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The map label for this gene is rluE [H]

Identifier: 93005144

GI number: 93005144

Start: 378549

End: 379175

Strand: Reverse

Name: rluE [H]

Synonym: Pcryo_0313

Alternate gene names: 93005144

Gene position: 379175-378549 (Counterclockwise)

Preceding gene: 93005146

Following gene: 93005143

Centisome position: 12.39

GC content: 46.09

Gene sequence:

>627_bases
ATGTCCAGCTCTAGCCTGATTTTGTTTAATAAGCCTTACGGAGTACAAAGTCAATTCCGTGATGAAAGCAATAATGACCA
TACTACTCTATCAGAATATTTTACGGACAAGTCACTCCGTATCGCTGGTCGACTTGATGCTACCTCAGAGGGGCTACTGA
TTTTGACCAGTGATGGACGCATTAATAAAGCCATCACGCAGCCACCATCTGCAAAGGGTTTTGCGCAAAACAAACAAAAA
CAAGGTAAGACTTACTTGGTGCAAGTAGAAGGCATGGTCACTTCTGTCCAATTAAAGGCGCTGGCGTCAGGCGTGGTCTT
GAAAGACGGTAAGACATTGCCTGCCTCTGCAGTATTGGTGGATGAAGTCGACTTGCCGATTACATTATGGGAGCGTGAGC
CGCCTATTCGTGAGCGTAAAAATATCCCAACGTCATGGTTGATGCTGACTATTTATGAAGGCAAAAATCGCCAAGTGAGG
CGTATGACTGCGCATGTAGGGCTGCCTTGCTTACGCTTGATACGCTGGTCAGTGGCAGGATTTGAGCTTGGAGAGTTGGG
CGTTGGTGAGTTTGTACGCATTCATTTAAATAGTGAGCAATGCCGAAAACTTGGCGTCATAGGTTAG

Upstream 100 bases:

>100_bases
TAAGGTAAAACATGCCTCTACCTTTAGGCTTTACTCGTATATTAATTCTTATAGTATATGTCCTGCTATCCGCCATTTTA
TTTTTGGGAAACGCTATTTT

Downstream 100 bases:

>100_bases
CTATAATAATACCTGAGTAAAGCTGTACTTAAGATAGCTTTTTTATTTAAGATCACTATATTACGACATCTTAAACATAG
CGTCTCGAAAGAGTAAGATT

Product: pseudouridine synthase Rsu

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 208; Mature: 207

Protein sequence:

>208_residues
MSSSSLILFNKPYGVQSQFRDESNNDHTTLSEYFTDKSLRIAGRLDATSEGLLILTSDGRINKAITQPPSAKGFAQNKQK
QGKTYLVQVEGMVTSVQLKALASGVVLKDGKTLPASAVLVDEVDLPITLWEREPPIRERKNIPTSWLMLTIYEGKNRQVR
RMTAHVGLPCLRLIRWSVAGFELGELGVGEFVRIHLNSEQCRKLGVIG

Sequences:

>Translated_208_residues
MSSSSLILFNKPYGVQSQFRDESNNDHTTLSEYFTDKSLRIAGRLDATSEGLLILTSDGRINKAITQPPSAKGFAQNKQK
QGKTYLVQVEGMVTSVQLKALASGVVLKDGKTLPASAVLVDEVDLPITLWEREPPIRERKNIPTSWLMLTIYEGKNRQVR
RMTAHVGLPCLRLIRWSVAGFELGELGVGEFVRIHLNSEQCRKLGVIG
>Mature_207_residues
SSSSLILFNKPYGVQSQFRDESNNDHTTLSEYFTDKSLRIAGRLDATSEGLLILTSDGRINKAITQPPSAKGFAQNKQKQ
GKTYLVQVEGMVTSVQLKALASGVVLKDGKTLPASAVLVDEVDLPITLWEREPPIRERKNIPTSWLMLTIYEGKNRQVRR
MTAHVGLPCLRLIRWSVAGFELGELGVGEFVRIHLNSEQCRKLGVIG

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=188, Percent_Identity=53.1914893617021, Blast_Score=189, Evalue=1e-49,
Organism=Escherichia coli, GI1788510, Length=190, Percent_Identity=33.1578947368421, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 23095; Mature: 22964

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSSLILFNKPYGVQSQFRDESNNDHTTLSEYFTDKSLRIAGRLDATSEGLLILTSDGR
CCCCCEEEEECCCCCCHHHCCCCCCCCCCHHHHHCCCCEEEEEECCCCCCCEEEEECCCC
INKAITQPPSAKGFAQNKQKQGKTYLVQVEGMVTSVQLKALASGVVLKDGKTLPASAVLV
CCHHCCCCCCCCCCHHCHHHCCCEEEEEECCEEEEEEHHHHHCCEEEECCCCCCCCEEEE
DEVDLPITLWEREPPIRERKNIPTSWLMLTIYEGKNRQVRRMTAHVGLPCLRLIRWSVAG
ECCCCCEEEECCCCCHHHHHCCCCEEEEEEEEECCCCEEEHHHHHCCCHHHHHHHHHHCC
FELGELGVGEFVRIHLNSEQCRKLGVIG
CEECCCCCCEEEEEEECHHHHHHCCCCC
>Mature Secondary Structure 
SSSSLILFNKPYGVQSQFRDESNNDHTTLSEYFTDKSLRIAGRLDATSEGLLILTSDGR
CCCCEEEEECCCCCCHHHCCCCCCCCCCHHHHHCCCCEEEEEECCCCCCCEEEEECCCC
INKAITQPPSAKGFAQNKQKQGKTYLVQVEGMVTSVQLKALASGVVLKDGKTLPASAVLV
CCHHCCCCCCCCCCHHCHHHCCCEEEEEECCEEEEEEHHHHHCCEEEECCCCCCCCEEEE
DEVDLPITLWEREPPIRERKNIPTSWLMLTIYEGKNRQVRRMTAHVGLPCLRLIRWSVAG
ECCCCCEEEECCCCCHHHHHCCCCEEEEEEEEECCCCEEEHHHHHCCCHHHHHHHHHHCC
FELGELGVGEFVRIHLNSEQCRKLGVIG
CEECCCCCCEEEEEEECHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]