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The map label for this gene is nemA [H]

Identifier: 93005032

GI number: 93005032

Start: 236631

End: 237734

Strand: Direct

Name: nemA [H]

Synonym: Pcryo_0201

Alternate gene names: 93005032

Gene position: 236631-237734 (Clockwise)

Preceding gene: 93005030

Following gene: 93005034

Centisome position: 7.73

GC content: 49.09

Gene sequence:

>1104_bases
ATGGCACATGATAATTTATTCGAAACCGTCAAAATGGGCACCCAAACCCTAAAAAATCGCATTATTATGGCACCTTTGAC
GCGCTTGCGCTCTGTCGAGCCCGGTGATGTCCCTACAGCGCTTGCTGGCGAGTACTACTCACAGCGTAGCGGTGCTGGGC
TTGTCATTGCTGAAGCGACACAGGTTTCTTTTCAGGCAAAAGGCTATGCGGGCGCGCCTGGTATCCATACCGAAGATCAG
ACGACAGCGTGGAAAACCATTGTCGACAATGTCCATGCAAAAGGCGGCAAGATAGTAGTACAGCTCTGGCACACTGGTCT
GGTAGCGCACGAAAGCGTTCAGCCTGATGGTAAGGCGCCGATCTCTGCCTCCGATGTTCATGTGGGAGTACGTACTTCAC
TGCGCGATAGCAACAACCAAGCCATTCGAGTTGAAGCGACCACACCGCGTCCTGCGACACTGGAAGAGATAGAGCAAGTT
ATTGCTGATTTCGGTCTTGCGACCAAAAACGCTAAAGAGGCGGGTTTTGATGGAGTGGAAATTCATGGTGCACATGGCTA
TCTATTACATCAATTTTGGGCTGAGCATACCAATCAGCGCACTGATGAATATGGCGGTAGCCGTGAGAACCGTGCGCGCT
TAATGCTGGCTGTGATTGATGCTTGTGTCGACGCATGGGATGCGGACCATGTCGGTATTCGTATTTCACCACTTGGTACG
TTTAATAATGTGGAAGCTGGATATAACGAAGATGAAAACATTTGGCTGATTGAGCAAATCAATCAACGCGCTATTATGTA
TTTGCATTTATCTGAGCCTGATTGGGCAGGTGGAACGCCTTATAGCGATGCGTTCCGTCAGCGTGTTCGCGATGCCTTTG
ATCAGACAATCATTGCCGCAGGTGGTTATACAGCGGAAAAGGCTGAAAGAAACATCAAGTCAGGCTATATCGATGCCGTG
GCGTTTGGCCGTGATTATATCGCCAATCCTGATTTGGCTGAGCGTATTCGTGAAGGCGCGCCGCTAAATGAACAACATCC
AGAGAGCTTTTATGGTGGCGGCACAAAAGGCTATACCGATTATCCGTTTTTAAACCAAGCATAA

Upstream 100 bases:

>100_bases
ATAGCGTGCTTAATGCGTTTTAGACGTAATATTAAGTGTGAATTATTCATCATTAACAAATTCTATAACCGTATTTTTAT
AACAGCAAATAAGGCTTTTT

Downstream 100 bases:

>100_bases
TGGTTTGACGGTCAATAAAAAAGCCACCTTGCTATGATTAGCAAGGTGGCTTTTTATGATTTGGTCAATATGATTTAAAC
TATCTCACCGCAGACAAAAC

Product: N-ethylmaleimide reductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MAHDNLFETVKMGTQTLKNRIIMAPLTRLRSVEPGDVPTALAGEYYSQRSGAGLVIAEATQVSFQAKGYAGAPGIHTEDQ
TTAWKTIVDNVHAKGGKIVVQLWHTGLVAHESVQPDGKAPISASDVHVGVRTSLRDSNNQAIRVEATTPRPATLEEIEQV
IADFGLATKNAKEAGFDGVEIHGAHGYLLHQFWAEHTNQRTDEYGGSRENRARLMLAVIDACVDAWDADHVGIRISPLGT
FNNVEAGYNEDENIWLIEQINQRAIMYLHLSEPDWAGGTPYSDAFRQRVRDAFDQTIIAAGGYTAEKAERNIKSGYIDAV
AFGRDYIANPDLAERIREGAPLNEQHPESFYGGGTKGYTDYPFLNQA

Sequences:

>Translated_367_residues
MAHDNLFETVKMGTQTLKNRIIMAPLTRLRSVEPGDVPTALAGEYYSQRSGAGLVIAEATQVSFQAKGYAGAPGIHTEDQ
TTAWKTIVDNVHAKGGKIVVQLWHTGLVAHESVQPDGKAPISASDVHVGVRTSLRDSNNQAIRVEATTPRPATLEEIEQV
IADFGLATKNAKEAGFDGVEIHGAHGYLLHQFWAEHTNQRTDEYGGSRENRARLMLAVIDACVDAWDADHVGIRISPLGT
FNNVEAGYNEDENIWLIEQINQRAIMYLHLSEPDWAGGTPYSDAFRQRVRDAFDQTIIAAGGYTAEKAERNIKSGYIDAV
AFGRDYIANPDLAERIREGAPLNEQHPESFYGGGTKGYTDYPFLNQA
>Mature_366_residues
AHDNLFETVKMGTQTLKNRIIMAPLTRLRSVEPGDVPTALAGEYYSQRSGAGLVIAEATQVSFQAKGYAGAPGIHTEDQT
TAWKTIVDNVHAKGGKIVVQLWHTGLVAHESVQPDGKAPISASDVHVGVRTSLRDSNNQAIRVEATTPRPATLEEIEQVI
ADFGLATKNAKEAGFDGVEIHGAHGYLLHQFWAEHTNQRTDEYGGSRENRARLMLAVIDACVDAWDADHVGIRISPLGTF
NNVEAGYNEDENIWLIEQINQRAIMYLHLSEPDWAGGTPYSDAFRQRVRDAFDQTIIAAGGYTAEKAERNIKSGYIDAVA
FGRDYIANPDLAERIREGAPLNEQHPESFYGGGTKGYTDYPFLNQA

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=365, Percent_Identity=60.5479452054795, Blast_Score=466, Evalue=1e-133,
Organism=Escherichia coli, GI1789463, Length=263, Percent_Identity=31.1787072243346, Blast_Score=110, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17565138, Length=358, Percent_Identity=31.2849162011173, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17559802, Length=365, Percent_Identity=30.4109589041096, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI72001454, Length=359, Percent_Identity=30.08356545961, Blast_Score=111, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17559804, Length=270, Percent_Identity=32.2222222222222, Blast_Score=111, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17564188, Length=266, Percent_Identity=30.4511278195489, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17566914, Length=354, Percent_Identity=27.1186440677966, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17540738, Length=272, Percent_Identity=28.3088235294118, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6325086, Length=383, Percent_Identity=37.3368146214099, Blast_Score=224, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6321973, Length=388, Percent_Identity=35.8247422680412, Blast_Score=215, Evalue=8e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 40263; Mature: 40132

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHDNLFETVKMGTQTLKNRIIMAPLTRLRSVEPGDVPTALAGEYYSQRSGAGLVIAEAT
CCCCHHHHHHHHHHHHHHCCEEEHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEEECC
QVSFQAKGYAGAPGIHTEDQTTAWKTIVDNVHAKGGKIVVQLWHTGLVAHESVQPDGKAP
EEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCEEECCCCCCCCCC
ISASDVHVGVRTSLRDSNNQAIRVEATTPRPATLEEIEQVIADFGLATKNAKEAGFDGVE
CCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCHHHCCCCCEE
IHGAHGYLLHQFWAEHTNQRTDEYGGSRENRARLMLAVIDACVDAWDADHVGIRISPLGT
EECCCHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCC
FNNVEAGYNEDENIWLIEQINQRAIMYLHLSEPDWAGGTPYSDAFRQRVRDAFDQTIIAA
CCCCCCCCCCCCCEEEEEECCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHEEEEC
GGYTAEKAERNIKSGYIDAVAFGRDYIANPDLAERIREGAPLNEQHPESFYGGGTKGYTD
CCCHHHHHHHHHHCCCHHHEECCCCCCCCCCHHHHHHCCCCCCCCCCHHHCCCCCCCCCC
YPFLNQA
CCCCCCC
>Mature Secondary Structure 
AHDNLFETVKMGTQTLKNRIIMAPLTRLRSVEPGDVPTALAGEYYSQRSGAGLVIAEAT
CCCHHHHHHHHHHHHHHCCEEEHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEEECC
QVSFQAKGYAGAPGIHTEDQTTAWKTIVDNVHAKGGKIVVQLWHTGLVAHESVQPDGKAP
EEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCEEECCCCCCCCCC
ISASDVHVGVRTSLRDSNNQAIRVEATTPRPATLEEIEQVIADFGLATKNAKEAGFDGVE
CCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCHHHCCCCCEE
IHGAHGYLLHQFWAEHTNQRTDEYGGSRENRARLMLAVIDACVDAWDADHVGIRISPLGT
EECCCHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCC
FNNVEAGYNEDENIWLIEQINQRAIMYLHLSEPDWAGGTPYSDAFRQRVRDAFDQTIIAA
CCCCCCCCCCCCCEEEEEECCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHEEEEC
GGYTAEKAERNIKSGYIDAVAFGRDYIANPDLAERIREGAPLNEQHPESFYGGGTKGYTD
CCCHHHHHHHHHHCCCHHHEECCCCCCCCCCHHHHHHCCCCCCCCCCHHHCCCCCCCCCC
YPFLNQA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]