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The map label for this gene is rpoH [H]

Identifier: 93005058

GI number: 93005058

Start: 270806

End: 271669

Strand: Reverse

Name: rpoH [H]

Synonym: Pcryo_0227

Alternate gene names: 93005058

Gene position: 271669-270806 (Counterclockwise)

Preceding gene: 93005059

Following gene: 93005057

Centisome position: 8.88

GC content: 45.25

Gene sequence:

>864_bases
ATGCCGACGCATTTGTCCGCACCTGGGGTGAATTTGGGTGCTTATATTAATACCGTGCACCAAATCCCTATCTTGACTCC
GACGCAAGAGCAAGAGCTTGCCCATCGCTATTATGATGAAGGCGATGTAGAAGCGGCGCGTTTACTGGTCATGTCGCATT
TGCGTTTTGTCATTCATATTGCCCGCAGCTATTCAGGCTATGGGTTACCGCAAGCAGATTTGATCCAAGAAGGTAATTTG
GGACTTATGAAGGCGGTAAAGCGTTTTGATCCTAACAAAGGCGTACGTCTAGTATCATTTGCAGTGCATTGGATCAAAGC
AGAGATTCATGAATTTGTCATCCGTAACTGGCGTATTGTAAAAGTTGCTACAACCAAGGCGCATCGTAAACTGTTCTTTA
ACTTACGCAGTTTAAAAAAGACCAACAATCAGTTGACCCTCGAAGAAGCCGATGCGATTGCCAATGACTTAAACGTTACT
CGTAAGCAAGTGCTTGAGATGGAGTCGCGCCTGACCTCTTATGATGCGTCATTTGAGGCGCAATCAAGTGACGATGACGA
TGGACGTTATGCGCCACAGCTATTTTTGGAAGATGGCATTGACCCTGCCGAAATCGTCGAAGAATCTGACTGGGAAGAAA
ATAATTCATCAGCCTTGATTGCTGCAATGGAAACCTTGGATGATCGCTCACGTGATATTGTCGAGCAGCGCTGGTTATCT
GAGCAAAAATCAACCTTGCATGAGTTGGCAGCGGTCTACTCTATCTCTGCTGAGCGCGTACGCCAAATTGAGAAAAATGC
CATGGATAAAATCCGTGAAGCGATGACCATCGACAGTGTGATTTTACCTGATGATATTCAATAA

Upstream 100 bases:

>100_bases
AAACGAAGCGCGCGATGCAGCACTGAAAGCGGCAGCTGAGCGTCCGCCTTATGATGCCAGCGCGCTTGGCGATACCAGTA
CCCGTGATTTGGTACCTGCC

Downstream 100 bases:

>100_bases
GTTGCTTATCGATAAGACAACTGTCTTTGCTTAGTAAGCATTTATTCAACAATACGAGTACAACCATGTTCAATTGGATA
AGTATCGCGGCGATCAATAT

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MPTHLSAPGVNLGAYINTVHQIPILTPTQEQELAHRYYDEGDVEAARLLVMSHLRFVIHIARSYSGYGLPQADLIQEGNL
GLMKAVKRFDPNKGVRLVSFAVHWIKAEIHEFVIRNWRIVKVATTKAHRKLFFNLRSLKKTNNQLTLEEADAIANDLNVT
RKQVLEMESRLTSYDASFEAQSSDDDDGRYAPQLFLEDGIDPAEIVEESDWEENNSSALIAAMETLDDRSRDIVEQRWLS
EQKSTLHELAAVYSISAERVRQIEKNAMDKIREAMTIDSVILPDDIQ

Sequences:

>Translated_287_residues
MPTHLSAPGVNLGAYINTVHQIPILTPTQEQELAHRYYDEGDVEAARLLVMSHLRFVIHIARSYSGYGLPQADLIQEGNL
GLMKAVKRFDPNKGVRLVSFAVHWIKAEIHEFVIRNWRIVKVATTKAHRKLFFNLRSLKKTNNQLTLEEADAIANDLNVT
RKQVLEMESRLTSYDASFEAQSSDDDDGRYAPQLFLEDGIDPAEIVEESDWEENNSSALIAAMETLDDRSRDIVEQRWLS
EQKSTLHELAAVYSISAERVRQIEKNAMDKIREAMTIDSVILPDDIQ
>Mature_286_residues
PTHLSAPGVNLGAYINTVHQIPILTPTQEQELAHRYYDEGDVEAARLLVMSHLRFVIHIARSYSGYGLPQADLIQEGNLG
LMKAVKRFDPNKGVRLVSFAVHWIKAEIHEFVIRNWRIVKVATTKAHRKLFFNLRSLKKTNNQLTLEEADAIANDLNVTR
KQVLEMESRLTSYDASFEAQSSDDDDGRYAPQLFLEDGIDPAEIVEESDWEENNSSALIAAMETLDDRSRDIVEQRWLSE
QKSTLHELAAVYSISAERVRQIEKNAMDKIREAMTIDSVILPDDIQ

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=272, Percent_Identity=57.3529411764706, Blast_Score=300, Evalue=9e-83,
Organism=Escherichia coli, GI1789098, Length=269, Percent_Identity=28.6245353159851, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1789448, Length=235, Percent_Identity=26.3829787234043, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 32674; Mature: 32543

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTHLSAPGVNLGAYINTVHQIPILTPTQEQELAHRYYDEGDVEAARLLVMSHLRFVIHI
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
ARSYSGYGLPQADLIQEGNLGLMKAVKRFDPNKGVRLVSFAVHWIKAEIHEFVIRNWRIV
HHHCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEE
KVATTKAHRKLFFNLRSLKKTNNQLTLEEADAIANDLNVTRKQVLEMESRLTSYDASFEA
EEEHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
QSSDDDDGRYAPQLFLEDGIDPAEIVEESDWEENNSSALIAAMETLDDRSRDIVEQRWLS
CCCCCCCCCHHHHHHHHCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
EQKSTLHELAAVYSISAERVRQIEKNAMDKIREAMTIDSVILPDDIQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCC
>Mature Secondary Structure 
PTHLSAPGVNLGAYINTVHQIPILTPTQEQELAHRYYDEGDVEAARLLVMSHLRFVIHI
CCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
ARSYSGYGLPQADLIQEGNLGLMKAVKRFDPNKGVRLVSFAVHWIKAEIHEFVIRNWRIV
HHHCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEE
KVATTKAHRKLFFNLRSLKKTNNQLTLEEADAIANDLNVTRKQVLEMESRLTSYDASFEA
EEEHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
QSSDDDDGRYAPQLFLEDGIDPAEIVEESDWEENNSSALIAAMETLDDRSRDIVEQRWLS
CCCCCCCCCHHHHHHHHCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
EQKSTLHELAAVYSISAERVRQIEKNAMDKIREAMTIDSVILPDDIQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7728657; 7698670; 10984043 [H]