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The map label for this gene is ansA [C]

Identifier: 93006391

GI number: 93006391

Start: 1923161

End: 1924246

Strand: Direct

Name: ansA [C]

Synonym: Pcryo_1567

Alternate gene names: 93006391

Gene position: 1923161-1924246 (Clockwise)

Preceding gene: 93006390

Following gene: 93006392

Centisome position: 62.85

GC content: 46.59

Gene sequence:

>1086_bases
ATGACAAATACAATCCCTGTACCTATCCAACTTATTTATGCTGGCGGCACTTTTGGGAGTTATGGTCGTCCATTAGCGCC
GCTATCAGCAGAGGTTTTTTTGCCAACTTTGCAGCAACTACTGGCAGAATCGGATAATGCGCTTAATCTATCGCCCATTG
CTTGGCTTCACAATACTCTGATTAAGGACAGCAGTCAGTTTACACCCAGTGATTTTGTGCATTTTTACCAGCTTTTATTG
TCGGCATATCAACAAGGCAGTAGACGTTTTGTGTTAATTACGGGCACGGATACACTCAGTTATTTGGGAGCGTTCTTAGC
AGAAGCCTTTGCAGGTAGCGATATTTGTATTGTCGTTACTGGTAGTATGCGTCCGCTCCTAGATAGTGAGGAACTGCACT
CTTATAAAGTAGATGCACAGAGTGATGCTTGGAACAACCTTACTGACTCGTTAAAGCTAGCAAGTGCAGGTGAATCTGGG
GTGAAGATTGCCTTCGGCGGCGAATCATGGCCAGCGCAAACGGTGCAAAAAATACATAGCCATGACTTTATGGCATTTAC
TGGTCACTCTAGAGCGGCTTATCCTGCCAATAGCTATGTCAAAAGCTTACCAGATACTCGTCGGCAGCACTGGCTTGATG
ACCAACAAGATATTAGTGCTGATATTGCAGTTCGTGCCAAGCACGCTTACGTTCATGCGTTGTACTGTTTGCCCAATGCA
ACCGACGCTTTAAACCAACAGCTGCGCGCCTTATTAACACAGCCGCCTTGTGGCATTATTTTATTAGGCTATGGCGCTGG
CAACGTCCCTTACTCAGTAAAGTTGGCAGAAGCCTTGCAACTGGCTTATGAAAAAGGTCATATGGTAGTGTGCGCTAGCC
AATCGCCGTTTGGTGGTGTGAGCGACACTTATGCTGCGGGCAGTTGGCAATACGATTATCAAGTGGTTGCAGGCGGTCGT
TTAACCATTCCGGCCATTTATGCACGCTTGCTATGGTTACTGCTACGCTACGATAGCCCAGCAAGGCGTCGACAACGCTG
GTTAAATAACGTCCAGCAAACGGCCACCAGTATCAAAAAACGTTAA

Upstream 100 bases:

>100_bases
ATAATGGTGCTTTTTCTATCTCTTCTCTAGCAATGATTTCTCTTTCAATAATTCTGCTCTATCGTCTATGTAATTTAAAA
CCAATACAGTGCACTCATCT

Downstream 100 bases:

>100_bases
ACTATCACTCACAAACTCTGTATAAGTTCCAAAATGACCATATCCATGACTGAAAACAACGAAGCAAAGCGCCCAATTTA
TACCTTAGCCATTGCTATTG

Product: putative L-asparaginase, type I

Products: L-aspartate; NH3

Alternate protein names: L-Asparaginase; L-Asparaginase Type I; L-Asparaginase I; Cytoplasmic Asparaginase I; 1-Alkyl-2-Acetylglycerophosphocholine Esterase; Cytoplasmic L-Asparaginase I; Asparaginase/Glutaminase

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MTNTIPVPIQLIYAGGTFGSYGRPLAPLSAEVFLPTLQQLLAESDNALNLSPIAWLHNTLIKDSSQFTPSDFVHFYQLLL
SAYQQGSRRFVLITGTDTLSYLGAFLAEAFAGSDICIVVTGSMRPLLDSEELHSYKVDAQSDAWNNLTDSLKLASAGESG
VKIAFGGESWPAQTVQKIHSHDFMAFTGHSRAAYPANSYVKSLPDTRRQHWLDDQQDISADIAVRAKHAYVHALYCLPNA
TDALNQQLRALLTQPPCGIILLGYGAGNVPYSVKLAEALQLAYEKGHMVVCASQSPFGGVSDTYAAGSWQYDYQVVAGGR
LTIPAIYARLLWLLLRYDSPARRRQRWLNNVQQTATSIKKR

Sequences:

>Translated_361_residues
MTNTIPVPIQLIYAGGTFGSYGRPLAPLSAEVFLPTLQQLLAESDNALNLSPIAWLHNTLIKDSSQFTPSDFVHFYQLLL
SAYQQGSRRFVLITGTDTLSYLGAFLAEAFAGSDICIVVTGSMRPLLDSEELHSYKVDAQSDAWNNLTDSLKLASAGESG
VKIAFGGESWPAQTVQKIHSHDFMAFTGHSRAAYPANSYVKSLPDTRRQHWLDDQQDISADIAVRAKHAYVHALYCLPNA
TDALNQQLRALLTQPPCGIILLGYGAGNVPYSVKLAEALQLAYEKGHMVVCASQSPFGGVSDTYAAGSWQYDYQVVAGGR
LTIPAIYARLLWLLLRYDSPARRRQRWLNNVQQTATSIKKR
>Mature_360_residues
TNTIPVPIQLIYAGGTFGSYGRPLAPLSAEVFLPTLQQLLAESDNALNLSPIAWLHNTLIKDSSQFTPSDFVHFYQLLLS
AYQQGSRRFVLITGTDTLSYLGAFLAEAFAGSDICIVVTGSMRPLLDSEELHSYKVDAQSDAWNNLTDSLKLASAGESGV
KIAFGGESWPAQTVQKIHSHDFMAFTGHSRAAYPANSYVKSLPDTRRQHWLDDQQDISADIAVRAKHAYVHALYCLPNAT
DALNQQLRALLTQPPCGIILLGYGAGNVPYSVKLAEALQLAYEKGHMVVCASQSPFGGVSDTYAAGSWQYDYQVVAGGRL
TIPAIYARLLWLLLRYDSPARRRQRWLNNVQQTATSIKKR

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.5.1.1

Molecular weight: Translated: 39702; Mature: 39571

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNTIPVPIQLIYAGGTFGSYGRPLAPLSAEVFLPTLQQLLAESDNALNLSPIAWLHNTL
CCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
IKDSSQFTPSDFVHFYQLLLSAYQQGSRRFVLITGTDTLSYLGAFLAEAFAGSDICIVVT
HCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHCCCCEEEEEE
GSMRPLLDSEELHSYKVDAQSDAWNNLTDSLKLASAGESGVKIAFGGESWPAQTVQKIHS
CCCCCCCCCHHHHEEECCCCCCCCCCHHHHHEEECCCCCCEEEEECCCCCCHHHHHHHHC
HDFMAFTGHSRAAYPANSYVKSLPDTRRQHWLDDQQDISADIAVRAKHAYVHALYCLPNA
CCCEEEECCCCCCCCHHHHHHHCCHHHHHHCCCCHHCCCCCEEEEEHHHEEEEEEECCCC
TDALNQQLRALLTQPPCGIILLGYGAGNVPYSVKLAEALQLAYEKGHMVVCASQSPFGGV
HHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEHHHHHHHHHHHCCCEEEEECCCCCCCC
SDTYAAGSWQYDYQVVAGGRLTIPAIYARLLWLLLRYDSPARRRQRWLNNVQQTATSIKK
CCCCCCCCEEEEEEEEECCEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
R
C
>Mature Secondary Structure 
TNTIPVPIQLIYAGGTFGSYGRPLAPLSAEVFLPTLQQLLAESDNALNLSPIAWLHNTL
CCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
IKDSSQFTPSDFVHFYQLLLSAYQQGSRRFVLITGTDTLSYLGAFLAEAFAGSDICIVVT
HCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHCCCCEEEEEE
GSMRPLLDSEELHSYKVDAQSDAWNNLTDSLKLASAGESGVKIAFGGESWPAQTVQKIHS
CCCCCCCCCHHHHEEECCCCCCCCCCHHHHHEEECCCCCCEEEEECCCCCCHHHHHHHHC
HDFMAFTGHSRAAYPANSYVKSLPDTRRQHWLDDQQDISADIAVRAKHAYVHALYCLPNA
CCCEEEECCCCCCCCHHHHHHHCCHHHHHHCCCCHHCCCCCEEEEEHHHEEEEEEECCCC
TDALNQQLRALLTQPPCGIILLGYGAGNVPYSVKLAEALQLAYEKGHMVVCASQSPFGGV
HHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEHHHHHHHHHHHCCCEEEEECCCCCCCC
SDTYAAGSWQYDYQVVAGGRLTIPAIYARLLWLLLRYDSPARRRQRWLNNVQQTATSIKK
CCCCCCCCEEEEEEEEECCEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-asparagine; H2O

Specific reaction: L-asparagine + H2O = L-aspartate + NH3

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: 5-Bromo-4-oxo-L-norvaline; Hg2+; NH3 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA