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The map label for this gene is trpA [H]

Identifier: 93005298

GI number: 93005298

Start: 574322

End: 575140

Strand: Direct

Name: trpA [H]

Synonym: Pcryo_0468

Alternate gene names: 93005298

Gene position: 574322-575140 (Clockwise)

Preceding gene: 93005297

Following gene: 93005299

Centisome position: 18.77

GC content: 45.3

Gene sequence:

>819_bases
ATGACCCGAATTGAAAGCACCTTTGAAACCCTAAAAGCACAAAATAAAAAAGCACTCATCCCTTATGTGATGGCAGGCGA
TCCCAATCCAAGCAACTTTGTTGGCTTGCTGCATGATTTAGTCAAGCATGGCGCAGATATGATTGAAGTGGGCTTACCAT
TTTCTGACCCAATGGCAGATGGTCCTACAGTAGCATTGGCAGGCGAGCGTGCATTAGCAGCGGGTACGAGCACTCGTGAT
GCACTAAAGATGGTCAAAGAGTTCCGTCAGCAAGATACGCAGACGCCAATTATCCTGATGGGTTACCTTAATCCAGTAGA
GATTATCGGTTATGATAACTTTGTCTCTTTATGTGAGCAGTCAGGCGTCGATGGTATTTTGATGGTTGATTTGCCGCCAG
CAGAAGCGGGTAGCTTTACCCAGCATTTGACTGAGCACGCGATGAATGAGATTTTCTTATTATCACCAACTACGCTGGCT
GAACGTCGTCAGCAAGTATTGACCCATTGCGGTGGTTATATCTACTACGTGTCTTTAAAAGGCGTGACGGGTTCAGCAAC
GCTAGATACTGACGATGTTGCAAAGCAAGTACAGGCCATTAAAGCAGAGACAGATTTGCCAGTATGTGTGGGCTTTGGTA
TTCGTGATGCAACTTCTGCCAAAGCAATTGGTGCTCATGCGGATGGTATTATCGTCGGTAGTGCATTGGTACAGAACTTT
GCTGATATCGATGCCAATGATATCACTGCAGTCGCTAATGCTCAGCAAAAAATTATGGCAAAAATGGATGAGTTACGCGG
CGCACTCGATAGTTTATAA

Upstream 100 bases:

>100_bases
TAACAACTGGATTATTCCTTGGAAGAAAAATTACTGATTTGACCCGTTATTATTAAATAAAGGGTCAAATGAGCCAGTTT
ATAATAATTAAGAGACCACT

Downstream 100 bases:

>100_bases
GCACCCGCTCAAACAGTTGGGCAAGTTATCGTCACAATATTTATGAGGGTATTTATAACAATGTTTATAAAGACACAGGC
AAATGGCTTTGCTAAAGTTA

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MTRIESTFETLKAQNKKALIPYVMAGDPNPSNFVGLLHDLVKHGADMIEVGLPFSDPMADGPTVALAGERALAAGTSTRD
ALKMVKEFRQQDTQTPIILMGYLNPVEIIGYDNFVSLCEQSGVDGILMVDLPPAEAGSFTQHLTEHAMNEIFLLSPTTLA
ERRQQVLTHCGGYIYYVSLKGVTGSATLDTDDVAKQVQAIKAETDLPVCVGFGIRDATSAKAIGAHADGIIVGSALVQNF
ADIDANDITAVANAQQKIMAKMDELRGALDSL

Sequences:

>Translated_272_residues
MTRIESTFETLKAQNKKALIPYVMAGDPNPSNFVGLLHDLVKHGADMIEVGLPFSDPMADGPTVALAGERALAAGTSTRD
ALKMVKEFRQQDTQTPIILMGYLNPVEIIGYDNFVSLCEQSGVDGILMVDLPPAEAGSFTQHLTEHAMNEIFLLSPTTLA
ERRQQVLTHCGGYIYYVSLKGVTGSATLDTDDVAKQVQAIKAETDLPVCVGFGIRDATSAKAIGAHADGIIVGSALVQNF
ADIDANDITAVANAQQKIMAKMDELRGALDSL
>Mature_271_residues
TRIESTFETLKAQNKKALIPYVMAGDPNPSNFVGLLHDLVKHGADMIEVGLPFSDPMADGPTVALAGERALAAGTSTRDA
LKMVKEFRQQDTQTPIILMGYLNPVEIIGYDNFVSLCEQSGVDGILMVDLPPAEAGSFTQHLTEHAMNEIFLLSPTTLAE
RRQQVLTHCGGYIYYVSLKGVTGSATLDTDDVAKQVQAIKAETDLPVCVGFGIRDATSAKAIGAHADGIIVGSALVQNFA
DIDANDITAVANAQQKIMAKMDELRGALDSL

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate [H]

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family [H]

Homologues:

Organism=Escherichia coli, GI1787514, Length=214, Percent_Identity=36.4485981308411, Blast_Score=143, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6321412, Length=239, Percent_Identity=35.9832635983264, Blast_Score=160, Evalue=2e-40,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028 [H]

Pfam domain/function: PF00290 Trp_syntA [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 29052; Mature: 28920

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRIESTFETLKAQNKKALIPYVMAGDPNPSNFVGLLHDLVKHGADMIEVGLPFSDPMAD
CCCHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
GPTVALAGERALAAGTSTRDALKMVKEFRQQDTQTPIILMGYLNPVEIIGYDNFVSLCEQ
CCEEEEECCCCEECCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCHHHHHHHHH
SGVDGILMVDLPPAEAGSFTQHLTEHAMNEIFLLSPTTLAERRQQVLTHCGGYIYYVSLK
CCCCEEEEEECCCCCCCHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHCCCEEEEEEEE
GVTGSATLDTDDVAKQVQAIKAETDLPVCVGFGIRDATSAKAIGAHADGIIVGSALVQNF
CCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHCCCCCCCEEEHHHHHHHH
ADIDANDITAVANAQQKIMAKMDELRGALDSL
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TRIESTFETLKAQNKKALIPYVMAGDPNPSNFVGLLHDLVKHGADMIEVGLPFSDPMAD
CCHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
GPTVALAGERALAAGTSTRDALKMVKEFRQQDTQTPIILMGYLNPVEIIGYDNFVSLCEQ
CCEEEEECCCCEECCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCHHHHHHHHH
SGVDGILMVDLPPAEAGSFTQHLTEHAMNEIFLLSPTTLAERRQQVLTHCGGYIYYVSLK
CCCCEEEEEECCCCCCCHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHCCCEEEEEEEE
GVTGSATLDTDDVAKQVQAIKAETDLPVCVGFGIRDATSAKAIGAHADGIIVGSALVQNF
CCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHCCCCCCCEEEHHHHHHHH
ADIDANDITAVANAQQKIMAKMDELRGALDSL
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA