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The map label for this gene is tpiA [H]

Identifier: 93004901

GI number: 93004901

Start: 80047

End: 80856

Strand: Direct

Name: tpiA [H]

Synonym: Pcryo_0070

Alternate gene names: 93004901

Gene position: 80047-80856 (Clockwise)

Preceding gene: 93004896

Following gene: 93004902

Centisome position: 2.62

GC content: 44.32

Gene sequence:

>810_bases
ATGCAAGCTTGGGTAATTGGAAATTGGAAGCAGAATCCAGCAACGAGCCATGACGTAAACGCACTACTGGACGATTTATG
CGCTGCTATTAGTACAACCAAGCAAATGAGCCACGATAACTCCGCGCGTTGTCAAATAATGGTAGCACCAAGCTTCATAC
ATTTAGCAGCAGTGAATGGTCGCCTAAAAGACACCTCAATACTGTGCGCAGCTCAAGACGTTAGTGGACACAGTGCCAGT
GTTGGTGCTTATACAGGTGACTGTTCAGCGCAGCAGATAGTCGATGCTGGAGCGACTTGGACAATTCTTGGTCACTCAGA
GCGCCGTCAGTATCATCAAGAATCTAACGATACTCTATTGCAAAAAATGATTCATGCGTTGTCACAAGATTTAGGCGTGG
TATTTTGCATTGGTGAAAGCCAAGCACAATATGATACCAAGCAAACGCTTGAAGTCATCGATAATCAGTTAGCAGTTATC
AAAGAATTCATTACACAGCAGCCAGAACTTATTAAGGCATTACCGACTCGTCTCATTATTGCTTATGAGCCAGTATGGGC
AATAGGTACAGGTAAAGTGCCAACGGTAGCAGAAGTCAGCGCCACTCATAAGCATATCAAGCAAATATTGGCAGGATTTG
CCGACTCATTGTCTAATATGACGGTTTTGTATGGCGGTAGTGTCAATGCTGATAATGCTAATAGCTTTGCTGCTGATCCT
ATGATTGATGGCGCTTTAGTTGGCGGTGCTTCATTGAAAGCTGACAGCTTTCTGACGATTGCCACTGCTTTTAGCCAAGC
CAGTATCTAG

Upstream 100 bases:

>100_bases
GATTTGGGGCGCACTATACTATAGCACTTGTGAAATATTTGTTATACTTGCGCGCATATTAGCCATATTGGCAGCCATAT
ATAAGACAAGGTAGAGAAGA

Downstream 100 bases:

>100_bases
TAGATTCTTGATAGATATAATAAAAACGTGAGTCTTTATTGTAATTGTTATTCACAAAATGTCTTTATCAGTTGGCAAAA
GTGGGTTTAACAGTGCCCTT

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MQAWVIGNWKQNPATSHDVNALLDDLCAAISTTKQMSHDNSARCQIMVAPSFIHLAAVNGRLKDTSILCAAQDVSGHSAS
VGAYTGDCSAQQIVDAGATWTILGHSERRQYHQESNDTLLQKMIHALSQDLGVVFCIGESQAQYDTKQTLEVIDNQLAVI
KEFITQQPELIKALPTRLIIAYEPVWAIGTGKVPTVAEVSATHKHIKQILAGFADSLSNMTVLYGGSVNADNANSFAADP
MIDGALVGGASLKADSFLTIATAFSQASI

Sequences:

>Translated_269_residues
MQAWVIGNWKQNPATSHDVNALLDDLCAAISTTKQMSHDNSARCQIMVAPSFIHLAAVNGRLKDTSILCAAQDVSGHSAS
VGAYTGDCSAQQIVDAGATWTILGHSERRQYHQESNDTLLQKMIHALSQDLGVVFCIGESQAQYDTKQTLEVIDNQLAVI
KEFITQQPELIKALPTRLIIAYEPVWAIGTGKVPTVAEVSATHKHIKQILAGFADSLSNMTVLYGGSVNADNANSFAADP
MIDGALVGGASLKADSFLTIATAFSQASI
>Mature_269_residues
MQAWVIGNWKQNPATSHDVNALLDDLCAAISTTKQMSHDNSARCQIMVAPSFIHLAAVNGRLKDTSILCAAQDVSGHSAS
VGAYTGDCSAQQIVDAGATWTILGHSERRQYHQESNDTLLQKMIHALSQDLGVVFCIGESQAQYDTKQTLEVIDNQLAVI
KEFITQQPELIKALPTRLIIAYEPVWAIGTGKVPTVAEVSATHKHIKQILAGFADSLSNMTVLYGGSVNADNANSFAADP
MIDGALVGGASLKADSFLTIATAFSQASI

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family [H]

Homologues:

Organism=Homo sapiens, GI4507645, Length=262, Percent_Identity=35.4961832061069, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI226529917, Length=262, Percent_Identity=35.4961832061069, Blast_Score=143, Evalue=2e-34,
Organism=Escherichia coli, GI1790353, Length=261, Percent_Identity=40.2298850574713, Blast_Score=166, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17536593, Length=265, Percent_Identity=38.4905660377359, Blast_Score=149, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6320255, Length=214, Percent_Identity=37.3831775700935, Blast_Score=141, Evalue=9e-35,
Organism=Drosophila melanogaster, GI28572008, Length=192, Percent_Identity=39.0625, Blast_Score=128, Evalue=3e-30,
Organism=Drosophila melanogaster, GI28572006, Length=192, Percent_Identity=39.0625, Blast_Score=128, Evalue=3e-30,
Organism=Drosophila melanogaster, GI28572004, Length=194, Percent_Identity=38.659793814433, Blast_Score=128, Evalue=4e-30,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861 [H]

Pfam domain/function: PF00121 TIM [H]

EC number: =5.3.1.1 [H]

Molecular weight: Translated: 28698; Mature: 28698

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00171 TIM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAWVIGNWKQNPATSHDVNALLDDLCAAISTTKQMSHDNSARCQIMVAPSFIHLAAVNG
CCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEEECC
RLKDTSILCAAQDVSGHSASVGAYTGDCSAQQIVDAGATWTILGHSERRQYHQESNDTLL
CCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHCCCEEEEECCHHHHHHHHCCCHHHH
QKMIHALSQDLGVVFCIGESQAQYDTKQTLEVIDNQLAVIKEFITQQPELIKALPTRLII
HHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCEEEE
AYEPVWAIGTGKVPTVAEVSATHKHIKQILAGFADSLSNMTVLYGGSVNADNANSFAADP
EECCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCC
MIDGALVGGASLKADSFLTIATAFSQASI
CCCCEEECCCCCCCCCEEEEEEHHHHCCC
>Mature Secondary Structure
MQAWVIGNWKQNPATSHDVNALLDDLCAAISTTKQMSHDNSARCQIMVAPSFIHLAAVNG
CCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEEECC
RLKDTSILCAAQDVSGHSASVGAYTGDCSAQQIVDAGATWTILGHSERRQYHQESNDTLL
CCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHCCCEEEEECCHHHHHHHHCCCHHHH
QKMIHALSQDLGVVFCIGESQAQYDTKQTLEVIDNQLAVIKEFITQQPELIKALPTRLII
HHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCEEEE
AYEPVWAIGTGKVPTVAEVSATHKHIKQILAGFADSLSNMTVLYGGSVNADNANSFAADP
EECCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCC
MIDGALVGGASLKADSFLTIATAFSQASI
CCCCEEECCCCCCCCCEEEEEEHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8421318 [H]