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The map label for this gene is hisH [H]

Identifier: 93007266

GI number: 93007266

Start: 3017184

End: 3017828

Strand: Reverse

Name: hisH [H]

Synonym: Pcryo_2442

Alternate gene names: 93007266

Gene position: 3017828-3017184 (Counterclockwise)

Preceding gene: 93007267

Following gene: 93007265

Centisome position: 98.63

GC content: 45.12

Gene sequence:

>645_bases
ATGAGTCGTGCCACAAAAATTGCACTATTAGATTATGGTATGGGTAACTTGCACTCAGCCAGCAAAGCATTATCAGCGGT
CGGGGCAGAAGTCTCTATCACCAATGACCCAAAAGTCGTGGCGGCAGCAGATAAGATTTTCTTCCCAGGCGTCGGTGCTA
TGCGTGATTGTATCGCTGGCATGCATGCGGCAGGTATTGACGAGGTCATACGTCAGGCGATATTTAATAAGCCTGTTATG
GCTATCTGTGTTGGCATGCAGGCGTTATTTGAGCAGTCTGCTGAAAATGGTGGCACGCCTTGTTTGAGCATCTTAGATGG
CACGGTACCCGCTTTTGATCCGGCATGGAAAGATGAAAAAGGGGTACAAATAAAAGTACCGCATATGGGCTGGAATACTA
TTAGTGGCATGGATTTTGAGCATCCATTGTGGAGTGGTATCGAAGACAAGGCGCATTTTTATTTTGTGCACAGTTATTAC
TGTGAGCCAGCAGATAGCTCGCAAGTGGCTGCTATTTGTGATTATGGTCAGCCGTTTTGTGCCAGTATCTTGCAAGATAA
TTTATTTGCCACCCAGTTCCATCCAGAAAAAAGCCATACCGCAGGTTTGCAGCTATTAAAGAATTTTGTAGACTGGGATA
TTTAA

Upstream 100 bases:

>100_bases
CGAATCTACCTTTAAAGCCTTTGCTCGTGCGCTACGTATGGCGTGTGAATACGATGAACGTGCGCTAAATACCTTGCCAT
CGACCAAAGAGGCGTTTTAG

Downstream 100 bases:

>100_bases
GAATAAGTATGACATATTTGGTTGCGCAGTCAGGTTGGCAAAGGCACAATCTGAGCGAGGAAGGAGCACAGTACTGCTTT
GCTCTCGCGCAAAAGAGTTT

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MSRATKIALLDYGMGNLHSASKALSAVGAEVSITNDPKVVAAADKIFFPGVGAMRDCIAGMHAAGIDEVIRQAIFNKPVM
AICVGMQALFEQSAENGGTPCLSILDGTVPAFDPAWKDEKGVQIKVPHMGWNTISGMDFEHPLWSGIEDKAHFYFVHSYY
CEPADSSQVAAICDYGQPFCASILQDNLFATQFHPEKSHTAGLQLLKNFVDWDI

Sequences:

>Translated_214_residues
MSRATKIALLDYGMGNLHSASKALSAVGAEVSITNDPKVVAAADKIFFPGVGAMRDCIAGMHAAGIDEVIRQAIFNKPVM
AICVGMQALFEQSAENGGTPCLSILDGTVPAFDPAWKDEKGVQIKVPHMGWNTISGMDFEHPLWSGIEDKAHFYFVHSYY
CEPADSSQVAAICDYGQPFCASILQDNLFATQFHPEKSHTAGLQLLKNFVDWDI
>Mature_213_residues
SRATKIALLDYGMGNLHSASKALSAVGAEVSITNDPKVVAAADKIFFPGVGAMRDCIAGMHAAGIDEVIRQAIFNKPVMA
ICVGMQALFEQSAENGGTPCLSILDGTVPAFDPAWKDEKGVQIKVPHMGWNTISGMDFEHPLWSGIEDKAHFYFVHSYYC
EPADSSQVAAICDYGQPFCASILQDNLFATQFHPEKSHTAGLQLLKNFVDWDI

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=212, Percent_Identity=39.1509433962264, Blast_Score=149, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6319725, Length=216, Percent_Identity=32.4074074074074, Blast_Score=112, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 23199; Mature: 23067

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRATKIALLDYGMGNLHSASKALSAVGAEVSITNDPKVVAAADKIFFPGVGAMRDCIAG
CCCCEEEEEEECCCCCHHHHHHHHHHHCCEEEECCCCCEEEEECCEECCCCHHHHHHHHH
MHAAGIDEVIRQAIFNKPVMAICVGMQALFEQSAENGGTPCLSILDGTVPAFDPAWKDEK
HHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCC
GVQIKVPHMGWNTISGMDFEHPLWSGIEDKAHFYFVHSYYCEPADSSQVAAICDYGQPFC
CCEEEECCCCCCCCCCCCCCCHHHCCCCCCCEEEEEEEEECCCCCCCCEEEEECCCCHHH
ASILQDNLFATQFHPEKSHTAGLQLLKNFVDWDI
HHHHHCCCEEEEECCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SRATKIALLDYGMGNLHSASKALSAVGAEVSITNDPKVVAAADKIFFPGVGAMRDCIAG
CCCEEEEEEECCCCCHHHHHHHHHHHCCEEEECCCCCEEEEECCEECCCCHHHHHHHHH
MHAAGIDEVIRQAIFNKPVMAICVGMQALFEQSAENGGTPCLSILDGTVPAFDPAWKDEK
HHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCC
GVQIKVPHMGWNTISGMDFEHPLWSGIEDKAHFYFVHSYYCEPADSSQVAAICDYGQPFC
CCEEEECCCCCCCCCCCCCCCHHHCCCCCCCEEEEEEEEECCCCCCCCEEEEECCCCHHH
ASILQDNLFATQFHPEKSHTAGLQLLKNFVDWDI
HHHHHCCCEEEEECCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA