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The map label for this gene is thrC [H]

Identifier: 93007289

GI number: 93007289

Start: 3045616

End: 3047037

Strand: Reverse

Name: thrC [H]

Synonym: Pcryo_2465

Alternate gene names: 93007289

Gene position: 3047037-3045616 (Counterclockwise)

Preceding gene: 93007290

Following gene: 93007288

Centisome position: 99.58

GC content: 43.6

Gene sequence:

>1422_bases
ATGAAATATATCAGTACCCGTGGTCAGACAGCGCCGATGGATTTTAGTGATGTGTTATTAATGGGTCTTGCACCAGACGG
TGGTTTGATGTTGCCTGAGCATTATCCGAATATTGATAGTGCGACGCTTACTGAGTGGCGCGCGCTGAGCTATCCACAGC
TTGCGATGGCGATTATGCAGCGCTTTGCGACTGATATTCCTGCGACTGATTTGCAGGATATTATTGAGCGCACTTATACC
GCTGATATATTTGGCTCTGATGAGATTGTTCCTGTTCGCAAGCTTGAAGATAATATATATATTCTAGGCTTATCAAACGG
TCCGACATTGGCATTTAAAGATGTTGCTATGCAGTTTTTGGGCAATGCTTTCGAATATGTGCTTAAAAGAAAAGACGCGC
GTATTACTATTATTGGTGCAACCAGTGGCGATACGGGCAGTGCGGCAGAATATGCACTACGCGGCAAAGAGAATATCGAA
GTCTTTATGCTTTCACCGCACGGCAAAATGAGTGAATTCCAGCGCGCGCAGATGTATAGCCTGACTGATAAAAATATCCA
TAATATTGCCATCGACGGTATGTTTGATGATTGCCAAGATATTGTCAAAGCGCTACAACAAGATGCAGAATTTAAAGCAG
CTTATAACTTAGGCACGGTTAACTCTATTAACTGGGGTCGTATCCTAGCTCAGATTGTTTACTACTTTAAAGCTTACTTT
GCCGTGACTTCAAGTAACGACGAAAAAGTTAGTTTTAGTGTGCCATCAGGCAACTTTGGTAATATTTGTGCTGGTCATAT
TGCACGTGAAATGGGGCTGCCAGTTAATCGTCTAATCGTCGCAACCAACGAAAACGATGTACTGAACGACTTCTTTAATC
AAGGTGCTTATCAGCCACGTCCGACTGCCAAAACCTATATTACTTCAAGTCCTTCAATGGATATCTCTAAAGCCTCTAAC
TTTGAGCGTTTTGTTTATTTGCTATTAGATAAAGATAGCGCGCGCGTCCATGAACTATTTGAAGGTGTGAAATCTGGTCA
AGGTTTCGATCTATCTGAGCTAATGGAAGCAGTTAATACTCGCTATGGTTTCGCTGCAGGTAAGTCAACCCATAATGACC
GCCTGCAAACCATTAAAGCGCTTTATGAGCAGCATGGAGAGCTGGTTGATCCGCACACCGCTGATGGTATCAAAGTTGCT
AAAGAATTACAGCGCGAAGGTGAAGTGATTGTTTGTGCTGAAACAGCGTTACCGGTTAAGTTTGCCGATACGATTGTTGA
AGCAATAGGTCAAATAGATATTGCTCGTCCAGCCCATACCGAAGGTTTAGAGAGCTTACCGCAACATGTGGTGGTGCTTG
ATAATGAGGCTGAGTTGGTTGCTGAGCAAATCCGTCAACAGGTCACGCCAAACCCTGCCTAG

Upstream 100 bases:

>100_bases
TTGATAGCAAGAGCGGTGATGGTTTTTATTATGCCTTGCTGCAGAAAACTGCTGAATAAACCGTTTCAACTAATAATCTC
ATTGTAGACAGGACAATCTA

Downstream 100 bases:

>100_bases
TCGATTTTATTTTTAGTTAATTGTTAAAATGAATATCCAGTCTAAGGTTACGCTATTGTAAAAAAGTGTTTATGATTGTG
TTATGGTGGATATAATTGTA

Product: threonine synthase

Products: NA

Alternate protein names: TS [H]

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MKYISTRGQTAPMDFSDVLLMGLAPDGGLMLPEHYPNIDSATLTEWRALSYPQLAMAIMQRFATDIPATDLQDIIERTYT
ADIFGSDEIVPVRKLEDNIYILGLSNGPTLAFKDVAMQFLGNAFEYVLKRKDARITIIGATSGDTGSAAEYALRGKENIE
VFMLSPHGKMSEFQRAQMYSLTDKNIHNIAIDGMFDDCQDIVKALQQDAEFKAAYNLGTVNSINWGRILAQIVYYFKAYF
AVTSSNDEKVSFSVPSGNFGNICAGHIAREMGLPVNRLIVATNENDVLNDFFNQGAYQPRPTAKTYITSSPSMDISKASN
FERFVYLLLDKDSARVHELFEGVKSGQGFDLSELMEAVNTRYGFAAGKSTHNDRLQTIKALYEQHGELVDPHTADGIKVA
KELQREGEVIVCAETALPVKFADTIVEAIGQIDIARPAHTEGLESLPQHVVVLDNEAELVAEQIRQQVTPNPA

Sequences:

>Translated_473_residues
MKYISTRGQTAPMDFSDVLLMGLAPDGGLMLPEHYPNIDSATLTEWRALSYPQLAMAIMQRFATDIPATDLQDIIERTYT
ADIFGSDEIVPVRKLEDNIYILGLSNGPTLAFKDVAMQFLGNAFEYVLKRKDARITIIGATSGDTGSAAEYALRGKENIE
VFMLSPHGKMSEFQRAQMYSLTDKNIHNIAIDGMFDDCQDIVKALQQDAEFKAAYNLGTVNSINWGRILAQIVYYFKAYF
AVTSSNDEKVSFSVPSGNFGNICAGHIAREMGLPVNRLIVATNENDVLNDFFNQGAYQPRPTAKTYITSSPSMDISKASN
FERFVYLLLDKDSARVHELFEGVKSGQGFDLSELMEAVNTRYGFAAGKSTHNDRLQTIKALYEQHGELVDPHTADGIKVA
KELQREGEVIVCAETALPVKFADTIVEAIGQIDIARPAHTEGLESLPQHVVVLDNEAELVAEQIRQQVTPNPA
>Mature_473_residues
MKYISTRGQTAPMDFSDVLLMGLAPDGGLMLPEHYPNIDSATLTEWRALSYPQLAMAIMQRFATDIPATDLQDIIERTYT
ADIFGSDEIVPVRKLEDNIYILGLSNGPTLAFKDVAMQFLGNAFEYVLKRKDARITIIGATSGDTGSAAEYALRGKENIE
VFMLSPHGKMSEFQRAQMYSLTDKNIHNIAIDGMFDDCQDIVKALQQDAEFKAAYNLGTVNSINWGRILAQIVYYFKAYF
AVTSSNDEKVSFSVPSGNFGNICAGHIAREMGLPVNRLIVATNENDVLNDFFNQGAYQPRPTAKTYITSSPSMDISKASN
FERFVYLLLDKDSARVHELFEGVKSGQGFDLSELMEAVNTRYGFAAGKSTHNDRLQTIKALYEQHGELVDPHTADGIKVA
KELQREGEVIVCAETALPVKFADTIVEAIGQIDIARPAHTEGLESLPQHVVVLDNEAELVAEQIRQQVTPNPA

Specific function: Catalyzes the gamma-elimination of phosphate from L- phosphohomoserine and the beta-addition of water to produce L- threonine [H]

COG id: COG0498

COG function: function code E; Threonine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine synthase family [H]

Homologues:

Organism=Homo sapiens, GI149193323, Length=443, Percent_Identity=32.7313769751693, Blast_Score=249, Evalue=5e-66,
Organism=Homo sapiens, GI153792148, Length=460, Percent_Identity=34.5652173913044, Blast_Score=241, Evalue=2e-63,
Organism=Escherichia coli, GI1786185, Length=432, Percent_Identity=33.1018518518519, Blast_Score=203, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6319901, Length=463, Percent_Identity=42.1166306695464, Blast_Score=345, Evalue=1e-95,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR004450 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.3.1 [H]

Molecular weight: Translated: 52303; Mature: 52303

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYISTRGQTAPMDFSDVLLMGLAPDGGLMLPEHYPNIDSATLTEWRALSYPQLAMAIMQ
CCCCCCCCCCCCCCHHHEEEEEECCCCCEECCCCCCCCCCCHHHHHHHCCCHHHHHHHHH
RFATDIPATDLQDIIERTYTADIFGSDEIVPVRKLEDNIYILGLSNGPTLAFKDVAMQFL
HHHCCCCCHHHHHHHHHHHHHEECCCCCEEEHEECCCCEEEEEECCCCCCHHHHHHHHHH
GNAFEYVLKRKDARITIIGATSGDTGSAAEYALRGKENIEVFMLSPHGKMSEFQRAQMYS
HHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHH
LTDKNIHNIAIDGMFDDCQDIVKALQQDAEFKAAYNLGTVNSINWGRILAQIVYYFKAYF
CCCCCCCEEEECCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
AVTSSNDEKVSFSVPSGNFGNICAGHIAREMGLPVNRLIVATNENDVLNDFFNQGAYQPR
EEECCCCCEEEEECCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHCCCCCCC
PTAKTYITSSPSMDISKASNFERFVYLLLDKDSARVHELFEGVKSGQGFDLSELMEAVNT
CCCCEEEECCCCCCHHHCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHH
RYGFAAGKSTHNDRLQTIKALYEQHGELVDPHTADGIKVAKELQREGEVIVCAETALPVK
HCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCHH
FADTIVEAIGQIDIARPAHTEGLESLPQHVVVLDNEAELVAEQIRQQVTPNPA
HHHHHHHHHCCCCCCCCCCHHHHHHCCCEEEEECCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKYISTRGQTAPMDFSDVLLMGLAPDGGLMLPEHYPNIDSATLTEWRALSYPQLAMAIMQ
CCCCCCCCCCCCCCHHHEEEEEECCCCCEECCCCCCCCCCCHHHHHHHCCCHHHHHHHHH
RFATDIPATDLQDIIERTYTADIFGSDEIVPVRKLEDNIYILGLSNGPTLAFKDVAMQFL
HHHCCCCCHHHHHHHHHHHHHEECCCCCEEEHEECCCCEEEEEECCCCCCHHHHHHHHHH
GNAFEYVLKRKDARITIIGATSGDTGSAAEYALRGKENIEVFMLSPHGKMSEFQRAQMYS
HHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHH
LTDKNIHNIAIDGMFDDCQDIVKALQQDAEFKAAYNLGTVNSINWGRILAQIVYYFKAYF
CCCCCCCEEEECCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
AVTSSNDEKVSFSVPSGNFGNICAGHIAREMGLPVNRLIVATNENDVLNDFFNQGAYQPR
EEECCCCCEEEEECCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHCCCCCCC
PTAKTYITSSPSMDISKASNFERFVYLLLDKDSARVHELFEGVKSGQGFDLSELMEAVNT
CCCCEEEECCCCCCHHHCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHH
RYGFAAGKSTHNDRLQTIKALYEQHGELVDPHTADGIKVAKELQREGEVIVCAETALPVK
HCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCHH
FADTIVEAIGQIDIARPAHTEGLESLPQHVVVLDNEAELVAEQIRQQVTPNPA
HHHHHHHHHCCCCCCCCCCHHHHHHCCCEEEEECCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8117070 [H]