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The map label for this gene is mltB [H]

Identifier: 93005492

GI number: 93005492

Start: 784478

End: 785779

Strand: Direct

Name: mltB [H]

Synonym: Pcryo_0662

Alternate gene names: 93005492

Gene position: 784478-785779 (Clockwise)

Preceding gene: 93005491

Following gene: 93005495

Centisome position: 25.64

GC content: 47.93

Gene sequence:

>1302_bases
ATGCGATTAAATAAACACTACCTTGCCTTTTTCCCTGCCTTAGTAATGGTTGGTTGTACCAGTCAGCAGGCGATGCAGCA
ACCTAATAAGCCAGTTCGTCAAGGTAATGTGCAAATTACCCAGACTCAAACCATTCAAGTGAAGCCGACTCCAGCACCGG
TCATTAAACCAACACCCGCAAAGCCAAGCTACAATAGTTTTTCTGATTGGAAGTCTGATTTTTCTATGCGTGCCATTTCA
TCAGGTCATGATGCTGCTACGGTTCGTCGTCTGCTCGATTCTGCGTATTTAAATCAACAAGTCATCTCGCTTGATTCAGG
ACAGCCAGAGTTTTCAAAAATGCCATGGGAATATGTCGATTCTGCGGTATCAGATGGTCGCGTCAGTACTGGCAAGCGTA
AGTTCGCTGAGCAACGCACGTATTTATCGCAGTTACAGTCGCAATATGGCGTCAATGCAGAAATCATTGCAGCGATTTGG
GGCATGGAGTCGTCATACGGCGTAGTAACAGGTAATAGTAGTATTCCAAGCTCGCTAGCTAGCCTTGCTTATGATGGGCG
CCGTCAAGAATTTGCCGAAACTCAGCTATTATCACTAGCGACACTATTACAGCGCGGCGATGTGTCTTGGTCACAGCTCG
ACGGCTCTTGGGCAGGCGGTATGGGGCAGACGCAGTTTATCCCTGATACCTGGCTTAAACATGGCGTTGACGGTGATGGT
AATGGACATCGCAATCCATGGTCGACGGGCGACGCCTTGGCATCTACTGCTAATTATCTGAGCAATTCAGGTTGGGTACG
TGGTTTGGCACCATTTTATGAAGCAACCATTCCGTCATCATTTGATTACACACTGGTTGGTACTAAGCAGCCTGCTGCTA
GATGGGCGTCAATGGGCGTCGATACCATAGCCGATGTGTCCTTGGATGGTAACACTCAAATGGAGTTGTGGCTGCCAGCG
GGTAAAGACGGTCCAGTACTGCTACTCAGTCCAAATTTTGATGTCATAAAAGTCTATAACAATTCATCAAGCTATGCCCT
TGGTGTTAGCTTACTGGGTAAAGCGCTGATTGGACAAGGCGGTTTGCAAAAGTCATGGCCGCGTTATGAGCGTCCGTTAT
CAACGTCGCAAGTACGCAATTTACAACAGCGTTTGACAAGCTCAGGCTACGATACAAAAGGCTCTGATGGTATCGTAGGT
ACCAATACTCGCAATGCATTCCAGCGCTGGCAAGCAGCCAACGGTCAAACGCCAGATGGTTTTATTACTCAGCGTAGCGC
GTCTTCATTGGCTGGGTGGTGA

Upstream 100 bases:

>100_bases
CCACGAGCGGTACTAGGGCGTTAAATTTATTATTGTTTGGCAATTAAATGGAATTATCTAAGCCACATTGCACCTTACTT
ACGGATACTTGAGACCTCTT

Downstream 100 bases:

>100_bases
GATAGGTTGAAATCACTATTTTTGGATAATCGTTGTTTTCAAAGGTTGTTTAAAAATAACGATGTTACTAAATAAAAAAA
ACTCCGTTTTATAACGGAGT

Product: lytic murein transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MRLNKHYLAFFPALVMVGCTSQQAMQQPNKPVRQGNVQITQTQTIQVKPTPAPVIKPTPAKPSYNSFSDWKSDFSMRAIS
SGHDAATVRRLLDSAYLNQQVISLDSGQPEFSKMPWEYVDSAVSDGRVSTGKRKFAEQRTYLSQLQSQYGVNAEIIAAIW
GMESSYGVVTGNSSIPSSLASLAYDGRRQEFAETQLLSLATLLQRGDVSWSQLDGSWAGGMGQTQFIPDTWLKHGVDGDG
NGHRNPWSTGDALASTANYLSNSGWVRGLAPFYEATIPSSFDYTLVGTKQPAARWASMGVDTIADVSLDGNTQMELWLPA
GKDGPVLLLSPNFDVIKVYNNSSSYALGVSLLGKALIGQGGLQKSWPRYERPLSTSQVRNLQQRLTSSGYDTKGSDGIVG
TNTRNAFQRWQAANGQTPDGFITQRSASSLAGW

Sequences:

>Translated_433_residues
MRLNKHYLAFFPALVMVGCTSQQAMQQPNKPVRQGNVQITQTQTIQVKPTPAPVIKPTPAKPSYNSFSDWKSDFSMRAIS
SGHDAATVRRLLDSAYLNQQVISLDSGQPEFSKMPWEYVDSAVSDGRVSTGKRKFAEQRTYLSQLQSQYGVNAEIIAAIW
GMESSYGVVTGNSSIPSSLASLAYDGRRQEFAETQLLSLATLLQRGDVSWSQLDGSWAGGMGQTQFIPDTWLKHGVDGDG
NGHRNPWSTGDALASTANYLSNSGWVRGLAPFYEATIPSSFDYTLVGTKQPAARWASMGVDTIADVSLDGNTQMELWLPA
GKDGPVLLLSPNFDVIKVYNNSSSYALGVSLLGKALIGQGGLQKSWPRYERPLSTSQVRNLQQRLTSSGYDTKGSDGIVG
TNTRNAFQRWQAANGQTPDGFITQRSASSLAGW
>Mature_433_residues
MRLNKHYLAFFPALVMVGCTSQQAMQQPNKPVRQGNVQITQTQTIQVKPTPAPVIKPTPAKPSYNSFSDWKSDFSMRAIS
SGHDAATVRRLLDSAYLNQQVISLDSGQPEFSKMPWEYVDSAVSDGRVSTGKRKFAEQRTYLSQLQSQYGVNAEIIAAIW
GMESSYGVVTGNSSIPSSLASLAYDGRRQEFAETQLLSLATLLQRGDVSWSQLDGSWAGGMGQTQFIPDTWLKHGVDGDG
NGHRNPWSTGDALASTANYLSNSGWVRGLAPFYEATIPSSFDYTLVGTKQPAARWASMGVDTIADVSLDGNTQMELWLPA
GKDGPVLLLSPNFDVIKVYNNSSSYALGVSLLGKALIGQGGLQKSWPRYERPLSTSQVRNLQQRLTSSGYDTKGSDGIVG
TNTRNAFQRWQAANGQTPDGFITQRSASSLAGW

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=292, Percent_Identity=30.1369863013699, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 47132; Mature: 47132

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLNKHYLAFFPALVMVGCTSQQAMQQPNKPVRQGNVQITQTQTIQVKPTPAPVIKPTPA
CCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCHHCCCEEEEEEEEEEECCCCCCCCCCCCC
KPSYNSFSDWKSDFSMRAISSGHDAATVRRLLDSAYLNQQVISLDSGQPEFSKMPWEYVD
CCCCCCHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHCCCEEECCCCCCHHHCCCHHHHH
SAVSDGRVSTGKRKFAEQRTYLSQLQSQYGVNAEIIAAIWGMESSYGVVTGNSSIPSSLA
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEHEECCCCCCCEEEECCCCCHHHHH
SLAYDGRRQEFAETQLLSLATLLQRGDVSWSQLDGSWAGGMGQTQFIPDTWLKHGVDGDG
HHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCC
NGHRNPWSTGDALASTANYLSNSGWVRGLAPFYEATIPSSFDYTLVGTKQPAARWASMGV
CCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCEEEEECCCHHHHHHHCCC
DTIADVSLDGNTQMELWLPAGKDGPVLLLSPNFDVIKVYNNSSSYALGVSLLGKALIGQG
CHHEEEECCCCCEEEEEEECCCCCCEEEECCCCCEEEEEECCCCEEHHHHHHHHHHHCCC
GLQKSWPRYERPLSTSQVRNLQQRLTSSGYDTKGSDGIVGTNTRNAFQRWQAANGQTPDG
CCCCCCCHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCEEECCCHHHHHHHHHCCCCCCCC
FITQRSASSLAGW
CEECCCCHHCCCC
>Mature Secondary Structure
MRLNKHYLAFFPALVMVGCTSQQAMQQPNKPVRQGNVQITQTQTIQVKPTPAPVIKPTPA
CCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCHHCCCEEEEEEEEEEECCCCCCCCCCCCC
KPSYNSFSDWKSDFSMRAISSGHDAATVRRLLDSAYLNQQVISLDSGQPEFSKMPWEYVD
CCCCCCHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHCCCEEECCCCCCHHHCCCHHHHH
SAVSDGRVSTGKRKFAEQRTYLSQLQSQYGVNAEIIAAIWGMESSYGVVTGNSSIPSSLA
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEHEECCCCCCCEEEECCCCCHHHHH
SLAYDGRRQEFAETQLLSLATLLQRGDVSWSQLDGSWAGGMGQTQFIPDTWLKHGVDGDG
HHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCC
NGHRNPWSTGDALASTANYLSNSGWVRGLAPFYEATIPSSFDYTLVGTKQPAARWASMGV
CCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCEEEEECCCHHHHHHHCCC
DTIADVSLDGNTQMELWLPAGKDGPVLLLSPNFDVIKVYNNSSSYALGVSLLGKALIGQG
CHHEEEECCCCCEEEEEEECCCCCCEEEECCCCCEEEEEECCCCEEHHHHHHHHHHHCCC
GLQKSWPRYERPLSTSQVRNLQQRLTSSGYDTKGSDGIVGTNTRNAFQRWQAANGQTPDG
CCCCCCCHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCEEECCCHHHHHHHHHCCCCCCCC
FITQRSASSLAGW
CEECCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]