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The map label for this gene is trmB [H]

Identifier: 93006837

GI number: 93006837

Start: 2475925

End: 2476746

Strand: Direct

Name: trmB [H]

Synonym: Pcryo_2013

Alternate gene names: 93006837

Gene position: 2475925-2476746 (Clockwise)

Preceding gene: 93006835

Following gene: 93006838

Centisome position: 80.92

GC content: 46.11

Gene sequence:

>822_bases
ATGAGTCAACATCCTGATATTAATACCAACGTCGACGCCACCAGCCTTACCGATGATCAAAAATCTTTAGACACTAATGC
TACTAGTGGCAATGAGGTCGCACCTGACGGTAATAAGCATATTCGTATCGTCAACACCTTTATGAAACGCCGTACGCACA
TGAATAAAAATGCCGAGCTGGCATTGACGGCGCCTGAATTTGCGCATTACTTGGTTAATAACAGCTTTGGTGATGGTAAT
CTTGAAGGTATTGACGATTTGCGCGCGCTATTTGCTGAATTGCCAAATGGCAGTGAGGCACCCTTGACCCTTGAGATTGG
TTTTGGACTTGGGGATTCGTTCATTGAAATGGCAGCAGCCGAGCCAAGTCGTAATTTTGTTGGCATTGAAGTCCATGAGC
CGGGTATCGGCAAATGCGCTTATATGGCTGGTACTCAAAATTTGAGTAATGTCAGAATCATCAATGGTGATGCCATTCAA
CTACTCAAACAGCTACCAGAAAACCATATCGATCGCATTCAGCTCTACTTTCCTGACCCATGGCAAAAAAAGCGCCATCA
TAAACGCCGCTTTGTTAGCCCTGAGCGTATGGCGATTGTCACCCGCAGCCTAAAGCAAGGCGGCTGGTTCCATACCGCCA
CTGACTGGGAACATTATGCCTTTTGGATGGTAGAAGTTTTGGATGGGTTTGTAGGCTTAAGCAATCAAGCAGGCGCAGGC
AATTTCACTTCCCGCCCTGACTTTCGTCCAATGACAAAATTTGAGCGCCGCGGTCTAGCAAGCGGACATGGGGTTTGGGA
TTTAATCTATTTAAAAGACTAA

Upstream 100 bases:

>100_bases
AAAACGCGCTAACATTATTAAAAAAGTATTGTTCATTCACAGCCGTACAGATTTCACTTATAATAAGCGCACTACCCATT
TATTAAGCCTTGTTAATCCT

Downstream 100 bases:

>100_bases
TACCAAACGCATTACGTCGGCTTCCTTTAAAGCGGCTAAGATATTTATCAGTATCTTAGCCGCTTTTTTATATCTATCAG
TCTTCAAACCTTTTACTCAT

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MSQHPDINTNVDATSLTDDQKSLDTNATSGNEVAPDGNKHIRIVNTFMKRRTHMNKNAELALTAPEFAHYLVNNSFGDGN
LEGIDDLRALFAELPNGSEAPLTLEIGFGLGDSFIEMAAAEPSRNFVGIEVHEPGIGKCAYMAGTQNLSNVRIINGDAIQ
LLKQLPENHIDRIQLYFPDPWQKKRHHKRRFVSPERMAIVTRSLKQGGWFHTATDWEHYAFWMVEVLDGFVGLSNQAGAG
NFTSRPDFRPMTKFERRGLASGHGVWDLIYLKD

Sequences:

>Translated_273_residues
MSQHPDINTNVDATSLTDDQKSLDTNATSGNEVAPDGNKHIRIVNTFMKRRTHMNKNAELALTAPEFAHYLVNNSFGDGN
LEGIDDLRALFAELPNGSEAPLTLEIGFGLGDSFIEMAAAEPSRNFVGIEVHEPGIGKCAYMAGTQNLSNVRIINGDAIQ
LLKQLPENHIDRIQLYFPDPWQKKRHHKRRFVSPERMAIVTRSLKQGGWFHTATDWEHYAFWMVEVLDGFVGLSNQAGAG
NFTSRPDFRPMTKFERRGLASGHGVWDLIYLKD
>Mature_272_residues
SQHPDINTNVDATSLTDDQKSLDTNATSGNEVAPDGNKHIRIVNTFMKRRTHMNKNAELALTAPEFAHYLVNNSFGDGNL
EGIDDLRALFAELPNGSEAPLTLEIGFGLGDSFIEMAAAEPSRNFVGIEVHEPGIGKCAYMAGTQNLSNVRIINGDAIQL
LKQLPENHIDRIQLYFPDPWQKKRHHKRRFVSPERMAIVTRSLKQGGWFHTATDWEHYAFWMVEVLDGFVGLSNQAGAGN
FTSRPDFRPMTKFERRGLASGHGVWDLIYLKD

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=236, Percent_Identity=42.3728813559322, Blast_Score=190, Evalue=7e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 30593; Mature: 30462

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQHPDINTNVDATSLTDDQKSLDTNATSGNEVAPDGNKHIRIVNTFMKRRTHMNKNAEL
CCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCCEE
ALTAPEFAHYLVNNSFGDGNLEGIDDLRALFAELPNGSEAPLTLEIGFGLGDSFIEMAAA
EEECHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHC
EPSRNFVGIEVHEPGIGKCAYMAGTQNLSNVRIINGDAIQLLKQLPENHIDRIQLYFPDP
CCCCCEEEEEEECCCCCCEEEEECCCCCCEEEEECCHHHHHHHHCCHHCCCEEEEEECCH
WQKKRHHKRRFVSPERMAIVTRSLKQGGWFHTATDWEHYAFWMVEVLDGFVGLSNQAGAG
HHHHHHHHHHCCCHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHCCCCCCCCC
NFTSRPDFRPMTKFERRGLASGHGVWDLIYLKD
CCCCCCCCCCHHHHHHCCCCCCCCEEEEEEECC
>Mature Secondary Structure 
SQHPDINTNVDATSLTDDQKSLDTNATSGNEVAPDGNKHIRIVNTFMKRRTHMNKNAEL
CCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCCEE
ALTAPEFAHYLVNNSFGDGNLEGIDDLRALFAELPNGSEAPLTLEIGFGLGDSFIEMAAA
EEECHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHC
EPSRNFVGIEVHEPGIGKCAYMAGTQNLSNVRIINGDAIQLLKQLPENHIDRIQLYFPDP
CCCCCEEEEEEECCCCCCEEEEECCCCCCEEEEECCHHHHHHHHCCHHCCCEEEEEECCH
WQKKRHHKRRFVSPERMAIVTRSLKQGGWFHTATDWEHYAFWMVEVLDGFVGLSNQAGAG
HHHHHHHHHHCCCHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHCCCCCCCCC
NFTSRPDFRPMTKFERRGLASGHGVWDLIYLKD
CCCCCCCCCCHHHHHHCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA