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The map label for this gene is nusA [H]

Identifier: 93004904

GI number: 93004904

Start: 82931

End: 84415

Strand: Direct

Name: nusA [H]

Synonym: Pcryo_0073

Alternate gene names: 93004904

Gene position: 82931-84415 (Clockwise)

Preceding gene: 93004903

Following gene: 93004905

Centisome position: 2.71

GC content: 43.23

Gene sequence:

>1485_bases
ATGAGTCGTGAAATCTTAACGGTAGTAGAAACTGTCAGTAACGAGAAGGGCTTAAATCCTGAAGACATCTTTGAAGCAAT
CGAAGATGCTTTGGTCGTCTCGACTAAGAAAAAAGTATATACCGATCAGCCGGAAGTAGAAGTGCGGGTTTCAATTGACC
GTACAACAGGCGATTATGATACTTATCGTTATTGGACAGTGGTTGCGGATGAAGACCATGAAATGCCTGCTTGTCAGCTT
GCGATTACTGACCTTGATCAAGAAGAATGGTCAATCGGTGATATTAAAGAAGAGCAAATTGAGTCGATCGAGTTTGGTCG
TATTGCTGCCACTCAAGCCAAACAAGTTATCATCCAAAAGATTCGTGAAGCTGAGCGTAATTTAGTAGCAGATGCTTTTG
AGCCACGTGTTGGCGAGATGATGTATGGCGAAGTCAAAAAACAAACCCGTGATGGTTACATCATTGACTTAGGTGATAAT
GCTGAGGGTTATCTATCACGTGATCAGATGCTACCACGTGAGCAATTGCGCGCAAAATCGCGTATCAATGCCATCTTATA
TCATGTGAACCGTGAAAATCGCGGCGCTCAGTTGCTTCTATCACGTACTCATCCTGAAATGCTCTCGGCATTGATGCAAA
AAGAAGTGCCTGAGATTGCAGAACAAATTATCGAAATCCGTAACGTCGCGCGCTTACCGGGTACTCGTGCTAAAATAGCG
GTTAAGACCAACGATCATCGTATCGATCCGGTTGGTGCTTGTATTGGTATGCGTGGTACACGTATCCAAGCAGTACAGCA
AGAGCTTGATGGCGAGCGTATTGATGTAGTGGTTTGGTCAGATGATCCTGCCCAATTTATCATCAGCGCTTTAGAGCCTG
CAGACGTAAGCAGTATCATCCTAGATGAAGATACGCAAACGGCTGATATTATCTTTAGTACCAATGATCAGCTAGCACGT
GCGATTGGCTCACAAGGTCAAAACGTACGCTTAGCTTCTGAGCTGACCGGCTATAAGCTAAATATGATGCTAGAAGAAGA
GTATCAGCAGCGTCAAGCAAACGAATCTAAAGCATTCATCGAACTATTCTATGAACGCCTAGAAGTGGATAAAGACCTAG
CACAAGCGCTTGTCGATATTGGTTTCACCAGTATTGAAGAAGTGGCTTATGTACCTGTCGAAACCTTCTACGATATTGAA
GGATTAGATGATGAAGCGATTGATATGATCCAAGAGCGTGCGAAAGAAGTCGTTATTGCAGACGAATTGGTCAAACAACA
GAACATGAAAGAGCCAAGTCAGGAACTACAAGATCTTGAAGGAATGACAGTCAGTTGGGCTTATAAAATGGCTCAAAAAG
ACATCATTACGGTGGATGACTTGGCAGAACAAGCCGTCTTCGACTTAGAAGATATCGAAGACTTAGATACTGAAACCGCA
GGAAAACTCATCATGAAAGCTCGGGAATCTTGGTTCAATGAATAA

Upstream 100 bases:

>100_bases
GCAGTTAGTCTAATAGTTTAGATGGCTGATGGTGAAAATATAGAGTAAGTCAAAAATTATAAAATTTAAGTCAATTAAAA
AATGATAGGACAGGCATAGT

Downstream 100 bases:

>100_bases
GCGGTAACTGCATATCGCTGTCTTTTGGTGATATGCTATCGATAATAAAGTGCTGGTATGAGCAAAATTTATATCAGCCT
TACCCCAATCATTTGTAGCC

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MSREILTVVETVSNEKGLNPEDIFEAIEDALVVSTKKKVYTDQPEVEVRVSIDRTTGDYDTYRYWTVVADEDHEMPACQL
AITDLDQEEWSIGDIKEEQIESIEFGRIAATQAKQVIIQKIREAERNLVADAFEPRVGEMMYGEVKKQTRDGYIIDLGDN
AEGYLSRDQMLPREQLRAKSRINAILYHVNRENRGAQLLLSRTHPEMLSALMQKEVPEIAEQIIEIRNVARLPGTRAKIA
VKTNDHRIDPVGACIGMRGTRIQAVQQELDGERIDVVVWSDDPAQFIISALEPADVSSIILDEDTQTADIIFSTNDQLAR
AIGSQGQNVRLASELTGYKLNMMLEEEYQQRQANESKAFIELFYERLEVDKDLAQALVDIGFTSIEEVAYVPVETFYDIE
GLDDEAIDMIQERAKEVVIADELVKQQNMKEPSQELQDLEGMTVSWAYKMAQKDIITVDDLAEQAVFDLEDIEDLDTETA
GKLIMKARESWFNE

Sequences:

>Translated_494_residues
MSREILTVVETVSNEKGLNPEDIFEAIEDALVVSTKKKVYTDQPEVEVRVSIDRTTGDYDTYRYWTVVADEDHEMPACQL
AITDLDQEEWSIGDIKEEQIESIEFGRIAATQAKQVIIQKIREAERNLVADAFEPRVGEMMYGEVKKQTRDGYIIDLGDN
AEGYLSRDQMLPREQLRAKSRINAILYHVNRENRGAQLLLSRTHPEMLSALMQKEVPEIAEQIIEIRNVARLPGTRAKIA
VKTNDHRIDPVGACIGMRGTRIQAVQQELDGERIDVVVWSDDPAQFIISALEPADVSSIILDEDTQTADIIFSTNDQLAR
AIGSQGQNVRLASELTGYKLNMMLEEEYQQRQANESKAFIELFYERLEVDKDLAQALVDIGFTSIEEVAYVPVETFYDIE
GLDDEAIDMIQERAKEVVIADELVKQQNMKEPSQELQDLEGMTVSWAYKMAQKDIITVDDLAEQAVFDLEDIEDLDTETA
GKLIMKARESWFNE
>Mature_493_residues
SREILTVVETVSNEKGLNPEDIFEAIEDALVVSTKKKVYTDQPEVEVRVSIDRTTGDYDTYRYWTVVADEDHEMPACQLA
ITDLDQEEWSIGDIKEEQIESIEFGRIAATQAKQVIIQKIREAERNLVADAFEPRVGEMMYGEVKKQTRDGYIIDLGDNA
EGYLSRDQMLPREQLRAKSRINAILYHVNRENRGAQLLLSRTHPEMLSALMQKEVPEIAEQIIEIRNVARLPGTRAKIAV
KTNDHRIDPVGACIGMRGTRIQAVQQELDGERIDVVVWSDDPAQFIISALEPADVSSIILDEDTQTADIIFSTNDQLARA
IGSQGQNVRLASELTGYKLNMMLEEEYQQRQANESKAFIELFYERLEVDKDLAQALVDIGFTSIEEVAYVPVETFYDIEG
LDDEAIDMIQERAKEVVIADELVKQQNMKEPSQELQDLEGMTVSWAYKMAQKDIITVDDLAEQAVFDLEDIEDLDTETAG
KLIMKARESWFNE

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=497, Percent_Identity=49.6981891348088, Blast_Score=480, Evalue=1e-136,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR003583
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 56154; Mature: 56023

Theoretical pI: Translated: 4.11; Mature: 4.11

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSREILTVVETVSNEKGLNPEDIFEAIEDALVVSTKKKVYTDQPEVEVRVSIDRTTGDYD
CCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCCEEEEEEEECCCCCCC
TYRYWTVVADEDHEMPACQLAITDLDQEEWSIGDIKEEQIESIEFGRIAATQAKQVIIQK
CEEEEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHH
IREAERNLVADAFEPRVGEMMYGEVKKQTRDGYIIDLGDNAEGYLSRDQMLPREQLRAKS
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHCCCHHHHHHHH
RINAILYHVNRENRGAQLLLSRTHPEMLSALMQKEVPEIAEQIIEIRNVARLPGTRAKIA
HHEEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
VKTNDHRIDPVGACIGMRGTRIQAVQQELDGERIDVVVWSDDPAQFIISALEPADVSSII
EECCCCCCCHHHHHHCCCCHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHCCCCCCCEEE
LDEDTQTADIIFSTNDQLARAIGSQGQNVRLASELTGYKLNMMLEEEYQQRQANESKAFI
ECCCCCCEEEEEECCHHHHHHHCCCCCCEEEHHHCCCEEEEEEEHHHHHHHHCCCHHHHH
ELFYERLEVDKDLAQALVDIGFTSIEEVAYVPVETFYDIEGLDDEAIDMIQERAKEVVIA
HHHHHHHHCCHHHHHHHHHCCCHHHHHHEECCHHHEECCCCCCHHHHHHHHHHHHHHHHH
DELVKQQNMKEPSQELQDLEGMTVSWAYKMAQKDIITVDDLAEQAVFDLEDIEDLDTETA
HHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHCCCEEHHHHHHHHHHCHHHHHHCCHHHH
GKLIMKARESWFNE
HHHHHHHHHHHCCC
>Mature Secondary Structure 
SREILTVVETVSNEKGLNPEDIFEAIEDALVVSTKKKVYTDQPEVEVRVSIDRTTGDYD
CCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCCEEEEEEEECCCCCCC
TYRYWTVVADEDHEMPACQLAITDLDQEEWSIGDIKEEQIESIEFGRIAATQAKQVIIQK
CEEEEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHH
IREAERNLVADAFEPRVGEMMYGEVKKQTRDGYIIDLGDNAEGYLSRDQMLPREQLRAKS
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHCCCHHHHHHHH
RINAILYHVNRENRGAQLLLSRTHPEMLSALMQKEVPEIAEQIIEIRNVARLPGTRAKIA
HHEEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
VKTNDHRIDPVGACIGMRGTRIQAVQQELDGERIDVVVWSDDPAQFIISALEPADVSSII
EECCCCCCCHHHHHHCCCCHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHCCCCCCCEEE
LDEDTQTADIIFSTNDQLARAIGSQGQNVRLASELTGYKLNMMLEEEYQQRQANESKAFI
ECCCCCCEEEEEECCHHHHHHHCCCCCCEEEHHHCCCEEEEEEEHHHHHHHHCCCHHHHH
ELFYERLEVDKDLAQALVDIGFTSIEEVAYVPVETFYDIEGLDDEAIDMIQERAKEVVIA
HHHHHHHHCCHHHHHHHHHCCCHHHHHHEECCHHHEECCCCCCHHHHHHHHHHHHHHHHH
DELVKQQNMKEPSQELQDLEGMTVSWAYKMAQKDIITVDDLAEQAVFDLEDIEDLDTETA
HHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHCCCEEHHHHHHHHHHCHHHHHHCCHHHH
GKLIMKARESWFNE
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12704232 [H]