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The map label for this gene is gltS [H]

Identifier: 93005150

GI number: 93005150

Start: 387354

End: 388628

Strand: Reverse

Name: gltS [H]

Synonym: Pcryo_0319

Alternate gene names: 93005150

Gene position: 388628-387354 (Counterclockwise)

Preceding gene: 93005151

Following gene: 93005146

Centisome position: 12.7

GC content: 44.55

Gene sequence:

>1275_bases
ATGGAAATCACCCTAAACGGCTATTACACCCTGATATTAGCCACATTAGTACTTTTGCTTGGACGCTTCTTAGTCAAAAG
AATTAAGTTTTTAGAAGACTTCAATATTCCAGAACCAGTCGCAGGCGGTTTGGTCGCAGCGATTGTCGTTTATATTCTTA
ATATTGTTTGGGGCTATAGCTTTAATTTCCATCAAGGACTGCAAACAGCGACCATGCTGATGTTTTTTGCCTCTATCGGG
CTTAGCGCTGATTTTGGTCGGTTAAAAGCAGGTGGCTCACCCTTGCTGATTTTCACCATTATCGTGTCTGTTTTTATCAT
TTTGCAAGACATCCTTGGTGTTGCAATGGCAAGCGCACTTGGGCTTGATCCTTTGCTTGGCTTGGTGACCGGTTCAATCG
CTTTGACTGGTGGACATGGTACTGCTGGTGCTTGGGGCATTACTTTGGAGGAGCAGTATGGTGTGGTTGGCGCGACGACG
CTGGGTATCGCAGTTGCCACTTATGGTTTAGTAGCAGGTGGTATTATCGGTGGTCCTGTTGCCCGTCGCTTGATTAAAAA
GCTTGGTCTCAAGCCTACAGCAGACAATCCAAACCCAAGTGATGTTGAAAAACTGGCAAGTGAGCAGTCGCTCTATAGCA
CCAAGCACAGCGAAGAGGAAGTTCACAGCAATAAGGAGATGTTTGAAAAACCGGATAATATCCGTCTGATTACGGCATCG
TCTACCATTGAATCATTGGCACTGTTTGCCGCAGCATTGGCATTTGCTGATCTCATGACTTTGGTAGCACAGGATACATG
GTTTGAGTTACCGACCTTCGTATGGGCATTAGCAGGTGGTGTGATTTTGCGCAATACCTTAACCATGGTATTTAACTTTG
ATATGTTTGACCGTGCGATTGACGTCATCGGTAACGCTTCTTTGAGTCTTTTCTTAGCGATGGCATTATTGTCATTAAAG
CTGTGGCAATTGACAGATTTGGCAGGTCCGGTATTGATCATCTTATTGTCACAAACTGTTCTAATGATTGGCTATGCTTA
CTTTGTGACCTTCAAACTCATGGGCAAAGACTATGATGCAGCTGTCTTGTCAGCAGGACACTGTGGTTTCGGTATGGGTG
CAACTCCTACAGCCATTGCCAATATGCAGGCGGTCACCGATCGTTACCTACCATCACATAAGGCATTTTTAATTGTACCT
ATGGTTGGTGCATTCTTCGTTGATATCGTTAACGCGACGATCTTGCAGATATTCACCAAATTGCCATTTATGTGA

Upstream 100 bases:

>100_bases
GATTTGTCTTAGTCGCTAGTAAGAGAAGAATCTAAATTCGAGATAAAAACAGATTTATCCCGAATTCAGGTTAATTTATA
TTTATAGAAAGGATTAACAT

Downstream 100 bases:

>100_bases
TTTGATGAAATAAAAAGCTGATTCATATACTCACAAAAAAACCGCCTAGCAAAAGCTAAGCGGTTTTTTTATTTCTGACT
TATAAAACCAATTAATTAGA

Product: sodium/glutamate symporter

Products: Na (I) [Cytoplasm]; L-glutamate [Cytoplasm] [C]

Alternate protein names: Glutamate permease [H]

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MEITLNGYYTLILATLVLLLGRFLVKRIKFLEDFNIPEPVAGGLVAAIVVYILNIVWGYSFNFHQGLQTATMLMFFASIG
LSADFGRLKAGGSPLLIFTIIVSVFIILQDILGVAMASALGLDPLLGLVTGSIALTGGHGTAGAWGITLEEQYGVVGATT
LGIAVATYGLVAGGIIGGPVARRLIKKLGLKPTADNPNPSDVEKLASEQSLYSTKHSEEEVHSNKEMFEKPDNIRLITAS
STIESLALFAAALAFADLMTLVAQDTWFELPTFVWALAGGVILRNTLTMVFNFDMFDRAIDVIGNASLSLFLAMALLSLK
LWQLTDLAGPVLIILLSQTVLMIGYAYFVTFKLMGKDYDAAVLSAGHCGFGMGATPTAIANMQAVTDRYLPSHKAFLIVP
MVGAFFVDIVNATILQIFTKLPFM

Sequences:

>Translated_424_residues
MEITLNGYYTLILATLVLLLGRFLVKRIKFLEDFNIPEPVAGGLVAAIVVYILNIVWGYSFNFHQGLQTATMLMFFASIG
LSADFGRLKAGGSPLLIFTIIVSVFIILQDILGVAMASALGLDPLLGLVTGSIALTGGHGTAGAWGITLEEQYGVVGATT
LGIAVATYGLVAGGIIGGPVARRLIKKLGLKPTADNPNPSDVEKLASEQSLYSTKHSEEEVHSNKEMFEKPDNIRLITAS
STIESLALFAAALAFADLMTLVAQDTWFELPTFVWALAGGVILRNTLTMVFNFDMFDRAIDVIGNASLSLFLAMALLSLK
LWQLTDLAGPVLIILLSQTVLMIGYAYFVTFKLMGKDYDAAVLSAGHCGFGMGATPTAIANMQAVTDRYLPSHKAFLIVP
MVGAFFVDIVNATILQIFTKLPFM
>Mature_424_residues
MEITLNGYYTLILATLVLLLGRFLVKRIKFLEDFNIPEPVAGGLVAAIVVYILNIVWGYSFNFHQGLQTATMLMFFASIG
LSADFGRLKAGGSPLLIFTIIVSVFIILQDILGVAMASALGLDPLLGLVTGSIALTGGHGTAGAWGITLEEQYGVVGATT
LGIAVATYGLVAGGIIGGPVARRLIKKLGLKPTADNPNPSDVEKLASEQSLYSTKHSEEEVHSNKEMFEKPDNIRLITAS
STIESLALFAAALAFADLMTLVAQDTWFELPTFVWALAGGVILRNTLTMVFNFDMFDRAIDVIGNASLSLFLAMALLSLK
LWQLTDLAGPVLIILLSQTVLMIGYAYFVTFKLMGKDYDAAVLSAGHCGFGMGATPTAIANMQAVTDRYLPSHKAFLIVP
MVGAFFVDIVNATILQIFTKLPFM

Specific function: Catalyzes the sodium-dependent uptake of extracellular glutamate [H]

COG id: COG0786

COG function: function code E; Na+/glutamate symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gltS symporter family [H]

Homologues:

Organism=Escherichia coli, GI1790085, Length=402, Percent_Identity=50.9950248756219, Blast_Score=346, Evalue=2e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004445 [H]

Pfam domain/function: PF03616 Glt_symporter [H]

EC number: NA

Molecular weight: Translated: 45476; Mature: 45476

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00134 TRYPSIN_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEITLNGYYTLILATLVLLLGRFLVKRIKFLEDFNIPEPVAGGLVAAIVVYILNIVWGYS
CEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC
FNFHQGLQTATMLMFFASIGLSADFGRLKAGGSPLLIFTIIVSVFIILQDILGVAMASAL
CCHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GLDPLLGLVTGSIALTGGHGTAGAWGITLEEQYGVVGATTLGIAVATYGLVAGGIIGGPV
CHHHHHHHHHCCEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCHHH
ARRLIKKLGLKPTADNPNPSDVEKLASEQSLYSTKHSEEEVHSNKEMFEKPDNIRLITAS
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHCCCCCEEEEEEC
STIESLALFAAALAFADLMTLVAQDTWFELPTFVWALAGGVILRNTLTMVFNFDMFDRAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
DVIGNASLSLFLAMALLSLKLWQLTDLAGPVLIILLSQTVLMIGYAYFVTFKLMGKDYDA
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
AVLSAGHCGFGMGATPTAIANMQAVTDRYLPSHKAFLIVPMVGAFFVDIVNATILQIFTK
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
LPFM
CCCC
>Mature Secondary Structure
MEITLNGYYTLILATLVLLLGRFLVKRIKFLEDFNIPEPVAGGLVAAIVVYILNIVWGYS
CEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC
FNFHQGLQTATMLMFFASIGLSADFGRLKAGGSPLLIFTIIVSVFIILQDILGVAMASAL
CCHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GLDPLLGLVTGSIALTGGHGTAGAWGITLEEQYGVVGATTLGIAVATYGLVAGGIIGGPV
CHHHHHHHHHCCEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCHHH
ARRLIKKLGLKPTADNPNPSDVEKLASEQSLYSTKHSEEEVHSNKEMFEKPDNIRLITAS
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHCCCCCEEEEEEC
STIESLALFAAALAFADLMTLVAQDTWFELPTFVWALAGGVILRNTLTMVFNFDMFDRAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
DVIGNASLSLFLAMALLSLKLWQLTDLAGPVLIILLSQTVLMIGYAYFVTFKLMGKDYDA
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
AVLSAGHCGFGMGATPTAIANMQAVTDRYLPSHKAFLIVPMVGAFFVDIVNATILQIFTK
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
LPFM
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-glutamate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-glutamate [Periplasm] = Na (I) [Cytoplasm] + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]