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The map label for this gene is iscA [H]

Identifier: 93006482

GI number: 93006482

Start: 2042183

End: 2042503

Strand: Direct

Name: iscA [H]

Synonym: Pcryo_1658

Alternate gene names: 93006482

Gene position: 2042183-2042503 (Clockwise)

Preceding gene: 93006481

Following gene: 93006483

Centisome position: 66.74

GC content: 44.55

Gene sequence:

>321_bases
ATGATTGAAATGACAGAACGCGCCGCTCAGCATGTGCGTGAGTTTTTGGACAATCGCGGTAAAGGCGAAGGCATACGCGT
GGGTATTCGCACGGCAGGATGTTCAGGCTTGGCTTATGTGTTAGAGTTCGTCGACACCCCTGATGAAAACGATACTCGCT
ATGAGAGTCTTGGTGTCAGTATTTTTATTGACCCTAAAAGCTTGGTATATCTCGATGGTCTATTAATGGATTATGAAAAA
GAAGGTCTCAATGAAGGCTTTAAATTCAGTAATCCTAACCAGAAAGGCGAATGTGGCTGCGGTGAGTCTTTTACGGTGTA
A

Upstream 100 bases:

>100_bases
ATGACGCTTATGTAATTGTCACCTTTTATTTATTTCGTTATTTATTTCGTTATTTATTTTGAATTTTTATCCCTATTATT
GATAGTCAATGGGAGTTGGC

Downstream 100 bases:

>100_bases
CCGTTTTTACTATACAAAATAGAAGTCCTTAAAATAATAAATAAAATAAAGGCAGACGATATGACAGACAACGTATTAGA
ACCACAGTTTGATAATTTTT

Product: iron-sulfur cluster assembly protein IscA

Products: NA

Alternate protein names: Iron-sulfur cluster assembly protein [H]

Number of amino acids: Translated: 106; Mature: 106

Protein sequence:

>106_residues
MIEMTERAAQHVREFLDNRGKGEGIRVGIRTAGCSGLAYVLEFVDTPDENDTRYESLGVSIFIDPKSLVYLDGLLMDYEK
EGLNEGFKFSNPNQKGECGCGESFTV

Sequences:

>Translated_106_residues
MIEMTERAAQHVREFLDNRGKGEGIRVGIRTAGCSGLAYVLEFVDTPDENDTRYESLGVSIFIDPKSLVYLDGLLMDYEK
EGLNEGFKFSNPNQKGECGCGESFTV
>Mature_106_residues
MIEMTERAAQHVREFLDNRGKGEGIRVGIRTAGCSGLAYVLEFVDTPDENDTRYESLGVSIFIDPKSLVYLDGLLMDYEK
EGLNEGFKFSNPNQKGECGCGESFTV

Specific function: Is able to transfer iron-sulfur clusters to apo- ferredoxin. Multiple cycles of [2Fe2S] cluster formation and transfer are observed, suggesting that iscA acts catalytically. Recruits intracellular free iron so as to provide iron for the assembly of transi

COG id: COG0316

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesB/iscA family [H]

Homologues:

Organism=Homo sapiens, GI52426768, Length=105, Percent_Identity=40, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI1788877, Length=105, Percent_Identity=60.952380952381, Blast_Score=136, Evalue=3e-34,
Organism=Escherichia coli, GI1787974, Length=105, Percent_Identity=40.9523809523809, Blast_Score=100, Evalue=2e-23,
Organism=Escherichia coli, GI1786351, Length=108, Percent_Identity=42.5925925925926, Blast_Score=74, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI25146411, Length=105, Percent_Identity=37.1428571428571, Blast_Score=89, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI193209025, Length=105, Percent_Identity=37.1428571428571, Blast_Score=89, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6323001, Length=106, Percent_Identity=42.4528301886792, Blast_Score=87, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24642263, Length=105, Percent_Identity=38.0952380952381, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000361
- InterPro:   IPR016092
- InterPro:   IPR017870
- InterPro:   IPR011302 [H]

Pfam domain/function: PF01521 Fe-S_biosyn [H]

EC number: NA

Molecular weight: Translated: 11777; Mature: 11777

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: PS01152 HESB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEMTERAAQHVREFLDNRGKGEGIRVGIRTAGCSGLAYVLEFVDTPDENDTRYESLGVS
CCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCEEECCCEE
IFIDPKSLVYLDGLLMDYEKEGLNEGFKFSNPNQKGECGCGESFTV
EEECCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIEMTERAAQHVREFLDNRGKGEGIRVGIRTAGCSGLAYVLEFVDTPDENDTRYESLGVS
CCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCEEECCCEE
IFIDPKSLVYLDGLLMDYEKEGLNEGFKFSNPNQKGECGCGESFTV
EEECCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]