Click here to switch to the map view.

The map label for this gene is intA [H]

Identifier: 93006429

GI number: 93006429

Start: 1969590

End: 1970936

Strand: Direct

Name: intA [H]

Synonym: Pcryo_1605

Alternate gene names: 93006429

Gene position: 1969590-1970936 (Clockwise)

Preceding gene: 93006428

Following gene: 93006438

Centisome position: 64.37

GC content: 41.5

Gene sequence:

>1347_bases
ATGGAAAGAAAAGCGATCAGTTCAGACCGAAGCATTAGCAGTCATAAACCTGAAGATAAGAAGTACTTCGTTGCTGTCAA
AAATCATCCCAAACTCTTCTTAATGGTGCGTCCTACTGAAACCAAATCATGGATGTATCGCTATTACCCACCTTCAAACC
CCAAACAAAGTATTATCTCCATCGGTATATATCCAAGTATCTCATATGCTCGTGCCTGTGAAGTTTGGCGTGAGTATGAA
GATCTTTTAAGCAAAGGCATTGATCCTAAAATCCACCGTGAAGATATAAAGAAAAGTTTTATTAGTAAAACTAAAAACTC
TTTTAATCACTTTGCTTGGGAATACTTCGATACTCTTGAACAGAGTCAAAAAGGTAATACGCTCATTCGTAAGAAGGGTC
GCTTGGAGCTTATATGCTCCTACATAGGTAATGAGCCAATTAGTGAAATTGGCTCTCCTAGAATGCTTGAAGTGCTTTTA
GATATTCAAGCCAAGTCTTTAAATAAAGACGACAAGCCTACTGACAAAGCTGAACGCTGCGCAGGCATCGCTAGTGATGT
ATTTGTTTATGCTAATGCTCGTGGGTTTTGTAGTAGCAACCCTGCGGCGCTTATTAAAAGCCAATTAGCTAAATCTAGTT
ACGGTCATCGTCCAGCTGTTACCAAGCCGAAAGACTTAGCCAAGTTACTACGAGCTATTGAAACGATAGAGGGTGATCCA
AACACCATTAACTCCCTTAGGCTATTAGCGCTTCTCTTTGTTCGCAATGGTGATTTAAGACGTATGTGCTGGATTGATCT
AGACCTAGAGGCTGGCAGGTGGCGGTTAAAGCCATTGAAAGGACAAGGCAAGGTAAATATGGTCAAGGACATGGTTGTTC
CCCTACCTCGTCAAGCCGTAGCTATATTGCGTGAGCAACAAAAGATTAATGGGCATACCAAGTATGTCTTTTTTAGCCAG
ACGGCAAAAAAGCATCAGATCATATCTGACGCTACTGCTAATAAGCGTTTAAAGGATTTAGGCTATAAAGACATACATTG
TGCTCATGGCTTTAGAGCGACAGCGAAGACCATCCTACAAGAGCAGTTAAAGTATCCGCTTGTTCTTGTTGAGATGGCTT
TAGGTCATACTACTAAAGACCCTAATGGAACTGCTTACGGTCGGTTTGAATACATCGACGATCGTAGCGACATGATGCAG
AAATGGGCAGACTATCTAGATGCTTTACGAGAAGGTAGAGATACGGCAAAGTTTAAAGCAGATGCAGAAGATAAAGCTAA
TCCTACCACCCAGCTGCAAGCATTGATCGCCCAGCTAGGAAAGGATAAGGTTTTAGAGCTATTGTAG

Upstream 100 bases:

>100_bases
CTGGCGTTCTATACTGTCCTATGAATCTATCCTAATCCATGCACTTTGAGTATACTGTCAAGTATACCGAGTTTTGAGTC
TACTCATGGAGACGTAGGAT

Downstream 100 bases:

>100_bases
AAGAATAAATAGCTCATATTAAACTTTCTTTGAAGCACTCACTTTTAAAAGTATTGAATTATATATCATATACGAAAAGG
CTATTTTCTATTTAGAACCA

Product: phage integrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MERKAISSDRSISSHKPEDKKYFVAVKNHPKLFLMVRPTETKSWMYRYYPPSNPKQSIISIGIYPSISYARACEVWREYE
DLLSKGIDPKIHREDIKKSFISKTKNSFNHFAWEYFDTLEQSQKGNTLIRKKGRLELICSYIGNEPISEIGSPRMLEVLL
DIQAKSLNKDDKPTDKAERCAGIASDVFVYANARGFCSSNPAALIKSQLAKSSYGHRPAVTKPKDLAKLLRAIETIEGDP
NTINSLRLLALLFVRNGDLRRMCWIDLDLEAGRWRLKPLKGQGKVNMVKDMVVPLPRQAVAILREQQKINGHTKYVFFSQ
TAKKHQIISDATANKRLKDLGYKDIHCAHGFRATAKTILQEQLKYPLVLVEMALGHTTKDPNGTAYGRFEYIDDRSDMMQ
KWADYLDALREGRDTAKFKADAEDKANPTTQLQALIAQLGKDKVLELL

Sequences:

>Translated_448_residues
MERKAISSDRSISSHKPEDKKYFVAVKNHPKLFLMVRPTETKSWMYRYYPPSNPKQSIISIGIYPSISYARACEVWREYE
DLLSKGIDPKIHREDIKKSFISKTKNSFNHFAWEYFDTLEQSQKGNTLIRKKGRLELICSYIGNEPISEIGSPRMLEVLL
DIQAKSLNKDDKPTDKAERCAGIASDVFVYANARGFCSSNPAALIKSQLAKSSYGHRPAVTKPKDLAKLLRAIETIEGDP
NTINSLRLLALLFVRNGDLRRMCWIDLDLEAGRWRLKPLKGQGKVNMVKDMVVPLPRQAVAILREQQKINGHTKYVFFSQ
TAKKHQIISDATANKRLKDLGYKDIHCAHGFRATAKTILQEQLKYPLVLVEMALGHTTKDPNGTAYGRFEYIDDRSDMMQ
KWADYLDALREGRDTAKFKADAEDKANPTTQLQALIAQLGKDKVLELL
>Mature_448_residues
MERKAISSDRSISSHKPEDKKYFVAVKNHPKLFLMVRPTETKSWMYRYYPPSNPKQSIISIGIYPSISYARACEVWREYE
DLLSKGIDPKIHREDIKKSFISKTKNSFNHFAWEYFDTLEQSQKGNTLIRKKGRLELICSYIGNEPISEIGSPRMLEVLL
DIQAKSLNKDDKPTDKAERCAGIASDVFVYANARGFCSSNPAALIKSQLAKSSYGHRPAVTKPKDLAKLLRAIETIEGDP
NTINSLRLLALLFVRNGDLRRMCWIDLDLEAGRWRLKPLKGQGKVNMVKDMVVPLPRQAVAILREQQKINGHTKYVFFSQ
TAKKHQIISDATANKRLKDLGYKDIHCAHGFRATAKTILQEQLKYPLVLVEMALGHTTKDPNGTAYGRFEYIDDRSDMMQ
KWADYLDALREGRDTAKFKADAEDKANPTTQLQALIAQLGKDKVLELL

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: COG0582

COG function: function code L; Integrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788974, Length=409, Percent_Identity=27.3838630806846, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI145693166, Length=406, Percent_Identity=26.6009852216749, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1788690, Length=406, Percent_Identity=27.5862068965517, Blast_Score=112, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 51041; Mature: 51041

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERKAISSDRSISSHKPEDKKYFVAVKNHPKLFLMVRPTETKSWMYRYYPPSNPKQSIIS
CCCCCCCCCCCCCCCCCCCCEEEEEEECCCEEEEEEECCCCCCCEEEECCCCCCCCCEEE
IGIYPSISYARACEVWREYEDLLSKGIDPKIHREDIKKSFISKTKNSFNHFAWEYFDTLE
EECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSQKGNTLIRKKGRLELICSYIGNEPISEIGSPRMLEVLLDIQAKSLNKDDKPTDKAERC
HHCCCCHHHHCCCCHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHH
AGIASDVFVYANARGFCSSNPAALIKSQLAKSSYGHRPAVTKPKDLAKLLRAIETIEGDP
HCCHHCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCC
NTINSLRLLALLFVRNGDLRRMCWIDLDLEAGRWRLKPLKGQGKVNMVKDMVVPLPRQAV
CHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCEEEEECCCCCCHHHHHHHHCCCCHHHH
AILREQQKINGHTKYVFFSQTAKKHQIISDATANKRLKDLGYKDIHCAHGFRATAKTILQ
HHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHH
EQLKYPLVLVEMALGHTTKDPNGTAYGRFEYIDDRSDMMQKWADYLDALREGRDTAKFKA
HHHCCCCEEEHHHHCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHCCCCCHHEEC
DAEDKANPTTQLQALIAQLGKDKVLELL
CCCCCCCHHHHHHHHHHHHCCHHHHHCC
>Mature Secondary Structure
MERKAISSDRSISSHKPEDKKYFVAVKNHPKLFLMVRPTETKSWMYRYYPPSNPKQSIIS
CCCCCCCCCCCCCCCCCCCCEEEEEEECCCEEEEEEECCCCCCCEEEECCCCCCCCCEEE
IGIYPSISYARACEVWREYEDLLSKGIDPKIHREDIKKSFISKTKNSFNHFAWEYFDTLE
EECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSQKGNTLIRKKGRLELICSYIGNEPISEIGSPRMLEVLLDIQAKSLNKDDKPTDKAERC
HHCCCCHHHHCCCCHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHH
AGIASDVFVYANARGFCSSNPAALIKSQLAKSSYGHRPAVTKPKDLAKLLRAIETIEGDP
HCCHHCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCC
NTINSLRLLALLFVRNGDLRRMCWIDLDLEAGRWRLKPLKGQGKVNMVKDMVVPLPRQAV
CHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCEEEEECCCCCCHHHHHHHHCCCCHHHH
AILREQQKINGHTKYVFFSQTAKKHQIISDATANKRLKDLGYKDIHCAHGFRATAKTILQ
HHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHH
EQLKYPLVLVEMALGHTTKDPNGTAYGRFEYIDDRSDMMQKWADYLDALREGRDTAKFKA
HHHCCCCEEEHHHHCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHCCCCCHHEEC
DAEDKANPTTQLQALIAQLGKDKVLELL
CCCCCCCHHHHHHHHHHHHCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7511583; 9278503; 9205837; 7524073 [H]