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The map label for this gene is nuoF [H]

Identifier: 93005407

GI number: 93005407

Start: 687733

End: 689157

Strand: Direct

Name: nuoF [H]

Synonym: Pcryo_0577

Alternate gene names: 93005407

Gene position: 687733-689157 (Clockwise)

Preceding gene: 93005406

Following gene: 93005408

Centisome position: 22.48

GC content: 46.53

Gene sequence:

>1425_bases
ATGAGATTAACTGTTTCAGAGCAGATTGCTCTTCGTAATCAAGGCAAAGCGCCAAGCCAACTTAATGAACAGCGCATTCC
TATCTATGGCGATAGAGCGACTGCTACCAGTGAAACCAAACCACTGACCTGGCGCTTAGCGCATCATGATGCCGTGCTTG
ATTTGGCTACTTATGAATCATTACGTGGTTTTGAAGCGTTAAAGATAGCGTTAAACCAATCGCCAGATGATACGTTAAAT
ACTATTAAAGAAGCGGTGGTAAAAGGTCGCGGCGGTGCAGGTTTTCCAGCAGGTATTAAATGGTCGCTAATGGCACCGCC
AGATGGCGGTCCACGCTATCTAGTCTGTAATGCTGATGAGATGGAGCCGGGTACGTTTAAAGACCGTTTGCTCATGGAGC
GTTTGCCGCTTCAGCTTATTGAAGGCATGCTTATTTCTGCCTATGCGATTGGTGCAACAGCCGGTTATATCTTTATCCGT
GGTGAATATATCTTAGCTGCGGAGCGTTTAACGGCAGCGATCGAAGAATTAAAAGCCAATAATCTAGTCGGCGATAATAT
TTTAGGCTCAGATTTTAGTTTTGATTTACATGTGCATACGGGTGCTGGTCGTTATATTTGCGGTGAAGAAACGGCATTGA
TTAACTGCTTAGAAGGTCGCCGTGCCAATCCGCGTACCAAACCACCATTTCCACAAGTAGCTGGTGCATGGGGTCGACCA
ACCGTGGTTAATAACGTTGAAACTTTACATAACGTTTCAGCGATTATCCTACATGGTAGCAAGTGGTATCAAGACCTGCC
AAAAGCCAAAGGTGTAGTCGAAACCCCCGGTACGAAGCTATTTGGCTGTTCTGGTTTGGTCAATGATCCCGGTCTTTGGG
AATTACCTTTTGGTTATACGGCGCGCGAAATCATCGAAGATTTCGCCGGTGGTATGCAGGAAGGTCGCACACTTAAGGCT
TGGTTACCAGGCGGCGCATCAACGGATTTTTTAACCGCTGAACACTTAGATACTGTCGTTGATTTTGACACTATCCAAAA
AGCGGGTAGCCGTATGGGTACGGGTCTGCTTATGGTCGTTGACGAAGAACAAGATATGGTGCCATTGGTACGTAATCTTG
AGATTTTCTTTCAGCGCGAATCGTGTGGTTGGTGTACGCCATGCCGTGATGGTTTGCCGTGGGGCGTCAAAATCCTTACC
GCTATCAATGATGGCGAAGGGCAGATTGGCGATGTTGAAAAGCTAGAAGGTTTAACCCGTGACTTATGGATCGGTAAAAC
CTTTTGCGCGCATGCCCCCGGTGCGATGGAACCACTGATGAGTGCAATTAAATATTTCCGTCCTGAGTTTGATCAAAAGA
TTGCACAGGCGGTTGGTGAAGATGTCATTGAGGCTGCAGCCAATCAACAGCTAGATGTGAAATAA

Upstream 100 bases:

>100_bases
GTTTAGGTAACTGTGACAAAGGTCCTGCGGTATTGATTGATGAAGATACCTATGGTCCTGTCCAGCCTGAAGAAGTCGCC
CAATTGTTGGAGCTATACGC

Downstream 100 bases:

>100_bases
GGAGAGCGGCATGGCAGTCATACATATTGATGGAACCACTGTCGAAGTAGATAGCGCGGACAACTTGCTACAAGCATGCT
TGTCCCTCGGCATTGATGTG

Product: NADH-quinone oxidoreductase subunit F

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F; NDH-1 subunit F; NUO6 [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MRLTVSEQIALRNQGKAPSQLNEQRIPIYGDRATATSETKPLTWRLAHHDAVLDLATYESLRGFEALKIALNQSPDDTLN
TIKEAVVKGRGGAGFPAGIKWSLMAPPDGGPRYLVCNADEMEPGTFKDRLLMERLPLQLIEGMLISAYAIGATAGYIFIR
GEYILAAERLTAAIEELKANNLVGDNILGSDFSFDLHVHTGAGRYICGEETALINCLEGRRANPRTKPPFPQVAGAWGRP
TVVNNVETLHNVSAIILHGSKWYQDLPKAKGVVETPGTKLFGCSGLVNDPGLWELPFGYTAREIIEDFAGGMQEGRTLKA
WLPGGASTDFLTAEHLDTVVDFDTIQKAGSRMGTGLLMVVDEEQDMVPLVRNLEIFFQRESCGWCTPCRDGLPWGVKILT
AINDGEGQIGDVEKLEGLTRDLWIGKTFCAHAPGAMEPLMSAIKYFRPEFDQKIAQAVGEDVIEAAANQQLDVK

Sequences:

>Translated_474_residues
MRLTVSEQIALRNQGKAPSQLNEQRIPIYGDRATATSETKPLTWRLAHHDAVLDLATYESLRGFEALKIALNQSPDDTLN
TIKEAVVKGRGGAGFPAGIKWSLMAPPDGGPRYLVCNADEMEPGTFKDRLLMERLPLQLIEGMLISAYAIGATAGYIFIR
GEYILAAERLTAAIEELKANNLVGDNILGSDFSFDLHVHTGAGRYICGEETALINCLEGRRANPRTKPPFPQVAGAWGRP
TVVNNVETLHNVSAIILHGSKWYQDLPKAKGVVETPGTKLFGCSGLVNDPGLWELPFGYTAREIIEDFAGGMQEGRTLKA
WLPGGASTDFLTAEHLDTVVDFDTIQKAGSRMGTGLLMVVDEEQDMVPLVRNLEIFFQRESCGWCTPCRDGLPWGVKILT
AINDGEGQIGDVEKLEGLTRDLWIGKTFCAHAPGAMEPLMSAIKYFRPEFDQKIAQAVGEDVIEAAANQQLDVK
>Mature_474_residues
MRLTVSEQIALRNQGKAPSQLNEQRIPIYGDRATATSETKPLTWRLAHHDAVLDLATYESLRGFEALKIALNQSPDDTLN
TIKEAVVKGRGGAGFPAGIKWSLMAPPDGGPRYLVCNADEMEPGTFKDRLLMERLPLQLIEGMLISAYAIGATAGYIFIR
GEYILAAERLTAAIEELKANNLVGDNILGSDFSFDLHVHTGAGRYICGEETALINCLEGRRANPRTKPPFPQVAGAWGRP
TVVNNVETLHNVSAIILHGSKWYQDLPKAKGVVETPGTKLFGCSGLVNDPGLWELPFGYTAREIIEDFAGGMQEGRTLKA
WLPGGASTDFLTAEHLDTVVDFDTIQKAGSRMGTGLLMVVDEEQDMVPLVRNLEIFFQRESCGWCTPCRDGLPWGVKILT
AINDGEGQIGDVEKLEGLTRDLWIGKTFCAHAPGAMEPLMSAIKYFRPEFDQKIAQAVGEDVIEAAANQQLDVK

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI260656005, Length=392, Percent_Identity=42.0918367346939, Blast_Score=300, Evalue=1e-81,
Organism=Homo sapiens, GI20149568, Length=392, Percent_Identity=42.0918367346939, Blast_Score=300, Evalue=1e-81,
Organism=Escherichia coli, GI1788620, Length=423, Percent_Identity=64.3026004728132, Blast_Score=540, Evalue=1e-154,
Organism=Caenorhabditis elegans, GI17531727, Length=400, Percent_Identity=41.25, Blast_Score=299, Evalue=2e-81,
Organism=Drosophila melanogaster, GI45550452, Length=392, Percent_Identity=44.3877551020408, Blast_Score=319, Evalue=3e-87,
Organism=Drosophila melanogaster, GI19920794, Length=385, Percent_Identity=42.5974025974026, Blast_Score=304, Evalue=7e-83,
Organism=Drosophila melanogaster, GI24653819, Length=390, Percent_Identity=32.5641025641026, Blast_Score=214, Evalue=7e-56,
Organism=Drosophila melanogaster, GI24653817, Length=348, Percent_Identity=32.4712643678161, Blast_Score=189, Evalue=4e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 51797; Mature: 51797

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLTVSEQIALRNQGKAPSQLNEQRIPIYGDRATATSETKPLTWRLAHHDAVLDLATYES
CEEECCHHHHHHCCCCCCCHHCCCCCCEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHH
LRGFEALKIALNQSPDDTLNTIKEAVVKGRGGAGFPAGIKWSLMAPPDGGPRYLVCNADE
HCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCEEEEECCCC
MEPGTFKDRLLMERLPLQLIEGMLISAYAIGATAGYIFIRGEYILAAERLTAAIEELKAN
CCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEEEEEECCEEEHHHHHHHHHHHHHCC
NLVGDNILGSDFSFDLHVHTGAGRYICGEETALINCLEGRRANPRTKPPFPQVAGAWGRP
CCCCCCCCCCCEEEEEEEECCCCCEEECCCHHHHHHHCCCCCCCCCCCCCHHHCCCCCCC
TVVNNVETLHNVSAIILHGSKWYQDLPKAKGVVETPGTKLFGCSGLVNDPGLWELPFGYT
HHHCCHHHHHCCEEEEEECHHHHHHHHHHCCEEECCCCEEEECCCCCCCCCCEECCCCCC
AREIIEDFAGGMQEGRTLKAWLPGGASTDFLTAEHLDTVVDFDTIQKAGSRMGTGLLMVV
HHHHHHHHHCHHHCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCEEEEEE
DEEQDMVPLVRNLEIFFQRESCGWCTPCRDGLPWGVKILTAINDGEGQIGDVEKLEGLTR
ECCCCHHHHHHCEEEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHCCHH
DLWIGKTFCAHAPGAMEPLMSAIKYFRPEFDQKIAQAVGEDVIEAAANQQLDVK
HHHCCCHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRLTVSEQIALRNQGKAPSQLNEQRIPIYGDRATATSETKPLTWRLAHHDAVLDLATYES
CEEECCHHHHHHCCCCCCCHHCCCCCCEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHH
LRGFEALKIALNQSPDDTLNTIKEAVVKGRGGAGFPAGIKWSLMAPPDGGPRYLVCNADE
HCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCEEEEECCCC
MEPGTFKDRLLMERLPLQLIEGMLISAYAIGATAGYIFIRGEYILAAERLTAAIEELKAN
CCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEEEEEECCEEEHHHHHHHHHHHHHCC
NLVGDNILGSDFSFDLHVHTGAGRYICGEETALINCLEGRRANPRTKPPFPQVAGAWGRP
CCCCCCCCCCCEEEEEEEECCCCCEEECCCHHHHHHHCCCCCCCCCCCCCHHHCCCCCCC
TVVNNVETLHNVSAIILHGSKWYQDLPKAKGVVETPGTKLFGCSGLVNDPGLWELPFGYT
HHHCCHHHHHCCEEEEEECHHHHHHHHHHCCEEECCCCEEEECCCCCCCCCCEECCCCCC
AREIIEDFAGGMQEGRTLKAWLPGGASTDFLTAEHLDTVVDFDTIQKAGSRMGTGLLMVV
HHHHHHHHHCHHHCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCEEEEEE
DEEQDMVPLVRNLEIFFQRESCGWCTPCRDGLPWGVKILTAINDGEGQIGDVEKLEGLTR
ECCCCHHHHHHCEEEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHCCHH
DLWIGKTFCAHAPGAMEPLMSAIKYFRPEFDQKIAQAVGEDVIEAAANQQLDVK
HHHCCCHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7690854; 8157582; 9205837; 9278503; 8366049 [H]