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The map label for this gene is mcrB [H]

Identifier: 93005083

GI number: 93005083

Start: 296467

End: 297963

Strand: Direct

Name: mcrB [H]

Synonym: Pcryo_0252

Alternate gene names: 93005083

Gene position: 296467-297963 (Clockwise)

Preceding gene: 93005080

Following gene: 93005084

Centisome position: 9.69

GC content: 42.89

Gene sequence:

>1497_bases
ATGTTAGCGCTTTTTCATACTTTTTTGTTGGATAATGTTATGCCTGATATGTGTGATGATAAGCCGACCAATATTATTTA
TACGGGCGTCGCGGGTACTGGTAAAACCTACCGATTGCTCCAAATTTCTAAAGAATATACGGACTATCTGCCTAAATCCA
ATAATGAGGATTTACTCAAGCAGTTATTGCAAGAATTGAACTGGCGTGATGTGGTGTGTTTAATATTTTTAGATTTTCAG
TCGCAAAAACAGGACTTAGTAAAAGTACCTGAAATCGTTAATCATGAGTTTTTTATCGCCAAAGCATTACAAAACGCACG
TGAGAAAAATTTAAGTAATACAGCGTGGTCTGTACTGCAAATGCACAGTCCAACCAGCTCACAGACGGTTGCGTATAAAA
ATAGAGCCAGCCAAGCGTATTTTGATAAAGACCCTAGCGGTGCGTGGTATTTACTGCCTGACAGCCTGATGCTATTAAGC
GAATTGTCAGAGCAATTGTCAGAATTTCAGCAGGCGCAACGTGATAATAGTGCCAGCCAAACTCAACGCTTTAGCCAACA
GCGCTTTAGTATGGTCAGTTTCCATCAGGCATATGGCTATGAAGAGTTTGTCGAGGGCATTCGCCCGGTAATGGCAGCTT
CTAATCAGTCGAATAACAGCTCATCTCAAATGAGCTATGCCATTACTGATGGCGCCTTTTTAAAGCTCTGTCAGCGCGCG
GCAAATGATCCTCAGCAGCGTTATGCGATGCTGATTGACGAGATTAATCGTGCCAACGTCTCACGCGTTTTTGGTGAATT
GCTCAGCCTTATTGAACCTGATAAACGAGCAGGTACGCCCAATGCGATGACAGTAAGTTTGGCATACTCTGGGCGAGCGT
TTAGCGTGCCTGCCAATGTTGATATCTATGCGACCATGAATACCCAAGATCATTCGTTAGCGCCACTAGATATGGCACTG
CGTCGTCGTTTTCGCTTTATTAACTGTCCGCCGCAGCCTGAGCTATTACCGATTATCAACCTCAATAAAGGATTAGAAAT
AGTCGACGAAGCGGAGTGCATCAATTTAGCCAAAGTATTAACGGGTTTAAATCATCGTATTAGCCAAACGTTAGGGATTG
AGGCGCAAATGGGTCATGCGTTTTTATTCTCCGTGCATAGTCTTGAGCAGTTACAAATGGCACTTACTGAGCAAATTATT
CCACAATTAGCCCAAGCAGCTGGCGGGCAAGTGGCTGTCTTGCAGTATATTTTTAGAGATGAACAGCAGCCAATATCGGC
ACAATTTATTCATGATAGTCAAACGTCAATACATAACGGTATGTCCACTCAAATGGCTAGCGAGAATGGTATAGCTTCTC
AGAACTCCATGTTTGCAGGACAGCAATCCTATTTTACTCAATCTATAAATGCCGGCAGTTATGCAATTAATACTGACCTT
ATTGCCAATACTGGCAAGTTCACCCAGCTTGCCGTCTATCAGCGCTTATATCCATGA

Upstream 100 bases:

>100_bases
TGTACTGCTGCGCTTACAAAATAACAGTGACGTAACGGCATCTTAGGCGTTAGTAATTTAAGCACTGATACCCTGTAGAG
CGAGTAACTATAATGATAAA

Downstream 100 bases:

>100_bases
GGATGTTAATCGATGATAAATACGCATAAGCACACTCAAAGCTCTGAAATGAATATCATGACGGTATTTGAACATCAGCG
TTTAATAGCGTCTAATTTCT

Product: ATPase

Products: NA

Alternate protein names: EcoKMcrBC [H]

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MLALFHTFLLDNVMPDMCDDKPTNIIYTGVAGTGKTYRLLQISKEYTDYLPKSNNEDLLKQLLQELNWRDVVCLIFLDFQ
SQKQDLVKVPEIVNHEFFIAKALQNAREKNLSNTAWSVLQMHSPTSSQTVAYKNRASQAYFDKDPSGAWYLLPDSLMLLS
ELSEQLSEFQQAQRDNSASQTQRFSQQRFSMVSFHQAYGYEEFVEGIRPVMAASNQSNNSSSQMSYAITDGAFLKLCQRA
ANDPQQRYAMLIDEINRANVSRVFGELLSLIEPDKRAGTPNAMTVSLAYSGRAFSVPANVDIYATMNTQDHSLAPLDMAL
RRRFRFINCPPQPELLPIINLNKGLEIVDEAECINLAKVLTGLNHRISQTLGIEAQMGHAFLFSVHSLEQLQMALTEQII
PQLAQAAGGQVAVLQYIFRDEQQPISAQFIHDSQTSIHNGMSTQMASENGIASQNSMFAGQQSYFTQSINAGSYAINTDL
IANTGKFTQLAVYQRLYP

Sequences:

>Translated_498_residues
MLALFHTFLLDNVMPDMCDDKPTNIIYTGVAGTGKTYRLLQISKEYTDYLPKSNNEDLLKQLLQELNWRDVVCLIFLDFQ
SQKQDLVKVPEIVNHEFFIAKALQNAREKNLSNTAWSVLQMHSPTSSQTVAYKNRASQAYFDKDPSGAWYLLPDSLMLLS
ELSEQLSEFQQAQRDNSASQTQRFSQQRFSMVSFHQAYGYEEFVEGIRPVMAASNQSNNSSSQMSYAITDGAFLKLCQRA
ANDPQQRYAMLIDEINRANVSRVFGELLSLIEPDKRAGTPNAMTVSLAYSGRAFSVPANVDIYATMNTQDHSLAPLDMAL
RRRFRFINCPPQPELLPIINLNKGLEIVDEAECINLAKVLTGLNHRISQTLGIEAQMGHAFLFSVHSLEQLQMALTEQII
PQLAQAAGGQVAVLQYIFRDEQQPISAQFIHDSQTSIHNGMSTQMASENGIASQNSMFAGQQSYFTQSINAGSYAINTDL
IANTGKFTQLAVYQRLYP
>Mature_498_residues
MLALFHTFLLDNVMPDMCDDKPTNIIYTGVAGTGKTYRLLQISKEYTDYLPKSNNEDLLKQLLQELNWRDVVCLIFLDFQ
SQKQDLVKVPEIVNHEFFIAKALQNAREKNLSNTAWSVLQMHSPTSSQTVAYKNRASQAYFDKDPSGAWYLLPDSLMLLS
ELSEQLSEFQQAQRDNSASQTQRFSQQRFSMVSFHQAYGYEEFVEGIRPVMAASNQSNNSSSQMSYAITDGAFLKLCQRA
ANDPQQRYAMLIDEINRANVSRVFGELLSLIEPDKRAGTPNAMTVSLAYSGRAFSVPANVDIYATMNTQDHSLAPLDMAL
RRRFRFINCPPQPELLPIINLNKGLEIVDEAECINLAKVLTGLNHRISQTLGIEAQMGHAFLFSVHSLEQLQMALTEQII
PQLAQAAGGQVAVLQYIFRDEQQPISAQFIHDSQTSIHNGMSTQMASENGIASQNSMFAGQQSYFTQSINAGSYAINTDL
IANTGKFTQLAVYQRLYP

Specific function: Recognizes N4- and C5-methylcytosine (and 5-hydroxy- methylcytosines) produced by a broad range of DNA methylases and appears to act against 5-methylcytosine preceded by a purine residue. Binds to DNA containing methylated cytosines; also binds to GTP. Is

COG id: COG1401

COG function: function code V; GTPase subunit of restriction endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082429, Length=205, Percent_Identity=39.5121951219512, Blast_Score=132, Evalue=6e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011704
- InterPro:   IPR021961
- InterPro:   IPR004491 [H]

Pfam domain/function: PF07728 AAA_5; PF12102 DUF3578 [H]

EC number: 3.1.21.- [C]

Molecular weight: Translated: 55901; Mature: 55901

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLALFHTFLLDNVMPDMCDDKPTNIIYTGVAGTGKTYRLLQISKEYTDYLPKSNNEDLLK
CHHHHHHHHHHHCCHHHCCCCCCCEEEECCCCCCCEEEEEEECHHHHHCCCCCCCHHHHH
QLLQELNWRDVVCLIFLDFQSQKQDLVKVPEIVNHEFFIAKALQNAREKNLSNTAWSVLQ
HHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHH
MHSPTSSQTVAYKNRASQAYFDKDPSGAWYLLPDSLMLLSELSEQLSEFQQAQRDNSASQ
CCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHH
TQRFSQQRFSMVSFHQAYGYEEFVEGIRPVMAASNQSNNSSSQMSYAITDGAFLKLCQRA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHH
ANDPQQRYAMLIDEINRANVSRVFGELLSLIEPDKRAGTPNAMTVSLAYSGRAFSVPANV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCEEEECCCCE
DIYATMNTQDHSLAPLDMALRRRFRFINCPPQPELLPIINLNKGLEIVDEAECINLAKVL
EEEEEECCCCCCCCHHHHHHHHHHEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHH
TGLNHRISQTLGIEAQMGHAFLFSVHSLEQLQMALTEQIIPQLAQAAGGQVAVLQYIFRD
HHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHC
EQQPISAQFIHDSQTSIHNGMSTQMASENGIASQNSMFAGQQSYFTQSINAGSYAINTDL
CCCCCCEEEECCCHHHHHCCCHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCEEEECCC
IANTGKFTQLAVYQRLYP
EECCCCHHHHHHHHHCCC
>Mature Secondary Structure
MLALFHTFLLDNVMPDMCDDKPTNIIYTGVAGTGKTYRLLQISKEYTDYLPKSNNEDLLK
CHHHHHHHHHHHCCHHHCCCCCCCEEEECCCCCCCEEEEEEECHHHHHCCCCCCCHHHHH
QLLQELNWRDVVCLIFLDFQSQKQDLVKVPEIVNHEFFIAKALQNAREKNLSNTAWSVLQ
HHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHH
MHSPTSSQTVAYKNRASQAYFDKDPSGAWYLLPDSLMLLSELSEQLSEFQQAQRDNSASQ
CCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHH
TQRFSQQRFSMVSFHQAYGYEEFVEGIRPVMAASNQSNNSSSQMSYAITDGAFLKLCQRA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHH
ANDPQQRYAMLIDEINRANVSRVFGELLSLIEPDKRAGTPNAMTVSLAYSGRAFSVPANV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCEEEECCCCE
DIYATMNTQDHSLAPLDMALRRRFRFINCPPQPELLPIINLNKGLEIVDEAECINLAKVL
EEEEEECCCCCCCCHHHHHHHHHHEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHH
TGLNHRISQTLGIEAQMGHAFLFSVHSLEQLQMALTEQIIPQLAQAAGGQVAVLQYIFRD
HHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHC
EQQPISAQFIHDSQTSIHNGMSTQMASENGIASQNSMFAGQQSYFTQSINAGSYAINTDL
CCCCCCEEEECCCHHHHHCCCHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCEEEECCC
IANTGKFTQLAVYQRLYP
EECCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds; Endodeoxyribonucleases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2203735; 2649480; 7610040; 9278503; 1312983; 7781618 [H]