Click here to switch to the map view.

The map label for this gene is nuoE [H]

Identifier: 93005406

GI number: 93005406

Start: 687227

End: 687736

Strand: Direct

Name: nuoE [H]

Synonym: Pcryo_0576

Alternate gene names: 93005406

Gene position: 687227-687736 (Clockwise)

Preceding gene: 93005405

Following gene: 93005407

Centisome position: 22.46

GC content: 43.53

Gene sequence:

>510_bases
ATGAAAATTGTTTCCGATAAAATGCCAAAAGTAGATGTGGCAAGTATCTTAACTGCAGAAGAAATTGCGGCTATTCATGA
GTTTATGCATCATTATCCGCATCCACGAGCAGCGTCTTTAGATTCGCTAAAAATCGTGCAAAAGCGTAACGGCTGGGTTG
ATGATGCGCAAGTGAATGCGATTGCTAATATTTTAAATGTACCTATGACCGATATGGATGGGGTGGCAACCTTCTTTAAC
CGTATCTATCGTCAGCCAGTTGGTCGTCATGTCATCCTTGTTTGTGATTCGGTGGCTTGTTATTTAACAGGCTATGAAGC
ACTGGCAGCTGAATTAAAGTCGCAGCTGGGCATCAACTATGGTCAAACCACTGCTGATGGTCGTTTTACGCTATTGCCGA
TTTGCTGTTTAGGTAACTGTGACAAAGGTCCTGCGGTATTGATTGATGAAGATACCTATGGTCCTGTCCAGCCTGAAGAA
GTCGCCCAATTGTTGGAGCTATACGCATGA

Upstream 100 bases:

>100_bases
CGTTCATTAACATATTATCGCCAAACTGGCACACCAAAACGCTGTCATTGCGCCGTGAGCGCAACCTTAACAGTGGGCTG
AGTGAGGAAAGAAAAAGATT

Downstream 100 bases:

>100_bases
GATTAACTGTTTCAGAGCAGATTGCTCTTCGTAATCAAGGCAAAGCGCCAAGCCAACTTAATGAACAGCGCATTCCTATC
TATGGCGATAGAGCGACTGC

Product: NADH dehydrogenase subunit E

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E; NDH-1 subunit E [H]

Number of amino acids: Translated: 169; Mature: 169

Protein sequence:

>169_residues
MKIVSDKMPKVDVASILTAEEIAAIHEFMHHYPHPRAASLDSLKIVQKRNGWVDDAQVNAIANILNVPMTDMDGVATFFN
RIYRQPVGRHVILVCDSVACYLTGYEALAAELKSQLGINYGQTTADGRFTLLPICCLGNCDKGPAVLIDEDTYGPVQPEE
VAQLLELYA

Sequences:

>Translated_169_residues
MKIVSDKMPKVDVASILTAEEIAAIHEFMHHYPHPRAASLDSLKIVQKRNGWVDDAQVNAIANILNVPMTDMDGVATFFN
RIYRQPVGRHVILVCDSVACYLTGYEALAAELKSQLGINYGQTTADGRFTLLPICCLGNCDKGPAVLIDEDTYGPVQPEE
VAQLLELYA
>Mature_169_residues
MKIVSDKMPKVDVASILTAEEIAAIHEFMHHYPHPRAASLDSLKIVQKRNGWVDDAQVNAIANILNVPMTDMDGVATFFN
RIYRQPVGRHVILVCDSVACYLTGYEALAAELKSQLGINYGQTTADGRFTLLPICCLGNCDKGPAVLIDEDTYGPVQPEE
VAQLLELYA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=157, Percent_Identity=32.484076433121, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI1788621, Length=164, Percent_Identity=54.8780487804878, Blast_Score=201, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17561328, Length=158, Percent_Identity=34.1772151898734, Blast_Score=105, Evalue=9e-24,
Organism=Drosophila melanogaster, GI18859877, Length=151, Percent_Identity=33.112582781457, Blast_Score=97, Evalue=4e-21,
Organism=Drosophila melanogaster, GI21355353, Length=143, Percent_Identity=28.6713286713287, Blast_Score=81, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 18577; Mature: 18577

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS01099 COMPLEX1_24K ; PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVSDKMPKVDVASILTAEEIAAIHEFMHHYPHPRAASLDSLKIVQKRNGWVDDAQVNA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCHHHHHH
IANILNVPMTDMDGVATFFNRIYRQPVGRHVILVCDSVACYLTGYEALAAELKSQLGINY
HHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCC
GQTTADGRFTLLPICCLGNCDKGPAVLIDEDTYGPVQPEEVAQLLELYA
CCCCCCCCEEEEEEEECCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKIVSDKMPKVDVASILTAEEIAAIHEFMHHYPHPRAASLDSLKIVQKRNGWVDDAQVNA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCHHHHHH
IANILNVPMTDMDGVATFFNRIYRQPVGRHVILVCDSVACYLTGYEALAAELKSQLGINY
HHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCC
GQTTADGRFTLLPICCLGNCDKGPAVLIDEDTYGPVQPEEVAQLLELYA
CCCCCCCCEEEEEEEECCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]