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The map label for this gene is metR [H]

Identifier: 93005067

GI number: 93005067

Start: 278400

End: 279290

Strand: Reverse

Name: metR [H]

Synonym: Pcryo_0236

Alternate gene names: 93005067

Gene position: 279290-278400 (Counterclockwise)

Preceding gene: 93005068

Following gene: 93005062

Centisome position: 9.13

GC content: 47.14

Gene sequence:

>891_bases
ATGCTAGAGCTTCGTCATCTTAATACGCTAACTGCGCTGCGAGCACATGGCTCATTGGCTGCTGCCGCCGATGAGCTGCA
TGTCACCGCCTCTGCGGTCTCCCATCAGTTAAAAGAGCTAGAGAATTATTACGATATTAGTTTGGTCAATAGGCGCACAC
GTCCGCTAACTTTTACGCCAGCTGGCAAAACGGTATTGGCACTCGCTGACAGCATTTTACCTCAGGTCACGCGCACCAAG
TCAAACTTAAAACGCTTAGCCCATGGTCAAGCAGGCAGATTGCGCTTGGCGTCTGAATGTCATAGCTGCTTTGATTGGCT
GATGCCGATTCTCAATCATTATCGCCGTGAGTGGTCAGATGTGGAGCTAGATTTTGCTACTGGTTTTGAGCCCGAGCCGC
ATCATTTATTGATGGAAGGTGATATTGATTTATTGATTACTACAAGTAATTTACCGATAGAAGGTATCAGCTATCAGCCA
TTATTTGAGTATGAAAGTCGTTTGGTACTGTCGCCGACTCATGACTTGGCTGAGCAACAATTTATTCATGCCAAAGACTT
AGTCGATGAAACCTTGATTGCTTATCCAGTGGAAGCTAAGCGCCTTGATATTATTGCCAATTTTATGACCCCAGAGCAGG
TCAGCTTTAAAAGTATGCGTACCACCGAGCTAACCGCTATGCTTATTCAGTTGGTTGCTAGTGAACGCGGGGTGGCAGCA
CTCCCCGACTGGGTGGTGGCTGAATATGAGAGAAAAGGTTGGGTAGTTTCACGTCCATTAGGTAGCGGCGTCTACTGTCA
GCTGTATGCGGCAACGCGCACCTCGAGCCAAGAGATTGCCTATATGCAAGGGTTTGCGAGTTTATTAGAAGGTATCGTAA
AACCTGTCTAG

Upstream 100 bases:

>100_bases
ATATTAGATAAAAAGTTTTTAACAATTTTTCAGCCTTTATTTTTAGGCCTTTATAAGTCGCAACTTTTTATTTCTTTAAC
TTCAGTGGTTAGGTCATTTT

Downstream 100 bases:

>100_bases
AAGTAATTATCACTAAAAAAAGAGCCGTATTAAAAAATTAATATGGCTCTTTTTTATTGCTGCATTTTTATTATTATAAA
ACCAACTTAAAGAAATAAAA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MLELRHLNTLTALRAHGSLAAAADELHVTASAVSHQLKELENYYDISLVNRRTRPLTFTPAGKTVLALADSILPQVTRTK
SNLKRLAHGQAGRLRLASECHSCFDWLMPILNHYRREWSDVELDFATGFEPEPHHLLMEGDIDLLITTSNLPIEGISYQP
LFEYESRLVLSPTHDLAEQQFIHAKDLVDETLIAYPVEAKRLDIIANFMTPEQVSFKSMRTTELTAMLIQLVASERGVAA
LPDWVVAEYERKGWVVSRPLGSGVYCQLYAATRTSSQEIAYMQGFASLLEGIVKPV

Sequences:

>Translated_296_residues
MLELRHLNTLTALRAHGSLAAAADELHVTASAVSHQLKELENYYDISLVNRRTRPLTFTPAGKTVLALADSILPQVTRTK
SNLKRLAHGQAGRLRLASECHSCFDWLMPILNHYRREWSDVELDFATGFEPEPHHLLMEGDIDLLITTSNLPIEGISYQP
LFEYESRLVLSPTHDLAEQQFIHAKDLVDETLIAYPVEAKRLDIIANFMTPEQVSFKSMRTTELTAMLIQLVASERGVAA
LPDWVVAEYERKGWVVSRPLGSGVYCQLYAATRTSSQEIAYMQGFASLLEGIVKPV
>Mature_296_residues
MLELRHLNTLTALRAHGSLAAAADELHVTASAVSHQLKELENYYDISLVNRRTRPLTFTPAGKTVLALADSILPQVTRTK
SNLKRLAHGQAGRLRLASECHSCFDWLMPILNHYRREWSDVELDFATGFEPEPHHLLMEGDIDLLITTSNLPIEGISYQP
LFEYESRLVLSPTHDLAEQQFIHAKDLVDETLIAYPVEAKRLDIIANFMTPEQVSFKSMRTTELTAMLIQLVASERGVAA
LPDWVVAEYERKGWVVSRPLGSGVYCQLYAATRTSSQEIAYMQGFASLLEGIVKPV

Specific function: Control of the last step in methionine biosynthesis; metR is a positive activator of the metA, metE and metH genes. It is also a negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790262, Length=285, Percent_Identity=45.2631578947368, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI1786401, Length=158, Percent_Identity=28.4810126582279, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787879, Length=246, Percent_Identity=26.8292682926829, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI157672245, Length=210, Percent_Identity=24.7619047619048, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1789173, Length=249, Percent_Identity=25.7028112449799, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1788887, Length=265, Percent_Identity=23.3962264150943, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI2367136, Length=291, Percent_Identity=21.9931271477663, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1787949, Length=192, Percent_Identity=26.5625, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33215; Mature: 33215

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLELRHLNTLTALRAHGSLAAAADELHVTASAVSHQLKELENYYDISLVNRRTRPLTFTP
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCEEECC
AGKTVLALADSILPQVTRTKSNLKRLAHGQAGRLRLASECHSCFDWLMPILNHYRREWSD
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
VELDFATGFEPEPHHLLMEGDIDLLITTSNLPIEGISYQPLFEYESRLVLSPTHDLAEQQ
EEEEECCCCCCCCCEEEEECCEEEEEEECCCCCCCCCCCCHHHCCCEEEECCCHHHHHHH
FIHAKDLVDETLIAYPVEAKRLDIIANFMTPEQVSFKSMRTTELTAMLIQLVASERGVAA
HHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LPDWVVAEYERKGWVVSRPLGSGVYCQLYAATRTSSQEIAYMQGFASLLEGIVKPV
CCCHHHHHHHHCCEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLELRHLNTLTALRAHGSLAAAADELHVTASAVSHQLKELENYYDISLVNRRTRPLTFTP
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCEEECC
AGKTVLALADSILPQVTRTKSNLKRLAHGQAGRLRLASECHSCFDWLMPILNHYRREWSD
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
VELDFATGFEPEPHHLLMEGDIDLLITTSNLPIEGISYQPLFEYESRLVLSPTHDLAEQQ
EEEEECCCCCCCCCEEEEECCEEEEEEECCCCCCCCCCCCHHHCCCEEEECCCHHHHHHH
FIHAKDLVDETLIAYPVEAKRLDIIANFMTPEQVSFKSMRTTELTAMLIQLVASERGVAA
HHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LPDWVVAEYERKGWVVSRPLGSGVYCQLYAATRTSSQEIAYMQGFASLLEGIVKPV
CCCHHHHHHHHCCEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]