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The map label for this gene is spsK [H]

Identifier: 93005457

GI number: 93005457

Start: 745780

End: 746643

Strand: Direct

Name: spsK [H]

Synonym: Pcryo_0627

Alternate gene names: 93005457

Gene position: 745780-746643 (Clockwise)

Preceding gene: 93005456

Following gene: 93005458

Centisome position: 24.37

GC content: 41.78

Gene sequence:

>864_bases
ATGTTTAGAGTCCTAGTGACCGGTGCTAAAGGGCAGGTTGGCAGCGAGTTGGTTAATTTGGTGCCTGCAGGCTTTACTGT
GATTGGGCTGAGTTCTGCTCAATTGGATATTACTGATCAAGATCAAGTAAATGTAGCAGTTGCTCTGCACCAGCCTAATC
TAATCATTAACGCCGCAGCTTATACCGCAGTTGATAAAGCTGAGAGTGATTCAGATAACGCATATGCTATTAATGAAAAG
GCTGTAGCTTGGTTAGCTCAAGCTGCTGCGCATGCCGATATTCCATTGTTTCATATTTCAACCGATTATGTCTTTGATGG
CGAGTCTACAACGCCATACAAAGAAACTGACTCGATTAACCCGCAAAGTGTTTACGGTGCGAGCAAGCTTGCTGGTGAGC
AAATGTTAGCCGCGACTCATAATAAGCATATTATTTTGCGTACCAGTTGGGTGTTTAGTGCGCGAGGCAATAACTTTGTA
AAAACTATGCTGCGTTTAGCTAAAGAGCGGGATGAATTATCAATAGTTGGTGATCAGCACGGCTGCCCTACTAGTGCGAC
TAGTATTGCAGATATCTTGTGGAAACTAGCTGAGCTATATCAACAAACTGATAGTCTGCCTTGGGGTATTTATAATTTTA
GTAATTCCTCCGCTTGCAGTTGGTATGAATTTGCTGTTGAAATTTTTAAGCAAGCAAGAGAGAGTGGTTTTATACCGCAA
ACACCTACTATCAAAAAAATCACTACTGCTGACTACCCTACGCCTGCTGTAAGGCCAGCTTTTTCAGTCTTGGACTGTAG
TAAACTAGAAAAATTATTAGGACAAAAGATTCCAGAATGGAAGTCAGAGTTGAGTACTATATGA

Upstream 100 bases:

>100_bases
GGCGGTATAGCGTGGAATTGCCCCTTGCTCAATATTGATTGGCCGATTAGTGATGTAAAGCTGTCAGATAAAGATAAAGC
CTATGCGGGGTTGGAAGCTC

Downstream 100 bases:

>100_bases
TAGATATTGTAATTGTAAATTGGAATAGTGGCATTCAGTTAAAGCAATGTATTGATTCTATTCTTGCCCATAGTATAAAT
AAGATTTCACGTATTATTAT

Product: dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MFRVLVTGAKGQVGSELVNLVPAGFTVIGLSSAQLDITDQDQVNVAVALHQPNLIINAAAYTAVDKAESDSDNAYAINEK
AVAWLAQAAAHADIPLFHISTDYVFDGESTTPYKETDSINPQSVYGASKLAGEQMLAATHNKHIILRTSWVFSARGNNFV
KTMLRLAKERDELSIVGDQHGCPTSATSIADILWKLAELYQQTDSLPWGIYNFSNSSACSWYEFAVEIFKQARESGFIPQ
TPTIKKITTADYPTPAVRPAFSVLDCSKLEKLLGQKIPEWKSELSTI

Sequences:

>Translated_287_residues
MFRVLVTGAKGQVGSELVNLVPAGFTVIGLSSAQLDITDQDQVNVAVALHQPNLIINAAAYTAVDKAESDSDNAYAINEK
AVAWLAQAAAHADIPLFHISTDYVFDGESTTPYKETDSINPQSVYGASKLAGEQMLAATHNKHIILRTSWVFSARGNNFV
KTMLRLAKERDELSIVGDQHGCPTSATSIADILWKLAELYQQTDSLPWGIYNFSNSSACSWYEFAVEIFKQARESGFIPQ
TPTIKKITTADYPTPAVRPAFSVLDCSKLEKLLGQKIPEWKSELSTI
>Mature_287_residues
MFRVLVTGAKGQVGSELVNLVPAGFTVIGLSSAQLDITDQDQVNVAVALHQPNLIINAAAYTAVDKAESDSDNAYAINEK
AVAWLAQAAAHADIPLFHISTDYVFDGESTTPYKETDSINPQSVYGASKLAGEQMLAATHNKHIILRTSWVFSARGNNFV
KTMLRLAKERDELSIVGDQHGCPTSATSIADILWKLAELYQQTDSLPWGIYNFSNSSACSWYEFAVEIFKQARESGFIPQ
TPTIKKITTADYPTPAVRPAFSVLDCSKLEKLLGQKIPEWKSELSTI

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI11034825, Length=290, Percent_Identity=30, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI33519455, Length=290, Percent_Identity=30, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI1788352, Length=284, Percent_Identity=39.4366197183099, Blast_Score=201, Evalue=6e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 31374; Mature: 31374

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRVLVTGAKGQVGSELVNLVPAGFTVIGLSSAQLDITDQDQVNVAVALHQPNLIINAAA
CEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCEEECCCCCCEEEEEEEECCCEEEEEHH
YTAVDKAESDSDNAYAINEKAVAWLAQAAAHADIPLFHISTDYVFDGESTTPYKETDSIN
HHHHHCCCCCCCCEEEECHHHHHHHHHHHHHCCCEEEEEEECEEECCCCCCCCCCCCCCC
PQSVYGASKLAGEQMLAATHNKHIILRTSWVFSARGNNFVKTMLRLAKERDELSIVGDQH
CHHHCCHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCHHHHHHHHHHHCCCCEEEECCCC
GCPTSATSIADILWKLAELYQQTDSLPWGIYNFSNSSACSWYEFAVEIFKQARESGFIPQ
CCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCC
TPTIKKITTADYPTPAVRPAFSVLDCSKLEKLLGQKIPEWKSELSTI
CCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCC
>Mature Secondary Structure
MFRVLVTGAKGQVGSELVNLVPAGFTVIGLSSAQLDITDQDQVNVAVALHQPNLIINAAA
CEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCEEECCCCCCEEEEEEEECCCEEEEEHH
YTAVDKAESDSDNAYAINEKAVAWLAQAAAHADIPLFHISTDYVFDGESTTPYKETDSIN
HHHHHCCCCCCCCEEEECHHHHHHHHHHHHHCCCEEEEEEECEEECCCCCCCCCCCCCCC
PQSVYGASKLAGEQMLAATHNKHIILRTSWVFSARGNNFVKTMLRLAKERDELSIVGDQH
CHHHCCHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCHHHHHHHHHHHCCCCEEEECCCC
GCPTSATSIADILWKLAELYQQTDSLPWGIYNFSNSSACSWYEFAVEIFKQARESGFIPQ
CCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCC
TPTIKKITTADYPTPAVRPAFSVLDCSKLEKLLGQKIPEWKSELSTI
CCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]