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The map label for this gene is rnt [H]

Identifier: 93006690

GI number: 93006690

Start: 2304990

End: 2305730

Strand: Direct

Name: rnt [H]

Synonym: Pcryo_1866

Alternate gene names: 93006690

Gene position: 2304990-2305730 (Clockwise)

Preceding gene: 93006689

Following gene: 93006703

Centisome position: 75.33

GC content: 43.05

Gene sequence:

>741_bases
ATGACCATTAAAAAAGATACCGCTTTACCCAAAATAGATGTAGCCGTTCCTATAGAGTCGACGACAGAAACGCTAGCACC
CCTGAAACTAAAAGAGCGTTTTCGGAAATTCCTACCAGTAGTAGTGGATGTTGAAACGGCAGGATTTAACGCGCAAACTG
ATGCCTTGTTAGAGATTGCCTGTATTCCTGTACTACTGAATGAGCAGGGTGTTTTTTATCCTGGTGAGGCATTTAATGCT
CATATCGAACCTTTTGAAGGGGCAAATCTTGAGCCAAGTGCCCTCGCCTTTACCGGCATTGACCCTAACAATCCGCTGCG
TAAAGCCATTGCTGAAGACGAAAAAACGGCGCTACGTCGTATTTTTAAAGGGTTAAAAGAAGTACGCCGTATTGAAGACT
GCCGTCAATGTGTGTTAATCGGACATAATGCACATTTTGATTTGGCATTTTTAAATGCCGCGGTATTACGTACCAATAAT
AAGAGCCACAATCCTTTCCATAACTTCTCAGTCTTTGATACGGTGACTTTATCAGCACTTGCCTTTGGTCAAACGGTGCT
AGCACGTGCCTGTAAAGCCGCAGGACTTGATTTTGATGGTAACGACGCTCATTCTGCACTCTACGACACGCAAAAAACCG
CTGAGCTGTTTTGTTATATCTTAAATAATTATCCTATGCTCGCTAATGCCATGCACAGCGAGCCTGAAATCACAGAAACT
GCGACTGATAATAGCAAATAA

Upstream 100 bases:

>100_bases
GTAATGCTATCTGTCCATTGAAAAATAGTATGAGTGTCATAGGAAGTTTATTTTTTTATTAAACACAATAGCCCGACTGA
TAAAAGCGATTTAACCTGAT

Downstream 100 bases:

>100_bases
ATCAGTTATTACAGAGTACGTGTTGCTCGCTACCCATAATAGTGCTATTTGTAAGGTCTGCTAACTTTAACCTTACAATT
TAACAGGACGATTTAATCAT

Product: ribonuclease T

Products: NA

Alternate protein names: Exoribonuclease T; RNase T [H]

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MTIKKDTALPKIDVAVPIESTTETLAPLKLKERFRKFLPVVVDVETAGFNAQTDALLEIACIPVLLNEQGVFYPGEAFNA
HIEPFEGANLEPSALAFTGIDPNNPLRKAIAEDEKTALRRIFKGLKEVRRIEDCRQCVLIGHNAHFDLAFLNAAVLRTNN
KSHNPFHNFSVFDTVTLSALAFGQTVLARACKAAGLDFDGNDAHSALYDTQKTAELFCYILNNYPMLANAMHSEPEITET
ATDNSK

Sequences:

>Translated_246_residues
MTIKKDTALPKIDVAVPIESTTETLAPLKLKERFRKFLPVVVDVETAGFNAQTDALLEIACIPVLLNEQGVFYPGEAFNA
HIEPFEGANLEPSALAFTGIDPNNPLRKAIAEDEKTALRRIFKGLKEVRRIEDCRQCVLIGHNAHFDLAFLNAAVLRTNN
KSHNPFHNFSVFDTVTLSALAFGQTVLARACKAAGLDFDGNDAHSALYDTQKTAELFCYILNNYPMLANAMHSEPEITET
ATDNSK
>Mature_245_residues
TIKKDTALPKIDVAVPIESTTETLAPLKLKERFRKFLPVVVDVETAGFNAQTDALLEIACIPVLLNEQGVFYPGEAFNAH
IEPFEGANLEPSALAFTGIDPNNPLRKAIAEDEKTALRRIFKGLKEVRRIEDCRQCVLIGHNAHFDLAFLNAAVLRTNNK
SHNPFHNFSVFDTVTLSALAFGQTVLARACKAAGLDFDGNDAHSALYDTQKTAELFCYILNNYPMLANAMHSEPEITETA
TDNSK

Specific function: Responsible for the end-turnover of tRNA:specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis [H]

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1787941, Length=200, Percent_Identity=54, Blast_Score=221, Evalue=4e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR005987
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00929 Exonuc_X-T [H]

EC number: 3.1.13.-

Molecular weight: Translated: 27121; Mature: 26990

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIKKDTALPKIDVAVPIESTTETLAPLKLKERFRKFLPVVVDVETAGFNAQTDALLEIA
CCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHH
CIPVLLNEQGVFYPGEAFNAHIEPFEGANLEPSALAFTGIDPNNPLRKAIAEDEKTALRR
HEEEEECCCCCEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHCHHHHHHHH
IFKGLKEVRRIEDCRQCVLIGHNAHFDLAFLNAAVLRTNNKSHNPFHNFSVFDTVTLSAL
HHHHHHHHHHHHHHHHHEEECCCCCEEHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
AFGQTVLARACKAAGLDFDGNDAHSALYDTQKTAELFCYILNNYPMLANAMHSEPEITET
HHHHHHHHHHHHHCCCCCCCCCHHHHHHCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCC
ATDNSK
CCCCCC
>Mature Secondary Structure 
TIKKDTALPKIDVAVPIESTTETLAPLKLKERFRKFLPVVVDVETAGFNAQTDALLEIA
CCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHH
CIPVLLNEQGVFYPGEAFNAHIEPFEGANLEPSALAFTGIDPNNPLRKAIAEDEKTALRR
HEEEEECCCCCEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHCHHHHHHHH
IFKGLKEVRRIEDCRQCVLIGHNAHFDLAFLNAAVLRTNNKSHNPFHNFSVFDTVTLSAL
HHHHHHHHHHHHHHHHHEEECCCCCEEHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
AFGQTVLARACKAAGLDFDGNDAHSALYDTQKTAELFCYILNNYPMLANAMHSEPEITET
HHHHHHHHHHHHHCCCCCCCCCHHHHHHCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCC
ATDNSK
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA