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The map label for this gene is alkR [H]

Identifier: 93005085

GI number: 93005085

Start: 300431

End: 301366

Strand: Direct

Name: alkR [H]

Synonym: Pcryo_0254

Alternate gene names: 93005085

Gene position: 300431-301366 (Clockwise)

Preceding gene: 93005084

Following gene: 93005086

Centisome position: 9.82

GC content: 44.55

Gene sequence:

>936_bases
ATGGACGCATTAAGCGTGTTACTACAAAATGTACATTTATTCGATACGAAATACTATCGCTTAAATGGTACAGGCAATTG
GTCTTACTCACTCACTAGACAAGATACGATTGTGTTTTATTTGGTGATGTCTGGTAGCTTTTGTATTGATATGGGCAGTG
GACTCAGAGAAGCGCATGCTGGCGACATTATTATGATTCCTAGCGCGCACAATCATGTCAGTTACGCGCTCAACCATCAT
GGTGAGAGTGCCAAACCTTTGGATGAGTTGCTTACTAATTGCACGGAACAAGTGCTTGATATTCAGGGCGATGGTGATGC
CCAATCATCTTTGATACTGATTGAATGCAAGTACGATAAAGAGATGATTCGTCCTTTATTATCAGTGCTACCAACGATAT
TGCCTGAAATTAACGATGAATCTGATGGTCGGTTCGAGGTGATTGATGTTGAGATTCGACTGCTGACGTTAGAAGCTGAG
CATGAGCGGATGGGTAAGACGGCGATTATCAATCATTGGGCAAGTATTATGATGATTGAGTGTTTGCGGGTTTATATCGA
AAGTTTGCCTGAAGCCACGGACAGTTGGCTAAAAGCCATGAAAGACCCGTTTTTAACCAAGGCATTGGTCGCCATGCACG
AAAATCCAAGCCAGAACTGGAGCATTCATGAGCTTGCTGAAGTTGCAGGTATGTCACGCTCGAGTTTTGCGCAGCGTTTC
AAAGACGTGGTTGGTATGCCGCCGTTGACCTATCTGATTGACTACCGTTTGCGTCTGGCGGCACGTTATTTGCGCTTGCA
GCAAAATAGTATCAGCCGTATTAGTGAGCTAGTGGGCTATGCCTCAGACTCAACCTTTAGCCAAGCCTTTAAGCGCGTGT
ATGGCATATCGCCAAAAGCGTACCGCCAGCAATATCAAAAACAAAACCTTGCTTAG

Upstream 100 bases:

>100_bases
CAGTAGTACTATATTTTTAAATAAAATCGATCCTCATATTAATGCGATTTTTTTATTAAAGTGGCGCGTTATTGAGCAGC
TGTTATTGAGGAAATGGTTA

Downstream 100 bases:

>100_bases
CTGCTACTTCACACATAAATAGTCATCCAAGCTGTGTGATATTTTCTAAACTGAGTCAGTGATATAATAGCGTCTATCTG
ATAATAATTTTTCATCATTA

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MDALSVLLQNVHLFDTKYYRLNGTGNWSYSLTRQDTIVFYLVMSGSFCIDMGSGLREAHAGDIIMIPSAHNHVSYALNHH
GESAKPLDELLTNCTEQVLDIQGDGDAQSSLILIECKYDKEMIRPLLSVLPTILPEINDESDGRFEVIDVEIRLLTLEAE
HERMGKTAIINHWASIMMIECLRVYIESLPEATDSWLKAMKDPFLTKALVAMHENPSQNWSIHELAEVAGMSRSSFAQRF
KDVVGMPPLTYLIDYRLRLAARYLRLQQNSISRISELVGYASDSTFSQAFKRVYGISPKAYRQQYQKQNLA

Sequences:

>Translated_311_residues
MDALSVLLQNVHLFDTKYYRLNGTGNWSYSLTRQDTIVFYLVMSGSFCIDMGSGLREAHAGDIIMIPSAHNHVSYALNHH
GESAKPLDELLTNCTEQVLDIQGDGDAQSSLILIECKYDKEMIRPLLSVLPTILPEINDESDGRFEVIDVEIRLLTLEAE
HERMGKTAIINHWASIMMIECLRVYIESLPEATDSWLKAMKDPFLTKALVAMHENPSQNWSIHELAEVAGMSRSSFAQRF
KDVVGMPPLTYLIDYRLRLAARYLRLQQNSISRISELVGYASDSTFSQAFKRVYGISPKAYRQQYQKQNLA
>Mature_311_residues
MDALSVLLQNVHLFDTKYYRLNGTGNWSYSLTRQDTIVFYLVMSGSFCIDMGSGLREAHAGDIIMIPSAHNHVSYALNHH
GESAKPLDELLTNCTEQVLDIQGDGDAQSSLILIECKYDKEMIRPLLSVLPTILPEINDESDGRFEVIDVEIRLLTLEAE
HERMGKTAIINHWASIMMIECLRVYIESLPEATDSWLKAMKDPFLTKALVAMHENPSQNWSIHELAEVAGMSRSSFAQRF
KDVVGMPPLTYLIDYRLRLAARYLRLQQNSISRISELVGYASDSTFSQAFKRVYGISPKAYRQQYQKQNLA

Specific function: This protein activates the expression of the alkane 1- monooxygenase AlkM [H]

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786496, Length=111, Percent_Identity=39.6396396396396, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI87082197, Length=95, Percent_Identity=35.7894736842105, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 35315; Mature: 35315

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDALSVLLQNVHLFDTKYYRLNGTGNWSYSLTRQDTIVFYLVMSGSFCIDMGSGLREAHA
CCHHHHHHHHHHHCCCEEEEECCCCCEEEEEECCCEEEEEEEECCCEEEECCCCHHHHCC
GDIIMIPSAHNHVSYALNHHGESAKPLDELLTNCTEQVLDIQGDGDAQSSLILIECKYDK
CCEEEECCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCH
EMIRPLLSVLPTILPEINDESDGRFEVIDVEIRLLTLEAEHERMGKTAIINHWASIMMIE
HHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEEEEECHHHHHCCHHHHHHHHHHHHHH
CLRVYIESLPEATDSWLKAMKDPFLTKALVAMHENPSQNWSIHELAEVAGMSRSSFAQRF
HHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHH
KDVVGMPPLTYLIDYRLRLAARYLRLQQNSISRISELVGYASDSTFSQAFKRVYGISPKA
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHH
YRQQYQKQNLA
HHHHHHHHCCC
>Mature Secondary Structure
MDALSVLLQNVHLFDTKYYRLNGTGNWSYSLTRQDTIVFYLVMSGSFCIDMGSGLREAHA
CCHHHHHHHHHHHCCCEEEEECCCCCEEEEEECCCEEEEEEEECCCEEEECCCCHHHHCC
GDIIMIPSAHNHVSYALNHHGESAKPLDELLTNCTEQVLDIQGDGDAQSSLILIECKYDK
CCEEEECCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCH
EMIRPLLSVLPTILPEINDESDGRFEVIDVEIRLLTLEAEHERMGKTAIINHWASIMMIE
HHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEEEEECHHHHHCCHHHHHHHHHHHHHH
CLRVYIESLPEATDSWLKAMKDPFLTKALVAMHENPSQNWSIHELAEVAGMSRSSFAQRF
HHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHH
KDVVGMPPLTYLIDYRLRLAARYLRLQQNSISRISELVGYASDSTFSQAFKRVYGISPKA
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHH
YRQQYQKQNLA
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9546151 [H]