Click here to switch to the map view.

The map label for this gene is capD [H]

Identifier: 93005442

GI number: 93005442

Start: 728411

End: 730363

Strand: Direct

Name: capD [H]

Synonym: Pcryo_0612

Alternate gene names: 93005442

Gene position: 728411-730363 (Clockwise)

Preceding gene: 93005441

Following gene: 93005443

Centisome position: 23.81

GC content: 40.96

Gene sequence:

>1953_bases
ATGCCAATAGACACATCAATAATCTCTAAAAAAGATAAGACCTCTCCTATAGAGTCTAATGACTTTGCTGAGCGTACTGC
TAAGATATTGCTGAATCTACCTCGAGGCATCAAGCGTAGTATTCTGATGATGGCTGATTTTATCATGTCAGTGGTCTGTC
TATTCTTTGCCGTAGCAGCGCGCTACGGGTATATAGACAATCACGTGAGTTTATTATTATTAAGCGTCAGTGCATTGATT
CCAGTATTATGCTTATATGTAATTGGATTTTATAATGGCGTGGCACGAGGGTTCTTTGATGCGATGATGGGCAGTGTATT
ACAACTTTTCTTTGTACTCATCATCGTTGCCCAAACTGTTTTATATTTTAAATTACTACCAGGTATTCCTCGTGCAGTAC
CTATCTTGTATTTATTTCTATTTTTTATATGGCTGTGGAATAGTCGCTTAACGATTAGAGAGTTGCTAGCAAGGTGGCAA
GGTCAAAGACCACAGTATAAGTATGATGATTATGATAACGTTGTCATCTATGGTGCAGGAGAGGCCGGCAAAGAGCTCTT
AGAAGGTTTAAGAAACTCTCATAAGTATAATGTCGTTGCTTATGTCGATGACGATCCGCAATTGACAGGCGCTTATTTAC
TCGGTAAACAAATTCACGCGGCTTCTGACCTTATTGGGCTCGTAGGTAAACTAGATATTGCCCAAGTTTTTTTAGCCATT
CCTTCTATCAGTCGGTCACGCAAACGCCAAATCATTGATAAACTAGAAGGTATCTCTATTAAAATCAAGGAATTGCCTAG
CTTAGAAGAAATCGCTGAAGAAAAAGTGACTGTTAGTAGTATGCGTAGAGTGGATATATTAGATGTATTAGATCGGCAGA
CAGTTGAACCTGACGTTAATCTTTTACAGCAAAATATTACGGGTAAAAGTGTACTGGTGACAGGCGCTGGTGGATCTATC
GGTAGCGAATTGTGTCGTCAAATTATTAAGAATAAGCCTAAATGCTTGGTACTATATGAATTATCTGAGTACGCGCTTTA
TACGATTGATCAAGAGTTGACGGCGAAAAAAGCTAAAAATAAAGACGACCATGCTATAGAGATCGTTGCCATTATTGGCA
ATGTGACCAACGAAGACAACTTGCTGCGTATATTGAACCTCTATCAAATACAGACGGTCTATCATGCGGCTGCTTATAAA
CACGTCCCCATCGTAGAGCATAATCCGTTTGAAGGTGTGATTAATAATAGTAAAGGCACCTATCACTGTGCGCGTGCCGC
GATACAAGCGAAGGTTGACACCTTTGTACTGATCTCCACCGACAAAGCAGTCAGACCGACTAATATAATGGGAGCATCTA
AGCGTTTAGCAGAGTTAGTCTGTCAAGGGCTTAGTATGACGACGGCTCATACTTGTATCAGTATGGTGCGCTTCGGCAAT
GTGCTTGGGTCCTCAGGCTCGGTAGTCCCTGTGTTTACGAAGCAGATAGAACAGGGGCGACCTATTACGGTTACTCATCC
TGATGTTACACGTTATTTTATGACGATTCCTGAAGCCGCCAATCTAGTGATTCAAGCAGGGGCGATGGCAAAAGGCGGTG
AAGTATTCGTTTTGGATATGGGTGAACCAGTCAAAATCGTCGATTTAGCACGTCGTATGATTCATTTAAGTGGACACAGA
GCAAAGACTAATGATAATCCTAACGGCGATATAGAGATAATTTTTACTGGTTTGAGACCAGGTGAAAAGCTATACGAAGA
GTTGATCATCGGTGAGGATAATATCGAAAATACTCATCATCCACTGATCATGCAAGCGATAGAGCATAGCTTTCCATTAG
ATGAGCTTGAATCGCTACTGGCTGAGCTCACTGAAAAGCAAAAAGAGTTTGACGTTGCTTGGTTAAAGCAGCAGTTTAAG
CAGTTTGTAGATGGTTATAGACAAGGTTCTTAG

Upstream 100 bases:

>100_bases
TTATCATTATAGTTATGCTTACGGTAATAATAACTAATATGTATTTGAGACAGACTGTATTACCTTAATTATTAGTATTT
AAACGAAATAGTTGCTTGAT

Downstream 100 bases:

>100_bases
CTTGTATAGAAAAATGTAATGACTGAAATACTCACATTATTACCCATCAACTAGTAAGATTATTTAAAGAGAATTTATGA
TAGATATCAATAGTATCAAA

Product: polysaccharide biosynthesis protein CapD

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 650; Mature: 649

Protein sequence:

>650_residues
MPIDTSIISKKDKTSPIESNDFAERTAKILLNLPRGIKRSILMMADFIMSVVCLFFAVAARYGYIDNHVSLLLLSVSALI
PVLCLYVIGFYNGVARGFFDAMMGSVLQLFFVLIIVAQTVLYFKLLPGIPRAVPILYLFLFFIWLWNSRLTIRELLARWQ
GQRPQYKYDDYDNVVIYGAGEAGKELLEGLRNSHKYNVVAYVDDDPQLTGAYLLGKQIHAASDLIGLVGKLDIAQVFLAI
PSISRSRKRQIIDKLEGISIKIKELPSLEEIAEEKVTVSSMRRVDILDVLDRQTVEPDVNLLQQNITGKSVLVTGAGGSI
GSELCRQIIKNKPKCLVLYELSEYALYTIDQELTAKKAKNKDDHAIEIVAIIGNVTNEDNLLRILNLYQIQTVYHAAAYK
HVPIVEHNPFEGVINNSKGTYHCARAAIQAKVDTFVLISTDKAVRPTNIMGASKRLAELVCQGLSMTTAHTCISMVRFGN
VLGSSGSVVPVFTKQIEQGRPITVTHPDVTRYFMTIPEAANLVIQAGAMAKGGEVFVLDMGEPVKIVDLARRMIHLSGHR
AKTNDNPNGDIEIIFTGLRPGEKLYEELIIGEDNIENTHHPLIMQAIEHSFPLDELESLLAELTEKQKEFDVAWLKQQFK
QFVDGYRQGS

Sequences:

>Translated_650_residues
MPIDTSIISKKDKTSPIESNDFAERTAKILLNLPRGIKRSILMMADFIMSVVCLFFAVAARYGYIDNHVSLLLLSVSALI
PVLCLYVIGFYNGVARGFFDAMMGSVLQLFFVLIIVAQTVLYFKLLPGIPRAVPILYLFLFFIWLWNSRLTIRELLARWQ
GQRPQYKYDDYDNVVIYGAGEAGKELLEGLRNSHKYNVVAYVDDDPQLTGAYLLGKQIHAASDLIGLVGKLDIAQVFLAI
PSISRSRKRQIIDKLEGISIKIKELPSLEEIAEEKVTVSSMRRVDILDVLDRQTVEPDVNLLQQNITGKSVLVTGAGGSI
GSELCRQIIKNKPKCLVLYELSEYALYTIDQELTAKKAKNKDDHAIEIVAIIGNVTNEDNLLRILNLYQIQTVYHAAAYK
HVPIVEHNPFEGVINNSKGTYHCARAAIQAKVDTFVLISTDKAVRPTNIMGASKRLAELVCQGLSMTTAHTCISMVRFGN
VLGSSGSVVPVFTKQIEQGRPITVTHPDVTRYFMTIPEAANLVIQAGAMAKGGEVFVLDMGEPVKIVDLARRMIHLSGHR
AKTNDNPNGDIEIIFTGLRPGEKLYEELIIGEDNIENTHHPLIMQAIEHSFPLDELESLLAELTEKQKEFDVAWLKQQFK
QFVDGYRQGS
>Mature_649_residues
PIDTSIISKKDKTSPIESNDFAERTAKILLNLPRGIKRSILMMADFIMSVVCLFFAVAARYGYIDNHVSLLLLSVSALIP
VLCLYVIGFYNGVARGFFDAMMGSVLQLFFVLIIVAQTVLYFKLLPGIPRAVPILYLFLFFIWLWNSRLTIRELLARWQG
QRPQYKYDDYDNVVIYGAGEAGKELLEGLRNSHKYNVVAYVDDDPQLTGAYLLGKQIHAASDLIGLVGKLDIAQVFLAIP
SISRSRKRQIIDKLEGISIKIKELPSLEEIAEEKVTVSSMRRVDILDVLDRQTVEPDVNLLQQNITGKSVLVTGAGGSIG
SELCRQIIKNKPKCLVLYELSEYALYTIDQELTAKKAKNKDDHAIEIVAIIGNVTNEDNLLRILNLYQIQTVYHAAAYKH
VPIVEHNPFEGVINNSKGTYHCARAAIQAKVDTFVLISTDKAVRPTNIMGASKRLAELVCQGLSMTTAHTCISMVRFGNV
LGSSGSVVPVFTKQIEQGRPITVTHPDVTRYFMTIPEAANLVIQAGAMAKGGEVFVLDMGEPVKIVDLARRMIHLSGHRA
KTNDNPNGDIEIIFTGLRPGEKLYEELIIGEDNIENTHHPLIMQAIEHSFPLDELESLLAELTEKQKEFDVAWLKQQFKQ
FVDGYRQGS

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: COG1086

COG function: function code MG; Predicted nucleoside-diphosphate sugar epimerases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR003869 [H]

Pfam domain/function: PF02719 Polysacc_synt_2 [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 72567; Mature: 72436

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIDTSIISKKDKTSPIESNDFAERTAKILLNLPRGIKRSILMMADFIMSVVCLFFAVAA
CCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RYGYIDNHVSLLLLSVSALIPVLCLYVIGFYNGVARGFFDAMMGSVLQLFFVLIIVAQTV
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYFKLLPGIPRAVPILYLFLFFIWLWNSRLTIRELLARWQGQRPQYKYDDYDNVVIYGAG
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECC
EAGKELLEGLRNSHKYNVVAYVDDDPQLTGAYLLGKQIHAASDLIGLVGKLDIAQVFLAI
HHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PSISRSRKRQIIDKLEGISIKIKELPSLEEIAEEKVTVSSMRRVDILDVLDRQTVEPDVN
CCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
LLQQNITGKSVLVTGAGGSIGSELCRQIIKNKPKCLVLYELSEYALYTIDQELTAKKAKN
HHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCEEEEECHHHHHHHCCC
KDDHAIEIVAIIGNVTNEDNLLRILNLYQIQTVYHAAAYKHVPIVEHNPFEGVINNSKGT
CCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
YHCARAAIQAKVDTFVLISTDKAVRPTNIMGASKRLAELVCQGLSMTTAHTCISMVRFGN
HHHHHHHHHHHCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
VLGSSGSVVPVFTKQIEQGRPITVTHPDVTRYFMTIPEAANLVIQAGAMAKGGEVFVLDM
HHCCCCCEEHHHHHHHHCCCCEEEECCHHHHHHHCCCHHHHHEEEECCCCCCCEEEEEEC
GEPVKIVDLARRMIHLSGHRAKTNDNPNGDIEIIFTGLRPGEKLYEELIIGEDNIENTHH
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCC
PLIMQAIEHSFPLDELESLLAELTEKQKEFDVAWLKQQFKQFVDGYRQGS
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PIDTSIISKKDKTSPIESNDFAERTAKILLNLPRGIKRSILMMADFIMSVVCLFFAVAA
CCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RYGYIDNHVSLLLLSVSALIPVLCLYVIGFYNGVARGFFDAMMGSVLQLFFVLIIVAQTV
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYFKLLPGIPRAVPILYLFLFFIWLWNSRLTIRELLARWQGQRPQYKYDDYDNVVIYGAG
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECC
EAGKELLEGLRNSHKYNVVAYVDDDPQLTGAYLLGKQIHAASDLIGLVGKLDIAQVFLAI
HHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PSISRSRKRQIIDKLEGISIKIKELPSLEEIAEEKVTVSSMRRVDILDVLDRQTVEPDVN
CCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
LLQQNITGKSVLVTGAGGSIGSELCRQIIKNKPKCLVLYELSEYALYTIDQELTAKKAKN
HHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCEEEEECHHHHHHHCCC
KDDHAIEIVAIIGNVTNEDNLLRILNLYQIQTVYHAAAYKHVPIVEHNPFEGVINNSKGT
CCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
YHCARAAIQAKVDTFVLISTDKAVRPTNIMGASKRLAELVCQGLSMTTAHTCISMVRFGN
HHHHHHHHHHHCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
VLGSSGSVVPVFTKQIEQGRPITVTHPDVTRYFMTIPEAANLVIQAGAMAKGGEVFVLDM
HHCCCCCEEHHHHHHHHCCCCEEEECCHHHHHHHCCCHHHHHEEEECCCCCCCEEEEEEC
GEPVKIVDLARRMIHLSGHRAKTNDNPNGDIEIIFTGLRPGEKLYEELIIGEDNIENTHH
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCC
PLIMQAIEHSFPLDELESLLAELTEKQKEFDVAWLKQQFKQFVDGYRQGS
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]