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The map label for this gene is hemA

Identifier: 93005020

GI number: 93005020

Start: 221757

End: 223151

Strand: Direct

Name: hemA

Synonym: Pcryo_0189

Alternate gene names: 93005020

Gene position: 221757-223151 (Clockwise)

Preceding gene: 93005011

Following gene: 93005021

Centisome position: 7.25

GC content: 48.75

Gene sequence:

>1395_bases
ATGAGATTAGTGGTCATTGGGGTCAATCACAAAACTGCACCAGTCGCTCTACGAGAGCGCTTGGCGCTTGTCGGTGACGA
GGTCAGTATCGCACTTGCGCAGCTACAAGGCTTTAGCGACGGTAGCGTGATAGTCTCCACCTGCAACCGGACAGAGATTT
ATGCATTGGTCCCAGAAAGCATATTATCACCACATACGTTATTGCCAAATTCAGCCTCAGCAGTTGTAGAGACTTCAGTT
ATAGAGTCTAGTATGACGGCTAACAATAGCGCCAACCTTTCATCGACTATCATCAGCGCTCACATCCTAAAAATTAAAAC
TTGGCTTGCAGGTTTTAAGCAATTATCACTGGATGAGATTGACCCTTATTTGTATGTCCATCGTAATACGCATGCCTTAA
CCCATTGGCTACGAGTAGCTGCAGGTCTTGACTCTATGATATTGGGTGAGCCGCAGATACTTGGGCAAATCAAGCGCGCT
GTGCATATGGCGCAAGATCAAAAAGCGCTTAGCAATCAGCTAGGTTGGATTGTTGATCAGGTATTTGCAGCGGCAAAACG
AGTACGTAATGAGACCCAAGTTGGCGCACAAGCCGTATCACTTAGCTATGCTGCTGCCAAACTGGTGACGCAAATCTTTG
ATGATTTGCCAAGTCGTACTTTGCTGGTGGTAGCCGCTGGCGAGATGAACCGCTTGGTAGCGACGCATATCGCAGGTCTT
GGAGTCGGTCGCGTTATTATTTGCAACCGTAATCCTGAACGCGCAGAAGCGTTAGCGGCTGAGCTGCGCAATCCCAATCG
CCGGATCGAAGTCAGAACGCTGCAAGAGCTGCCACAAGTATTGGCAGAAGCCGATATTGTGAGCAGCTGTAGCGGTAGCA
TGGACATATTGATTGATAAAACCATGACGCTGCGGGCACTGAAAAGCCGTCGCTATCAGCCGATGCTGATGATTGACTTG
GCGGTGCCGCGTGATATTGATTCGACTGTCAGCCGGATAGACGACGTTTATCTATATTCTGTCGATGACTTACAACATGT
GATAGCCGGTAATATCGAACAACGTCGGCAAGCAGCAGTAGATGCTGAGCTGTTGGTCAGTCAATTGGTGGTTGAGATGG
ATCGCCGCTTTCAAGTACGCCAAGTAGGCAAGGATATTCAGCAATATCGCACGCGTACTCATGAGCAGGTAAACAAACTA
TTGCAGGCGTCTATCGCAGAGCTGCATGGCGATAGTGCTAACCCTGAAGATATTATGATTGAGCTAACGCGGCGCTTAAC
GCAAAACTTGACCCATGCGCCCTCCAAGCTCATGCGTAAGGCGGCACGCGAAGGTGACAATGAATTACTCGATTTTGTGG
TATCAGGTCTACAAGACGCCCATCGGAATCACTGA

Upstream 100 bases:

>100_bases
CTTGTCAAAAGCTGTCTTTGAAAAACGGGATTTTAATTTTTTAAAAACCGCTAAAGGTGTTTGGTTGGCGAGTGAATATC
ATATTTATGGTCTGTCAATA

Downstream 100 bases:

>100_bases
TAGTTGCCTTTTATCATCAAAAATAAGAAATTGTATCAGTATGGTTTTAACGCAAACATAGCATTGTCAACCTGCATTAT
CAGTGCTAATATCGAGCTGT

Product: glutamyl-tRNA reductase

Products: NA

Alternate protein names: GluTR

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MRLVVIGVNHKTAPVALRERLALVGDEVSIALAQLQGFSDGSVIVSTCNRTEIYALVPESILSPHTLLPNSASAVVETSV
IESSMTANNSANLSSTIISAHILKIKTWLAGFKQLSLDEIDPYLYVHRNTHALTHWLRVAAGLDSMILGEPQILGQIKRA
VHMAQDQKALSNQLGWIVDQVFAAAKRVRNETQVGAQAVSLSYAAAKLVTQIFDDLPSRTLLVVAAGEMNRLVATHIAGL
GVGRVIICNRNPERAEALAAELRNPNRRIEVRTLQELPQVLAEADIVSSCSGSMDILIDKTMTLRALKSRRYQPMLMIDL
AVPRDIDSTVSRIDDVYLYSVDDLQHVIAGNIEQRRQAAVDAELLVSQLVVEMDRRFQVRQVGKDIQQYRTRTHEQVNKL
LQASIAELHGDSANPEDIMIELTRRLTQNLTHAPSKLMRKAAREGDNELLDFVVSGLQDAHRNH

Sequences:

>Translated_464_residues
MRLVVIGVNHKTAPVALRERLALVGDEVSIALAQLQGFSDGSVIVSTCNRTEIYALVPESILSPHTLLPNSASAVVETSV
IESSMTANNSANLSSTIISAHILKIKTWLAGFKQLSLDEIDPYLYVHRNTHALTHWLRVAAGLDSMILGEPQILGQIKRA
VHMAQDQKALSNQLGWIVDQVFAAAKRVRNETQVGAQAVSLSYAAAKLVTQIFDDLPSRTLLVVAAGEMNRLVATHIAGL
GVGRVIICNRNPERAEALAAELRNPNRRIEVRTLQELPQVLAEADIVSSCSGSMDILIDKTMTLRALKSRRYQPMLMIDL
AVPRDIDSTVSRIDDVYLYSVDDLQHVIAGNIEQRRQAAVDAELLVSQLVVEMDRRFQVRQVGKDIQQYRTRTHEQVNKL
LQASIAELHGDSANPEDIMIELTRRLTQNLTHAPSKLMRKAAREGDNELLDFVVSGLQDAHRNH
>Mature_464_residues
MRLVVIGVNHKTAPVALRERLALVGDEVSIALAQLQGFSDGSVIVSTCNRTEIYALVPESILSPHTLLPNSASAVVETSV
IESSMTANNSANLSSTIISAHILKIKTWLAGFKQLSLDEIDPYLYVHRNTHALTHWLRVAAGLDSMILGEPQILGQIKRA
VHMAQDQKALSNQLGWIVDQVFAAAKRVRNETQVGAQAVSLSYAAAKLVTQIFDDLPSRTLLVVAAGEMNRLVATHIAGL
GVGRVIICNRNPERAEALAAELRNPNRRIEVRTLQELPQVLAEADIVSSCSGSMDILIDKTMTLRALKSRRYQPMLMIDL
AVPRDIDSTVSRIDDVYLYSVDDLQHVIAGNIEQRRQAAVDAELLVSQLVVEMDRRFQVRQVGKDIQQYRTRTHEQVNKL
LQASIAELHGDSANPEDIMIELTRRLTQNLTHAPSKLMRKAAREGDNELLDFVVSGLQDAHRNH

Specific function: Catalyzes the NADPH-dependent reduction of glutamyl- tRNA(Glu) to glutamate 1-semialdehyde (GSA)

COG id: COG0373

COG function: function code H; Glutamyl-tRNA reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamyl-tRNA reductase family

Homologues:

Organism=Escherichia coli, GI1787461, Length=459, Percent_Identity=39.2156862745098, Blast_Score=290, Evalue=2e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM1_PSYCK (Q1QED0)

Other databases:

- EMBL:   CP000323
- RefSeq:   YP_579457.1
- ProteinModelPortal:   Q1QED0
- SMR:   Q1QED0
- STRING:   Q1QED0
- GeneID:   4035669
- GenomeReviews:   CP000323_GR
- KEGG:   pcr:Pcryo_0189
- NMPDR:   fig|335284.3.peg.798
- eggNOG:   COG0373
- HOGENOM:   HBG732626
- OMA:   EDIMIEL
- PhylomeDB:   Q1QED0
- ProtClustDB:   PRK00045
- BioCyc:   PCRY335284:PCRYO_0189-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00087
- InterPro:   IPR000343
- InterPro:   IPR015896
- InterPro:   IPR015895
- InterPro:   IPR016040
- InterPro:   IPR003462
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR13812
- PIRSF:   PIRSF000445
- TIGRFAMs:   TIGR01035

Pfam domain/function: PF00745 GlutR_dimer; PF05201 GlutR_N; PF01488 Shikimate_DH; SSF69075 4pyrrol_synth_GluRdtase_C; SSF69742 GlutR

EC number: =1.2.1.70

Molecular weight: Translated: 51285; Mature: 51285

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: PS00747 GLUTR

Important sites: ACT_SITE 48-48 BINDING 145-145 BINDING 156-156

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLVVIGVNHKTAPVALRERLALVGDEVSIALAQLQGFSDGSVIVSTCNRTEIYALVPES
CEEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEEEECCCCEEEEECCHH
ILSPHTLLPNSASAVVETSVIESSMTANNSANLSSTIISAHILKIKTWLAGFKQLSLDEI
HCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHC
DPYLYVHRNTHALTHWLRVAAGLDSMILGEPQILGQIKRAVHMAQDQKALSNQLGWIVDQ
CCEEEEECCHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VFAAAKRVRNETQVGAQAVSLSYAAAKLVTQIFDDLPSRTLLVVAAGEMNRLVATHIAGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHC
GVGRVIICNRNPERAEALAAELRNPNRRIEVRTLQELPQVLAEADIVSSCSGSMDILIDK
CCCEEEEECCCHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
TMTLRALKSRRYQPMLMIDLAVPRDIDSTVSRIDDVYLYSVDDLQHVIAGNIEQRRQAAV
HHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHEEEEEHHHHHHHHHCCHHHHHHHHH
DAELLVSQLVVEMDRRFQVRQVGKDIQQYRTRTHEQVNKLLQASIAELHGDSANPEDIMI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
ELTRRLTQNLTHAPSKLMRKAAREGDNELLDFVVSGLQDAHRNH
HHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRLVVIGVNHKTAPVALRERLALVGDEVSIALAQLQGFSDGSVIVSTCNRTEIYALVPES
CEEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEEEECCCCEEEEECCHH
ILSPHTLLPNSASAVVETSVIESSMTANNSANLSSTIISAHILKIKTWLAGFKQLSLDEI
HCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHC
DPYLYVHRNTHALTHWLRVAAGLDSMILGEPQILGQIKRAVHMAQDQKALSNQLGWIVDQ
CCEEEEECCHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VFAAAKRVRNETQVGAQAVSLSYAAAKLVTQIFDDLPSRTLLVVAAGEMNRLVATHIAGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHC
GVGRVIICNRNPERAEALAAELRNPNRRIEVRTLQELPQVLAEADIVSSCSGSMDILIDK
CCCEEEEECCCHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
TMTLRALKSRRYQPMLMIDLAVPRDIDSTVSRIDDVYLYSVDDLQHVIAGNIEQRRQAAV
HHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHEEEEEHHHHHHHHHCCHHHHHHHHH
DAELLVSQLVVEMDRRFQVRQVGKDIQQYRTRTHEQVNKLLQASIAELHGDSANPEDIMI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
ELTRRLTQNLTHAPSKLMRKAAREGDNELLDFVVSGLQDAHRNH
HHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA