Click here to switch to the map view.

The map label for this gene is truB

Identifier: 93004907

GI number: 93004907

Start: 87911

End: 88990

Strand: Direct

Name: truB

Synonym: Pcryo_0076

Alternate gene names: 93004907

Gene position: 87911-88990 (Clockwise)

Preceding gene: 93004906

Following gene: 93004908

Centisome position: 2.87

GC content: 41.85

Gene sequence:

>1080_bases
ATGTCTATCGCATCTACGCAAGCGGATAAAAAGTCTAGTAATCACCCTAACAAGATTAAAGTTTCTGGTGTTATTTTAGT
CGATAAGCCTCAAGGTATGACTTCTCAGCAAGTGGTTTCAAAGGTAAAATATTTATTTAAATCGCCTAACCATGACAGCA
AGAAAGCAGGTCATACCGGCACACTTGATCCAATGGCAACAGGATTATTGCCTATCTGTTTGGGTGAAGCGACCAAGTTC
AGTCACTATCAGCTTGATGCGGATAAATCTTATCAAGCAACCATTCTACTTGGTAGTCAAACTGATACCGGTGATGCCGA
TGGGCAAGTCACTGCTGAGGCACCGATTCCAGCGTTTGATGATGCACTGTTAGACAAAGTCGCTCAGCAGTTTTTAGGTG
CCCAGCAGCAAATTCCACCGATGTATTCTGCCTTAAAAAAAGATGGCAAAAAACTCTATGAATATGCTCGTGCTGGTATA
GAAGTAGACCGCCCGCCAAGAGACATTGTCCTTAAAGCCATTGAGTTAAAAGCAATCGATGAGCAGCAAATCCAGTTAAC
AGTAACTTGTTCAAAAGGCACTTATGTGCGTGTATTGGCAGAAGATATTGCTAAAGCGATGGGTACACTTGGACATTTAA
CGGCATTGCGCCGTCTACAAGTGGGTGATTTTAAAATCGATGAGACAATTGCGCTTGCAGATTTAGAAGCATTGCCATTA
GAACAACGTCAGACATATCTATTGCCAGTAGATGCCTGCATTGATATTAGTGCTGAGCTGTCACTATCGTCAGAGCAGTG
CGAGCGCGTACAAATGGGTCAACGCTTGAATGTTATCGATCAGTTGACTGATGATGTGCAAAGTTATATCACAACAGCTA
TCGATCAGCATTTAGCTGCTAACAATAACAGCGCTGCTGATAGTCAAAATATAGAAGATACTAACGATGATAACGAGCAG
CAATTGGTACATGAAATACCGATAGATATTCGTCTTATTGATGAGCAGGGTGCGTTTATTGGTTTAGGGGCAGTGAGCTT
AAATGGTCGATTGCAACCTAAAAAGCTGATTCAGTTGTAA

Upstream 100 bases:

>100_bases
GGTAATTACATGATGGATTTAATCAGTAAAGCGGTTATTAAAACTGAAGAAAATGAGTCTGACGAGCAAGAAAACGAAGA
GTAGAAGGGTAAAAGTCATT

Downstream 100 bases:

>100_bases
TTTCAACATGTAAATCTGGTTAGATTTATAACTTAAAAGCTGCAAAACTTACTAAGAGTTCACACAAATCACATATCGTC
ACATTTTATTGCAGGTTTCA

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MSIASTQADKKSSNHPNKIKVSGVILVDKPQGMTSQQVVSKVKYLFKSPNHDSKKAGHTGTLDPMATGLLPICLGEATKF
SHYQLDADKSYQATILLGSQTDTGDADGQVTAEAPIPAFDDALLDKVAQQFLGAQQQIPPMYSALKKDGKKLYEYARAGI
EVDRPPRDIVLKAIELKAIDEQQIQLTVTCSKGTYVRVLAEDIAKAMGTLGHLTALRRLQVGDFKIDETIALADLEALPL
EQRQTYLLPVDACIDISAELSLSSEQCERVQMGQRLNVIDQLTDDVQSYITTAIDQHLAANNNSAADSQNIEDTNDDNEQ
QLVHEIPIDIRLIDEQGAFIGLGAVSLNGRLQPKKLIQL

Sequences:

>Translated_359_residues
MSIASTQADKKSSNHPNKIKVSGVILVDKPQGMTSQQVVSKVKYLFKSPNHDSKKAGHTGTLDPMATGLLPICLGEATKF
SHYQLDADKSYQATILLGSQTDTGDADGQVTAEAPIPAFDDALLDKVAQQFLGAQQQIPPMYSALKKDGKKLYEYARAGI
EVDRPPRDIVLKAIELKAIDEQQIQLTVTCSKGTYVRVLAEDIAKAMGTLGHLTALRRLQVGDFKIDETIALADLEALPL
EQRQTYLLPVDACIDISAELSLSSEQCERVQMGQRLNVIDQLTDDVQSYITTAIDQHLAANNNSAADSQNIEDTNDDNEQ
QLVHEIPIDIRLIDEQGAFIGLGAVSLNGRLQPKKLIQL
>Mature_358_residues
SIASTQADKKSSNHPNKIKVSGVILVDKPQGMTSQQVVSKVKYLFKSPNHDSKKAGHTGTLDPMATGLLPICLGEATKFS
HYQLDADKSYQATILLGSQTDTGDADGQVTAEAPIPAFDDALLDKVAQQFLGAQQQIPPMYSALKKDGKKLYEYARAGIE
VDRPPRDIVLKAIELKAIDEQQIQLTVTCSKGTYVRVLAEDIAKAMGTLGHLTALRRLQVGDFKIDETIALADLEALPLE
QRQTYLLPVDACIDISAELSLSSEQCERVQMGQRLNVIDQLTDDVQSYITTAIDQHLAANNNSAADSQNIEDTNDDNEQQ
LVHEIPIDIRLIDEQGAFIGLGAVSLNGRLQPKKLIQL

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=222, Percent_Identity=34.2342342342342, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI215599015, Length=201, Percent_Identity=27.363184079602, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI4503337, Length=201, Percent_Identity=27.363184079602, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI2367200, Length=338, Percent_Identity=37.5739644970414, Blast_Score=207, Evalue=7e-55,
Organism=Caenorhabditis elegans, GI17553978, Length=201, Percent_Identity=26.865671641791, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324037, Length=188, Percent_Identity=31.9148936170213, Blast_Score=95, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323204, Length=215, Percent_Identity=28.8372093023256, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281364189, Length=201, Percent_Identity=27.363184079602, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI281364187, Length=201, Percent_Identity=27.363184079602, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI281364185, Length=201, Percent_Identity=27.363184079602, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI281364183, Length=201, Percent_Identity=27.363184079602, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI62471759, Length=201, Percent_Identity=27.363184079602, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI17975520, Length=201, Percent_Identity=27.363184079602, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_PSYCK (Q1QEP3)

Other databases:

- EMBL:   CP000323
- RefSeq:   YP_579344.1
- ProteinModelPortal:   Q1QEP3
- SMR:   Q1QEP3
- STRING:   Q1QEP3
- GeneID:   4034202
- GenomeReviews:   CP000323_GR
- KEGG:   pcr:Pcryo_0076
- NMPDR:   fig|335284.3.peg.652
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   ARPVQID
- PhylomeDB:   Q1QEP3
- ProtClustDB:   CLSK2516601
- BioCyc:   PCRY335284:PCRYO_0076-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom; SSF88697 PUA-like

EC number: 4.2.1.70

Molecular weight: Translated: 39175; Mature: 39044

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: ACT_SITE 63-63

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIASTQADKKSSNHPNKIKVSGVILVDKPQGMTSQQVVSKVKYLFKSPNHDSKKAGHTG
CCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC
TLDPMATGLLPICLGEATKFSHYQLDADKSYQATILLGSQTDTGDADGQVTAEAPIPAFD
CCCHHHHCCHHHHHCCCCCCCEEEECCCCCEEEEEEEECCCCCCCCCCEEEECCCCCCCH
DALLDKVAQQFLGAQQQIPPMYSALKKDGKKLYEYARAGIEVDRPPRDIVLKAIELKAID
HHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHEECC
EQQIQLTVTCSKGTYVRVLAEDIAKAMGTLGHLTALRRLQVGDFKIDETIALADLEALPL
CCEEEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCEEEEECCHHCCC
EQRQTYLLPVDACIDISAELSLSSEQCERVQMGQRLNVIDQLTDDVQSYITTAIDQHLAA
CCCCEEEEEEHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHC
NNNSAADSQNIEDTNDDNEQQLVHEIPIDIRLIDEQGAFIGLGAVSLNGRLQPKKLIQL
CCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEECCCCEEEEEEEEECCCCCHHHHHCC
>Mature Secondary Structure 
SIASTQADKKSSNHPNKIKVSGVILVDKPQGMTSQQVVSKVKYLFKSPNHDSKKAGHTG
CCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC
TLDPMATGLLPICLGEATKFSHYQLDADKSYQATILLGSQTDTGDADGQVTAEAPIPAFD
CCCHHHHCCHHHHHCCCCCCCEEEECCCCCEEEEEEEECCCCCCCCCCEEEECCCCCCCH
DALLDKVAQQFLGAQQQIPPMYSALKKDGKKLYEYARAGIEVDRPPRDIVLKAIELKAID
HHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHEECC
EQQIQLTVTCSKGTYVRVLAEDIAKAMGTLGHLTALRRLQVGDFKIDETIALADLEALPL
CCEEEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCEEEEECCHHCCC
EQRQTYLLPVDACIDISAELSLSSEQCERVQMGQRLNVIDQLTDDVQSYITTAIDQHLAA
CCCCEEEEEEHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHC
NNNSAADSQNIEDTNDDNEQQLVHEIPIDIRLIDEQGAFIGLGAVSLNGRLQPKKLIQL
CCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEECCCCEEEEEEEEECCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA