNWLAYQLKQDWSEQYDDLMQGL
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
RQPAPMFLRVNTAVTSIEDYQQALVEFEVEADLVELTSGLQLENSSDTATSNSIVTKGLR
CCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCHHHHHHHH
LHQSTAVNALPQFAHGAASVQDMHAQLAAPLIHSALLNKLSVKNDDADGDMDTSNNEIRI
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEE
LDACAAPGGKLAHWLELISSDEQSPLFHVKQNEEFVSLVSKVKLTAIDNEAPRIERIYDN
EEECCCCCHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHEEEECCCCHHHHHHHHH
LQRLNLSQHDLQQAEKNIELNIVCADATTWQGAGKKKSKLVFDAILLDSPCTATGVIRRH
HHHCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHCCC
PDISILRTEEDVEQTALLQADILHNLWPQLKVGGYLLYVTCSILKQENVEQMQDFMTHYN
CCCEEEECHHHHHHHHHHHHHHHHHHCCCHHHCCEEEHHHHHHHHHCCHHHHHHHHHHCC
NVAAVEFSDGCNWGIEQAIGRQCLPIDSKSGDGFYYALLQKTAE
CEEEEEECCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MRQTSTAPRNNKLKPSSNLIMNGSVRVRVIRTLLAIQNGQSLASVLDPLLNSLHDGDKGF
CCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHH
AHALLLTTLRQWYALERLLDSLADNSIDEKEVRTTIQVGLTQLLYLQVADHAAIHETVEA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKEIEFAHATGLINAILRKVAKNPSKFRKKCDKKHSLPNWLAYQLKQDWSEQYDDLMQGL
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
RQPAPMFLRVNTAVTSIEDYQQALVEFEVEADLVELTSGLQLENSSDTATSNSIVTKGLR
CCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCHHHHHHHH
LHQSTAVNALPQFAHGAASVQDMHAQLAAPLIHSALLNKLSVKNDDADGDMDTSNNEIRI
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEE
LDACAAPGGKLAHWLELISSDEQSPLFHVKQNEEFVSLVSKVKLTAIDNEAPRIERIYDN
EEECCCCCHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHEEEECCCCHHHHHHHHH
LQRLNLSQHDLQQAEKNIELNIVCADATTWQGAGKKKSKLVFDAILLDSPCTATGVIRRH
HHHCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHCCC
PDISILRTEEDVEQTALLQADILHNLWPQLKVGGYLLYVTCSILKQENVEQMQDFMTHYN
CCCEEEECHHHHHHHHHHHHHHHHHHCCCHHHCCEEEHHHHHHHHHCCHHHHHHHHHHCC
NVAAVEFSDGCNWGIEQAIGRQCLPIDSKSGDGFYYALLQKTAE
CEEEEEECCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA