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The map label for this gene is potD [H]

Identifier: 93006986

GI number: 93006986

Start: 2648594

End: 2649718

Strand: Direct

Name: potD [H]

Synonym: Pcryo_2162

Alternate gene names: 93006986

Gene position: 2648594-2649718 (Clockwise)

Preceding gene: 93006983

Following gene: 93006998

Centisome position: 86.56

GC content: 43.02

Gene sequence:

>1125_bases
TTGTCCCATTTATCTTCATCCAATTGGTCTACTCACGCCTCTATTAAAATACTGCCGAAAGCATTACTCTGTGCCGCCAT
CGGGCTCTCTATTACCAGCTGTAGCAAATCTGATAACGCGCCTATCGATAGTTCTGCTACCAGCGATGTCACGTTGAACC
TTTATAACTGGTCAGAGTATATGCCGCAAGAAATACTTGACGGCTTTACTGAAGAAACTGGTATCAAAGTCAATTACACC
ACCTTTGACTCTAATGAAGCCATGTATGCCAAGCTAAAGCTGCTTGATGATTCTAGCCAATACGATTTGGCGATTCCATC
GACGTATTATGTCGAAAAAATGGCAAAAGAAGGTCTGCTACAAGAGCTTGATAAGTCTAAATTGAGCAATTTTAAAAACC
TTGACACTTCTTTTACCAATACCAAAGTTGACCCAGAAAACAAATACTCGATTCCTTATATGTGGGGCAGCACCGGTCTT
GCTATCAATGGCGAGACGGTTGATCCAGCCACGGTCAATAGCTGGAATGATTTATGGCGTCCTGAGTATAAGGGTCAAGT
GATGCTGATGAATGATATGCGCGAAGTGTTCGGCATGGCGCTATTAACCCTAGGCTACTCTGGCAACAGTAGAAATCCTG
ACGAAATTAAAGCCGCTTATGAGAAGCTCACGACCTTGATGCCGAATGTAAAAACCTTTAACTCAGATGCTTCACGTATG
CCATATATGGAAGGTGAAACCAGCGTCGGTATGACGTGGAATGGGGAAGCTATCATCGCCAATAGTGAAGGTCTCACTAG
CTTGGTGTATAAATACCCAACCGAAGGTGCGATATTATGGATGGACAATTTTGTCATTCCTAAAAACGCCACGCAAGTCG
ATGCAGCCCACAAGTTCATAGACTATCTGCTACGTCCTGAGAACTCCAAAATTGTTAGTGAAGAGATAGGTTATGCGTCG
CCTAATATTGCGGCACGTGAAATGATGCCAGATGACGTCAAAAATAATCCAACCATCTACCCTAGTAAAGACGTATTGGC
AAAAGCTGAATTCCAAGAAGATGTTGGTGATGAAGCATTGCAGGTGTACCAACAGTATTGGGATAAGCTAAAAACTGGTC
GTTAA

Upstream 100 bases:

>100_bases
TAAATTTCGACTTACCATGATCATAACCGTATAATGAATTAATTTTATGACGGATCATGCGCCCGCTTTTCACCTTTTGC
CCTAATTTAGGAGAAACATT

Downstream 100 bases:

>100_bases
TCATTGTTTATTAATGGTCGATTTTTGATAGCCGTTTTTAGTAATTATGATTGTAAAAAAAAGCTGACTTTATAGAGTCA
GCTTTTTTTATTTATAAATG

Product: extracellular solute-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 374; Mature: 373

Protein sequence:

>374_residues
MSHLSSSNWSTHASIKILPKALLCAAIGLSITSCSKSDNAPIDSSATSDVTLNLYNWSEYMPQEILDGFTEETGIKVNYT
TFDSNEAMYAKLKLLDDSSQYDLAIPSTYYVEKMAKEGLLQELDKSKLSNFKNLDTSFTNTKVDPENKYSIPYMWGSTGL
AINGETVDPATVNSWNDLWRPEYKGQVMLMNDMREVFGMALLTLGYSGNSRNPDEIKAAYEKLTTLMPNVKTFNSDASRM
PYMEGETSVGMTWNGEAIIANSEGLTSLVYKYPTEGAILWMDNFVIPKNATQVDAAHKFIDYLLRPENSKIVSEEIGYAS
PNIAAREMMPDDVKNNPTIYPSKDVLAKAEFQEDVGDEALQVYQQYWDKLKTGR

Sequences:

>Translated_374_residues
MSHLSSSNWSTHASIKILPKALLCAAIGLSITSCSKSDNAPIDSSATSDVTLNLYNWSEYMPQEILDGFTEETGIKVNYT
TFDSNEAMYAKLKLLDDSSQYDLAIPSTYYVEKMAKEGLLQELDKSKLSNFKNLDTSFTNTKVDPENKYSIPYMWGSTGL
AINGETVDPATVNSWNDLWRPEYKGQVMLMNDMREVFGMALLTLGYSGNSRNPDEIKAAYEKLTTLMPNVKTFNSDASRM
PYMEGETSVGMTWNGEAIIANSEGLTSLVYKYPTEGAILWMDNFVIPKNATQVDAAHKFIDYLLRPENSKIVSEEIGYAS
PNIAAREMMPDDVKNNPTIYPSKDVLAKAEFQEDVGDEALQVYQQYWDKLKTGR
>Mature_373_residues
SHLSSSNWSTHASIKILPKALLCAAIGLSITSCSKSDNAPIDSSATSDVTLNLYNWSEYMPQEILDGFTEETGIKVNYTT
FDSNEAMYAKLKLLDDSSQYDLAIPSTYYVEKMAKEGLLQELDKSKLSNFKNLDTSFTNTKVDPENKYSIPYMWGSTGLA
INGETVDPATVNSWNDLWRPEYKGQVMLMNDMREVFGMALLTLGYSGNSRNPDEIKAAYEKLTTLMPNVKTFNSDASRMP
YMEGETSVGMTWNGEAIIANSEGLTSLVYKYPTEGAILWMDNFVIPKNATQVDAAHKFIDYLLRPENSKIVSEEIGYASP
NIAAREMMPDDVKNNPTIYPSKDVLAKAEFQEDVGDEALQVYQQYWDKLKTGR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=331, Percent_Identity=53.1722054380665, Blast_Score=368, Evalue=1e-103,
Organism=Escherichia coli, GI1787078, Length=352, Percent_Identity=34.6590909090909, Blast_Score=198, Evalue=4e-52,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 41873; Mature: 41742

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHLSSSNWSTHASIKILPKALLCAAIGLSITSCSKSDNAPIDSSATSDVTLNLYNWSEY
CCCCCCCCCCCCEEEEEHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCEEEEEECCHHC
MPQEILDGFTEETGIKVNYTTFDSNEAMYAKLKLLDDSSQYDLAIPSTYYVEKMAKEGLL
CHHHHHCCCHHCCCCEEEEEEECCCCEEEEEEEEECCCCCCEEECCCHHHHHHHHHHHHH
QELDKSKLSNFKNLDTSFTNTKVDPENKYSIPYMWGSTGLAINGETVDPATVNSWNDLWR
HHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCEEECCCCEEEECCCCCCCCCCCCHHHCC
PEYKGQVMLMNDMREVFGMALLTLGYSGNSRNPDEIKAAYEKLTTLMPNVKTFNSDASRM
CCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHCC
PYMEGETSVGMTWNGEAIIANSEGLTSLVYKYPTEGAILWMDNFVIPKNATQVDAAHKFI
CCCCCCCCCCEEECCCEEEECCCCCEEHHEECCCCCEEEEECCEECCCCCCHHHHHHHHH
DYLLRPENSKIVSEEIGYASPNIAAREMMPDDVKNNPTIYPSKDVLAKAEFQEDVGDEAL
HHHHCCCCCCCHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHH
QVYQQYWDKLKTGR
HHHHHHHHHHCCCC
>Mature Secondary Structure 
SHLSSSNWSTHASIKILPKALLCAAIGLSITSCSKSDNAPIDSSATSDVTLNLYNWSEY
CCCCCCCCCCCEEEEEHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCEEEEEECCHHC
MPQEILDGFTEETGIKVNYTTFDSNEAMYAKLKLLDDSSQYDLAIPSTYYVEKMAKEGLL
CHHHHHCCCHHCCCCEEEEEEECCCCEEEEEEEEECCCCCCEEECCCHHHHHHHHHHHHH
QELDKSKLSNFKNLDTSFTNTKVDPENKYSIPYMWGSTGLAINGETVDPATVNSWNDLWR
HHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCEEECCCCEEEECCCCCCCCCCCCHHHCC
PEYKGQVMLMNDMREVFGMALLTLGYSGNSRNPDEIKAAYEKLTTLMPNVKTFNSDASRM
CCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHCC
PYMEGETSVGMTWNGEAIIANSEGLTSLVYKYPTEGAILWMDNFVIPKNATQVDAAHKFI
CCCCCCCCCCEEECCCEEEECCCCCEEHHEECCCCCEEEEECCEECCCCCCHHHHHHHHH
DYLLRPENSKIVSEEIGYASPNIAAREMMPDDVKNNPTIYPSKDVLAKAEFQEDVGDEAL
HHHHCCCCCCCHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHH
QVYQQYWDKLKTGR
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]