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The map label for this gene is queA

Identifier: 93006388

GI number: 93006388

Start: 1918190

End: 1919407

Strand: Direct

Name: queA

Synonym: Pcryo_1564

Alternate gene names: 93006388

Gene position: 1918190-1919407 (Clockwise)

Preceding gene: 93006387

Following gene: 93006389

Centisome position: 62.69

GC content: 43.68

Gene sequence:

>1218_bases
ATGACTGACTCTGAACCTCAAACCTTAAATAATAACTCTGAAATTACTGCCAGTCATAATGACGATATTTTAGAGTATCT
AAGTGTTGATGATTATGATTATGAGCTGCCAGACCAGCTGATTGCCCGTTATCCATTGGCACAGCGCTCCGCGTCAAAGC
TACTTTACTTGCGCGCTAACAATGCTAATAGTCAGGTTGAAGTTGAAGATAAGCTGTTTTCTGAGTTGCCAGAATTACTT
AATGCAGGCGACTTGATTGTTTTTAACGATACCAAGGTAATGAAAGCGCGCTTATTTGGTCAAAAAGATACCGGTGGAAA
GATTGAAGTATTAATAGAGCGTTTGGTCGCTCTCTCCGATTTAAATAGCGCGGATCTAGACACAATAACGTCAAACTCTG
ACATCATAGACATGACGGTTATTGCAGAAAAGCATATCGCTCTTTGTCATGTCAAAGCCAGTAAAGCGTTGAAGCTTGGA
CAAGGTCTTGTACTTGCTGATGGTCATATGACTGGTGTCATGATTGGACGCCAAGAGAACTTATTTATCTTGGCTTTTGA
TACGCCCATTCTGCCTGATTTAGAGCTATATGGTGAGCTGCCTATTCCACCCTATTTCGAACGCCATGCTGATGCGACCG
ACAATACTCGCTATCAGACTGTTTTTCATGATCCTGCTAAGCTTGCCAGCGTCGCTGCCCCGACAGCAAGCTTACATTTT
GACGATATTGTATTAGAAAAGCTGGCGGCAAAAGGTATTCAAACCGCTTTTGTTACACTGCATGTCGGCGCAGGTACTTT
TGCTCCGGTAAAAACAGAGAACCTGCTGAATCATACGATGCATAGCGAATATGCACACCTGCCTCAAGCCACTGCCGACC
TTATCAATCAGACCCATGCCAATGGCAAGCAAGTCATTGCTATCGGGACTACTGTCACACGTGTCCTTGAAACGGCTTAC
CAACAAACCGCAGTCGATAGACAGCCACTCTCTGGCTGGGCAGGTGATACAGAGATTTTTATATATCCCGGTTTTGAGTT
TGGCGTCGTGGATAAGCTACTGACCAACTTTCACTTGCCAAAGTCTACGTTGCTGATGCTAGTGTCCGCATTTGCGACAA
AAAAATCTATCGAAGAAGCTTATCAACATGCCATCGAATCCGAGTATCGGTTTTTTAGTTATGGTGATGCCATGCTGCTT
GATAAAAAAGTTGACTGA

Upstream 100 bases:

>100_bases
TAGAGGTAAATAAAAAAGTACCACTTAGTAAAACATTCTTTCATATCCTCATTTCTGCTACAATTGCTCTCAAAATTCTC
ATCTATCGAAAGACTTTCTT

Downstream 100 bases:

>100_bases
TGTGTGTAAGTATTAAACATGCAATTGAACTATTGGTCACAACAGCAGTGGAATAAACTGGTAAACTAAACAGTATTTTG
TTATAACAACACAGACAACT

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MTDSEPQTLNNNSEITASHNDDILEYLSVDDYDYELPDQLIARYPLAQRSASKLLYLRANNANSQVEVEDKLFSELPELL
NAGDLIVFNDTKVMKARLFGQKDTGGKIEVLIERLVALSDLNSADLDTITSNSDIIDMTVIAEKHIALCHVKASKALKLG
QGLVLADGHMTGVMIGRQENLFILAFDTPILPDLELYGELPIPPYFERHADATDNTRYQTVFHDPAKLASVAAPTASLHF
DDIVLEKLAAKGIQTAFVTLHVGAGTFAPVKTENLLNHTMHSEYAHLPQATADLINQTHANGKQVIAIGTTVTRVLETAY
QQTAVDRQPLSGWAGDTEIFIYPGFEFGVVDKLLTNFHLPKSTLLMLVSAFATKKSIEEAYQHAIESEYRFFSYGDAMLL
DKKVD

Sequences:

>Translated_405_residues
MTDSEPQTLNNNSEITASHNDDILEYLSVDDYDYELPDQLIARYPLAQRSASKLLYLRANNANSQVEVEDKLFSELPELL
NAGDLIVFNDTKVMKARLFGQKDTGGKIEVLIERLVALSDLNSADLDTITSNSDIIDMTVIAEKHIALCHVKASKALKLG
QGLVLADGHMTGVMIGRQENLFILAFDTPILPDLELYGELPIPPYFERHADATDNTRYQTVFHDPAKLASVAAPTASLHF
DDIVLEKLAAKGIQTAFVTLHVGAGTFAPVKTENLLNHTMHSEYAHLPQATADLINQTHANGKQVIAIGTTVTRVLETAY
QQTAVDRQPLSGWAGDTEIFIYPGFEFGVVDKLLTNFHLPKSTLLMLVSAFATKKSIEEAYQHAIESEYRFFSYGDAMLL
DKKVD
>Mature_404_residues
TDSEPQTLNNNSEITASHNDDILEYLSVDDYDYELPDQLIARYPLAQRSASKLLYLRANNANSQVEVEDKLFSELPELLN
AGDLIVFNDTKVMKARLFGQKDTGGKIEVLIERLVALSDLNSADLDTITSNSDIIDMTVIAEKHIALCHVKASKALKLGQ
GLVLADGHMTGVMIGRQENLFILAFDTPILPDLELYGELPIPPYFERHADATDNTRYQTVFHDPAKLASVAAPTASLHFD
DIVLEKLAAKGIQTAFVTLHVGAGTFAPVKTENLLNHTMHSEYAHLPQATADLINQTHANGKQVIAIGTTVTRVLETAYQ
QTAVDRQPLSGWAGDTEIFIYPGFEFGVVDKLLTNFHLPKSTLLMLVSAFATKKSIEEAYQHAIESEYRFFSYGDAMLLD
KKVD

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=377, Percent_Identity=45.6233421750663, Blast_Score=310, Evalue=1e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_PSYCK (Q1QAG2)

Other databases:

- EMBL:   CP000323
- RefSeq:   YP_580825.1
- ProteinModelPortal:   Q1QAG2
- SMR:   Q1QAG2
- STRING:   Q1QAG2
- GeneID:   4034416
- GenomeReviews:   CP000323_GR
- KEGG:   pcr:Pcryo_1564
- NMPDR:   fig|335284.3.peg.1407
- eggNOG:   COG0809
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- PhylomeDB:   Q1QAG2
- ProtClustDB:   PRK00147
- BioCyc:   PCRY335284:PCRYO_1564-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 44783; Mature: 44652

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDSEPQTLNNNSEITASHNDDILEYLSVDDYDYELPDQLIARYPLAQRSASKLLYLRAN
CCCCCCCCCCCCCEEEECCCCCHHHHHCCCCCCCCCCHHHHHHCCCHHCCCCEEEEEEEC
NANSQVEVEDKLFSELPELLNAGDLIVFNDTKVMKARLFGQKDTGGKIEVLIERLVALSD
CCCCEEEHHHHHHHHHHHHHCCCCEEEECCCCEEHHHHCCCCCCCCHHHHHHHHHHHHHC
LNSADLDTITSNSDIIDMTVIAEKHIALCHVKASKALKLGQGLVLADGHMTGVMIGRQEN
CCCCCCCEECCCCCEEEEEEEECCCEEEEEEEHHHHHHHCCCEEEECCCEEEEEEECCCC
LFILAFDTPILPDLELYGELPIPPYFERHADATDNTRYQTVFHDPAKLASVAAPTASLHF
EEEEEECCCCCCCHHHCCCCCCCCHHHHCCCCCCCCEEEEEECCHHHHHHHCCCCCCCCH
DDIVLEKLAAKGIQTAFVTLHVGAGTFAPVKTENLLNHTMHSEYAHLPQATADLINQTHA
HHHHHHHHHHCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCC
NGKQVIAIGTTVTRVLETAYQQTAVDRQPLSGWAGDTEIFIYPGFEFGVVDKLLTNFHLP
CCCEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCC
KSTLLMLVSAFATKKSIEEAYQHAIESEYRFFSYGDAMLLDKKVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCEEEEECCCC
>Mature Secondary Structure 
TDSEPQTLNNNSEITASHNDDILEYLSVDDYDYELPDQLIARYPLAQRSASKLLYLRAN
CCCCCCCCCCCCEEEECCCCCHHHHHCCCCCCCCCCHHHHHHCCCHHCCCCEEEEEEEC
NANSQVEVEDKLFSELPELLNAGDLIVFNDTKVMKARLFGQKDTGGKIEVLIERLVALSD
CCCCEEEHHHHHHHHHHHHHCCCCEEEECCCCEEHHHHCCCCCCCCHHHHHHHHHHHHHC
LNSADLDTITSNSDIIDMTVIAEKHIALCHVKASKALKLGQGLVLADGHMTGVMIGRQEN
CCCCCCCEECCCCCEEEEEEEECCCEEEEEEEHHHHHHHCCCEEEECCCEEEEEEECCCC
LFILAFDTPILPDLELYGELPIPPYFERHADATDNTRYQTVFHDPAKLASVAAPTASLHF
EEEEEECCCCCCCHHHCCCCCCCCHHHHCCCCCCCCEEEEEECCHHHHHHHCCCCCCCCH
DDIVLEKLAAKGIQTAFVTLHVGAGTFAPVKTENLLNHTMHSEYAHLPQATADLINQTHA
HHHHHHHHHHCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCC
NGKQVIAIGTTVTRVLETAYQQTAVDRQPLSGWAGDTEIFIYPGFEFGVVDKLLTNFHLP
CCCEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCC
KSTLLMLVSAFATKKSIEEAYQHAIESEYRFFSYGDAMLLDKKVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA