Click here to switch to the map view.

The map label for this gene is pldA [C]

Identifier: 93005091

GI number: 93005091

Start: 308802

End: 310220

Strand: Direct

Name: pldA [C]

Synonym: Pcryo_0260

Alternate gene names: 93005091

Gene position: 308802-310220 (Clockwise)

Preceding gene: 93005090

Following gene: 93005092

Centisome position: 10.09

GC content: 42.14

Gene sequence:

>1419_bases
ATGTTTTCCCATATTACTTCGCAGCGCCCCTCTGTAAATAAACTTGAAACGCCAAAAAAAATGATGCTTCGAACGACTAA
ATGCTCTCTACACATCGCTGTTGGTATAGCGCTCACTTGTTTCACAGTACAAGCTCAGGCTGCTGATACAGCTCAGACTA
AGAGCTTACAGCCTATAAATACCAGTATCGCTGTGCAAAATAGCAATGCAGTAAGCGAAGATTTTATCGCTCAGCAAGCT
GCTCTGTTCTTTGAATGTACTCAAGTACAGACCAGTTCTGCCCGTTTGGCATGCTTTGATAAGGTTGCAGAACAAGGCAA
AACGCCAAGTTATGTCACAACCAAACAGCCAGTAGATTTGGCAAAAACGGTTATAAGTACGCTGTCAGGCAACCCTCAAG
TGATACTGGCTGAAGAAACCAGTACCATTACTGCCAATGGCAATGTCATTGCTAAAAAGGTTTCGAGCAATTATCCTCAA
GATATTGAAACAAAACTGCCCGCTGAGACTGAAAGTTTAGATACGGTTGGACTGACGCAAAGAGAAGCAGAGGTATTGGA
AAGTGTCGGCGTGACTCAAGCAGATATTGAAAAATATACGCCGCTAAGCTTAGCATATGATCTTGATAAAAACAGTGAAC
GCGGTACATGGACAGTCAGACCTTACCGTCCAACCTATGTTATGCCACTGTTTTATACCTTTGATCCCAATTTAAGCCCA
AGTACACCCACTCGACCTCAACCAGAGAAGCCTTTTACGTCTAACGACACTCGTAATACGGATCTAAAGTTTCAACTATC
ATTAAAGACTAAAGTTGCAGAAGATTTGTTTAATACCAATGCTGATTTGTGGTTTGGTTATACTCAAGAGTCGCATTGGC
AAGTTTATAATGAAGACAATTCACGACCATTTCGTGCAACCGATTATCAGCCAGAAATATTTTTGACCCAGCCTGTGACA
GCAAATCTGCCTTTTGGTGGACGACTGCGCATGCTCGGAGCAGGTGCTATTCACCACTCAAATGGTCAAGATGATCCATT
ATCGCGCTCGTGGAACCGTGCTTATGTGATGGCAGGGGCAGAATGGGGCAAGTTATCTGTCATACCGCGATTCTGGTTAC
GAGTGAACAATGAAAATGATAGCAGTGAAGACAATCCAGATATTGAAGATTTTATGGGTTATGGTGATATCAAGTTCTTA
TATGATTTACCAAATCAACAAAGCCTAAGCGGTACGTTGCGCTACAATCCAGGCACTAATAAAGGCGCTGCGCAAATCGA
TTATATTTATCCGTTATCTAAAAACGTTAATGGCTTTGTACAGCTGTTCCAAGGGTATGGCGAATCTATTGTTGATTATA
ACCATGAAAATACGGCTATCGGCTTTGGTATCGTACTCAATGATTGGAAAGGGCTGTAA

Upstream 100 bases:

>100_bases
GTATTGATTTTTTATGCCAGTTTAGCGTCAATGCTAATTGTTATATCAATCACCTCATATATTCTATTGACAGTAATGAT
CAGTTGAATAAAGGATGCTT

Downstream 100 bases:

>100_bases
CATTAGCGCTTTAAATATCGGTGCCTATAGTGACAGCAAGTCATGCGTCACTTAGCGCCTTATCAAGCCGGACTATGGCT
GGCAGAGTATATAAACTTAC

Product: phospholipase A1

Products: NA

Alternate protein names: Phosphatidylcholine 1-acylhydrolase [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MFSHITSQRPSVNKLETPKKMMLRTTKCSLHIAVGIALTCFTVQAQAADTAQTKSLQPINTSIAVQNSNAVSEDFIAQQA
ALFFECTQVQTSSARLACFDKVAEQGKTPSYVTTKQPVDLAKTVISTLSGNPQVILAEETSTITANGNVIAKKVSSNYPQ
DIETKLPAETESLDTVGLTQREAEVLESVGVTQADIEKYTPLSLAYDLDKNSERGTWTVRPYRPTYVMPLFYTFDPNLSP
STPTRPQPEKPFTSNDTRNTDLKFQLSLKTKVAEDLFNTNADLWFGYTQESHWQVYNEDNSRPFRATDYQPEIFLTQPVT
ANLPFGGRLRMLGAGAIHHSNGQDDPLSRSWNRAYVMAGAEWGKLSVIPRFWLRVNNENDSSEDNPDIEDFMGYGDIKFL
YDLPNQQSLSGTLRYNPGTNKGAAQIDYIYPLSKNVNGFVQLFQGYGESIVDYNHENTAIGFGIVLNDWKGL

Sequences:

>Translated_472_residues
MFSHITSQRPSVNKLETPKKMMLRTTKCSLHIAVGIALTCFTVQAQAADTAQTKSLQPINTSIAVQNSNAVSEDFIAQQA
ALFFECTQVQTSSARLACFDKVAEQGKTPSYVTTKQPVDLAKTVISTLSGNPQVILAEETSTITANGNVIAKKVSSNYPQ
DIETKLPAETESLDTVGLTQREAEVLESVGVTQADIEKYTPLSLAYDLDKNSERGTWTVRPYRPTYVMPLFYTFDPNLSP
STPTRPQPEKPFTSNDTRNTDLKFQLSLKTKVAEDLFNTNADLWFGYTQESHWQVYNEDNSRPFRATDYQPEIFLTQPVT
ANLPFGGRLRMLGAGAIHHSNGQDDPLSRSWNRAYVMAGAEWGKLSVIPRFWLRVNNENDSSEDNPDIEDFMGYGDIKFL
YDLPNQQSLSGTLRYNPGTNKGAAQIDYIYPLSKNVNGFVQLFQGYGESIVDYNHENTAIGFGIVLNDWKGL
>Mature_472_residues
MFSHITSQRPSVNKLETPKKMMLRTTKCSLHIAVGIALTCFTVQAQAADTAQTKSLQPINTSIAVQNSNAVSEDFIAQQA
ALFFECTQVQTSSARLACFDKVAEQGKTPSYVTTKQPVDLAKTVISTLSGNPQVILAEETSTITANGNVIAKKVSSNYPQ
DIETKLPAETESLDTVGLTQREAEVLESVGVTQADIEKYTPLSLAYDLDKNSERGTWTVRPYRPTYVMPLFYTFDPNLSP
STPTRPQPEKPFTSNDTRNTDLKFQLSLKTKVAEDLFNTNADLWFGYTQESHWQVYNEDNSRPFRATDYQPEIFLTQPVT
ANLPFGGRLRMLGAGAIHHSNGQDDPLSRSWNRAYVMAGAEWGKLSVIPRFWLRVNNENDSSEDNPDIEDFMGYGDIKFL
YDLPNQQSLSGTLRYNPGTNKGAAQIDYIYPLSKNVNGFVQLFQGYGESIVDYNHENTAIGFGIVLNDWKGL

Specific function: Hydrolysis of phosphatidylcholine with phospholipase A2 (EC 3.1.1.4) and phospholipase A1 (EC 3.1.1.32) activities [H]

COG id: COG2829

COG function: function code M; Outer membrane phospholipase A

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein. Note=One of the very few enzymes located there (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phospholipase A1 family [H]

Homologues:

Organism=Escherichia coli, GI2367300, Length=242, Percent_Identity=38.4297520661157, Blast_Score=152, Evalue=4e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003187 [H]

Pfam domain/function: PF02253 PLA1 [H]

EC number: =3.1.1.32; =3.1.1.4 [H]

Molecular weight: Translated: 52602; Mature: 52602

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSHITSQRPSVNKLETPKKMMLRTTKCSLHIAVGIALTCFTVQAQAADTAQTKSLQPIN
CCCCCCCCCCCCCCCCCCHHHHEEEECEEEEEEEEEEEEEEEEEECCCCCHHHCCCCCCC
TSIAVQNSNAVSEDFIAQQAALFFECTQVQTSSARLACFDKVAEQGKTPSYVTTKQPVDL
CEEEEECCCCCHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHCCCCCCEEECCCCHHH
AKTVISTLSGNPQVILAEETSTITANGNVIAKKVSSNYPQDIETKLPAETESLDTVGLTQ
HHHHHHHHCCCCCEEEEECCCEEEECCCEEEEECCCCCCCCHHHCCCCCCCCCCCCCCCH
REAEVLESVGVTQADIEKYTPLSLAYDLDKNSERGTWTVRPYRPTYVMPLFYTFDPNLSP
HHHHHHHHCCCCHHHHHHCCCEEEEEECCCCCCCCEEEECCCCCEEEEEEEEEECCCCCC
STPTRPQPEKPFTSNDTRNTDLKFQLSLKTKVAEDLFNTNADLWFGYTQESHWQVYNEDN
CCCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHCCCCCEEEEECCCCCEEEEECCC
SRPFRATDYQPEIFLTQPVTANLPFGGRLRMLGAGAIHHSNGQDDPLSRSWNRAYVMAGA
CCCEEECCCCCCEEEECCEEECCCCCCEEEEEEECEEECCCCCCCCCCCCCCEEEEEECC
EWGKLSVIPRFWLRVNNENDSSEDNPDIEDFMGYGDIKFLYDLPNQQSLSGTLRYNPGTN
CCCEEEECEEEEEEECCCCCCCCCCCCHHHHCCCCCEEEEEECCCCCCCCEEEEECCCCC
KGAAQIDYIYPLSKNVNGFVQLFQGYGESIVDYNHENTAIGFGIVLNDWKGL
CCCEEEEEEEECCCCCHHHHHHHHHCCCHHEECCCCCCEEEEEEEECCCCCC
>Mature Secondary Structure
MFSHITSQRPSVNKLETPKKMMLRTTKCSLHIAVGIALTCFTVQAQAADTAQTKSLQPIN
CCCCCCCCCCCCCCCCCCHHHHEEEECEEEEEEEEEEEEEEEEEECCCCCHHHCCCCCCC
TSIAVQNSNAVSEDFIAQQAALFFECTQVQTSSARLACFDKVAEQGKTPSYVTTKQPVDL
CEEEEECCCCCHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHCCCCCCEEECCCCHHH
AKTVISTLSGNPQVILAEETSTITANGNVIAKKVSSNYPQDIETKLPAETESLDTVGLTQ
HHHHHHHHCCCCCEEEEECCCEEEECCCEEEEECCCCCCCCHHHCCCCCCCCCCCCCCCH
REAEVLESVGVTQADIEKYTPLSLAYDLDKNSERGTWTVRPYRPTYVMPLFYTFDPNLSP
HHHHHHHHCCCCHHHHHHCCCEEEEEECCCCCCCCEEEECCCCCEEEEEEEEEECCCCCC
STPTRPQPEKPFTSNDTRNTDLKFQLSLKTKVAEDLFNTNADLWFGYTQESHWQVYNEDN
CCCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHCCCCCEEEEECCCCCEEEEECCC
SRPFRATDYQPEIFLTQPVTANLPFGGRLRMLGAGAIHHSNGQDDPLSRSWNRAYVMAGA
CCCEEECCCCCCEEEECCEEECCCCCCEEEEEEECEEECCCCCCCCCCCCCCEEEEEECC
EWGKLSVIPRFWLRVNNENDSSEDNPDIEDFMGYGDIKFLYDLPNQQSLSGTLRYNPGTN
CCCEEEECEEEEEEECCCCCCCCCCCCHHHHCCCCCEEEEEECCCCCCCCEEEEECCCCC
KGAAQIDYIYPLSKNVNGFVQLFQGYGESIVDYNHENTAIGFGIVLNDWKGL
CCCEEEEEEEECCCCCHHHHHHHHHCCCHHEECCCCCCEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]