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The map label for this gene is rlmB [H]

Identifier: 93004896

GI number: 93004896

Start: 74042

End: 74812

Strand: Direct

Name: rlmB [H]

Synonym: Pcryo_0065

Alternate gene names: 93004896

Gene position: 74042-74812 (Clockwise)

Preceding gene: 93004895

Following gene: 93004901

Centisome position: 2.42

GC content: 47.47

Gene sequence:

>771_bases
ATGGCAAAACCTACCTATTTTTATGGCATTCATGCCATTGATGCCTTGCTCGAATACCGTCCTCTTGATGGACTGAGCTT
GTTTGTACAGCAAGGGCGTGAGACTGACAGTCATGTACAGTCTATTATGGCGCAAGCACGTGATAATGGCATCAGTATTC
AACCAACGCAAAAAGACAAACTTACTCAGCTGTGTGGTAGCCCGCAACATCAAGGCGTAGTGCTAAACGCCCGTCCTTTA
GGCTTTGCTGATGAAGGTTTGCTAGATACGCTTGCTGGTCGAGATGATTGTTTGTTACTGGTACTAGACCAAATTACGGA
CGCCCATAATTTTGGCGCTTGTTTGCGTACAGCAGTAGCCATGGGTGTCGATGCAGTCATTTGTCCGAAACATCATGCAG
CTAGCTTGACGCCAACGGTTGCCAAAGTATCAGTCGGCGCGGCTGAAATGATGCCTATTGTTAGCGTAACCAATCTGGCA
CGCACGCTGACAAAAATTAAAAATGCCGGCGTTTTTGTGTTTGGTACCGCTCTTAATGCCAATGCCAAACCAATCCATGC
TGCAGATCTGACTGGTAAGACGGCTATTATCATGGGTTCAGAAGGTGAGGGGATGCGTCGCCTGACAACAGAAAGCTGCG
ATGAGCTGGTTTATATTCCGATGTCAGGCAACGAGCATGGCAACTTGCAAAGCTTGAATGTCAGTGTGGCGACAGGTATG
GCTTTGTATGAGATTAATCGGCAGCGAACTTTAGCTGCTGGGCAAGCTTAA

Upstream 100 bases:

>100_bases
ATGAAGCTGGTCATTAATTGCTACAATTACACATCTCTATTTTTATTGCATATTTTTTATAACACTGTGCAATACCCACT
TTATAATCAATAGGCCTATT

Downstream 100 bases:

>100_bases
GATAGTCTTGATGGAGCGGCTCTAATTGTACATATAGGGCCGCAAGATCACTCTCATTTAATACCACATCATCCGCATGA
CGATTGCGCTCTTCACGGCT

Product: RNA methyltransferase TrmH

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MAKPTYFYGIHAIDALLEYRPLDGLSLFVQQGRETDSHVQSIMAQARDNGISIQPTQKDKLTQLCGSPQHQGVVLNARPL
GFADEGLLDTLAGRDDCLLLVLDQITDAHNFGACLRTAVAMGVDAVICPKHHAASLTPTVAKVSVGAAEMMPIVSVTNLA
RTLTKIKNAGVFVFGTALNANAKPIHAADLTGKTAIIMGSEGEGMRRLTTESCDELVYIPMSGNEHGNLQSLNVSVATGM
ALYEINRQRTLAAGQA

Sequences:

>Translated_256_residues
MAKPTYFYGIHAIDALLEYRPLDGLSLFVQQGRETDSHVQSIMAQARDNGISIQPTQKDKLTQLCGSPQHQGVVLNARPL
GFADEGLLDTLAGRDDCLLLVLDQITDAHNFGACLRTAVAMGVDAVICPKHHAASLTPTVAKVSVGAAEMMPIVSVTNLA
RTLTKIKNAGVFVFGTALNANAKPIHAADLTGKTAIIMGSEGEGMRRLTTESCDELVYIPMSGNEHGNLQSLNVSVATGM
ALYEINRQRTLAAGQA
>Mature_255_residues
AKPTYFYGIHAIDALLEYRPLDGLSLFVQQGRETDSHVQSIMAQARDNGISIQPTQKDKLTQLCGSPQHQGVVLNARPLG
FADEGLLDTLAGRDDCLLLVLDQITDAHNFGACLRTAVAMGVDAVICPKHHAASLTPTVAKVSVGAAEMMPIVSVTNLAR
TLTKIKNAGVFVFGTALNANAKPIHAADLTGKTAIIMGSEGEGMRRLTTESCDELVYIPMSGNEHGNLQSLNVSVATGMA
LYEINRQRTLAAGQA

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=221, Percent_Identity=34.3891402714932, Blast_Score=102, Evalue=3e-22,
Organism=Escherichia coli, GI1790623, Length=245, Percent_Identity=43.6734693877551, Blast_Score=199, Evalue=1e-52,
Organism=Escherichia coli, GI1790083, Length=165, Percent_Identity=32.1212121212121, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1788935, Length=249, Percent_Identity=28.5140562248996, Blast_Score=73, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17555908, Length=254, Percent_Identity=26.7716535433071, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324775, Length=196, Percent_Identity=34.6938775510204, Blast_Score=97, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27226; Mature: 27094

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKPTYFYGIHAIDALLEYRPLDGLSLFVQQGRETDSHVQSIMAQARDNGISIQPTQKDK
CCCCEEEEHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCEEECCCCHHH
LTQLCGSPQHQGVVLNARPLGFADEGLLDTLAGRDDCLLLVLDQITDAHNFGACLRTAVA
HHHHHCCCCCCCEEEECCCCCCCCCCHHHHHCCCCCEEEEEEHHHCCCHHHHHHHHHHHH
MGVDAVICPKHHAASLTPTVAKVSVGAAEMMPIVSVTNLARTLTKIKNAGVFVFGTALNA
HCCCEEECCCCCCCCCCCHHHHEECCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECC
NAKPIHAADLTGKTAIIMGSEGEGMRRLTTESCDELVYIPMSGNEHGNLQSLNVSVATGM
CCCCEEEECCCCCEEEEECCCCCCCHHHHHCCCCCEEEEECCCCCCCCEEEEEEEHHHCE
ALYEINRQRTLAAGQA
EEEEECCCCEECCCCC
>Mature Secondary Structure 
AKPTYFYGIHAIDALLEYRPLDGLSLFVQQGRETDSHVQSIMAQARDNGISIQPTQKDK
CCCEEEEHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCEEECCCCHHH
LTQLCGSPQHQGVVLNARPLGFADEGLLDTLAGRDDCLLLVLDQITDAHNFGACLRTAVA
HHHHHCCCCCCCEEEECCCCCCCCCCHHHHHCCCCCEEEEEEHHHCCCHHHHHHHHHHHH
MGVDAVICPKHHAASLTPTVAKVSVGAAEMMPIVSVTNLARTLTKIKNAGVFVFGTALNA
HCCCEEECCCCCCCCCCCHHHHEECCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECC
NAKPIHAADLTGKTAIIMGSEGEGMRRLTTESCDELVYIPMSGNEHGNLQSLNVSVATGM
CCCCEEEECCCCCEEEEECCCCCCCHHHHHCCCCCEEEEECCCCCCCCEEEEEEEHHHCE
ALYEINRQRTLAAGQA
EEEEECCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA