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The map label for this gene is truA [H]

Identifier: 93006392

GI number: 93006392

Start: 1924280

End: 1925122

Strand: Direct

Name: truA [H]

Synonym: Pcryo_1568

Alternate gene names: 93006392

Gene position: 1924280-1925122 (Clockwise)

Preceding gene: 93006391

Following gene: 93006393

Centisome position: 62.89

GC content: 45.91

Gene sequence:

>843_bases
ATGACCATATCCATGACTGAAAACAACGAAGCAAAGCGCCCAATTTATACCTTAGCCATTGCTATTGAATTTTTGGGCAC
TCACTATCATGGTTGGCAACGCCAAAGAGAGGTACTTGGCGTACAAGAAGCGTTAGAGACCGCCATTAGTACTGTTGCCA
ATGAACCAGTAGAAGTGATTGCTGCAGGGCGCACAGACTCAAGTGTTCATGCTGGCAATATGATTGCCCATTTTGTTACT
CATGCTTATCGACCCTCGCACAATTGGCTGCGTGGTGTAAATAGTCTTTTGCCTGATGATATCGCTTTACGCTGGGTTCA
GCCGATGCCTGCTGACTTTCATGCTCGCTTTGCCGCTATTGCCCGTCGTTATCGCTACATTACTCTAAACCAAACTCAGC
GTCCTGCTATCCTCAATCATCAGGTGACACATATTCATGAGCCGCTTGATCTAAAAGCCATGCAGCTCGCAGCAGCTGAT
ATCGTTGGTACACATGATTTTAGTAGCTACCGTGCTGCTGCTTGCCAATCCAATCAGCCTGTGCGCCATATTAGCCATGC
CAACCTATTTGCTCATGGTCAGTTTATCGTCTTCGATATTCAAGCTGATGGGTTTTTGCACCATATGGTGCGAAACCTGA
TGGGTACGCTATATGCCATCGGTAGACATGAATTAGAACCAGCTGACTTTTTAAATATCTTGGCTAAAAAGGATCGTACG
ATAGCGCCGCCTACCGCTTCTGGTGATGGACTATATTTTATTAATGCCTATTATCCTGAGCATTTTCAAACCTTACTGCC
AAATGCGCCACTCACCCCTATTTGGCTCAACCTGCCCGATTAG

Upstream 100 bases:

>100_bases
AGCAAGGCGTCGACAACGCTGGTTAAATAACGTCCAGCAAACGGCCACCAGTATCAAAAAACGTTAAACTATCACTCACA
AACTCTGTATAAGTTCCAAA

Downstream 100 bases:

>100_bases
ATTAATATAATCTGGCATACATATCACTTACAGGCTGTCAAGTAAAAATCAGGTTCCGTATTGCTTTAATCTGCTAACTT
ACGTATAATATGGGCTTATT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MTISMTENNEAKRPIYTLAIAIEFLGTHYHGWQRQREVLGVQEALETAISTVANEPVEVIAAGRTDSSVHAGNMIAHFVT
HAYRPSHNWLRGVNSLLPDDIALRWVQPMPADFHARFAAIARRYRYITLNQTQRPAILNHQVTHIHEPLDLKAMQLAAAD
IVGTHDFSSYRAAACQSNQPVRHISHANLFAHGQFIVFDIQADGFLHHMVRNLMGTLYAIGRHELEPADFLNILAKKDRT
IAPPTASGDGLYFINAYYPEHFQTLLPNAPLTPIWLNLPD

Sequences:

>Translated_280_residues
MTISMTENNEAKRPIYTLAIAIEFLGTHYHGWQRQREVLGVQEALETAISTVANEPVEVIAAGRTDSSVHAGNMIAHFVT
HAYRPSHNWLRGVNSLLPDDIALRWVQPMPADFHARFAAIARRYRYITLNQTQRPAILNHQVTHIHEPLDLKAMQLAAAD
IVGTHDFSSYRAAACQSNQPVRHISHANLFAHGQFIVFDIQADGFLHHMVRNLMGTLYAIGRHELEPADFLNILAKKDRT
IAPPTASGDGLYFINAYYPEHFQTLLPNAPLTPIWLNLPD
>Mature_279_residues
TISMTENNEAKRPIYTLAIAIEFLGTHYHGWQRQREVLGVQEALETAISTVANEPVEVIAAGRTDSSVHAGNMIAHFVTH
AYRPSHNWLRGVNSLLPDDIALRWVQPMPADFHARFAAIARRYRYITLNQTQRPAILNHQVTHIHEPLDLKAMQLAAADI
VGTHDFSSYRAAACQSNQPVRHISHANLFAHGQFIVFDIQADGFLHHMVRNLMGTLYAIGRHELEPADFLNILAKKDRTI
APPTASGDGLYFINAYYPEHFQTLLPNAPLTPIWLNLPD

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=249, Percent_Identity=30.1204819277108, Blast_Score=82, Evalue=7e-16,
Organism=Escherichia coli, GI1788657, Length=269, Percent_Identity=48.3271375464684, Blast_Score=278, Evalue=3e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 31505; Mature: 31374

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTISMTENNEAKRPIYTLAIAIEFLGTHYHGWQRQREVLGVQEALETAISTVANEPVEVI
CEEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
AAGRTDSSVHAGNMIAHFVTHAYRPSHNWLRGVNSLLPDDIALRWVQPMPADFHARFAAI
EECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHEEECCCCCCHHHHHHHHH
ARRYRYITLNQTQRPAILNHQVTHIHEPLDLKAMQLAAADIVGTHDFSSYRAAACQSNQP
HHHHEEEEECCCCCCHHHCCCHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCC
VRHISHANLFAHGQFIVFDIQADGFLHHMVRNLMGTLYAIGRHELEPADFLNILAKKDRT
HHHHHHCCEEECCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
IAPPTASGDGLYFINAYYPEHFQTLLPNAPLTPIWLNLPD
CCCCCCCCCCEEEEEEECHHHHHHHCCCCCCCEEEEECCC
>Mature Secondary Structure 
TISMTENNEAKRPIYTLAIAIEFLGTHYHGWQRQREVLGVQEALETAISTVANEPVEVI
EEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
AAGRTDSSVHAGNMIAHFVTHAYRPSHNWLRGVNSLLPDDIALRWVQPMPADFHARFAAI
EECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHEEECCCCCCHHHHHHHHH
ARRYRYITLNQTQRPAILNHQVTHIHEPLDLKAMQLAAADIVGTHDFSSYRAAACQSNQP
HHHHEEEEECCCCCCHHHCCCHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCC
VRHISHANLFAHGQFIVFDIQADGFLHHMVRNLMGTLYAIGRHELEPADFLNILAKKDRT
HHHHHHCCEEECCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
IAPPTASGDGLYFINAYYPEHFQTLLPNAPLTPIWLNLPD
CCCCCCCCCCEEEEEEECHHHHHHHCCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA